scholarly journals Comparative Characterization of Eastern Carrot Accessions for Some Main Agricultural Traits

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2460
Author(s):  
José R. Acosta-Motos ◽  
Pedro Díaz-Vivancos ◽  
Verónica Becerra-Gutiérrez ◽  
José A. Hernández Cortés ◽  
Gregorio Barba-Espín
Keyword(s):  
Leaf Area ◽  
High Yield ◽  
Nutrient Concentrations ◽  
Composition Analysis ◽  
Anthocyanin Content ◽  
Black Carrot ◽  
Comparative Characterization ◽  
Coloration Patterns ◽  
Promising Source ◽  
First Time

Background: Unevaluated open-pollinated germplasm represents a promising source of variability to face the problems of worldwide food production under a changing environment. In carrots, this is particularly true for black carrot accessions, which are the most relevant among Eastern carrot germplasm due to their high anthocyanin content and, concomitantly, antioxidant capacity. Methods: In the present work, a comparative characterization was conducted for the first time on 11 Eastern carrot landraces and Night Bird ‘F1’ as the reference cultivar, grown under glasshouse conditions at temperatures up to 33 °C. Results: Some landraces showed their potential for ulterior evaluation in terms of plant and taproot size, plant compactness, specific leaf area and leaf area ratio, among other traits. The highest anthocyanin and flavonoid contents were found in the reference cultivar, whereas remarkable differences in these variables were observed for the rest of accessions, which in turn may correlate with very distinct coloration patterns. Premature bolting and taproot shape abnormalities were also recorded. Mineral composition analysis showed the nutritional potential of Eastern carrot leaves, which displayed higher concentration than taproot tissue for several macro- and micronutrients. Moreover, several accessions had higher nutrient concentrations than the reference cultivar, which also highlights their profitability. Conclusions: This work contributes to the knowledge on Eastern black carrot germplasm by characterizing some of its main agricultural traits, and opens up the prospect for complementary evaluation on high-yield accessions.

The Smoulder Stream of Cigarettes / Zur Kenntnis des Glimmstromes von Cigaretten

1965 ◽  
Vol 3 (2) ◽  
Author(s):  
F. Seehofer ◽  
W. Schulz
Keyword(s):  
Moisture Content ◽  
Spatial Position ◽  
High Yield ◽  
Physical Conditions ◽  
Moisture Contents ◽  
Cigarette Paper ◽  
First Time

AbstractThe phenomenon of the smoulder stream flowing through the cigarette during smouldering and during the puff intervals is demonstrated for the first time and its dependence upon physical conditions is examined. The volume of the smoulder stream can amount up to 180 ml per cigarette. Increasing draw resistance of the cigarette and augmenting moisture content of the tobacco as well as perforation of the cigarette paper have a decreasing effect on volume and velocity of the smoulder stream. The porosity of the cigarette paper has no perceptible influence. The spatial position of the cigarette affects volume and velocity of the smoulder stream. The influence exercised by the smoulder stream on the yields of total condensate, nicotine, phenols, aldehydes, and acroleine when the cigarette tip is open during the puff intervals is determined. When the moisture contents of the tobacco were extremely high, yield decreases reaching 50 % could be observed.

Iridium-Catalyzed Silylation of C-H bonds in Unactivated Arenes: A Sterically-Encumbered Phenanthroline Ligand Accelerates Catalysis Enabling New Reactivity

2019 ◽  
Author(s):  
Caleb Karmel ◽  
Zhewei Chen ◽  
John Hartwig
Keyword(s):  
High Activity ◽  
Functional Group ◽  
Activity Analysis ◽  
High Reactivity ◽  
High Yield ◽  
Phen Ligand ◽  
Mechanistic Studies ◽  
Phenanthroline Ligand ◽  
First Time ◽  
New System

We report a new system for the silylation of aryl C-H bonds. The combination of [Ir(cod)(OMe)]<sub>2</sub> and 2,9-Me<sub>2</sub>-phenanthroline (2,9-Me<sub>2</sub>phen) catalyzes the silylation of arenes at lower temperatures and with faster rates than those reported previously, when the hydrogen byproduct is removed, and with high functional group tolerance and regioselectivity. Inhibition of reactions by the H<sub>2</sub> byproduct is shown to limit the silylation of aryl C-H bonds in the presence of the most active catalysts, thereby masking their high activity. Analysis of initial rates uncovered the high reactivity of the catalyst containing the sterically hindered 2,9-Me<sub>2</sub>phen ligand but accompanying rapid inhibition by hydrogen. With this catalyst, under a flow of nitrogen to remove hydrogen, electron-rich arenes, including those containing sensitive functional groups, undergo silylation in high yield for the first time, and arenes that underwent silylation with prior catalysts react over much shorter times with lower catalyst loadings. The synthetic value of this methodology is demonstrated by the preparation of key intermediates in the synthesis of medicinally important compounds in concise sequences comprising silylation and functionalization. Mechanistic studies demonstrate that the cleavage of the aryl C-H bond is reversible and that the higher rates observed with the 2,9-Me<sub>2</sub>phen ligand is due to a more thermodynamically favorable oxidative addition of aryl C-H bonds.

