scholarly journals Protective Effects of Lactobacillus plantarum 16 and Paenibacillus polymyxa 10 Against Clostridium perfringens Infection in Broilers

2021 ◽  
Vol 11 ◽  
Author(s):  
Li Gong ◽  
Baikui Wang ◽  
Yuanhao Zhou ◽  
Li Tang ◽  
Zihan Zeng ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Clostridium Perfringens ◽  
Intestinal Microbiota ◽  
Metabolic Pathways ◽  
Paenibacillus Polymyxa ◽  
Basal Diet ◽  
Control Group ◽  
No Production ◽  
Jejunal Mucosa ◽  
Protective Effects

This study aimed to investigate the protective effects of Lactobacillus plantarum 16 (Lac16) and Paenibacillus polymyxa 10 (BSC10) against Clostridium perfringens (Cp) infection in broilers. A total of 720 one-day-old chicks were randomly divided into four groups. The control and Cp group were only fed a basal diet, while the two treatment groups received basal diets supplemented with Lac16 (1 × 108 cfu·kg−1) and BSC10 (1 × 108 cfu·kg−1) for 21 days, respectively. On day 1 and days 14 to 20, birds except those in the control group were challenged with 1 × 108 cfu C. perfringens type A strain once a day. The results showed that both Lac16 and BSC10 could ameliorate intestinal structure damage caused by C. perfringens infection. C. perfringens infection induced apoptosis by increasing the expression of Bax and p53 and decreasing Bcl-2 expression and inflammation evidence by higher levels of IFN-γ, IL-6, IL-1β, iNOS, and IL-10 in the ileum mucosa, and NO production in jejunal mucosa, which was reversed by Lac16 and BSC10 treatment except for IL-1β (P < 0.05). Besides, the two probiotics restored the intestinal microbiota imbalance induced by C. perfringens infection, characterized by the reduced Firmicutes and Proteobacteria and the increased Bacteroidetes at the phyla level and decreased Bacteroides fragilis and Gallibacterium anatis at the genus level. The two probiotics also reversed metabolic pathways of the microbiota in C. perfringens-infected broilers, including B-vitamin biosynthesis, peptidoglycan biosynthesis, and pyruvate fermentation to acetate and lactate II pathway. In conclusion, Lac16 and BSC10 can effectively protect broilers against C. perfringens infection through improved composition and metabolic pathways of the intestinal microbiota, intestinal structure, inflammation, and anti-apoptosis.

scholarly journals Protective Effects of Bacillus amyloliquefaciens 40 Against Clostridium perfringens Infection in Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Zipeng Jiang ◽  
Wentao Li ◽  
Weifa Su ◽  
Chaoyue Wen ◽  
Tao Gong ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Clostridium Perfringens ◽  
Relative Abundance ◽  
Bacillus Amyloliquefaciens ◽  
Infected Group ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
No Production ◽  
Mrna Levels ◽  
Protective Effects

This study aimed to investigate the protective effects of Bacillus amyloliquefaciens (BA40) against Clostridium perfringens (C. perfringens) infection in mice. Bacillus subtilis PB6 was utilized as a positive control to compare the protective effects of BA40. In general, a total of 24 5-week-old male C57BL/6 mice were randomly divided into four groups, with six mice each. The BA40 and PB6 groups were orally dosed with resuspension bacteria (1 × 109 CFU/ml) once a day, from day 1 to 13, respectively. In the control and infected groups, the mice were orally pre-treated with phosphate-buffered saline (PBS) (200 μl/day). The mice in the infected groups, PB6 + infected group and BA40 + infected group, were orally challenged with C. perfringens type A (1 × 109 CFU/ml) on day 11, whereas the control group was orally dosed with PBS (200 μl/day). The results showed that the BA40 group ameliorated intestinal structure damage caused by the C. perfringens infection. Furthermore, the inflammatory responses detected in the infected groups which include the concentrations of IL-1β, TNF-α, IL-6, and immunoglobulin G (IgG) in the serum and secretory immunoglobulin (SigA) in the colon, and nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) activity in the jejunum, were also alleviated (P < 0.05) by BA40 treatment. Similarly, cytokines were also detected by quantitative PCR (qPCR) in the messenger RNA (mRNA) levels, and the results were consistent with the enzyme-linked immunosorbent assay (ELISA) kits. Additionally, in the infected group, the mRNA expression of Bax and p53 was increasing and the Bcl-2 expression was decreasing, which was reversed by BA40 and PB6 treatment (P < 0.05). Moreover, the intestinal microbiota imbalance induced by the C. perfringens infection was restored by the BA40 pre-treatment, especially by improving the relative abundance of Verrucomicrobiota (P < 0.05) and decreasing the relative abundance of Bacteroidetes (P < 0.05) in the phyla level, and the infected group increased the relative abundance of some pathogens, such as Bacteroides and Staphylococcus (P < 0.05) in the genus level. The gut microbiota alterations in the BA40 group also influenced the metabolic pathways, and the results were also compared. The purine metabolism, 2-oxocarboxylic acid metabolism, and starch and sucrose metabolism were significantly changed (P < 0.05). In conclusion, our results demonstrated that BA40 can effectively protect mice from C. perfringens infection.

