scholarly journals Synergistic action of Viteselen with anti-Fasciola drug as a tool for improving fertility and hemato-biochemical biomarkers  in Fasciola infected Sheep 

2021 ◽  
Author(s):  
Ghada H. Abdel-Rahman ◽  
Shimaa Abdel-Radi
Keyword(s):  
Hematological Parameters ◽  
Treated Group ◽  
Reproductive Hormones ◽  
Infected Sheep ◽  
Synergistic Action ◽  
Post Treatment ◽  
Economic Losses ◽  
Control Group ◽  
Complete Disappearance ◽  
Biochemical Biomarkers

Abstract Fascioliasis causes public health problems and economic losses all over the world. The present study aimed to evaluate the synergistic action between Viteselen (anti-oxidant) and specific anti-Fasciola drug (Triclalbendazole (TCBZ) for improvement body condition of F. gigantica naturally infected sheep with reference to some hematological and biochemical biomarkers in their sera. Animals were divided into five equal groups include G-1 as control non-infected animals, G-2 to G-5 are Fasciola naturally infected animals. G-2 is non-treated group, animals in G-3 were treated with TCBZ, animals in G-4 were injected by Viteselen and those in G-5 were treated by both TCBZ and Viteselen. The results revealed significant decrease in mean eggs in feces and F. gigantica circulating antigens (FCAg) in sera of TCBZ treated sheep after the 1st week post treatment. Complete disappearance of eggs from feces of drug treated groups was recorded at 21st d.p.t. While the value of FCAg decreased to negative at 14th d.p.t. The highest significant improvement (p < 0.05) in the estimated hematological parameters (RBCs, Hb and TLC), liver enzymes (AST and ALT), oxidative stress and antioxidant markers (TAC, MDA, SOD and GSH) and reproductive hormones (Progesterone and Estradiol) was recorded in animals in G-5 followed by G-3. While non-significant improvement was recorded in animals in G-4 in comparison with those in the control group. This improvement increased with increasing the time post treatment. For conclusion; using of Viteselen in association with specific anti-parasitic drug improved the general health parameters and reproductive performance of the investigated sheep.

scholarly journals EFFICACY OF IVERMECTIN AGAINST GASTROINTESTINAL NEMATODIASIS AND ECTOPARASITES IN CROSSBRED CATTLE IN BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 191-197
Author(s):  
M. Ahammed ◽  
M. A. Ali ◽  
M. A. Ehsan ◽  
M. Mostafa
Keyword(s):  
Body Weight ◽  
Treatment Period ◽  
Hematological Parameters ◽  
Research Work ◽  
Live Weight ◽  
Treated Group ◽  
Post Treatment ◽  
Control Group ◽  
Crossbred Cattle ◽  
Group A

The experiment was carried out to determine the efficacy of Ivermectin (Ivomec® S/C Formulation) against gastrointestinal nematodiasis and ectoparasites including stephanofilaria on crossbred cattle, to determine the effect of drug on certain hematological parameters like hemoglobin (Hb) content, packed cell volume (PCV) and erythrocyte sedimentation rate (ESR)in crossbred cattle and to determine the effect of the drug on live weight. A total of 100 cattle were selected randomly and examined for presence of both endo and ectoparasites including stephanofilaria. Gastrointestinal nematodiasis were detected by examination of fecal samples and ectoparasites as well as stephanofilaria were detected by physical examination. Out of 100 cattle, 20 were found to suffer from both endo and ectoparasites. These 20 cattle were selected finally for the research work. Then these cattle were divided into two groups, group A (treated group, n=15) and group B (control group, n=5). Ivermectin was injected subcutaneously to the cattle of group A @ 200µm/kg body weight (1ml/50 kg body weight). The therapeutic efficacy of the drug against gastrointestinal nematodiasis was determined by investigation of fecal egg count reduction and the efficacy was 100% against the common nematodes in crossbred cattle on day 7, 14, 21 and 28 of post treatment period. Ticks within a markable area (25 square inches) were counted on day 0 and lice infestation was marked as infected on day 0. Ivermectin showed 100% effectiveness at the 7, 14, 21 and 28 day of post treatment period against both tick and lice infestation. The efficacy of the drug against stephanofilariasis (humpsore) was determined by reduction of diameter of the sore on the day 14, 42 and 56 of post treatment period. All the sores were completely healed up by the 56 days. During the study of hematological parameters it was seen that Hb and PCV were increased whereas ESR values were decreased on post treatment days. In this study the mean live weight of the cattle of treated group was increased after treatment with ivermectin and increased body weight was 5.13% on the 28th day of post treatment period. From the above findings it may be concluded that ivermectin was 100% effective against gastrointestinal nematodes common in crossbred cattle. Effectiveness of the drug against external parasites was 100%. In this study hematological parameters were changed significantly after treatment with ivermectin, body weight of animal treated with ivermectin was increased and no adverse effect of the drug was found in this study.

scholarly journals Effect of ZnO nanoparticles in treatment of broilers with Eimeria tenella. experimentally infected.

