scholarly journals Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation

Metabolites ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 39 ◽  
Author(s):  
Daniel White ◽  
Paul Rooks ◽  
Susan Kimmance ◽  
Karen Tait ◽  
Mark Jones ◽  
...  
Keyword(s):  
Species Composition ◽  
Nutrient Limitation ◽  
Polar Lipid ◽  
Production Systems ◽  
Polar Lipids ◽  
Lipid Concentration ◽  
Chlorella Sp ◽  
Lipid Species ◽  
Liquid Chromatography Tandem Mass ◽  
Lipids Production

We evaluate the effects of nutrient limitation on cellular composition of polar lipid classes/species in Chlorella sp. using modern polar lipidomic profiling methods (liquid chromatography–tandem mass spectrometry; LC-MS/MS). Total polar lipid concentration was highest in nutrient-replete (HN) cultures with a significant reduction in monogalactosyldiacylglycerol (MGDG), phosphatidylglycerol (PG), phosphatidylcholine (PC), and phosphatidylethanolamine (PE) class concentrations for nutrient-deplete (LN) cultures. Moreover, reductions in the abundance of MGDG relative to total polar lipids versus an increase in the relative abundance of digalactosyldiacylglycerol (DGDG) were recorded in LN cultures. In HN cultures, polar lipid species composition remained relatively constant throughout culture with high degrees of unsaturation associated with acyl moieties. Conversely, in LN cultures lipid species composition shifted towards greater saturation of acyl moieties. Multivariate analyses revealed that changes in the abundance of a number of species contributed to the dissimilarity between LN and HN cultures but with dominant effects from certain species, e.g., reduction in MGDG 34:7 (18:3/16:4). Results demonstrate that Chlorella sp. significantly alters its polar lipidome in response to nutrient limitation, and this is discussed in terms of physiological significance and polar lipids production for applied microalgal production systems.

scholarly journals Total Fatty Acid and Polar Lipid Species Composition of Human Milk

Nutrients ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 158
Author(s):  
Talat Bashir Ahmed ◽  
Merete Eggesbø ◽  
Rachel Criswell ◽  
Olaf Uhl ◽  
Hans Demmelmair ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Species Composition ◽  
Human Milk ◽  
Polar Lipid ◽  
Milk Fat ◽  
Infant Health ◽  
Milk Samples ◽  
Lipid Species ◽  
The Relationship ◽  
Docosahexaenoic Acids

Human milk lipids are essential for infant health. However, little is known about the relationship between total milk fatty acid (FA) composition and polar lipid species composition. Therefore, we aimed to characterize the relationship between the FA and polar lipid species composition in human milk, with a focus on differences between milk with higher or lower milk fat content. From the Norwegian Human Milk Study (HUMIS, 2002–2009), a subset of 664 milk samples were analyzed for FA and polar lipid composition. Milk samples did not differ in major FA, phosphatidylcholine, or sphingomyelin species percentages between the highest and lowest quartiles of total FA concentration. However, milk in the highest FA quartile had a lower phospholipid-to-total-FA ratio and a lower sphingomyelin-to-phosphatidylcholine ratio than the lowest quartile. The only FAs associated with total phosphatidylcholine or sphingomyelin were behenic and tridecanoic acids, respectively. Milk FA and phosphatidylcholine and sphingomyelin species containing these FAs showed modest correlations. Associations of arachidonic and docosahexaenoic acids with percentages of phosphatidylcholine species carrying these FAs support the conclusion that the availability of these FAs limits the synthesis of phospholipid species containing them.

scholarly journals Polar Lipids Composition, Antioxidant and Anti-Inflammatory Activities of the Atlantic Red Seaweed Grateloupia turuturu

Marine Drugs ◽  
2021 ◽  
Vol 19 (8) ◽  
pp. 414
Author(s):  
Elisabete da Costa ◽  
Tânia Melo ◽  
Mariana Reis ◽  
Pedro Domingues ◽  
Ricardo Calado ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Polar Lipid ◽  
Polar Lipids ◽  
Bioactive Molecules ◽  
Integrative Approach ◽  
Red Seaweed ◽  
Grateloupia Turuturu ◽  
Lipid Species ◽  
Potential Applications ◽  
Anti Inflammatory

