scholarly journals Inhibition study of proinflammatory factor with ethanolic extract of propolis on innate immunity from diabetes mellitus mice model

2018 ◽  
Vol 90 (2) ◽  
Author(s):  
Bambang Pristiwanto ◽  
Sutiman B. Sumitro ◽  
Muhammad S. Djati ◽  
Aris Soewondo ◽  
Hideo Tsuboi ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Innate Immunity ◽  
Body Weight ◽  
Metabolic Diseases ◽  
Ethanolic Extract ◽  
Control Group ◽  
Mice Model ◽  
Innate Immunity Cells ◽  
Proinflammatory Factor ◽  
Ethanolic Extract Of Propolis

Health becomes an important topic today. One current problem was how to treat the effects of metabolic diseases, such as diabetes mellitus (DM). Thus, this study used an ethanolic extract of propolis (EEP), to test their ability as the supplement in the diabetes treatment to reduce inflammation, through proinflammatory factor response, especially nuclear factor κB (NF-κB). The streptozotocin- induced diabetes mellitus (SID) mice model was used, and expression of an proinflammatory factor was analyzed in their innate immunity cells with 3 doses of EEP, i.e. 50 mg/kg body weight, 100 mg/kg body weight, and 200 mg/kg body weight. Treatment of EEP in SID with three doses treatment decrease the number of macrophages with NF-κB expression significantly with DM control group. The results of B cells with NF-κB expression showed that EEP treatment in SID could decrease in dose 1 and dose 3, but not in dose 2. Proinflammatory cytokines expression of macrophage, especially Tumor Necrosis Factor-α and Interferon-γ, with EEP treatment in SID could decrease in three doses. This study suggests that EEP could reduce inflammation by inhibiting the development of NF-κB in innate immunity cells.

Antidiabetic Effects of Ethanolic Extract of Ficus glomerata (L.) Roots

2020 ◽  
Vol 16 (1) ◽  
pp. 33-41
Author(s):  
Mohini C. Upadhye ◽  
Uday Deokate ◽  
Rohini Pujari ◽  
Vishnu Thakare
Keyword(s):  
Body Weight ◽  
Glucose Tolerance ◽  
Density Lipoprotein ◽  
Ethanolic Extract ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Oral Glucose ◽  
Ficus Glomerata

Background: Ficus glomerata (F. glomerata) Linn. Family Moraceace is a large tree found all over India including outer Himalayan ranges, Punjab, Chota Nagpur, Bihar, Orissa, West Bengal, Rajasthan, Deccan and also as a common plant in South India. It is planted around the home and temples. It is cultivated throughout the year, distributed in evergreen forests and moist localities. Objective: The Ethanolic Extract of roots of F. Glomerata (EEFG) belonging to the family Moraceace, was investigated for its antidiabetic activity using alloxan induced diabetic rats. Methods: Thirty rats were divided into 5 groups having 6 rats in each group. The alloxan was administered to the rats of all groups except normal control group through intraperitoneal route at a concentration of 140mg/kg body weight. A dose of 100mg/kg and 200 mg/kg body weight of EEFG was administered to alloxan induced diabetic rats. The administration of the extract was lasted for 11 days. Effectiveness of the extract on glucose, cholesterol, triglycerides, and high density lipoprotein and protein concentrations was analyzed. Results: Significant (p<0.05) reduction in the levels of glucose, cholesterol, triglyceride of the diabetic rats was observed after treatment with ethanolic extract. After subjecting to oral glucose tolerance test EEFG also showed significant improvement in glucose tolerance. Conclusion: F. glomerata root ethanolic extract showed that it possesses antidiabetic effect and can be found useful for the management of diabetes mellitus.

scholarly journals Evaluation of Effectiveness of a Toothpaste Containing Tea Tree Oil and Ethanolic Extract of Propolis on the Improvement of Oral Health in Patients Using Removable Partial Dentures