2021 ◽  
Author(s):  
Sadiya Aziz Anah ◽  
Khadeeja Abees Hmood ◽  
Saad Aziz Anah
Keyword(s):  
Zinc Oxide ◽  
Severe Disease ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Eimeria Tenella ◽  
Positive Control Group ◽  
Economic Losses ◽  
Control Group ◽  
New Treatment

Abstract Coccidiosis is one of the common poultry diseases in Iraq and the world, which causes great economic losses. Therefore, this study aimed to use a new treatment represented by nanoparticles of zinc oxide and compare it with the drug (Amprolium). The Oocysts of Eimeria tenella were isolated from the feces of the briolers received at the Veterinary hospital in Diwaniyah Province and initially diagnosed by compound light microscopy,Then it was confirmed molecularly by PCR technique using the internal transcribed space ITS1 gene with a molecular weight of 409bp. The experiment was carried out on 120 birds of broilers, as the animals were divided into 6 groups with 20 birds per group. The first group(G1) was left as a negative control(uninfected), the second group(G2) was inoculated with 50,000 mature Oocysts and left as a positive control group,the third group(G3) was incubated with 50,000 Oocysts + Amprolium (anticoccidial), the fourth, fifth and sixth groups(G4,G5,G6) were also dosed with 50,000 Oocysts followed by an oral dose of nanoparticles of zinc oxide at a concentration of 20,40,60 mg/kg for each of fourth, fifth and sixth groups respectively. The current results showed that the briolers in G2 suffered from severe disease symptoms and high mortality occurred in the first week of infection, amounting to %10 compared to the anticoccidial (Amprolium group and the groups treated with nanoconcentrations 20,40,60 mg/kg respectively.G6 which was dosed with a concentration of 60 mg /kg, showed a significant decrease in the number of Oocysts excreted compared with the positive control group and the anticoccidial group, in addition to the appearance of mild symptoms and a mortality rate of %0.8,While there was a decrease in the number of Oocysts excreted during the second week in the anticoccidial group and the treated groups with the three nanoconcentrations with a decrease in the mortality rates of birds .The number of Oocysts excreted in the litter significantly decreased in the treated group with a concentration of 60 mg/kg compared to all other groups, and all birds of this group recovered during the second week of infection.

scholarly journals Ameliorative Effects of Fenugreek Seeds and Curcumin on Hematotoxicity induced by Nicotine in Male Albino Rats

2022 ◽  
Vol 3 (1) ◽  
pp. 01-08
Author(s):  
Azab Elsayed Azab ◽  
Mohamed Omar Albasha ◽  
Manal Abuelkasem Elnaif
Keyword(s):  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Group Iv ◽  
Control Group ◽  
Albino Rats ◽  
Group V ◽  
Fenugreek Seeds ◽  
Group Iii ◽  
Group I

The present study aimed to investigate the ameliorative effects of fenugreek seeds and curcumin on hematotoxicity induced by nicotine in male albino rats. 30 male F-344/NHsd Fischer rats, weighing from 180 to 200g were used in the present study. The animals were divided into five groups (6 rats for each); Group I (control group), Group II (nicotine treated group), Group III (nicotine/fenugreek seeds co-administered), Group IV (nicotine/curcumin co-administered), and Group V (nicotine/curcumin& fenugreek seeds co-administered). At the end of the experimentation and 24 hours after the last dose, all animals were anaesthetized with ether and blood samples were collected by heart puncture. The samples were collected in clean dry tubes containing the anticoagulant substance EDTA and used for the hematological studies. The results showed that the animals treated with nicotine for 4 weeks showed a significant decrease in RBCs count, hemoglobin concentration, hematocrit value, MCH, MCHC, and platelets count, and increased MCV and WBCs count as compared to the control group. Co-administration of nicotine with fenugreek and/or curcumin caused improvement in all hematological parameters when compared with nicotine group. It can be concluded that nicotine had a strong effect on the hematological parameters. The ingestion of fenugreek and/or curcumin prevent the hematoxicity induced by nicotine. The current study suggests that fenugreek and curcumin may be useful in combating free radical-induced hematotoxicity induced by nicotine.