scholarly journals In vitro protective effects of Lactobacillus plantarum Lac16 on Clostridium perfringens infection-associated intestinal injury in IPEC-J2 cells

2021 ◽  
Author(s):  
Yuanhao Zhou ◽  
Baikui Wang ◽  
Qi Wang ◽  
Li Tang ◽  
Peng Zou ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Lactobacillus Plantarum ◽  
Clostridium Perfringens ◽  
Intestinal Injury ◽  
Mitogen Activated Protein Kinase ◽  
Epithelial Cell Line ◽  
Protective Effects ◽  
Host Defense Peptides ◽  
Expression Levels ◽  
Mrna Expression Levels

Abstract Background: Clostridium perfringens causes intestinal injury through overgrowth and secretion of multiple toxins, leading to diarrhea and necrotic enteritis in animals, such as pigs. Lactobacillus plantarum (L. plantarum) Lac16 has been reported to protect broilers against C. perfringens infection. This study aimed at investigating the protective effects of Lactobacillus plantarum Lac16 on C. perfringens infection-associated intestinal injury in intestinal porcine epithelial cell line (IPEC-J2). Results: The results showed that L. plantarum Lac16 significantly inhibit the growth and biofilm formation of C. perfringens (P < 0.001). In the co-culture system, L. plantarum Lac16 significantly suppressed colony forming units (CFU) of C. perfringens (P < 0.05), which was accompanied by a decrease in pH levels (P < 0.01). Moreover, L. plantarum Lac16 significantly elevated the mRNA expression levels of host defense peptides (HDPs) in IPEC-J2 cells (P < 0.05), decreased C. perfringens-induced cellular cytotoxicity (P < 0.01) and adhesion to cells (P < 0.05). At the same time, L. plantarum Lac16 significantly attenuated C. perfringens-induced damage to intestinal barrier integrity and the decrease in claudin-1 (P < 0.01) as well as zona occludens 1 (ZO-1) expressions. Preincubation with L. plantarum Lac16 significantly suppressed mRNA expression levels of pattern recognition receptors (PRRs) (Toll-like receptor (TLR) 1, TLR2, nucleotide-binding oligomerization domain (NOD) 1) in C. perfringens-challenged IPEC-J2 cells (P < 0.01). C. perfringens significantly elevated the phosphorylation of p38 mitogen-activated protein kinase (MAPK), JNK, and p65 nuclear factor-κB (NF-κB) (P < 0.05) while L. plantarum Lac16 pre-incubation effectively inhibited phosphorylation of p65 (P < 0.001). Furthermore, L. plantarum Lac16 significantly suppressed C. perfringens induced gene expressions of proinflammatory cytokines (interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor-α (TNF-α)) (P < 0.05). Conclusions: Collectively, probiotic L. plantarum Lac16 exerts protective effects against C. perfringens infection-associated intestinal injury in IPEC-J2 cells.

scholarly journals Sodium Humate And Glutamine Combined Supplementation Alleviate Diarrhea of Weaned Calves Via Alter Intestinal Microbiota And Metabolites

