scholarly journals Transcriptomic Analysis of Camellia Sinensis (L.) Kuntze Var. Niaowangensis Q. H. Chen Leaves Under Cold Stress

2021 ◽  
Author(s):  
Ying Wang ◽  
Cheng Wan ◽  
Leijia Li ◽  
Zhun Xiang ◽  
Jihong Wang ◽  
...  
Keyword(s):  
Cold Stress ◽  
Candidate Genes ◽  
Cold Tolerance ◽  
Camellia Sinensis ◽  
Resistance Breeding ◽  
Guizhou Province ◽  
Cdna Libraries ◽  
Control Group ◽  
P Value ◽  
Illumina Hiseq

Abstract Fine varieties of the Yunwu Tribute Tea (Camellia Sinensis (L.) Kuntze var. niaowangensis Q. H. Chen) are distributed on the Yunwu Mountain, Guiding County, Guizhou province, China. Cold stress usually occurs in winter and is one of the most significant environmental factors restricting the growth of this plant as well as its geographical distribution. However, only few systematic studies have examined the molecular mechanism of cold tolerance in the Yunwu Tribute Tea. Hence, in this study, Illumina HiSeq technology was applied to investigate the cold-tolerance mechanism and for this purpose, cDNA libraries were obtained from two groups of samples namely, the cold-treated group (DW) and the control group (CK). A total of 185,973 unigenes were produced from 511,987 assembled transcripts and among these, 16,020 differentially expressed genes (DEGs) (corrected p-value <0.01, |log2(fold change)| >3), including 9,606 upregulated and 6,414 downregulated genes, were obtained. Moreover, the antioxidant enzyme system, plant hormone signal transduction, proline metabolism, tyrosine metabolism pathway, and transcription factors were analyzed and based on the results, a series of candidate genes related to cold stress were screened out and discussed. The physiological indexes related to the low temperature response were tested, along with five DEGs which were validated by quantitative real-time PCR. For this study, it is expected that the results of the transcriptome sequence of Yunwu Tribute Tea will provide valuable clues for genetic studies while helping to screen candidate genes for cold-resistance breeding in tea plants.

scholarly journals Integrated mRNA and Small RNA Sequencing for Analyzing Leaf Spot Pathogen Didymella segeticola and Its Host, Tea (Camellia sinensis), During Infection

2020 ◽  
pp. MPMI-07-20-0207
Author(s):  
Rui Yang ◽  
Silong Jiang ◽  
Dongxue Li ◽  
Qiaoxiu Yin ◽  
Xian Wu ◽  
...  
Keyword(s):  
Camellia Sinensis ◽  
Leaf Spot ◽  
Resistance Mechanisms ◽  
Guizhou Province ◽  
Future Research ◽  
Small Rna Sequencing ◽  
Illumina Hiseq ◽  
Tea Plants ◽  
Open Access Article

Leaf spot on tea plants (Camellia sinensis [L.] Kuntze), caused by the fungus Didymella segeticola (Q. Chen) Q. Chen, Crous & L. Cai (syn. Phoma segeticola), negatively affects the productivity and quality of tea leaves in Guizhou Province, China. Although the genome sequence of D. segeticola has been published, no data on the transcriptome or microRNAs (miRNAs) of the pathogen or host during infection are available. Here, we report on the high-quality transcriptome and miRNA sequences of both D. segeticola and tea during infection, using the Illumina HiSeq 4000 or HiSeq 2500 platforms. Comprehensive expression profiling of the fungal pathogen and its host will provide a resource for future research into trait-specific genes of the pathogen and the host as well as on host-pathogen interactions and on disease resistance mechanisms. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

scholarly journals Transcriptomic Analyses of Camellia oleifera ‘Huaxin’ Leaf Reveal Candidate Genes Related to Long-Term Cold Stress

2020 ◽  
Vol 21 (3) ◽  
pp. 846 ◽  
Author(s):  
Lingli Wu ◽  
Jian’an Li ◽  
Ze Li ◽  
Fanhang Zhang ◽  
Xiaofeng Tan
Keyword(s):  
Cold Stress ◽  
Candidate Genes ◽  
Cold Tolerance ◽  
Soluble Sugars ◽  
Chilling Tolerance ◽  
Hunan Province ◽  
Camellia Oleifera ◽  
Qrt Pcr ◽  
Hilly Region