scholarly journals Cellular Damage After Prolonged Low-Dose Exposure of Neonicotinoid in Rats

2021 ◽  
Author(s):  
Rahat Naseer ◽  
Affan Tariq ◽  
Munazza Raza Mirza ◽  
Muhammad Rashid ◽  
Syed Qasim Raza ◽  
...  
Keyword(s):  
Vitamin E ◽  
Low Dose ◽  
Prolonged Exposure ◽  
Cell Damage ◽  
Hematological Parameters ◽  
Treated Group ◽  
Alpha Tocopherol ◽  
Cellular Damage ◽  
Control Group ◽  
New Class

Abstract Background; Dinotefuran is a new class of neonicotinoids claimed to be harmless to mammals and humans. This claim was daunted by the documented effect of dinotefuran on honeybees and further studies were required. Aim: The study was designed to assess the capaciousness of damage caused by prolonged exposure of dinotefuran in mammals and probable strategy to neutralize its effect. Methodology: Ninety-day trial using Wistar rats (n=45) was conducted while dividing them into three groups: untreated control group, insecticide (dinotefuran) treated group, and dinotefuran treated and vitamin E supplemented group. Dinotefuran was administrated orally (LD25). Vitamin E (alpha-tocopherol) supplementation was given in water ad libitum. Blood sampling was done twice a month, and hematological and biochemical data were recorded. After expiry of trial period, the experimental rats were anesthetized and sacrificed. Organs (kidneys, liver, and heart) were isolated from each groups, weighed, and stored at approximately -20°C till further processing, analysis and histopathology were performed. Results: All the hematological parameters were affected significantly. Histopathology of tissues showed clear necrosis in all the tissues except kidneys. All the biomarkers of oxidative stress and comet assay demonstrated significant cell damage. All the parameters showed improvement after vitamin E supplementation but non-significantly. Significance: These findings were suggestive that even low dose persistent exposure can lead to mutagenicity and carcinogenicity in mammals and other non-target species hence revised policy guidelines and more intelligent use of these chemicals is required.

scholarly journals Effect of Methanolic Leaf Extract ofOcimum basilicumL. on Benzene-Induced Hematotoxicity in Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
S. Saha ◽  
M. K. Mukhopadhyay ◽  
P. D. Ghosh ◽  
D. Nath
Keyword(s):  
Cell Cycle ◽  
Leaf Extract ◽  
Bone Marrow Cells ◽  
Hematological Parameters ◽  
Treated Group ◽  
Protective Role ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Cell Cycle Deregulation

The aim of the present study was to investigate the protective role of methanolic leaf extract ofOcimum basilicumL. against benzene-induced hematotoxicity in Swiss albino mice. GC analysis and subacute toxicity level of the extract were tested. Mice were randomly divided into three groups among which II and III were exposed to benzene vapour at a dose 300 ppm × 6 hr/day × 5 days/week for 2 weeks and group I was control. Group III of this experiment was treated with the leaf methanolic extract at a dose of 100 mg/kg body weight, a dose in nontoxic range. Hematological parameters (Hb%, RBC and WBC counts), cell cycle regulatory proteins expression and DNA fragmentation analysis of bone marrow cells was performed. There was an upregulation of p53 and p21 and downregulation of levels of CDK2, CDK4, CDK6, and cyclins D1 and E in leaf extract-treated group. DNA was less fragmented in group III compared to group II (P<0.05). The present study indicates that the secondary metabolites ofO. basilicumL. methanolic leaf extract, comprising essential oil monoterpene geraniol and its oxidized form citral as major constituents, have modulatory effect in cell cycle deregulation and hematological abnormalities induced by benzene in mice.

scholarly journals Distribution of selenium in sheep treated with dipheny diselenide

2018 ◽  
Vol 70 (4) ◽  
pp. 1017-1022
Author(s):  
M.L.R. Leal ◽  
J.B.T. Rocha ◽  
C.L.D. Corte ◽  
A.R. Aires ◽  
J.F.X. Rocha ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Single Dose ◽  
Intravenous Injection ◽  
Selenium Concentration ◽  
Treated Group ◽  
Post Treatment ◽  
Control Group ◽  
Diphenyl Diselenide ◽  
Single Intravenous Injection