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4071
Author(s):  
Karolina Wiatrak ◽  
Tadeusz Morawiec ◽  
Rafał Rój ◽  
Patryk Kownacki ◽  
Aleksandra Nitecka-Buchta ◽  
...  
Keyword(s):  
Oral Health ◽  
Oral Hygiene ◽  
Ethanolic Extract ◽  
Plaque Index ◽  
Control Group ◽  
Oral Microbiota ◽  
Study Group ◽  
Tea Tree Oil ◽  
Tea Tree ◽  
Ethanolic Extract Of Propolis

The aim of this study was to evaluate the effect of toothpaste containing natural tea tree essential oil (TTO) and ethanolic extract of propolis (EEP), on microflora and selected indicators of oral health in patients using removable acrylic partial dentures. Fifty patients with varying conditions of hygiene were divided into two groups. The study group received the toothpaste with TTO and EEP, while the control group received the same toothpaste but without TTO and EEP. At the first visit, oral hygiene and hygiene of the prostheses were carried out. Control visits took place 7 and 28 days later and compared to baseline. Indexes like API (Approximal Plaque Index), mSBI (modified Sulcus Bleeding Index), OHI-s (simplified Oral Hygiene Index), and DPI (Denture Plaque Index) were assessed in three subsequent stages, and swabs were collected from floor of the mouth area to assess the microbiota. After 7 and 28 days of using the toothpaste with TTO and EEP, a statistically significant decrease of the examined indicator values were observed in the study group as compared to the values upon the initial visit. The number of isolated strains of microorganisms in the study group was decreased or maintained at the same level, whereas in the control group an increase in the number of isolated strains was observed. The observed stabilization of oral microbiota in patients from the study group confirms the beneficial activity of toothpaste containing EEP and TTO compared to the control group.

scholarly journals Reproductive Characteristics, Serum Metabolites, and Oxidative Status in Female Guinea Pigs (Cavia porcellus) Fed with Ethanolic Extract of Dichrostachys glomerata Fruit

2021 ◽  
Vol 11 (1) ◽  
pp. 66-72
Author(s):  
Nadège Motchewo Djuissi ◽  
Ferdinand Ngoula ◽  
Justin Kouamo ◽  
Narcisse Bertin Vemo ◽  
Mathieu Fambo Stive Nono ◽  
...  
Keyword(s):  
Body Weight ◽  
Guinea Pigs ◽  
Ethanolic Extract ◽  
Oxidative Status ◽  
The Body ◽  
Control Group ◽  
Cavia Porcellus ◽  
Crown Rump Length ◽  
Serum Metabolites ◽  
Reproductive Characteristics

Dichrostachys glomerata (D. glomerata) is an aromatic plant which is used as a spice in cooking and Cameroonian traditional medicine to treat infertility in men. This work was designed to highlight the effects of the ethanolic extract of D. glomerata on oxidative status, serum metabolites and reproductive characteristics in female guinea pigs (Cavia porcellus). A total of 48 primiparous female guinea pigs, aged 4 months old with the body weight of 400 ± 10 g, were divided into four groups with two replications per group (6 guinea pigs each). During 90 days of trial, Group 1 (control group) orally received 1 ml/kg b.w. of distilled water daily, and groups 2, 3, and 4 received D. glomerata ethanolic extract once a day at doses of 50, 100, and 200 mg/kg b.w. using the same method of administration, respectively, for 90 days, including 60 days of gestation. After the first 30 days of treatment, mating was done by placing one non-treated male into cages containing six treated females. At the end of the treatment, data were collected on reproductive characteristics, serum metabolites, and oxidative stress markers. The results revealed that the ethanolic extract of D. glomerata induced a significant decrease in the number of post-implantation resorption and ovaries weight. Groups 3 and 4 showed a significant increase in the number of fetuses per dam and viable fetuses as well as placenta weight, compared to the control group. The serum level of progesterone significantly decreased in the group treated with 200 mg/kg D. Glomerata, compared to the other treated groups. The extract at 100 mg/kg body weight showed a significant increase in fetuses weight and fetuses crown-rump length, compared to the control group. Catalase activity significantly increased in the control group than D. glomerata treated groups. In conclusion, ethanolic extract of D. glomerata minimized reproductive stress and subsequently improved the reproductive performance of guinea pigs.

