scholarly journals Occurrence, Distribution and Antibiotic Sensitivity Pattern of Bacteria Isolated from Some Selected Fishes Sold in Bodija Market, Ibadan, Nigeria

2019 ◽  
pp. 1-7
Author(s):  
O. B. Balogun ◽  
O. Ogunsanmi ◽  
I. O. Olutimehin
Keyword(s):  
Antibiotic Sensitivity ◽  
Microbiological Analysis ◽  
Zone Of Inhibition ◽  
Salmonella Spp ◽  
Prochilodus Lineatus ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Fish Samples ◽  
Shigella Spp ◽  
Ibadan Nigeria

Fish constitutes the cheapest source of animal protein in the world. The nutritional qualities and the shelf ability of fish can be affected due to microbial contamination after harvesting. Hence, this study is designed to investigate the microbiological analysis of fish and the antibiotic sensitivity pattern of the isolated bacteria from some selected fish sold in Bodija Market in Ibadan, Nigeria. Three different fish samples: Catfish (Clarias gariepinus), Sabalo fish (Prochilodus lineatus) and Tadpole fish (Raniceps raninus) were bought from Bodija market in an ice packed bag and then transported to Microbiology laboratory for further analyses. Microbial analysis were carried out using serial dilution and pour plate methods. The antibiotic sensitivity pattern on the bacteria isolates were carried out using commercial antibiotics. E. coli, Pseudomonas aureginosa, Staphylococcus aureus, Serratia spp, Klebsiella spp, Salmonella spp, Shigella spp were isolated. Gram negative bacteria were more susceptible to most of the antibiotics used than gram positive bacteria. E. coli showed the highest susceptibility to all the antibiotics with varied zone of inhibitions. Salmonella spp show the highest susceptibility to septrin with the lowest zone of inhibition to pefloxacin. Shigella spp had the lowest zone of inhibition to gentamycin, rocephin, septrin and erythromycin when compared to other bacterial isolates. Serratia spp exhibited highest zone of inhibition to gentamycin. Fish contamination can be averted by employing good environmental conditions and preservation methods to avoid spoilage. The antibiotics sensitivity showed that all the bacteria isolated were more susceptible to antibiotics, thereby developing a resistant strain.

scholarly journals SEROTYPING AND ANTIBIOTIC SENSITIVITY TESTING OF ENTEROBACTERIAL PATHOGENS IN DIARRHOEAL PATIENTS OF KATHMANDU

2003 ◽  
Vol 41 (143) ◽  
pp. 392-6
Author(s):  
Abhignya Subedi ◽  
C D Shrestha ◽  
R P Adhikari
Keyword(s):  
Public Health ◽  
Shigella Flexneri ◽  
Antibiotic Sensitivity ◽  
Shigella Dysenteriae ◽  
Salmonella Spp ◽  
Sensitivity Testing ◽  
E Coli ◽  
The Family ◽  
National Public Health ◽  
Shigella Spp

In a study carried out at National Public Health Laboratory, Teku, 236 samples werecollected from the diarrhoeal patients and half of them were The patients below 15years of age. Altogether 61 pathogens belonging to the family Enterobacteriaceaewere isolated. The presence of pathogens in the patterns below 5 years was highlysignificant (P<0.05%). E coli was most common (68.8%) followed by Salmonella spp.(16.4%) and Shigella spp. (14.7%). Enteropathogenic was E. coli was the most frequentisolate (71.4%) among the pathogenic E. coli. Similarly, Salmonella typhimuriumwas the commonest salmonellae and Shigella dysenteriae and Shigella flexneri werethe most commonly isolated shigellae. Most of the pathogens were sensitive toCiprofloxacin (72.1%) and least was sensitive to Ampicillin (9.8%). Only 4.9% of thepathogens were sensitive to all the antibiotics tested.