ABSTRACT The aim of the present study was to report the in vivo distribution of selenium in sheep. For this, animals were allocated into two groups (control group and treated group) and kept in metabolic cages for a period of 37 days. The treated group received a single dose (6µmol/kg) of Diphenyl Diselenide, intravenously. Plasma and erythrocytes samples were collected at different times. Adipose tissue, muscles (latissimusdorsi, semitendinosus, and supra-scapular) heart, liver, lung, kidney, intestine and brain were sampled at 30 days post-treatment, in order to determine the selenium concentration. The results demonstrated that the selenium, from the Diphenyl Diselenide group, was higher in erythrocytes (4.8mg/L, six hours post-treatment) when compared with the control sheep. The deposition of selenium occurred in the liver (7.01µg/g), brain (3.53µg/g) and kidney (2.02µg/g). After 30 days of a single intravenous injection of Diphenyl Diselenide, liver was the main organ of selenium deposition.

scholarly journals Effect of Resveratrol on Hematological and Biochemical Alterations in Rats Exposed to Fluoride

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Nurgül Atmaca ◽  
Ebru Yıldırım ◽  
Bayram Güner ◽  
Ruhi Kabakçı ◽  
Fatih Sultan Bilmen
Keyword(s):  
Enzyme Activity ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Treated Group ◽  
Inorganic Phosphorus ◽  
Control Group ◽  
Protective Effects ◽  
Biochemical Alterations ◽  
Fluoride Treatment ◽  
Hematological And Biochemical Parameters

We investigated the protective effects of resveratrol on hematological and biochemical changes induced by fluoride in rats. A total of 28 rats were divided into 4 groups: control, resveratrol, fluoride, and fluoride/resveratrol(n=7each), for a total of 21 days of treatment. Blood samples were taken and hematological and biochemical parameters were measured. Compared to the control group, the fluoride-treated group showed significant differences in several hematological parameters, including decreases in WBC, RBC, and PLT counts and neutrophil ratio. The group that received resveratrol alone showed a decrease in WBC count compared to the control group. Furthermore, in comparison to the control group, the fluoride group showed significantly increased ALT enzyme activity and decreased inorganic phosphorus level. The hematological and biochemical parameters in the fluoride + resveratrol treated group were similar to control group. In the fluoride + resveratrol group, resveratrol restored the changes observed following fluoride treatment, including decreased counts of WBC, RBC, and PLT, decreased neutrophil ratio and inorganic phosphorus levels, and elevated ALT enzyme activity. The present study showed that fluoride caused adverse effects in rats and that resveratrol reduced hematological and biochemical alterations produced by fluoride exposure.

scholarly journals Effects ofSalvadora persicaExtract on the Hematological and Biochemical Alterations against Immobilization-Induced Rats

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Kholoud S. Ramadan ◽  
Salha A. Alshamrani
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Hdl Cholesterol ◽  
Treated Group ◽  
Control Group ◽  
Serum Samples ◽  
Salvadora Persica ◽  
Biochemical Alterations ◽  
Hematological And Biochemical Parameters

A total of 24 rats were divided into 4 groups: control, stress, extract alone, and stress + extract (n=6each), for total 21 days of treatment. The immobilization stress was induced in rats by putting them in 20 cm × 7 cm plastic tubes for 2 h/day for 21 days. Rats were postorally treated withSalvadora persicaat a dose of 900 mg/kg body weight via intragastric intubations. At the end of the test period, hematological and biochemical parameters were determined in blood and serum samples with determination of vital organs weights. The vital organ weights were not significantly affected in stressed rats as compared to control rats. Compared to the control group, the stress treated group showed significances in several hematological parameters, including decreases in WBC, RBC, and PLT counts. Furthermore, in comparison to the control group, the stress group showed significantly increased blood glucose, serum total cholesterol, LDL-cholesterol, and triacylglycerols levels and decreased HDL-cholesterol level. The hematological and biochemical parameters in the stress + extract treated group were approximately similar to control group. The SP extract restored the changes observed following stress treatment.

scholarly journals The Modulatory Effects of Pentoxifylline in Biochemical Changes Induced By 17α-Ethinyl Estradiol in the Rat Model

2018 ◽  
Vol 12 (4) ◽  
pp. 5-9
Author(s):  
Aysen Kor ◽  
◽  
Ebrahim Shahroozian ◽  
Ahmadi-Hamedani Mahmood ◽  
Saeideh Naeimi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Normal Saline ◽  
Ethinyl Estradiol ◽  
Hematological Parameters ