scholarly journals GINGEROL PADA RIMPANG JAHE MERAH (Zingiber officinale, Roscoe) DENGAN METODE PERKOLASI TERMODIFIKASI BASA

2017 ◽  
Vol 1 (2) ◽  
pp. 156
Author(s):  
Lilis Sugiarti ◽  
Asridewi Suwandi ◽  
Amry Syawaalz

Gingerol in red ginger (Zingiber officinale, Roscoe) with percolation method modified base         Ginger was a spice type most widely used in various food and beverage recipes. Ginger is commonly used as a medicine at colds, indigestion, as an analgesic, anti-inflammatory, and others. Some of main components in ginger such as gingerol and shogaol are antioxidants. The purpose of this research was to isolate the red-gingerol in ginger rhizome and to identify. Metode used was extraction process by using percolation with ethanol solvent at room temperature, followed by isolation of gingerol by adding KOH solution at concentrations of 0, 1N, 0.5 N; 1.0 N. Furthermore, the extracted of compounds were identified using TLC and GC-MS.Based on the research result and identification had been carried out on samples of red ginger, it could be concluded that the water content of red ginger samples were 9.70%, with levels of 8.72% oleoresin. The weight of crude gingerol obtained in 1.0 N KOH concentration was to 0.61 g, while the concentration of KOH that produces greatest gingerol was 0.5 N, which amounted of 6.13%. The other peak than the gingerol peak suggested that the isolation was not pure yet. Homovanilil  alcohol  compounds  was  always  in  the greatest  prosentase,  which  was  22%,  followed  by  shogaol  compounds of 4.30% . Ion  with  a value  137 of  m / e:  was  the highest  ions  to be formed and the most stable ion Most compounds isolated by KOH  were phenolic  compounds groups, such as gingerol, shogaol and homovanilil alcohol.Keywords: red ginger, gingerol, extraction, TLC, GC-MS ABSTRAK          Jahe merupakan jenis rempah-rempah yang paling banyak digunakan dalam berbagai resep makanan dan minuman. Jahe biasa digunakan masyarakat sebagai obat masuk angin, gangguan pencernaan, sebagai analgesik, anti-inflamasi, dan lain-lain. Beberapa komponen utama dalam jahe seperti gingerol dan shogaol bersifat antioksidan. Adapun tujuan penelitian ini dilakukan adalah untuk mengisolasi gingerol pada rimpang jahe merah secara optimum dan mengidentifikasinya.Metode penelitian yang digunakan meliputi proses ekstraksi jahe merah dengan menggunakan teknik ekstraksi perkolasi suhu ruang dengan pelarut etanol, dilanjutkan dengan isolasi gingerol dengan penambahan larutan KOH pada konsentrasi 0,1N; 0,5N; 1,0N. Selanjutnya senyawa hasil ekstraksi diidentifikasi dengan menggunakan TLC dan GC-MS. Berdasarkan hasil penelitian dan identifikasi yang telah dilakukan pada sample jahe merah, dapat disimpulkan bahwa kadar air sampel jahe merah yang diteliti adalah sebesar 9,70%, dengan kadar oleoresin sebesar 8,72%. Bobot kasar gingerol terbesar diperoleh pada konsentrasi KOH 1,0N yaitu sebesar 0,61g, sedangkan konsentrasi KOH yang menghasilkan %kemelimpahan gingerol terbesar adalah pada konsentrasi 0,5N, yaitu sebesar 6,13%. Adanya puncak lain selain gingerol menunjukkan bahwa hasil isolasi belum murni. Senyawa homovanilil alkohol selalu terdapat dengan %kemelimpahan terbesar pada setiap sample, yakni 22%, diikuti senyawa shogaol sebesar 4,30%. Ion dengan nilai m/e : 137 adalah ion yang paling banyak terbentuk dan merupakan ion yang stabil. Sebagian besar senyawa yang terisolasi oleh KOH adalah senyawa golongan fenol, seperti gingerol, shogaol dan homovanilil alkohol.Kata kunci : jahe merah, gingerol, ekstraksi, TLC, GC-MS

2021 ◽  
Vol 12 ◽  
Author(s):  
Huanfang Liu ◽  
Honghua Yang ◽  
Tong Zhao ◽  
Canjia Lin ◽  
Yongqing Li ◽  
...  