Identification of pathogenic bacteria from food handling surfaces (tabletops) from different areas with demonstration of their drug resistance properties

2021 ◽  
Author(s):  
Anik Paul ◽  
Md. Mahmud Rahma ◽  
Tasnia Ahmed
Keyword(s):  
Fast Food ◽  
Pathogenic Bacteria ◽  
Klebsiella Oxytoca ◽  
Food Contamination ◽  
Antibiotic Sensitivity ◽  
Klebsiella Pneumonia ◽  
Microbiological Analysis ◽  
Food Handling ◽  
Salmonella Spp ◽  
Esherichia Coli

Foodborne illness is generally caused after consumption of food contaminated with pathogenic microorganisms. Food contamination often caused by contact with tabletops or food handling surfaces where the pathogenic microbes are present due to unhygienic condition of people working there and the overall environment of the food serving area. In current study, four areas (local restaurants, fast food shops, university canteens and hospital canteens) were selected for collection of swab sample (per cm2 area) from the tabletops. Five samples from each area were taken for further studies. After microbiological analysis we found ten different types of bacteria (Esherichia coli, Klebsiella pneumonia, Klebsiella oxytoca, Corynebacterium xerosis, Staphylococcus aures, Salmonella spp., Proteus mirabilis, Enterobacter aerogenes, pseudomonas aeruginosa and Alcaligenes fecalis) which are already considered to be pathogenic bacteria causing different health issues in immune-compromised and also in healthy consumers. These bacteria were then subjected to antibiotic sensitivity test using ten antibiotics-Vancomycin (30 µg), Cotrimoxazol (30 µg), Azithromycin (15 µg), Gentamicin (10 µg), Amoxycillin (10 µg), Cephradine (30 µg), Ceftriaxone (30 µg), Cefuroxime (30 µg), Cefoxitin (30 µg) and Tetracycline (30 µg). Bacterial isolates collected from university and hospital canteens showed most resistance towards these antibiotics. Strict maintenance of proper sanitation and hygiene starting from personal aspects to the overall environment of food handling service should be maintained to reduce the food contamination and foodborne disease.

scholarly journals Characterization and Antibiogram of Escherichia coli Associated with Mortality in Broilers and Ducklings in Bangladesh

1970 ◽  
Vol 2 (1) ◽  
pp. 9-14 ◽  
Author(s):  
MT Islam ◽  
MA Islam ◽  
MA Samad ◽  
SML Kabir
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Sensitivity ◽  
High Rate ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Highly Sensitive ◽  
Biochemical Studies ◽  
Increasing Trend ◽  
Escherichia Coli Infection ◽  
Hemorrhagic Enteritis

Mortality in broilers (6.56%) and growing ducks (11.0%) caused by Escherichia coli was recorded in the experimental flocks study during the period from May to August 2003. E. coli organisms isolated from broiler birds affected with characteristic lesions of omphalitis and yolk sac infection, fibrinous pericarditis and peri-hepatitis , hemorrhagic enteritis, and accumulation of excessive pericardial and peritoneal fluid, whereas from ducks with lesions of hemorrhagic enteritis and extensive epicardial hemorrhages. Each of the 21 isolates collected from broilers and 11 isolates from ducks was characterized by cultural and biochemical studies, of which 8 isolates from broilers and 5 isolates from ducks were tested for antibiotic sensitivity with 9 different antibiotics. The antibiotic sensitivity pattern showed that the isolates were highly sensitive to ciprofloxacin but an increasing trend of resistance was recorded in broilers (7 / 9) than duck (4 / 9) isolates. It may be concluded from the results of this study that the high rate of E. coli infection in broilers and ducks along with the high resistance of isolates to antibiotics constitute a threat to the poultry industry in Bangladesh. Key words: Escherichia coli infection; mortality; broilers; ducks; characterization; antibiogram doi: 10.3329/bjvm.v2i1.1927 Bangl. J. Vet. Med. (2004). 2 (1) : 09-14

scholarly journals Bacterial Etiology and Antibiotic Sensitivity Pattern of Community Acquired Pneumonia in Diabetic Patients: Experience in a Tertiary Care Hospital in Bangladesh

2017 ◽  
Vol 7 (2) ◽  
pp. 101-105
Author(s):  
Jamal Uddin Ahmed ◽  
Mohammad Delwar Hossain ◽  
Muhammad Abdur Rahim ◽  
Farhana Afroz ◽  
AKM Musa
Keyword(s):  
Staphylococcus Aureus ◽  
Klebsiella Pneumoniae ◽  
Antibiotic Sensitivity ◽  
Community Acquired Pneumonia ◽  
Sputum Culture ◽  
Diabetic Patients ◽  
Care Hospital ◽  
E Coli ◽  
Sensitivity Pattern ◽  
Pseudomonas Species