‘Huaxin’ is a new high-yielding timber cultivar of Camellia oleifera of high economic value, and has been widely cultivated in the red soil hilly region of Hunan Province of the People´s Republic of China in recent years. However, its quality and production are severely affected by low temperatures during flowering. To find genes related to cold tolerance and further explore new candidategenes for chilling-tolerance, Illumina NGS (Next Generation Sequencing) technology was used to perform transcriptomic analyses of C. oleifera ‘Huaxin’ leaves under long-term cold stress. Nine cDNA libraries were sequenced, and 58.31 Gb high-quality clean reads were obtained with an average of 5.92 Gb reads for each sample. A total of 191,150 transcripts were obtained after assembly. Among them, 100,703 unigenes were generated, and 44,610 unigenes were annotated. In total, 1564 differentially expressed genes (DEGs) were identified both in the A_B and A_C gene sets. In the current study, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed, andrevealed a group of cold-responsive genes related to hormone regulation, photosynthesis, membrane systems, and osmoregulation; these genes encoded many key proteins in plant biological processes, such as serine/threonine-protein kinase (STPK), transcription factors (TFs), fatty acid desaturase (FAD), lipid-transfer proteins (LTPs), soluble sugars synthetases, and flavonoid biosynthetic enzymes. Some physiological indicators of C. oleifera ‘Huaxin’ were determined under three temperature conditions, and the results were consistent with the molecular sequencing. In addition, the expression levels of 12 DEGs were verified using quantitative real-time polymerase chain reaction (qRT-PCR). In summary, the results of DEGs analysis together with qRT-PCR tests contribute to the understanding of cold tolerance and further exploring new candidate genes for chilling-tolerance in molecular breeding programs of C. oleifera ‘Huaxin’.

scholarly journals Pengaruh Konsumsi Tanin Ekstrak Daun Teh Hitam (Camellia sinensis) Dosis Bertingkat Terhadap Prevelensi Kejadian Anemia Yang Di Tinjau Dari Level Hemoglobin Dan Hematokrit Pada Mus musculus bablc Albino

2019 ◽  
Vol 1 (1) ◽  
pp. 17-22
Author(s):  
Dini Qurrata Ayuni , Armait , Ratna Syafnir
Keyword(s):  
Camellia Sinensis ◽  
Mus Musculus ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
One Way Anova

Anemia merupakan salah satu masalah kesehatan di Indonesia dengan prevalensi 21,7% (3.068.328 jiwa), Anemia dapat terjadi oleh bebagai faktor seperti faktor nutrisi , salah satu nutrisi yang dapat mengakibatkan anemia adalah kebiasaan minum teh. Tujuan dari penelitian ini untuk mengetahui pengaruh konsumsi tanin ekstrak daun teh hitam (camellia sinensis) dosis bertingkat terhadap prevelensi kejadian anemia yang ditinj au dari level Hb dan hematokrit pada mus musculus bablc albino . Jenis penelitian ini adalah eksperimental dengan desain penelitian post test only control group design. Dengan sampel sebanyak 24 ekor mencit dibagi dalam empat kelompok dengan dosis 0,504 ml , 1,016ml , 1,5ml selama empat belas hari. Darah di ambil melalui ekor mencit, pemeriksaan hemoglobin menggunakan Hb Sahli dan pemeriksaan hematokrit menggunakan centrifuge. Penelitian ini dilakukan di labor anatomi fisiologi dan L2 Dikti. Hasil analisis dat a menggunakan one way anova dan Uji LSD dengan derajat kepercayaan 95%. Dari data diketahui terdapat perbedaan rata - rata kadar hemoglobin dan hematocrit tiap kelompok. Rata - rata hemoglobin pada tiap kelompok adalah 11,41, pada kelompok P1: 9,38, P2: 7,65 dan P3: 6,70, sedangkan pada hematocrit kelompok kontrol 53,17, kelompok perlakuan P1: 47,75, P2: 43,91 dan P3: 40,29. Hasil uji dengan menggunakan one way anova, didapatkan ada pengaruh pemberian teh hitam terhadap kadar hemoglobin (p value= 0,00) dan hematocrit (p value 0,017). Terdapatnya pengaruh konsumsi tanin ekstrak daun teh hitam (camellia sinensis) dosis bertingkat terhadap prevelensi kejadian anemia yang ditinjau dari level Hemoglobin dan hematokrit pada mus musculus bablc albino. Disarankan kep ada pekonsumsi teh agar lebih memperhatikan dosis teh hitam dengan batas normal 2.7gr/hari, sehingga aman dikonsumsi

ASSESSMENT OF CORRELATION BETWEEN LEUKODERMA AND PSYCHIATRIC ILLNESS

2019 ◽  
Vol 3 (8) ◽  
Author(s):  
Dr. Mukesh Batra
Keyword(s):  
Psychiatric Illness ◽  
Study Participant ◽  
Simple Random Sampling ◽  
Anxiety Symptoms ◽  
Control Group ◽  
P Value ◽  
Common Symptom ◽  
Cross Sectional ◽  
Duration Of Disease ◽  
Illness Anxiety