Ginger (Zingiber officinale Roscoe) is known for its unique pungent taste and useability in traditional Chinese medicine. The main compounds in ginger rhizome can be classified as gingerols, diarylheptanoids, and volatile oils. The composition and concentrations of the bioactive compounds in ginger rhizome might vary according to the age of the rhizome. In this regard, the knowledge on the transcriptomic signatures and accumulation of metabolites in young (Y), mature (M), and old (O) ginger rhizomes is scarce. This study used HiSeq Illumina Sequencing and UPLC-MS/MS analyses to delineate how the expression of key genes changes in Y, M, and O ginger rhizome tissues and how it affects the accumulation of metabolites in key pathways. The transcriptome sequencing identified 238,157 genes of which 13,976, 11,243, and 24,498 were differentially expressed (DEGs) in Y vs. M, M vs. O, and Y vs. O, respectively. These DEGs were significantly enriched in stilbenoid, diarylheptanoid, and gingerol biosynthesis, phenylpropanoid biosynthesis, plant-hormone signal transduction, starch and sucrose metabolism, linoleic acid metabolism, and α-linoleic acid metabolism pathways. The metabolome profiling identified 661 metabolites of which 311, 386, and 296 metabolites were differentially accumulated in Y vs. M, Y vs. O, and M vs. O, respectively. These metabolites were also enriched in the pathways mentioned above. The DEGs and DAMs enrichment showed that the gingerol content is higher in Y rhizome, whereas the Y, M, and O tissues differ in linoleic and α-linoleic acid accumulation. Similarly, the starch and sucrose metabolism pathway is variably regulated in Y, M, and O rhizome tissues. Our results showed that ginger rhizome growth slows down (Y > M > O) probably due to changes in phytohormone signaling. Young ginger rhizome is the most transcriptionally and metabolically active tissue as compared to M and O. The transitioning from Y to M and O affects the gingerol, sugars, linoleic acid, and α-linoleic acid concentrations and related gene expressions.


Author(s):  
Shavilla Lukita ◽  
Winda Khosasi ◽  
Chandra Susanto ◽  
Florenly

Red ginger extract has a category strong antibacterial effect on Staphylococcusaureus and Streptococcus mutans. Red ginger essential oil has the potential forstronger inhibition. This study aims to compare the antibacterial effectiveness of redginger essential oil against Staphylococcus aureus and Streptococcus mutans. Thedesign of this study was a laboratory experimental design with a factorial completelyrandomized design. The red ginger used in this study was proven to be a species ofZingiber officinale Roscoe. The production of essential oils in this study uses thesteam distillation method. The content of secondary metabolites in red ginger wastested quantitatively by the GC-MS method. Determination of antibacterial activityusing the disc diffusion method. The data were processed using the SPSS 21.0program. The normality of data distribution was tested with the Shapiro-Wilk test,followed by one-way ANOVA, Levene's test, and the Tukey HSD Post Hoc Test. Theresults of the antibacterial test of red ginger essential oil against Staphylococcusaureus (21.21mm ± 0.315) and Streptoococcus mutans (23.43mm ± 0.189) provedthat the inhibition power of the category was very strong at a concentration of 75%.


ZOOTEC ◽  
2018 ◽  
Vol 38 (1) ◽  
pp. 160
Author(s):  
Yanter Tahalele ◽  
Martina E.R. Montong ◽  
Fredy J. Nangoy ◽  
Cherlie L. K. Sarajar

THE EFFECT OF ADDITION OF RAMUAN IN DRINKING WATER ON CARCASS PERCENTAGE, ABDOMEN FAT  PERCENTAGE  AND HEART  PERCENTAGE OF AYAM KAMPUNG SUPER. The purpose of this study was to determine the effect of herbs mixed in water added to percentage of carcass, fat abdomen and heart of “ayam kampung super”. This research used a 84 of chicken at five weeks old.The Herbs were garlic (allium sativum l.), kencur (kaempferia galangal l.), turmeric (curcuma domestika val.), temulawak (curcuma xanthorhiza roxb), ginger (zingiber officinale roscoe), leaf leaves (piper better linn) and mahkota dewa (phaleria macrocarpa boerl). The extracted herbs were mixed with palm sugar and fermented with EM4. The complete randomized design consist of 3 treatments and 7 replications were used as methods. The treatment were formulated as follow R0= drinking water without addition of herbs, R1= 1 liter water + 2.5 of mL herbs, R2= 1 liter drinking water + 5 mL of herbs. Research result showed that the addition of herbs 2.5 mL and 5 mL in drinking water shows no significant effect (P >0.05) on the percentage of carcass and liver percentage, but there was an increase in the percentage of carcass in treatment of R1 and R2. While the treatment gave a high significantly effect (P <0.01) on the percentage of abdominal fat. The conclusion of this research is the giving of herbs up to 5 mL added to drinking water does not cause the percentage change of carcass and liver percentage but the giving 5 mL of herbs decrease of abdominal fat percentage of “ayam kampung super”. Key words: Super local chicken, herbs, percentage carcass, percentage of abdominal fat, percentage liver.