Background: Diabetes mellitus (DM) is an immunosuppressive condition and uncontrolled diabetes is associated with increased susceptibility to various infections like pneumonia. Community acquired pneumonia (CAP) in diabetic patients is often caused by more virulent or atypical organisms and associated with increased resistance to conventional antibiotics. The aims of this study were to identify the bacterial etiology of CAP in patients with DM and to see their antibiotic sensitivity pattern.Methods: This was a cross-sectional, observational study conducted in the Department of Internal Medicine & Pulmonology of BIRDEM General Hospital, Dhaka, Bangladesh, from January 2013 to December 2015. A total of 120 hospitalized diabetic patients diagnosed with CAP and with a positive sputum culture growth of any bacteria were included in the study.Results: Majority (67%) of the patients were male. Mean age of the patients was - 55.69 ±10.5 years. Mean duration of diabetes was - 7.35 ±1.3 years. Mean HbA1c was - 8.6 ±1.89%. Sputum for culture showed that out of 120 (100%) patients, Klebsiella pneumoniae was detected in 53 (44.2%) patients, Staphylococcus aureus in 18 (15.0%), Pseudomonas species in 16 (13.3%) patients, Acinetobacter in 10 (8.3%), Escherichia coli in 9 (7.5%) patients and 14 (11.7%) patients had growth of other organisms. Sensitivity pattern of different bacterial growth in sputum to commonly used antibiotics like ceftriaxone, ciprofloxacin, amikacin and imipenem were as follows – Klebsiella (19%, 47%, 74%, 96% respectively), Staph aureus (11%, 33%, 78%, 67% respectively), Pseudomonas (19%, 75%, 81%, 88% respectively), Acinetobacter (0%, 0%, 20%, 50% respectively), E. coli (22%, 22%, 100%, 100% respectively). All (100%) of the Pseudomonas and Acinetobacter were sensitive to colistin. Most of the growth of all the bacteria (Klebsiella 94%, Staphylococcus 78%, Pseudomonas 81%, Acinetobacter 100%, E. coli 100%) occurred in patients with poor glycemic control (HbA1c ? 7.0%).Conclusion: This study results suggest that CAP in diabetic patients are more frequently due to Klebsiella pneumoniae, Staphylococcus aureus and Pseudomonas species and mostly they are less sensitive to commonly used antibiotics like ceftriaxone and ciprofloxacin. So, whenever possible, treatment of CAP should be guided by sputum culture and sensitivity test and for empirical treatment of CAP in diabetic patients, alternative antibiotics like imipenem and amikacin should be considered.Birdem Med J 2017; 7(2): 101-105

scholarly journals ISOLATION AND IDENTIFICATION OF MICROFLORA FROM APPARENTLY HEALTHY CAGED PARROTS OF DHAKA ZOO OF BANGLADESH

1970 ◽  
Vol 8 (1) ◽  
pp. 05-10 ◽  
Author(s):  
J Akhter ◽  
MT Hossain ◽  
MT Islam ◽  
MP Siddique ◽  
MA Islam
Keyword(s):  
Research Work ◽  
Antibiotic Sensitivity ◽  
Biochemical Properties ◽  
Gram Negative Bacteria ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Gram Negative ◽  
E Coli ◽  
Apparently Healthy