Background: Leukoderma is an acquired disease of pigmentation which is presented by depigmented areas of different shape and sizes on the skin. In various researches it was reported that these defects in melanocytes occurred due to autoimmune pathway or the complex causation of oxidative stress and genetics. Material & Methods: In the present cross‑sectional prospective study 100 patients who were diagnosed with Leukoderma and controls who were not having depigmentation of skin and without any known clinical disease were enrolled for present study by simple random sampling. Written informed consent was taken from each study participant. Clearance from institutional ethical committee was also taken prior to the study. Results: The prevalence of psychiatric illness among leukoderma group was 22% and among 78% patients we did not found any psychiatric illness. The most common symptom was depression which was present among 22% of patients with Leukoderma and 18% patients with Leukoderma had anxiety symptoms. Among the control group depression was present in 5 % subjects and anxiety symptoms were present in 7% of subjects. The mean WHO-Quality of life scores was low among Leukoderma group in relation to the control group (p value <0.05). On correlation with psychiatric illness, observations were statistically non-significant (p > 0.05) with HAMA-A and HAMA- D. The observations were statistically significant (p < 0.05) with GHQ total and BSA score. Conclusion:  Higher prevalence of psychitric morbidity among patients with leukoderma and it was found associated with duration of disease and BSA scores. The most common symptom was depression which was followed by anxiety symptoms. Key words: Leukoderma, psychiatric illness, anxiety, depression.

SUPPORTIVE EDUCATIVE SYSTEM DALAM MENINGKATKAN KEMANDIRIAN MERAWAT KAKI PADA PASIEN DIABETES MELLITUS TIPE II

2019 ◽  
Vol 9 (2) ◽  
pp. 63-72
Author(s):  
Nova Nurwinda Sari ◽  
Herlina Herlina
Keyword(s):  
Diabetes Mellitus ◽  
Health Center ◽  
Intervention Group ◽  
T Test ◽  
Control Group ◽  
P Value ◽  
Type Ii ◽  
Foot Care ◽  
Control Group Design ◽  
Patients With Diabetes

Diabetes mellitus dapat menyebabkan cukup banyak komplikasi seperti kelainan mata, kelainan ginjal, kelainan pembuluh darah dan kelainan pada kaki. Penderita diabetes mellitus yang mengalami komplikasi kronis perlu diberikan upaya preventif untuk mencegah komplikasi, salah satunya adalah kemampuan perawatan kaki. Penelitian ini dilakukan untuk menguji efektivitas supportive educative system dalam meningkatkan kemandirian perawatan kaki pada pasien dengan diabetes mellitus Tipe II di Puskesmas Permata Sukarame, Bandar Lampung. Penelitian ini menggunakan metode quasy eksperimen dengan desain pretest-posttest with control group dengan total masing-masing kelompok sebanyak 18 responden. Pengumpulan data dilakukan dengan menyebarkan kuesioner kepada responden yang memenuhi kriteria inklusi penelitian. Penelitian ini diuji menggunakan analisis univariat, bivariat dan uji T-test. Hasil penelitian menunjukkan bahwa terdapat perbedaan rata-rata dalam kemandirian perawatan kaki pada kelompok intervensi dan kelompok kontrol dengan p-value 0,000. Pendidikan dan praktik perawatan kaki harus diberikan sejak dini sebagai upaya pencegahan untuk komplikasi.   Kata kunci : Supportive educative system, kemandirian perawatan kaki   SUPPORTIVE EDUCATIVE SYSTEM IN IMPROVING INDEPENDENCE OF FOOT CARE IN PATIENTS WITH DIABETES MELLITUS TYPE II   ABSTRACT Diabetes mellitus can cause quite a lot of complications such as eye disorders, kidney disorders, vascular disorders and abnormalities in the legs. Patients with diabetes mellitus who have chronic complications need to be given a preventive effort to prevent complications, one of which is foot care ability. This research was conducted to examine the effectiveness of supportive educative systems in increasing the independence of foot care in patients with Type II diabetes mellitus in the Permata Sukarame Health Center Bandar Lampung Working Area. This study used a quasi-experimental method with pretest-posttest with control group design with a total of 18 respondents each. Data collection is done by distributing questionnaires to respondents who meet the research inclusion criteria. This study was tested using univariate, bivariate, T-Test analysis. The results showed that the mean differences in the independence of foot care in the intervention group and the control group in the Permata Sukarame Community Health Center work area with a p-value of 0,000. Education and practice of foot care should be given early as a preventative effort for complications.   Keywords: Supportive educative system, independence of foot care