2018 ◽  
Vol 2 (2) ◽  
pp. 31-41
Author(s):  
Chairil Anwar ◽  
KM Yahya Syukur ◽  
Dalilah Dalilah ◽  
Salni Salni ◽  
Novrikasari Novrikasari

Aedes aegypti is a vector of Dengue Hemoragic Fever. The use of synthetic chemical insecticide is the most common way to control Ae. aegypti. However, since it can cause resistency if it is used in a wide scale with high frequency and continously, it requires an alternative way. Red ginger (Zingiber officinale Roscoe var. rubrum) is one of natural insecticide which is safe to be used to control Ae. aegypti. This research aims was to determine the activity of active fraction of red ginger to mortality of Ae. aegypti. It was a quasi-experiment research with post test only control design. Twenty Ae. aegypti Liverpool strain were the sample of this research with 5 concentration for each and 4 times repetition. The result showed that concentration 0.5%, 1.0%, 1.5%, 2.0% and 2.5% causing mortality percentage of 32.5%, 33.8%, 51.3%, 58.8% and 65.0% respectively. One way Anova test showed p-value < alpha ± (0.00 < 0.05). It was concluded that there was a significant difference of various concentration of active fraction n-heksan of red ginger extract to the mortality of Ae. aegypti. The lethal concentration (LC50) was 2.409%. It was suggested to continue this research with reppellant or bio-assay test method.   Key words: Toxicity, extract, fraction, Aedes aegypti, Insecticidal


Horticulturae ◽  
2022 ◽  
Vol 8 (1) ◽  
pp. 43
Author(s):  
Dilfuza Jabborova ◽  
Ravish Choudhary ◽  
Abdulahat Azimov ◽  
Zafarjon Jabbarov ◽  
Samy Selim ◽  
...  

Ginger is rich in different chemical compounds such as phenolic compounds, terpenes, polysaccharides, lipids, organic acids, minerals, and vitamins. The present study investigated the effect of mineral fertilizers on the content of mineral elements in the rhizomes of Zingiber officinale Roscoe, soil enzymes activity, and soil properties in Surkhandarya Region, Uzbekistan. To the best of our knowledge, the present study is the first in Uzbekistan to investigate the mineral elements of ginger rhizome inhabiting Termez district, Surkhandarya region. A Field experiment was conducted at the Surkhandarya experimental station research Institute. Four treatments have been studied (Control with no fertilizers (T-1), N75P50K50 kg/ha (T-2), N125P100K100 kg/ha (T-3) and N100P75K75 + B3Zn6Fe6 kg/ha (T-4)). Results showed that T-4 treatment significantly increased ginger rhizome K, Ca, P, Mg, Fe, Na, Mn, Zn, Si, Li, and V content as compared to all other treatments and control. T-3 treatment significantly increased Mo, Ga, and Ag content in comparison to other treatments. Soil enzymes showed a significant increase for all treatments against control, while T-4 treatment has recorded the highest enzyme activity in comparison to all other treatments in urease, invertase, and catalase content. Soil chemical properties have significantly changed for all treatments against the non-cultivated soil and the zero fertilizers plantation with variation among different treatments. Results showed that ginger root is rich in minerals and can be used as a great potential for nutritional supplements and soil enrichment. This study suggest that combination of macro-microelements have the potential to increase the content of mineral elements in the rhizomes of ginger in field conditions.


2021 ◽  
Vol 1 (2) ◽  
pp. 18-25
Author(s):  
Muhammad Nor Arifin Yaakob ◽  
Rasidi Roslan

This work study about the extraction of lignin from Empty Fruit Bunch (EFB). It is a type of lignocellulosic waste produced during the palm oil extraction process. There are three main components of lignocellulosic, which is one of them is lignin. A deep eutectic solvent (DES) with microwave-assisted heating has been used as a process to extract the lignin from EFB and turn it into a value-product. This convenient method was started with the mixing of EFB and DES. After that, the mixture was heated via microwave synthesis reactor at different temperature and time parameters. The extracted lignin yield was dried and ground into a powder form. The highest lignin yield recovered is 30 % by the highest time and temperature. Interestingly, the purity of all lignin yields are above than 80 %. The highest yield of lignin was characterized. According to Fourier-Transform Infrared (FTIR) spectra, there was a significant functional group of phenolic and aliphatic hydroxyl in lignin. Besides, the methoxy group was also configured in lignin spectra. The presence of conjugated alkene also conveyed the characteristic of lignin. The FTIR spectra were intensified with 1H Nuclear Magnetic Resonance (NMR) spectra where there was a chemical shift in lignin and raw EFB which was designated to aliphatic and aromatic protons bonded to a carbon atom. Three regions of decomposition occur in the Thermogravimetric Analysis (TGA) spectra. The initial decompose temperature of lignin was lower compare to raw EFB. Next, second-stage lignin decomposed at 434.14 ℃ with weight loss of 36.21 %. Lastly, for the final stage, lignin decomposes at 552.54 ℃. Moreover, Differential Scanning Calorimetry (DSC) spectra demonstrate that the Tg value of lignin managed to be identified. However, the Tg value of raw EFB cannot be well defined. As for the characterization in residual fractions of EFB, the lowest crystallinity index (CrI) value of raw EFB has proven the presence of lignocellulosic in its structure. The residual fractions that reacted at higher temperatures have an inflated value of CrI as they contain abundant left out cellulose.