2021 ◽  
Author(s):  
Dong Wang ◽  
Yuanyi Du ◽  
Haotian Jia ◽  
Siqi Huang ◽  
Lei Lei ◽  
...  
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Intestinal Microbiota ◽  
Basal Diet ◽  
Intestinal Microbiome ◽  
Control Group ◽  
Diarrhea Incidence ◽  
Combined Supplementation ◽  
Similar Body ◽  
Fecal Score

Abstract Background: Weaning is one of the most stressful periods that cause gastrointestinal tract dysfunction and diarrhea in calves. HNa and Gln were reported to exert beneficial effects on promoting growth performance, decreasing diarrhea incidence, and modulating intestinal microbiota in animals. Therefore, this study investigated the effect of HNa and Gln combined supplementation on growth performance, diarrhea incidence, serum parameters, intestinal microbiome, and metabolites of weaned calves.Results: In Exp. 1, 40 calves at 51±3 days of age with similar body weight (66.82±4.31 kg) were randomly assigned to 4 treatments fed with a basal diet (NC group), and a basal diet supplemented with 100 mL of 1%, 3%, or 5% HNa+1% Gln, twice daily, respectively. In a 21-day trial, calves on the 5% HNa+1% Gln group had higher ADG and lower fecal score and diarrhea incidence than those in the control group (P < 0.05). In Exp. 2, 20 calves at 51±3 days of age with similar body weight (69.37±6.28 kg) were randomly assigned to 2 treatments fed with a basal diet (NC group) and a basal diet supplemented with 100 mL of 5% HNa+1% Gln, twice daily (H+G group, the dose was obtained from Exp. 1). In a 21-day trial, calves supplemented with HNa and Gln had higher final BW and ADG, serum IgG concentration and GSH-Px and T-AOC activities, but lower fecal score, diarrhea incidence, as well as serum DAO, D-lac, TNF-α, and MDA concentrations compared to NC group (P < 0.05). Analysis of intestinal microbiota indicated that supplemented with HNa and Gln significantly increased the abundance of phyla Firmicutes and genus of Bifidobacterium, Lactobacillus, Olsenella, Ruminiclostridium 9, Howardella, and uncultured organism, whereas the abundance of phyla Bacteroidetes, genus of Helicobacter and Lachnoclostridium were decreased as compared with NC group. Moreover, untargeted metabolomics analysis revealed that supplemented with HNa and Gln altered 18 metabolites and enriched 6 KEGG pathways (primary fatty acid biosynthesis) compared to the NC group. Conclusions: This study showed that combined supplemented with HNa and Gln could decrease diarrhea of weaned calves, which may be associated with improved intestinal microbial ecology and altered metabolism profile.

scholarly journals Protective Effects of Lactobacillus plantarum Lac16 on Clostridium perfringens Infection-Associated Injury in IPEC-J2 Cells

2021 ◽  
Vol 22 (22) ◽  
pp. 12388
Author(s):  
Yuanhao Zhou ◽  
Baikui Wang ◽  
Qi Wang ◽  
Li Tang ◽  
Peng Zou ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Clostridium Perfringens ◽  
Host Defense ◽  
Intestinal Barrier ◽  
Epithelial Cell Line ◽  
Necrotic Enteritis ◽  
Protective Effects ◽  
Gene Expressions ◽  
Host Defense Peptides ◽  
Associated Injury

Clostridium perfringens (C. perfringens) causes intestinal injury through overgrowth and the secretion of multiple toxins, leading to diarrhea and necrotic enteritis in animals, including pigs, chickens, and sheep. This study aimed to investigate the protective effects of Lactobacillus plantarum (L. plantarum) Lac16 on C. perfringens infection-associated injury in intestinal porcine epithelial cell line (IPEC-J2). The results showed that L. plantarum Lac16 significantly inhibited the growth of C. perfringens, which was accompanied by a decrease in pH levels. In addition, L. plantarum Lac16 significantly elevated the mRNA expression levels of host defense peptides (HDPs) in IPEC-J2 cells, decreased the adhesion of C. perfringens to IPEC-J2 cells, and attenuated C. perfringens-induced cellular cytotoxicity and intestinal barrier damage. Furthermore, L. plantarum Lac16 significantly suppressed C. perfringens-induced gene expressions of proinflammatory cytokines and pattern recognition receptors (PRRs) in IPEC-J2 cells. Moreover, L. plantarum Lac16 preincubation effectively inhibited the phosphorylation of p65 caused by C. perfringens infection. Collectively, probiotic L. plantarum Lac16 exerts protective effects against C. perfringens infection-associated injury in IPEC-J2 cells.