scholarly journals BLOOD GLUCOSE LEVELS AND THE MICROSCOPIC STRUCTURE OF KIDNEY WISTAR RAT DIABETES MELLITUS UNDER THE EFFECT OF LAWSONIA INERMIS (LINN.) LEAVES ETHANOLIC EXTRACT

2018 ◽  
Vol 11 (4) ◽  
pp. 257
Author(s):  
Mutiara Indah Sari ◽  
Maya Anjelir Antika ◽  
Dwi Rita Anggraini
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Wistar Rat ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Lawsonia Inermis ◽  
Control Group Design ◽  
Glucose Levels

 Objective: Lawsonia inermis (Linn.) leaves are one of the alternative medicines to treat diabetes mellitus in Indonesia. We investigated the blood glucose level (BGL) of the L. inermis (Linn.) leaves ethanolic extract (LLEE) leaves and evaluated the histopathological alterations in diabetic rats.Methods: This study was an experimental study with posttest - only control group design. Alloxan (120 mg/kg, intraperitoneally)-induced diabetic rats. 35 of Wistar rats (Rattus norvegicus) were divided randomly into five groups, i.e. K: Normal control, P1: Diabetic control, and P2, P3, and P4 (200 mg/kg body weight [BW], 400 mg/kg BW, and 600 mg/kg BW of LLEE, orally) for 28 days. At the end of the treatment, the rats were sacrificed to obtain the kidney for histopathological evaluation using hematoxylin and eosin technique. BGLs were conducted using a glucose meter (GlucoDR).Results: One-way ANOVA test showed that dose 400 mg/kg BW of the LLEE was related to BGL of alloxan-induced diabetic rats (p=0.000). The histopathological of kidney showed glomerular inflammation (GI), epithelial membrane lining degeneration, vascular congestion, and interstitial tubule hemorrhage at diabetic control (P1). Meanwhile, treated with 600 mg/kg BW of LLEE (P4) showed increase cellular regeneration as normal architecture of the kidney.Conclusion: The LLEE at dose 400 mg/kg BW effective decreased BGL and was able to restore the kidney destruction of alloxan-induced diabetic rats at dose 600 mg/kg BW.

scholarly journals HEPATOPROTECTIVE ACTIVITY OF VERNONIA AMYGDALINA LEAF ETHANOLIC EXTRACT IN WHITE RATS INDUCED BY PARACETAMOL

2018 ◽  
Vol 11 (10) ◽  
pp. 562
Author(s):  
Panal Sitorus ◽  
Nerdy Nerdy
Keyword(s):  
Body Weight ◽  
Methyl Cellulose ◽  
Ethanolic Extract ◽  
Scientific Data ◽  
Control Group ◽  
Carboxy Methyl Cellulose ◽  
Vernonia Amygdalina ◽  
White Rats ◽  
Significant Difference ◽  
Carboxy Methyl

Objectives: A study on the hepatoprotective effect of Vernonia amygdalina leaves ethanolic extract on white rats induced by paracetamol was aimed to obtaining scientific data and evidence the hepatoprotective ability of the extract.Methods: The research was a complete randomized design, using 36 white rats which were divided into 6 groups. The normal control group was given 1% carboxy methyl cellulose for 7 days and followed by distilled water 8 hours after treatment. The positive control group was given 100 mg curcumin per kg body weight of rats for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. The negative control group was given 1% carboxy methyl cellulose for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. The test group was given 50, 100 and 200 mg extract per kg body weight of rats for 7 days and followed by 2.5 g paracetamol per kg body weight after treatment. Blood sampling was carried out 3 times (before treatment, after treatment and after induction).Results: With doses of 50, 100, and 200 mg extract per kg body weight of rats give significant difference on the decrease of Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) levels after paracetamol administration.Conclusions: It can be concluded that Vernonia amygdalina leaves ethanolic extract were potentially hepatoprotective.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND EVALUATION OF ANTI-DIARRHOEAL ACTIVITY OF ETHANOLIC EXTRACT OF LEAVES OF CLERODENDRUM INFORTUNATUM