2017 ◽  
Vol 4 (2) ◽  
pp. 133
Author(s):  
Hesti Kurniahu

<p><em>PGPR (Plant Growth Promoting Rhizobacteria) is a group of bacteria that colonize in the area of the rhizosphere. These groups of bacteria favor plant growth because they are able to mobilize and provide nutrients and phytohormones. The ability of the bacterial group can be utilized as a growth regulator in the red ginger nursery (Z. officinale Var Rubrum). This study aims to determine the optimal dosage of PGPR for the growth of red ginger seed (Z. officinale Var Rubrum). In this study, red ginger rhizome (Z. officinale Var Rubrum) was soaked with various concentrations of PGPR ie 0%, 25%, 50%, 75% and 100%. The results of this study indicate that soaking rhizomes using PGPR solution significantly influence the growth of red ginger seeds in the form of high seedlings, the number of shoots grow, the number of leaves and the mass of seedlings. A 25% dose has a better ability to boost the growth of seeds when compared to other doses.</em><strong></strong></p><p><em><br /></em><strong></strong></p><p><strong>Keywords: </strong><strong><em>Zingiber officinale Var. Rubrum</em></strong><strong><em>, PGR, Phytohormone</em></strong><strong>. </strong></p>


Author(s):  
Suci Rizkia Amalia ◽  
Iis Rostini ◽  
Mega Laksmini Syamsuddin ◽  
Rusky Intan Pratama

Eucheuma cottonii is one of seaweed species that has beneficial economic value and widely cultivated in Indonesia. Diversifying product into seaweed jelly candy could be carried out to utilize the source. The seaweed distinctive aroma is one of problem in producing jelly candy. Ingredient with strong aroma such as red ginger is needed to covered the smell. This research was aimed to determine the optimum concentration of red ginger (Zingiber officinale Roscoe) extract and seaweed (Eucheuma cottonii) to produce high quality jelly candy based on chemical and organoleptic characteristics. This research used an experimental method consisting of 4 different concentration of red ginger extract (0%, 40%, 50% and 60%) with 20 panelists as evaluators. Chemical composition (water content, protein content, fat content, carbohydrate content, crude fiber content) and organoleptic characteristics (appearance, aroma, texture, taste) of jelly candy were observed as parameters in this research. The results showed that the addition of 50% red ginger extract to jelly candy produced the best organoleptic characteristics and most preferred by panelists. The chemical analysis showed the jelly candy contained 6,22% water, 0,88% protein, 0,19% fat, 96,82% carbohydrate, and 1,54% crude fiber. Addition 50% of red ginger extract is recommended to produce seaweed jelly candy that has the best and most preferred characteristics.


2018 ◽  
Vol 6 (2) ◽  
pp. 205
Author(s):  
Kinanthi Putri Rizki ◽  
Siti Muslichah ◽  
Indah Yulia Ningsih

  This study aimed to determine the effect of the combination of sidaguri leaves and red ginger rhizome extracts in uric acid levels of hyperuricemic male mice. Thirty-six mice were divided into nine groups including normal group, the positive control (+), negative control (-), extracts of four treatment groups with a variety of combinations and two groups of single extract. Hyperuricemia induction was performed by administering mixture melinjo 10% of the standard feed mice for 7 days dan potassium oxonate 250 mg/kg BB intraperitoneally at 2 hours before blood sampling. The results showed that the combination dose of sidaguri leaves of 50 mg/kg BB and red ginger rhizome 400 mg/kg BB had higher antihyperuricemia activity than other combinations in lowering uric acid levels of mice. Compounds may have antihyperuricemic activity were flavonoids, alkaloids, saponins, tannins, and terpenoids.   Keywords: sidaguri leaves, red ginger rhizome, antihyperuricemic activity, uric acid  


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