scholarly journals Diversity In Intestinal Microorganisms Contributes To The Bodysize Difference In Chinese Horses And Ponies

2021 ◽  
Author(s):  
Shipeng Lv ◽  
Yanli Zhang ◽  
Zhengkai Zhang ◽  
Sihan Meng ◽  
Yabin Pu ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Metabolic Pathways ◽  
Control Group ◽  
Cellulose Decomposition ◽  
Intestinal Microorganisms ◽  
Horse Breeding ◽  
Body Development ◽  
Rich Diversity ◽  
Rrna Sequences ◽  
16S Rrna Sequences

Abstract Background: Intestinal microbiota communities can reflect the digestion and metabolism of the host, as well as the appearance of the host. In China, there are various excellent horse and pony breeds with rich diversity in wither height. However, little is known about the community structure of the intestinal microbiota in horses, let alone the profound effects it causes. Results: Here in, we generated 16S rRNA sequences of intestinal microorganisms from 118 Chinese horses including Guanzhong horse, Debao pony, and Ningqiang pony. We found that the intestinal microbiota of horses is full of diversity, and Firmicutes, Bacteroidetes, and Spirochaetes, which is consistent with the special structure of the horse digestive tract. Interestingly, the abundance of Firmicutes and Bacteroidetes at the phylum level, showed a strong correlation with horse height, with R values of 0.82 and -0.86 respectively. Moreover, at the genus level, Coprococcus, Streptococcus, Treponema, and Prevotella demonstrated higher significance in terms of height, the prediction of PICRUSt2 function and multiple analyses of the metabolic pathways, and additionally, the metabolic pathways of energy intake and utilization were significantly enriched in horses relative to ponies (P<0.01). Notably, flora colonization in mouse littermates contributed to their broad development compared to the control group. Conclusions: Compared with ponies, the intestinal microbiota enabled better cellulose decomposition and energy uptake in horses; Thus horses could get more energy from food to meet their higher demand for larger body development than ponies. Therefore, our study helps to understand the gut microbiota patterns across equine breeds, which has the potential to advance approaches aimed at personalized microbial modifications in horse breeding.

scholarly journals The Protective Effects of Chronic Intermittent Hypobaric Hypoxia Against Osteoarthritis in Rats - Role of Nitric Oxide

2021 ◽  
Author(s):  
Tianci Wang ◽  
Dong Ren ◽  
Yunshan Su ◽  
Jian Lu ◽  
Ming Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Hypobaric Hypoxia ◽  
Meniscal Tear ◽  
Cartilage Degeneration ◽  
Joint Fluid ◽  
Control Group ◽  
No Production ◽  
Protective Effects ◽  
Blank Control Group ◽  
Intermittent Hypobaric Hypoxia

Abstract The study explored the effect of chronic intermittent hypobaric hypoxia (CIHH) on osteoarthritis (OA). CIHH conditioning was realized by exposing rats to hypobaric hypoxia environment mimicking 5,000 m high-altitude (PB=404 mmHg, PO2=84 mmHg) 6 h per day, once daily for 28 days. OA model was induced by surgically-induced medial meniscal tear. Male Wistar rats were randomly assigned into 5 groups: preconditioning group （CIHH + OA）, postconditioning group （OA + CIHH）, control group, inhibitor group [OA + inducible nitric oxide synthase (iNOS) inhibitor], blank control group. The expression iNOS, nitric oxide (NO) content levels in the joint fluid were measured at 1, 2, 3 weeks after the OA modelling. Results revealed that OA modelling induced cartilage degeneration, up-regulated iNOS expression, increased joint fluid NO content. CIHH preconditioning and postconditioning reduced cartilage degeneration, prevented the NO production. Inhibitor groups showed alleviated joint degeneration than control group, but not as effective as CIHH condition. These results suggest that both CIHH preconditioning and postconditioning plays a protective role on OA, one of the mechanism was inhibiting the overexpression of iNOS and NO production.