2017 ◽  
Vol 9 (4) ◽  
pp. 143
Author(s):  
Animesh Hazarika ◽  
Dipankar Saha
Keyword(s):  
Body Weight ◽  
Castor Oil ◽  
Herbal Remedy ◽  
Ethanolic Extract ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Weight Group ◽  
Group I ◽  
Antidiarrheal Activity

Objective: To determine preliminary phytochemical Screening and evaluation of Antidiarrheal activity of ethanolic extract of Leaves Clerodendrum infortunatum.Methods: The ethanolic extract of leaves of Clerodendrum infortunatum was prepared and analyzed for phytochemical constituents using standard method and evaluate scientifically the anti-diarrheal effect using leaves of Clerodendrum infortunatum by castor oil-inducing method. Animals were divided into four groups. Group-I was treated with distilled water 2 ml/kg body weight. Group-II was treated with loperamide 1 mg/kg body weight, Group-III and Group-IV were treated with ethanolic extract of leaves Clerodendrum infortunatum 200 mg/kg and 500 mg/kg body weight respectively.Results: Various phytochemical analysis revealed the presence of sterols, terpenoids, alkaloids, carbohydrates, tannins, glycoside, saponins, proteins and amino acids. The ethanolic extract of Clerodendrum infortunatum leaves given by oral route to mice at doses of 200 mg/kg and 500 mg/kg significantly showed antidiarrheal activity against castor oil induced diarrhea as compared with control group and standard.Conclusion: The present study concluded that ethanolic extract of leaves of Clerodendrum infortunatum contain the high presence of phytochemical components and it showed that the leaves of Clerodendrum infortunatum having significant antidiarrheal activity. All doses of the plant extracts showed a significant delay in castor oil-induced diarrhea and this justifies the use of this plant as a herbal remedy against diarrhea. 

scholarly journals Evaluation of Acute and Sub-Acute Dermal Toxicity Studies of Ethanolic Leaf Extract of Lawsonia Inermis in Rats

2016 ◽  
Vol 6 (1) ◽  
pp. 835-847 ◽  
Author(s):  
Ali Khairullah Zahi ◽  
Hazilawati Hamzah ◽  
Mohd Rosly Shaari ◽  
Riyanto Teguh Widodo ◽  
Lucy Johnny ◽  
...  
Keyword(s):  
Body Weight ◽  
Ethanolic Extract ◽  
Acute Experiment ◽  
Morphological Alteration ◽  
Control Group ◽  
Dermal Toxicity ◽  
Lawsonia Inermis ◽  
Ethanolic Leaf Extract ◽  
Observation Period

Lawsonia inermis is one of the most significant plants used in traditional medicine. However, many details of the dermal toxicity of L. inermis remain unknown. The objective of this study is to evaluate the in vivo acute and sub-acute dermal toxicity of ethanolic extract of L. inermis leaves. In acute experiment, a total of 20 rats were divided into four groups of five rats. A total of 30 rats were divided into five groups of six rats for the sub-acute experiment. The extract at a single dose of 2000 and 5000 mg/kg of body weight did not produce treatment-related signs of toxicity or mortality in all rats tested during the 14-day observation period. However, in a repeated dose 28-day study, the application of 500, 1000 and 2000 mg/kg of body weight/day of leaves extract revealed no significant change (p > 0.05) in bodyweight, haematological and biochemical parameters compared with the control group. Similarly, gross pathology and histopathology examinations of liver, kidneys, and skin did not reveal any morphological alteration. Overall, the results show that the close application of L. inermis leaves extract did not have any critically dangerous impact on rats. Subsequently, the concentrate may be employed for pharmaceutical plans.

scholarly journals Anti-diabetic activity of rhizome extracts of Imperata cylindrical against alloxan-induced Diabetes Mellitus in rats