NILAI ANKLE BRACHIAL INDEX PADA PENDERITA DIABETES MELITUS TIPE 2 SESUDAH MELAKUKAN BUERGER ALLEN EXERCISE DI PUSKESMAS WILAYAH KECAMATAN NGANJUK

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Supriyadi . ◽  
Nurul Makiyah ◽  
Novita Kurnia Sari
Keyword(s):  
Ankle Brachial Index ◽  
Control Group ◽  
P Value ◽  
Test Design ◽  
Purposive Sampling ◽  
Paired Samples ◽  
Post Test ◽  
Diabetes Melitus

<p><em>Buerger Allen Exercise</em> mampu meningkatkan pemakaian glukosa oleh otot yang aktif sehingga glukosa dalam darah dapat menurun, dapat membantu mencegah terjadinya penyakit arteri perifer, serta meningkatkan aliran darah ke arteri dan berefek positif pada metabolisme glukosa. Penelitian ini bertujuan untuk mengetahui nilai <em>ankle brachial index</em>pada penderita diabetes melitus tipe 2setelah melakukan <em>Buerger Allen exercise</em>. Jenis penelitian ini adalah <em>quasy-experiment </em>dengan<em> pre-post test design with control group</em><em>.</em> Jumlah sampel 60 penderita diabetes melitus tipe 2 dengan <em>purposive sampling</em>, dibagi menjadi 2 kelompok yaitu kelompok perlakuan dan kelompok kontrol. Responden kelompok perlakuan diberikan intervensi <em>Buerger Allen exercise</em> sebanyak 12 kali  selama 15 hari.Penelitian dilakukan di wilayah Puskesmas Kecamatan Nganjuk.Data hasilpengukuran nilai <em>ankle brachial index</em>berupa ratio dan diuji statistik dengan <em>Paired Samples Test</em>. Didapatkan <em>p value</em> 0.001 untuk kelompok perlakuan (<em>p value</em>&lt; 0.05) yang menunjukkan bahwa adanya perubahan bermakna secara statistik nilai <em>ankle brachial index</em> sesudah melakukan <em>Buerger Allen exercise</em>. Dapat disimpulkan bahwa nilai <em>ankle brachial index</em>pada penderita diabetes melitus tipe 2 meningkat sesudah melakukan <em>Buerger Allen exercise</em>.</p><p> </p><p> <strong>Kata kunci :penderita diabetes melitus tipe2, <em>Buerger Allen Exercise, Ankle brachial index</em></strong></p><p> </p>

Penatalaksanaan IMD pada Ibu Postpartum Sectio Caesarea Mempengaruhi Status Gizi dan Kecepatan Produksi ASI

2020 ◽  
Vol 2 (2) ◽  
pp. 112-120
Author(s):  
Nursari Abdul Syukur ◽  
Susi Purwanti
Keyword(s):  
Breast Milk ◽  
Nutritional Status ◽  
Milk Production ◽  
Quantitative Research ◽  
Milk Protein ◽  
Sectio Caesarea ◽  
Control Group ◽  
P Value ◽  
Study Results

Many mothers who give birth to Sectio Caesarea (SC) do not Initiate Early Breastfeeding (IMD), which fails exclusive breastfeeding. This study aimed to determine the effect of IMD management in postpartum SC mothers on nutritional status, speed of milk production, and quality of breast milk protein. Method: quantitative research with quasi approach experiment. The research design used was a pre-post-test control non-equivalent control group. A sampling of this study used the Consecutive method sampling with a sample of 20 mothers who gave birth by cesarean section (SC). Hypothesis testing uses the independent t-test and the Mann-Whitney test. The study results showed an influence on the management of IMD in postpartum SC mothers on the speed of ASI production (p-value=0.004) and nutritional status (p-value=0.028). There was no effect of IMD management on postpartum SC mothers on the quality of breast milk protein (p-value = 0.543). This study recommends that the hospital implement an IMD promotion program before the abdominal wall is closed as a form of intervention to increase milk production and maternal nutritional status

The Estimation of prostate specific antigen (PSA) concentrations in patients with prostatitis by fully automated ELISA technique.

2020 ◽  
Vol 3 (11) ◽  
pp. 1100-1104
Author(s):  
Hussein Naeem Aldhaheri ◽  
Ihsan Edan AlSaimary ◽  
Murtadha Mohammed ALMusafer
Keyword(s):  
Personal Information ◽  
Receptor Gene ◽  
Specific Antigen ◽  
Gene Clusters ◽  
Control Group ◽  
P Value ◽  
Group Data ◽  
Elisa Technique ◽  
And Control

      The Aim of this study was to determine Immunogenetic expression of  Toll-like receptor gene clusters related to prostatitis, to give acknowledge about Role of TLR in prostatitis immunity in men from Basrah and Maysan provinces. A case–control study included 135 confirmed prostatitis patients And 50 persons as a control group. Data about age, marital status, working, infertility, family history and personal information like (Infection, Allergy, Steroid therapy, Residency, Smoking, Alcohol Drinking, Blood group, Body max index (BMI) and the clinical finding for all patients of Prostatitis were collected. This study shows the effect of PSA level in patients with prostatitis and control group, with P-value <0.0001 therefore the study shows a positive significant between elevated PSA levels and Prostatitis.

PENINGKATAN KEMAMPUAN PEMAHAMAN DAN KREATIVITAS MATEMATIS SISWA DENGAN MENGGUNAKAN MODEL PENCAPAIAN KONSEP

2013 ◽  
Vol 19 (1) ◽  
pp. 52
Author(s):  
Adi Suarman Situmorang
Keyword(s):  
Random Sampling ◽  
Control Group ◽  
P Value ◽  
Design Data ◽  
Control Group Design ◽  
Group Design ◽  
Post Test ◽  
Test Control

Abstract Tujuan penelitian bertujuan untuk mengetahui peningkatan kemampuan kreativitas matematika siswa yang diajar dengan model pencapaian konsep lebih tinggi dari siswa yang diajar dengan pembelajaran konvensional. Mengetahui interaksi antara model pembelajaran pencapaian konsep dengan tingkat kemampuan matematika siswa terhadap peningkatan kemampuan kreativitas matematika siswa. Penelitian ini dilaksanakan di SMA Negeri 5 Medan dengan jumlah sampel sebanyak 80 siswa dari 364 siswa SMA kelas X melalui teknik random sampling, Penelitian ini merupakan suatu studi eksperimen dengan desain penelitian pre-test-post-test control group design. Data diperoleh melalui nilai semester untuk kemampuan awal matematis (KAM), tes kemampuan pemahaman matematis, tes kemampuan kreativitas matematis. Data dianalisis dengan uji ANAVA dua jalur. Hasil analisis data menunjukkan bahwa rata-rata tes kemampuan kreativitas eksperimen dan kontrol adalah 13,3 dan 7,58 dengan p-value (2-tailed) adalah 0, dengan 0 < α = 0,05 maka terdapat perbedaan kemampuan kreativitas matematik siswa yang diajarkan dengan Model Pencapaian Konsep (MPK) dan Pendekatan Pembelajaran Konvensional, nilai signifikan sebesar 0,732, karena 0,732 > 0,05 maka tidak ada interaksi antara pendekatan pembelajaran dengan kemampuan awal siswa terhadap perbedaan kemampuan kreativitas matematik siswa. .

PENGGUNAAN SELAPUT AMNION SEGAR PADA INSISI LUKA OPERASI SEKSIO SESAREA

2019 ◽  
Vol 1 (2) ◽  
pp. 47-55
Author(s):  
Syahredi Syaiful Adnani ◽  
Hafni Bachtiar
Keyword(s):  
Wound Healing ◽  
Caesarean Section ◽  
Amniotic Membrane ◽  
Healing Time ◽  
Cost Of Care ◽  
Control Group ◽  
P Value ◽  
Control Groups ◽  
And Control ◽  
Wound Healing Time

In the last few decades, the incidence of caesarean section is increasing in the world, especially in Indonesia. One of the way to treat tissue scar is through biologic and synthetic dressing where nowadays, amnion has been used as biologic dressing frequently. This study was conducted to determine the effect of the use of fresh amniotic membrane on wound incision Caesarean section compared with Caesarean section incision wound covered using regular gauze bandages and fixated with plaster in RS. Dr. Reksodiwiryo Padang. The design of this study is an experimen-tal study with Post test design with control group design. Sampling was done using a formula consecutive sampling two different test samples obtained an average of 72 people for each group. The analysis used include univariate and bivariate analyzes. The average wound healing time the difference was statistically significant (p value <0.05) in the treatment and control groups. There was highly significant difference in the proportion of local infection on day 3 between the treatment and control groups (p value <0.05). There were very significant differences in the proportion of local allergic reactions at day 3, and 5 between the treatment and control group (p <0.05). There are significant differences in terms of the cost of care per day between treatment and control groups (p <0.05). From this study, the average wound healing time has a very significant difference.Keywords: Fresh Amniotic Membrane, Wound Cesarean Section, Wound Healing

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