scholarly journals Dietary Cold Pressed Watercress and Coconut Oil Mixture Enhances Growth Performance, Intestinal Microbiota, Antioxidant Status, and Immunity of Growing Rabbits

Animals ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 212 ◽  
Author(s):  
Mahmoud Alagawany ◽  
Mohamed Abd El-Hack ◽  
Adham Al-Sagheer ◽  
Mohammed Naiel ◽  
Islam Saadeldin ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Antioxidant Status ◽  
Pathogenic Bacteria ◽  
Coconut Oil ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Control Group ◽  
Feed Conversion ◽  
Live Body Weight ◽  
Cold Pressed

The present study assessed the effect of dietary supplementation with coconut oil (CNO), watercress oil (WCO), and their mixture as promoters of growth, antioxidant status, immunity, and intestinal microbiota in growing rabbits. A total of 120 rabbits were distributed into six groups (20 rabbits/group) receiving a basal diet without supplementation (G1) or diet supplemented with 2 g CNO (G2), 2 g WCO (G3), 0.5 g CNO plus 1.5 g WCO (G4), 1 g CNO plus 1 g WCO (G5), or 1.5 g CNO plus 0.5 g WCO/kg (G6). Live body weight and feed conversion ratio were significantly higher in the G4 and G5 groups than in the other groups. Superoxide dismutase activity and reduced glutathione concentration were significantly improved in the CNO or WCO diets. Supplemental CNO plus WCO at all tested levels produced the best lysozyme and complement 3 activities. Cecal lactobacilli, coliform, Enterobacteriaceae, and Clostridium spp. populations were lower in the group who received the 1 g CNO + 1 g WCO/kg diet than that in the control group. Dietary supplementation of 1 g CNO + 1 g WCO or 0.5 g CNO + 1.5 g WCO/kg had the potential to improve growth, feed utilization, antioxidant status, and immunity, and reduce cecal pathogenic bacteria in rabbits.

scholarly journals Cinnamon Extract and Probiotic Supplementation Alleviate Copper-Induced Nephrotoxicity via Modulating Oxidative Stress, Inflammation, and Apoptosis in Broiler Chickens

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1609
Author(s):  
Sara T. Elazab ◽  
Nahla S. Elshater ◽  
Asmaa T. Y. Kishaway ◽  
Huda A. EI-Emam
Keyword(s):  
Mrna Expression ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Serum Levels ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Protective Effects ◽  
Renal Protection ◽  
Necrosis Factor Alpha ◽  
Cinnamon Extract

The present study aimed to assess the potential protective effects of cinnamon (Cinnamomum zeylanicum, Cin) and probiotic against CuSO4-induced nephrotoxicity in broiler chickens. One-day-old Cobb chicks were assigned into seven groups (15 birds/group): control group, fed basal diet; Cin group, fed the basal diet mixed with Cin (200 mg/kg); PR group, receiving PR (1 g/4 L water); Cu group, fed the basal diets mixed with CuSO4 (300 mg/kg); Cu + Cin group; Cu + PR group; and Cu + Cin + PR group. All treatments were given daily for 6 weeks. Treatment of Cu-intoxicated chickens with Cin and/or PR reduced (p < 0.05) Cu contents in renal tissues and serum levels of urea, creatinine, and uric acid compared to the Cu group. Moreover, Cin and PR treatment decreased lipid peroxidation and increased antioxidant enzyme activities in chickens’ kidney. Additionally, significant reduction (p < 0.05) in the mRNA expression of tumor necrosis factor alpha (TNF-α), interleukin (IL-2) and Bax, and in cyclooxygenase (COX-II) enzyme expression, and significant elevation (p < 0.05) in mRNA expression of IL-10 and Bcl-2 were observed in kidneys of Cu + Cin, Cu + PR, and Cu + Cin + PR groups compared to Cu group. Conclusively, Cin and/or PR afford considerable renal protection against Cu-induced nephrotoxicity in chickens.

scholarly journals Alterations in Intestinal Microbiota Composition in Mice Treated With Vitamin D3 or Cathelicidin