Grateloupia turuturu Yamada, 1941, is a red seaweed widely used for food in Japan and Korea which was recorded on the Atlantic Coast of Europe about twenty years ago. This seaweed presents eicosapentaenoic acid (EPA) and other polyunsaturated fatty acids (PUFAs) in its lipid fraction, a feature that sparked the interest on its potential applications. In seaweeds, PUFAs are mostly esterified to polar lipids, emerging as healthy phytochemicals. However, to date, these biomolecules are still unknown for G. turuturu. The present work aimed to identify the polar lipid profile of G. turuturu, using modern lipidomics approaches based on high performance liquid chromatography coupled to high resolution mass spectrometry (LC–MS) and gas chromatography coupled to mass spectrometry (GC–MS). The health benefits of polar lipids were identified by health lipid indices and the assessment of antioxidant and anti-inflammatory activities. The polar lipids profile identified from G. turuturu included 205 lipid species distributed over glycolipids, phospholipids, betaine lipids and phosphosphingolipids, which featured a high number of lipid species with EPA and PUFAs. The nutritional value of G. turuturu has been shown by its protein content, fatty acyl composition and health lipid indices, thus confirming G. turuturu as an alternative source of protein and lipids. Some of the lipid species assigned were associated to biological activity, as polar lipid extracts showed antioxidant activity evidenced by free radical scavenging potential for the 2,2′-azino-bis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS●+) radical (IC50 ca. 130.4 μg mL−1) and for the 2,2-diphenyl-1-picrylhydrazyl (DPPH●) radical (IC25 ca. 129.1 μg mL−1) and anti-inflammatory activity by inhibition of the COX-2 enzyme (IC50 ca. 33 µg mL−1). Both antioxidant and anti-inflammatory activities were detected using a low concentration of extracts. This integrative approach contributes to increase the knowledge of G. turuturu as a species capable of providing nutrients and bioactive molecules with potential applications in the nutraceutical, pharmaceutical and cosmeceutical industries.

scholarly journals A New Look for the Red Macroalga Palmaria palmata: A Seafood with Polar Lipids Rich in EPA and with Antioxidant Properties

Marine Drugs ◽  
2019 ◽  
Vol 17 (9) ◽  
pp. 533 ◽  
Author(s):  
Lopes ◽  
Melo ◽  
Meneses ◽  
Abreu ◽  
Pereira ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Eicosapentaenoic Acid ◽  
Lipid Content ◽  
Polar Lipid ◽  
Antioxidant Properties ◽  
Polar Lipids ◽  
Palmaria Palmata ◽  
Potential Health ◽  
Lipid Species ◽  
Red Macroalga

Palmaria palmata is an edible red macroalga widely used for human consumption and valued for its high protein value. Despite its low total lipid content, it is rich in eicosapentaenoic acid (EPA). This seaweed has been scarcely explored with regard to its lipid composition. The polar lipids of seaweeds are nowadays recognized as important phytochemicals contributing to their add value valorization and providing support for claims of potential health benefits. The present study aimed to disclose the polar lipid profile of P. palmata, farmed in an integrated multi-trophic aquaculture (IMTA) through modern lipidomic approaches using high-resolution LC-MS and MS/MS and to screen for the antioxidant properties of this red macroalga. A total of 143 molecular species of lipids were identified, belonging to several classes of polar lipids, such as glycolipids, phospholipids, and betaine lipids. It is noteworthy that the most abundant lipid species in each class were esterified with eicosapentaenoic acid (EPA), accounting for more than 50% of the lipid content. The polar lipid extract rich in EPA showed antioxidant activity with an inhibition concentration (IC) of IC30 = 171 ± 19.8 µg/mL for α,α-diphenyl-β-picrylhydrazyl radical (DPPH●) and IC50 = 26.2 ± 0.1 µg/mL for 2,20-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical cation (ABTS●+). Overall, this study highlights that P. palmata farmed in an IMTA framework can be a sustainable source of beneficial lipids with antioxidant activity. Moreover, this red macroalga can be exploited for future applications as a source of lipids rich in EPA for food and feed, nutraceuticals, and cosmetics.

Comparative study of tissue deposition of omega-3 fatty acids from polar-lipid rich oil of the microalgae Nannochloropsis oculata with krill oil in rats

2015 ◽  
Vol 6 (1) ◽  
pp. 185-191 ◽  
Author(s):  
Michael L. Kagan ◽  
Aharon Levy ◽  
Alicia Leikin-Frenkel
Keyword(s):  
Fatty Acids ◽  
Comparative Study ◽  
Polar Lipid ◽  
Rat Plasma ◽  
Nannochloropsis Oculata ◽  
Polar Lipids ◽  
Omega 3 Fatty Acids ◽  
Krill Oil ◽  
Omega 3 ◽  
Epa And Dha

An oil from micro-algae rich in EPA with no DHA and consisting of 15% polar lipids (phospholipids and glycolipids) showed equivalent uptake of EPA into rat plasma and organs as omega-3 krill oil consisting of EPA and DHA and 40% phospholipids.

scholarly journals Microalgae Cultivation in Different pH, Temperature and Media for Lipid Production