scholarly journals Bacterial cellulose spheroids as building blocks for 3D and patterned living materials and for regeneration

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Joaquin Caro-Astorga ◽  
Kenneth T. Walker ◽  
Natalia Herrera ◽  
Koon-Yang Lee ◽  
Tom Ellis
Keyword(s):  
Bacterial Cellulose ◽  
Building Blocks ◽  
Genetically Engineered ◽  
High Yield ◽  
Synthetic Materials ◽  
Chemical Inputs ◽  
First Time ◽  
3D Shapes ◽  
Promising Avenue ◽  
Living Materials

AbstractEngineered living materials (ELMs) based on bacterial cellulose (BC) offer a promising avenue for cheap-to-produce materials that can be programmed with genetically encoded functionalities. Here we explore how ELMs can be fabricated in a modular fashion from millimetre-scale biofilm spheroids grown from shaking cultures of Komagataeibacter rhaeticus. Here we define a reproducible protocol to produce BC spheroids with the high yield bacterial cellulose producer K. rhaeticus and demonstrate for the first time their potential for their use as building blocks to grow ELMs in 3D shapes. Using genetically engineered K. rhaeticus, we produce functionalized BC spheroids and use these to make and grow patterned BC-based ELMs that signal within a material and can sense and report on chemical inputs. We also investigate the use of BC spheroids as a method to regenerate damaged BC materials and as a way to fuse together smaller material sections of cellulose and synthetic materials into a larger piece. This work improves our understanding of BC spheroid formation and showcases their great potential for fabricating, patterning and repairing ELMs based on the promising biomaterial of bacterial cellulose.

scholarly journals A Polyphenol Enriched Variety of Apple Alters Circulating Immune Cell Gene Expression and Faecal Microbiota Composition in Healthy Adults: A Randomized Controlled Trial

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1092
Author(s):  
Matthew P. G. Barnett ◽  
Wayne Young ◽  
Kelly Armstrong ◽  
Diane Brewster ◽  
Janine M. Cooney ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Cell ◽  
Controlled Trial ◽  
Composition Analysis ◽  
Cell Mediated Immunity ◽  
Anthocyanin Content ◽  
Microbiota Composition ◽  
Faecal Microbiota ◽  
Apple Variety ◽  
Relative Abundances

Polyphenols within fruits and vegetables may contribute to health benefits due to their consumption, with the anthocyanin sub-set also adding colour. The Lemonade™ apple variety has green skin and white flesh, with low anthocyanin content, while some apple varieties have high anthocyanin content in both the skin and flesh. Effects of red compared with white-fleshed apples were studied in healthy human subjects in a randomized, placebo-controlled, cross-over intervention trial. Twenty-five healthy subjects consumed dried daily portions of the red-fleshed or placebo (white-fleshed) apple for two weeks, followed by one-week washout and further two-week crossover period. During the study, volunteers provided faecal samples for microbiota composition analysis and blood samples for peripheral blood mononuclear cell (PBMC) gene expression analysis. Subtle differences were observed in the faecal microbiota of subjects that were fed the different apples, with significant (p < 0.05) reductions in relative abundances of Streptococcus, Ruminococcus, Blautia, and Roseburia, and increased relative abundances of Sutterella, Butyricicoccus, and Lactobacillus in subjects after consuming the red apple. Changes in PBMC gene expression showed 18 mRNA transcripts were differentially expressed between the two groups, of which 16 were immunoglobulin related genes. Pathway analysis showed that these genes had roles in pathways such as immunoglobulin production, B cell-mediated immunity, complement activation, and phagocytosis. In conclusion, this study shows that anthocyanin-rich apples may influence immune function compared to control apples, with changes potentially associated with differences in the faecal microbiota.

scholarly journals Constituents of Chamaecrista diphylla (L.) Greene Leaves with Potent Antioxidant Capacity: A Feature-Based Molecular Network Dereplication Approach