The research work was conducted to isolate and identify the microflora from apparently healthy caged parrots. A total of 45 samples (oral swabs, cloacal swabs and feces) were collected from five types of caged parrots (Gray cockatiels, Rose ringed parakeet, Alexandriane parakeet, Red breast parakeet and Blossom headed parakeet) of Dhaka Zoo during the period from April to August 2009. The samples were cultured on different bacteriological media and the bacteria were identified by their cultural and biochemical properties. All the isolates were allowed for antibiogram study. The bacteria isolated in this study from different types of caged parrots were E. coli (64.44%), Salmonella spp. (46.67%), Staphylococcus spp. (46.67%), Pasteurella spp. (33.33%), Proteus spp. (6.67%) and some unidentified Gram-positive and Gram-negative bacteria. Of these isolates, E. coli was the most frequent isolate. The frequency of Gram-negative bacteria was higher in this study. The percentage of bacterial isolates recovered from each type of parrots was almost similar. Irrespective of types of parrots, the higher percentage of different bacteria was isolated from cloacal swab (77.78%) followed by feces (75.56%). The 68.89% isolates were recovered from oral swab. All the suspected isolates of Salmonella spp. were confirmed by slide agglutination test using Salmonella polyvalent ‘O’ antiserum. Among the 21 Salmonella spp. isolated in this study, 4 (19.05%) isolates were identified as S. Pullorum when tested with specific antisera against S. Pullorum. The results of antibiotic sensitivity tests revealed that ampicillin and amoxicillin were completely resistant to E. coli and Pasteurella spp.; ampicillin to Proteus spp.; and furazolidone to Salmonella spp. and Pasteurella spp. However, the antibiotics of fluoroquinolone group such as ciprofloxacin, norfloxacin and enrofloxacin showed moderate to high sensitivity against almost all the bacterial isolates. Of these, ciprofloxacin was found to be consistently highly sensitive to all the bacterial isolates. DOI = 10.3329/bjvm.v8i1.8349 Bangl. J. Vet. Med. (2010). 8(1): 05-10

scholarly journals Isolation Of Extended-Spectrum B-Lactamase (ESBL) Producing Escherichia coli and Klebsiella pneumiae From Dr. Zainoel Abidin General Hospital, Aceh

2019 ◽  
Vol 4 (1) ◽  
pp. 16-22 ◽  
Author(s):  
Zinatul Hayati ◽  
Syamsul Rizal ◽  
Ridhia Putri
Keyword(s):  
Escherichia Coli ◽  
General Hospital ◽  
Clinical Specimen ◽  
Antibiotic Sensitivity ◽  
High Sensitivity ◽  
Clinical Microbiology Laboratory ◽  
Isolation And Identification ◽  
E Coli ◽  
Extended Spectrum ◽  
Sensitivity Pattern

Infection that occurs in Indonesia has increased more significantly than before, compared to the increasing bacterial multidrug resistance (MDR) as the cause of infection. A study conducted in 5 hospitals in Indonesia in 2013 showed that the prevalence rate of extended-spectrum β-lactamase (ESBL)-producing bacteria reached 32-68%. The objective of this study is to detect the prevalence and resistence pattern of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Dr. Zainoel Abidin General Hospital, Banda Aceh. This study was conducted from 1 September 2016 to 31 December 2016. Specimen types included in this study were blood, sputum, urine, pus, mucosal swab, and another body fluids sample. The sampling method in this study was total sampling that is all clinical specimen examined in Clinical Microbiology Laboratory. Isolation and identification ESBL-producing bacteria was performed by VITEK-2 machine (Biomerieux). The result of this study is that a total 122 E. coli and K. pneumoniae were isolated. That consisted of 48 (39%) E. coli isolates and 74 (61%) K. pneumoniae isolates. From 48 E. coli isolates it was found out that 41 (85%) had ESBL phenotypes and from 74 K. pneumoniae isolates it was found out that 59 (80%) had ESBL phenotypes. In total, 100 (82%) isolates from 122 isolates had ESBL phenotypes. Distribution of ESBL-producing E. coli and K. pneumoniae based on sample was 24 (89%) isolates from the total of 27 urine isolates, 18 (95%) isolates from the total of 19 blood isolates, 28 (78%) isolates from the total of 36 sputum isolates, and 30 (75%) isolates from the total of 40 pus isolates. Antibiotic sensitivity pattern of the E. coli and K. pneumoniae isolates had high sensitivity to amycasin dan meropenem which was above 89%. Meanwhile, it also had sensitivity to Fosfomycin and Piperacyclin-Tazobactam by 80% and 77% respectively. Another antibiotic was less effective

scholarly journals Microbiological analysis of different categories of food items in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1351-1356
Author(s):  
M.A. Uddin ◽  
T. Jabin ◽  
E.A. Siam ◽  
M.T. Afreen ◽  
S.S. Uddin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Wide Range ◽  
Shigella Spp

Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, bloodstream infection etc. The current study was attempted to observe the microbiological quality of some popular foods collected from different places of Dhaka city, Bangladesh. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. Higher counts of TVBC, coliform, Staphylococcus spp. and fungal load were recorded as 1.46×107 CFU/g (yogurt 1), 4.5×106 CFU/g (yogurt 1), 5.6×105 CFU/g (raw meat) and 2.9×103 CFU/g (sea fish), respectively. Fecal coliform was detected only in one out of ten samples. On the contrary Salmonella spp., Vibrio spp., Shigella spp. and Pseudomonas spp. were completely absent. The antibiogram study showed that all the isolates were sensitive against Kanamycin and Azithromycin. Better sensitivity was also observed against Gentamycin and Ciprofloxacin. Varying degree of antibiotic resistance was also detected against Cefixime, Amikacin and Neomycin. Our study emphasizes the need for continuous monitoring of the various categories of food samples for the safety of public health.

scholarly journals Occurrence of pathogenic microorganisms in dessert items collected from Dhaka city

2020 ◽  
Vol 9 (1) ◽  
pp. 19-22
Author(s):  
Shohana Akter ◽  
Anasua Sarkar ◽  
Kamal Kanta Das
Keyword(s):  
Heterotrophic Bacteria ◽  
Good Health ◽  
Poor Quality ◽  
Dhaka City ◽  
Salmonella Spp ◽  
E Coli ◽  
Shigella Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Due to delicious taste and readily availability, desserts are one of the most popular foods in Dhaka city. High amount of carbohydrate and protein in dessert items make them more susceptible to proliferation of microbial growth. Present study depicted a complete microbiological profile of some popular desserts such as, sweet, pastry, ice cream, pudding, falooda, yogurt and custard available in different food shop at Dhaka city, Bangladesh. All the samples were found to be contaminated with heterotrophic bacteria as well as fungi within the range of 103 to 106 cfu/g. In case of specific microflora, the growth of Staphylococcus spp., Klebsiella spp. and Pseudomonas spp. were observed in most of the samples indicates the poor quality of these products. Bioburdens of E. coli in sweet, pudding and yogurt were found in the range of 1.2×102 to 2.7×103 cfu/g. Salmonella spp., Shigella spp. and Vibrio spp. could not be isolated from any of the samples. Current study indicates that hygienic conditions should be maintained during preparation, packaging and retailing of dessert items in order to reduce the load of contamination in ready to eat foods which will ensure good health of consumers. Stamford Journal of Microbiology, Vol.9(1) 2019: 19-22

scholarly journals Antibiotic sensitivity pattern of bacterial isolates from urine samples of admitted patients with urinary tract infection in a tertiary care teaching hospital of Tripura, India: a hospital record based study

2018 ◽  
Vol 7 (4) ◽  
pp. 585
Author(s):  
Uttam K. Das ◽  
Prithul Bhattacharjee ◽  
Shubhaleena Debnath ◽  
Maitrayee Chakraborty ◽  
Ranjib Ghosh ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Antibiotic Resistance ◽  
Urinary Tract ◽  
Tract Infection ◽  
Teaching Hospital ◽  
Antibiotic Sensitivity ◽  
Urine Samples ◽  
Hospital Record ◽  
E Coli ◽  
Sensitivity Pattern

Background: Urinary tract infection (UTI) being one of the most common and a serious health problem both in the community and hospital settings each year worldwide, the emergence of antibiotic resistance in the management of UTI is a serious public health issue. The present study will analyse the antimicrobial sensitivity pattern of pathogens isolated from the urine samples of admitted patients suffering from UTI in Tripura Medical College and Dr. B.R. Ambedkar Memorial Teaching Hospital (TMC).Methods: This was a hospital record-based study. The urine samples of clinically diagnosed UTI patients admitted in various departments of the hospital during the study period were included. The reports of culture and sensitivity testing of the samples were collected. The results were interpreted according to the guidelines of the Clinical and Laboratory Standards Institute (CLSI).Results: During the 12-month study period, a total of 752 urine samples were analysed. Enterococcus (43.75%) was the most frequently isolated bacteria, followed by E. coli (28.45%) and Klebsiella (14.89%). Enterococcus was highly sensitive (p<0.001) to vancomycin (95.33%), E. coli was mostly sensitive to nitrofurantoin (83.65%) and Klebsiella mainly sensitive to imipenem (75.49%).Conclusions: The study showed that positive urine culture with the antibiotic sensitivity of the isolates is very important for antimicrobial therapy, as antibiotic resistance is a worldwide problem which causes ineffectiveness of treatment.

Sign in / Sign up

Export Citation Format

Share Document