2021 ◽  
Vol 11 ◽  
Author(s):  
Yu Jiang ◽  
Yue Wan ◽  
Jing Li ◽  
Yueshui Zhao ◽  
Yongshun Ma ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Antimicrobial Peptide ◽  
Intestinal Microbiota ◽  
Vitamin D3 ◽  
Metabolic Pathways ◽  
Antimicrobial Activities ◽  
Protective Effects ◽  
Rrna Sequencing ◽  
The Impact ◽  
Harmful Effects

Gut microbiota is a complex aggregation of microbial organisms, which offers diverse protective benefits to the host. Dysbiosis of intestinal microbiota is frequently associated with many diseases. Vitamin D3 (VD), which was originally associated with bone health, also possesses antimicrobial activities and can act through antimicrobial peptide. Cathelicidin is a type of antimicrobial peptide in host to maintain the balance of gut microbiome. Our current study sought to evaluate the protective effect of VD and cathelicidin in mice intestines by administration of VD or mCRAMP-encoding L. lactis. We herein provided a comprehensive profile of the impact of VD and mCRAMP on gut microbiota using 16S rRNA sequencing, followed by bioinformatics and statistical analysis. Our results revealed an increased richness of bacterial community in mice intestines due to VD administration. Moreover, we showed a beneficial effect of VD and mCRAMP by enhancing the colonization of bacterial taxa that are associated with protective effects to the host but repressing the propagation of bacterial taxa that are associated with harmful effects to the host. Various metabolic pathways related to amino acid and lipid metabolism were affected in this process. We further established a bacterial panel as a reliable biomarker to evaluate the efficacy of remodeling the mice gut microbiota by VD and mCRAMP administration. The uncovered effects will deepen the comprehension about the antibacterial mechanisms of VD and mCRAMP and provide new insights for therapeutic implication of them.

scholarly journals Protective effect of vitamin E on sperm parameters, chromatin quality, and DNA fragmentation in mice treated with different doses of ethanol: An experimental study

2021 ◽  
Author(s):  
Mohamad Reza Doostabadi ◽  
Mohammadmehdi Hassanzadeh-taheri ◽  
Mahmoud Asgharzadeh ◽  
Masoomeh Mohammadzadeh
Keyword(s):  
Vitamin E ◽  
Dna Fragmentation ◽  
Sperm Quality ◽  
Dna Structure ◽  
Basal Diet ◽  
Sperm Parameters ◽  
Sperm Chromatin ◽  
Control Group ◽  
Protective Effects ◽  
Normal Morphology

Background: Excessive consumption of alcohol induces an increase in oxidative stress production and can lead to detrimental effects on the male reproductive system. Objective: To evaluate the possible protective effects of coadministration of vitamin (vit) E on the detrimental changes in the sperm quality of mice administered ethanol. Materials and Methods: Fifty-four BALB/c mice were categorized into nine groups (n = 6/each). The control group received a basal diet while the eight experimental groups received ethanol 10%; ethanol 20%; vit. E 100 mg; vit. E 200 mg; ethanol 10% + vit. E 100 mg; ethanol 10% + vit. E 200 mg; ethanol 20% + vit. E 100 mg; ethanol 20% + vit. E 200 mg. After 35 days, the sperm parameters and sperm chromatin were assessed. Results: The results demonstrated a significant reduction in the motility rate, normal morphology rate, viability rate, increase in abnormal DNA structure and packaging (TB staining), and DNA damage (TUNEL) in ethanol consumer groups. In addition, the findings showed a significant increase in the aforementioned parameters in ethanoland vit. E-consumer groups compared to the ethanol-only consumer groups. The ethanol group received 20% of the most damage among the groups. The group receiving vit. E 100 mg and those receiving ethanol 10% + vit. E 200 mg gained the highest benefit among the groups. Conclusion: Sperm forward progressive motility, normal morphology rate, and viability decreased in the ethanol groups. Also, the rates of spermatozoa with abnormal DNA structure and DNA fragmentation increased in the ethanol groups. Our findings revealed that the coadministration of vit. E and ethanol can protect destructive changes in DNA structure and damage. Key words: Ethanol, Sperm parameters, Vitamin E.

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