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 681
Author(s):  
Paulo Gomes ◽  
Luis Quirós-Guerrero ◽  
Abraão Muribeca ◽  
José Reis ◽  
Sônia Pamplona ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Ethanolic Extract ◽  
Oxygen Radical ◽  
Molecular Network ◽  
Antioxidant Compounds ◽  
Herbaceous Plant ◽  
Network Approach ◽  
Feature Based ◽  
Promising Source ◽  
First Time

Chamaecrista diphylla (L.) Greene (Fabaceae/Caesalpiniaceae) is a herbaceous plant that is widely distributed throughout the Americas. Plants from this genus have been used in traditional medicine as a laxative, to heal wounds, and to treat ulcers, snake and scorpion bites. In the present study, we investigated the chemical composition of Chamaecrista diphylla leaves through a mass spectrometry molecular network approach. The oxygen radical absorbance capacity (ORAC) for the ethanolic extract, enriched fractions and isolated compounds was assessed. Overall, thirty-five compounds were annotated for the first time in C. diphylla. Thirty-two of them were reported for the first time in the genus. The isolated compounds 9, 12, 24 and 33 showed an excellent antioxidant capacity, superior to the extract and enriched fractions. Bond dissociation energy calculations were performed to explain and sustain the antioxidant capacity found. According to our results, the leaves of C. diphylla represent a promising source of potent antioxidant compounds.

scholarly journals A Novel and Efficient High-Yield Method for Preparing Bacterial Ghosts

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 420
Author(s):  
Yi Ma ◽  
Liu Cui ◽  
Meng Wang ◽  
Qiuli Sun ◽  
Kaisheng Liu ◽  
...  
Keyword(s):  
Large Scale ◽  
Expression System ◽  
High Yield ◽  
Wild Type ◽  
High Quality ◽  
Efficient Protection ◽  
Bacterial Ghosts ◽  
Cell Envelopes ◽  
Extracellular Structures ◽  
First Time

Bacterial ghosts (BGs) are empty cell envelopes possessing native extracellular structures without a cytoplasm and genetic materials. BGs are proposed to have significant prospects in biomedical research as vaccines or delivery carriers. The applications of BGs are often limited by inefficient bacterial lysis and a low yield. To solve these problems, we compared the lysis efficiency of the wild-type protein E (EW) from phage ΦX174 and the screened mutant protein E (EM) in the Escherichia coli BL21(DE3) strain. The results show that the lysis efficiency mediated by protein EM was improved. The implementation of the pLysS plasmid allowed nearly 100% lysis efficiency, with a high initial cell density as high as OD600 = 2.0, which was higher compared to the commonly used BG preparation method. The results of Western blot analysis and immunofluorescence indicate that the expression level of protein EM was significantly higher than that of the non-pLysS plasmid. High-quality BGs were observed by SEM and TEM. To verify the applicability of this method in other bacteria, the T7 RNA polymerase expression system was successfully constructed in Salmonella enterica (S. Enterica, SE). A pET vector containing EM and pLysS were introduced to obtain high-quality SE ghosts which could provide efficient protection for humans and animals. This paper describes a novel and commonly used method to produce high-quality BGs on a large scale for the first time.

scholarly journals Effect of shoot topping intensity on 'Cabernet Franc' grapevine maturity in high-altitude region

2017 ◽  
Vol 52 (10) ◽  
pp. 946-950
Author(s):  
Douglas André Würz ◽  
José Luiz Marcon Filho ◽  
Alberto Fontanella Brighenti ◽  
Ricardo Allebrandt ◽  
Betina Pereira de Bem ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Leaf Area ◽  
Soluble Solids ◽  
Anthocyanin Content ◽  
Total Polyphenol ◽  
Cabernet Franc ◽  
Grape Berries ◽  
Grape Quality ◽  
Vegetative Canopy ◽  
Different Levels

Abstract: The objective of this work was to evaluate the chemical composition of 'Cabernet Franc' grape subjected to different levels of shoot topping. The experiment was conducted in the 2008 and 2009 vintages, and the levels of shoot topping consisted of: control, without topping; and reductions of 15, 30, and 45% in total leaf area. The effect of shoot topping intensity on the chemical composition of the 'Cabernet Franc' grape berries was observed. A reduction of 26% in the vegetative canopy through a moderate shoot topping increased the total polyphenol index and the anthocyanin content. Shoot topping is a practice that must be carried out with caution, since severe shoot topping, i.e., a reduction greater than 26% of the leaf area, may negatively impact grape quality by reducing the content of soluble solids and decreasing the total polyphenol index and the content of anthocyanins of 'Cabernet Franc' grape berries.