2014 ◽  
Vol 8 (2) ◽  
pp. 13-17 ◽  
Author(s):  
Dakshayini Jayaramareddy ◽  
Ravikumar Krishnappa ◽  
Girisha Sirangala Thimmappa
Keyword(s):  
Lipid Production ◽  
Life Sciences ◽  
Nutrient Supply ◽  
Polar Lipids ◽  
Microalgae Cultivation ◽  
Microalgal Biomass ◽  
Chlorella Sp ◽  
Nonpolar Lipids

Lipids produced by microalgal biomass can be grouped into nonpolar lipids and polar lipids, which can be easily converted into biofuels. Microalgal samples were collected from three different ponds of Bangalore and cultured in the laboratory to find the effect of different pH, temperature and media on the production of biomass and lipids. Among these, pH-9, temperature -25°C and Beneck’s media was most suitable for production of biomass (35.80 g/L) and lipids from the isolated microalgae Chlorella sp. compare to Chladospora sp. (13.33 g/L). Chlorella sp. Showed 0.32 (OD) at pH-9, 0.43 (OD) at temperature-25°C and 2.94 (OD) in Beneck’s media. Our result revealed that nutrient supply along with measured variables affects the production of biomass and lipids in different microalgae.DOI: http://dx.doi.org/10.3126/ijls.v8i2.10227 International Journal of Life Sciences Vol.8(2): 2014; 13-17

scholarly journals Ericoid mycorrhizal diversity increases with soil age and progressive phosphorus limitation across a 4.1 million-year chronosequence

2020 ◽  
Author(s):  
Devin R. Leopold ◽  
Kabir G. Peay ◽  
Peter M. Vitousek ◽  
Tadashi Fukami
Keyword(s):  
Species Composition ◽  
Nutrient Limitation ◽  
Mycorrhizal Fungi ◽  
Fungal Diversity ◽  
Species Turnover ◽  
Phosphorus Limitation ◽  
Fungal Species ◽  
Direct Role ◽  
Ericoid Mycorrhizal Fungi ◽  
Soil Age

AbstractEricaceous plants rely on ericoid mycorrhizal fungi for nutrient acquisition. However, the factors that affect the composition and structure of these fungal communities remain largely unknown. Here, we use a 4.1-myr soil chronosequence in Hawaii to test the hypothesis that changes in nutrient availability with soil age determine the diversity and species composition of fungi associated with ericoid roots. We sampled roots of a native Hawaiian plant, Vaccinium calycinum, and used DNA metabarcoding to quantify changes in fungal diversity and species composition. We also used a fertilization experiment at the youngest and oldest sites to assess the importance of nutrient limitation. We found an increase in diversity and a clear pattern of species turnover across the chronosequence, driven largely by putative ericoid mycorrhizal fungi. Fertilization with nitrogen at the youngest site and phosphorus at the oldest site reduced total fungal diversity, suggesting a direct role of nutrient limitation. Our results also reveal the presence of novel fungal species associated with Hawaiian Ericaceae and suggest a greater importance of phosphorus availability for communities of ericoid mycorrhizal fungi than is generally assumed.

scholarly journals Total, Neutral, and Polar Lipids of Brewing Ingredients, By-Products and Beer: Evaluation of Antithrombotic Activities

Foods ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 171 ◽  
Author(s):  
Ronan Lordan ◽  
Eoin O’Keeffe ◽  
Alexandros Tsoupras ◽  
Ioannis Zabetakis
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Platelet Aggregation ◽  
Acid Composition ◽  
Polar Lipid ◽  
Neutral Lipids ◽  
Fatty Acid Content ◽  
Polar Lipids ◽  
Lipid Fatty Acid ◽  
By Products

The in vitro antithrombotic properties of polar lipid constituents of malted grain (MG), pelleted hops (PH), brewer’s spent grain (BSG), spent hops (SH), wort, and bottled beer from the same production line were assessed in human platelets. The total lipids (TL) were extracted according to the Bligh and Dyer method and further separated into the total neutral lipids (TNL) and total polar lipids (TPL) extracts by counter-current distribution. The TL, TNL, and TPL extracts of all samples were assessed for their ability to inhibit platelet-activating factor (PAF) and thrombin-induced human platelet aggregation. The raw materials, by-products, wort, and beer lipid extracts all exhibited antithrombotic properties against PAF and thrombin. However, the beer TPL exhibited the lowest IC50 values against PAF-induced (7.8 ± 3.9 µg) and thrombin-induced (4.3 ± 3.0 µg) platelet aggregation indicating that these polar lipids were the most antithrombotic. The lipid extracts tended to be more bioactive against the thrombin pathway. The fatty acid content of all the TPL extracts were assessed using GC-MS. The fatty acid composition of the most bioactive TPL extracts, the wort and the beer, shared similar fatty acid profiles. Indeed, it was noted that fermentation seems to play a role in increasing the antithrombotic properties of polar lipids against PAF and thrombin by moderately altering the polar lipid fatty acid composition. Furthermore, the use of brewing by-products as a source of functional cardioprotective lipids warrants further investigation and valorisation.