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2704-2709
Author(s):  
Ranjana Kohli ◽  
Madan L Kaushik ◽  
Jai Parkash Kadian ◽  
Bhupendra Chauhan
Keyword(s):  
Body Weight ◽  
Glucose Level ◽  
Aqueous Extract ◽  
Ethanolic Extract ◽  
Treated Group ◽  
The Body ◽  
Control Group ◽  
Appreciable Effect ◽  
Aqueous Extracts ◽  
Study Results

The anti-diabetic effect of ethanolic and aqueous extracts of Imperata cylindrical  rhizomes was investigated in alloxan-induced diabeties in rats. Diabetes was induced by a single 150 mg/kg intraperitoneal dose of alloxan. Rats were divided into five groups with six rats in each group i.e. the normal control group, diabetic control group, standard group (glibenclamide, 10mg/kg, p.o.), Test-I group (200 mg/kg ethanolic extract) and Test-II group (200 mg/kg aqueous extract). The above concerned groups were inoculated on 21st day. On the last day of the experiment, fasted rats were killed by cervical dislocation. The body weight was measured at the initial day and final day. The blood samples were collected for estimation of glucose. The loss of body weight in control group, but recovery was observed in drug treated group. The serum glucose level was significant increased in diabetic rats. However, significant improvement was observed in treated group. The biochemical parameters such as HDL and proteins level were decreased in the control group but maintained in drug treated group. LDL, cholesterol, triglyceride creatinine and urea were significant increase in control group however, reduced level in drug treated group. The present study concluded that ethanolic and aqueous extracts of I. cylindrical  rhizome showed an appreciable effect in reducing the hyperglycemia and the complications associated with diabetes. However, aqueous extract is found more significant in decreasing blood glucose level in comparison to the ethanolic extract. The study results justify the traditional use of the plant as anti-diabetic.

scholarly journals Ameliorative Effect of Ethanolic Extract of Petroselinum Crispum Against Gentamicin-Induced Nephrotoxicity in Rabbits Male

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Sarah Hussain ◽  
Mariam Kadhem
Keyword(s):  
Body Weight ◽  
Clinical Signs ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Positive Control Group ◽  
Control Group ◽  
Petroselinum Crispum

The experiment was conducted to investigate the protective effect of Petroselinum crispum leave extracted against gentamicin-induced nephrotoxicity in male rabbits by studying the body weight, clinical signs, haematological and biochemical parameters, gross lesion and histopathological changes. Twenty four rabbits male were used and divided into 4 groups. Group 1: rabbits served as a negative control, received distilled water 1 ml(orally). Group 2: rabbits served as a positive control group, received gentamicin at a dose of 80 mg/kg/day intramuscular for 15 days. Group 3: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 125 mg/kg orally for 15 days. Group 4: rabbits received gentamicin at a dose of 80 mg/kg/day then after one hour treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg orally for 15 days.The results of the gentamicin treated group( positive control group) showed clinical signs such as loss of body weight, loss of appetite and rough hair with hematuria. The body weight a significantly declined (p≤ 0.05) compared other groups. There was a significant decrease (p≤ 0.05) in WBC count, lymphocyte, GSH, SOD, CAT, and GPX levels, while it recorded a significant increase (p≤0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA. Histological studies showed several kidney pathological changes such as pale colour, enlargement in size and weight and easy from detaching as opposed to negative control group. On the other hand, the group treated with ethanolic extractof Petroselinum crispum at dose 125 mg/kg induced improved of parameters as recorded significant increased(P ≤ 0.05) in body weight, WBC count, lymphocyte, GSH, SOD, CAT, and GPX, while significant decreased (P ≤ 0.05) in weights of the kidneys, neutrophils, creatinine, urea, and MDA compared with the positive control group whereas rabbits treated with ethanolic extract of Petroselinum crispum at dose 250 mg/kg restored the parameters and histological changes of the kidney to near normal status compared with the negative control group. These results showed that the dose-detected Petroselinum crispum extract (250mg / kg) acts as potential curative effect against gentamicin-induced nephrotoxicity in male rabbits.

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