scholarly journals Assessment of costs on the adaptation to climate change in Ukraine

2020 ◽  
Vol 2020 (4) ◽  
pp. 60-71
Author(s):  
Galyna Trypolska ◽  
◽  
Keyword(s):  
Climate Change ◽  
Water Protection ◽  
Adaptation To Climate Change ◽  
Adaptation Measures ◽  
Global Environmental ◽  
The Cost ◽  
Adaptive Nature ◽  
Regional Budgets ◽  
Promising Source ◽  
First Time

The paper focuses on the main adaptation measures financed in Ukraine at the expense of state and regional budgets region-wise during 2016-2018 within the framework of environmental activities. The source of data was "Environmental passports of the regions". The paper finds that during 2016-2018 the most funded items of expenditure were the construction and repair of sewerage, clearing of riverbeds, repair of water protection structures and wastewater treatment. A significant gap in the cost of measures of an adaptive nature between the regions is established. Expenditures in the Transcarpathian region, which most suffers from floods, were the lowest: they were 46 times lower than expenditures on adaptation measures in the Dnipropetrovsk region, which actively allocated funds for clearing riverbeds and strengthening the river banks. During 2016-2018, almost USD 110 million were proved to be spent on adaptation measures in Ukraine. The main source of funds were local budgets, in particular regional environmental funds. Green bonds are determined to become a promising source of funding for adaptation measures in Ukraine. For the first time, the amount of funds required for adaptation measures in Ukraine until 2050 has been partially estimated. It is proved that the only international funds that can provide funding for adaptation measures in Ukraine in late 2020, is the Global Environmental Facility.

scholarly journals Growth of and partitioning between shoot and storage root of carrot in a northern climate

2000 ◽  
Vol 9 (1) ◽  
pp. 49-59 ◽  
Author(s):  
T. SUOJALA
Keyword(s):  
Leaf Area ◽  
Growing Season ◽  
Climatic Conditions ◽  
Yield Potential ◽  
High Yield ◽  
Root Weight ◽  
Leaf Production ◽  
Relative Growth Rates ◽  
Yield Production ◽  
Northern Climate

Matching the growth pattern of a vegetable cultivar with the seasonal changes in climate is a prerequisite for successful yield production in a northern climate. This paper describes the growth characteristics of two carrot cultivars in relation to climatic conditions in two years, with special reference to the factors associated with high yield. Cv. Fontana produced twice as large a leaf area and shoot weight as cv. Panther. Increased partitioning to shoot in the former cultivar also resulted in a higher root yield. Uniformity in relative growth rates during the period of analysis suggests that intervarietal differences in the shoot to root ratio and in the yield potential appear very early. Nearly half of the root weight at final harvest was gained after mid-August, when temperature and daily irradiance began to decrease. A large leaf area may ensure better utilisation of diminishing growth resources at the end of the growing season. In the more favourable growing season, 1997, plants invested more in leaf production than they did in 1996: shoot fresh and dry weights were considerably higher but leaf area was not much higher.;

scholarly journals Stable and Oriented Liquid Crystal Droplets Stabilized by Imidazolium Ionic Liquids

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 6044
Author(s):  
Efthymia Ramou ◽  
Guilherme Rebordão ◽  
Susana I. C. J. Palma ◽  
Ana C. A. Roque
Keyword(s):  
Ionic Liquids ◽  
Liquid Crystal ◽  
Soft Matter ◽  
Sodium Dodecyl ◽  
High Yield ◽  
Molecular Features ◽  
Surface Active ◽  
State Of Matter ◽  
First Time ◽  
Sds Surfactant

Liquid crystals represent a fascinating intermediate state of matter, with dynamic yet organized molecular features and untapped opportunities in sensing. Several works report the use of liquid crystal droplets formed by microfluidics and stabilized by surfactants such as sodium dodecyl sulfate (SDS). In this work, we explore, for the first time, the potential of surface-active ionic liquids of the imidazolium family as surfactants to generate in high yield, stable and oriented liquid crystal droplets. Our results show that [C12MIM][Cl], in particular, yields stable, uniform and monodisperse droplets (diameter 74 ± 6 µm; PDI = 8%) with the liquid crystal in a radial configuration, even when compared with the standard SDS surfactant. These findings reveal an additional application for ionic liquids in the field of soft matter.

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