scholarly journals Milk Polar Lipids: Underappreciated Lipids with Emerging Health Benefits

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1001 ◽  
Author(s):  
Liya Anto ◽  
Sarah Wen Warykas ◽  
Moises Torres-Gonzalez ◽  
Christopher N. Blesso
Keyword(s):  
Polar Lipid ◽  
Milk Fat ◽  
Dairy Products ◽  
Health Benefits ◽  
Total Lipid Content ◽  
Dietary Lipids ◽  
Polar Lipids ◽  
Food Sources ◽  
Gut Health ◽  
Milk Fat Globule

Milk fat is encased in a polar lipid-containing tri-layer milk fat globule membrane (MFGM), composed of phospholipids (PLs) and sphingolipids (SLs). Milk PLs and SLs comprise about 1% of total milk lipids. The surfactant properties of PLs are important for dairy products; however, dairy products vary considerably in their polar lipid to total lipid content due to the existence of dairy foods with different fat content. Recent basic science and clinical research examining food sources and health effects of milk polar lipids suggest they may beneficially influence dysfunctional lipid metabolism, gut dysbiosis, inflammation, cardiovascular disease, gut health, and neurodevelopment. However, more research is warranted in clinical studies to confirm these effects in humans. Overall, there are a number of potential effects of consuming milk polar lipids, and they should be considered as food matrix factors that may directly confer health benefits and/or impact effects of other dietary lipids, with implications for full-fat vs. reduced-fat dairy.

scholarly journals Dietary Polar Lipids and Cognitive Development: A Narrative Review

2019 ◽  
Vol 10 (6) ◽  
pp. 1163-1176 ◽  
Author(s):  
Lu Zheng ◽  
Mathilde Fleith ◽  
Francesca Giuffrida ◽  
Barry V O'Neill ◽  
Nora Schneider
Keyword(s):  
Cognitive Development ◽  
Human Milk ◽  
Polar Lipid ◽  
Critical Role ◽  
Daily Intake ◽  
Polar Lipids ◽  
Positive Effects ◽  
Lactating Mothers ◽  
Wide Range ◽  
The Brain

ABSTRACTPolar lipids are amphiphilic lipids with a hydrophilic head and a hydrophobic tail. Polar lipids mainly include phospholipids and sphingolipids. They are structural components of neural tissues, with the peak rate of accretion overlapping with neurodevelopmental milestones. The critical role of polar lipids in cognitive development is thought to be mediated through the regulation of signal transduction, myelination, and synaptic plasticity. Animal products (egg, meat, and dairy) are the major dietary sources of polar lipids for children and adults, whereas human milk and infant formula provide polar lipids to infants. Due to the differences observed in both concentration and proportion of polar lipids in human milk, the estimated daily intake in infants encompasses a wide range. In addition, health authorities define neither intake recommendations nor guidelines for polar lipid intake. However, adequate intake is defined for 2 nutrients that are elements of these polar lipids, namely choline and DHA. To date, limited studies exist on the brain bioavailability of dietary polar lipids via either placental transfer or the blood–brain barrier. Nevertheless, due to their role in pre- and postnatal development of the brain, there is a growing interest for the use of gangliosides, which are sphingolipids, as a dietary supplement for pregnant/lactating mothers or infants. In line with this, supplementing gangliosides and phospholipids in wild-type animals and healthy infants does suggest some positive effects on cognitive performance. Whether there is indeed added benefit of supplementing polar lipids in pregnant/lactating mothers or infants requires more clinical research. In this article, we report findings of a review of the state-of-the-art evidence on polar lipid supplementation and cognitive development. Dietary sources, recommended intake, and brain bioavailability of polar lipids are also discussed.

Grazing animals as habitat engineers for ground-nesting birds – a review of the issues

2005 ◽  
Vol 2005 ◽  
pp. 68-68
Author(s):  
K. Norris
Keyword(s):  
Species Composition ◽  
Vegetation Structure ◽  
Production Systems ◽  
Bird Species ◽  
Plant Species Composition ◽  
Integral Role ◽  
Avian Biodiversity ◽  
The Uk ◽  
Grassland Habitats ◽  
Ground Nesting

Habitats associated with livestock production systems are important in terms of avian biodiversity in the UK and across Europe. Livestock play an integral role in structuring grassland habitats, which in turn affects their suitability for different ground-nesting bird species. Such habitat engineering is driven mainly by the effect of grazing on plant species composition and vegetation structure. However, ground-nesting birds are also negatively affected by the presence of grazing animals since livestock destroy nests mainly through trampling.

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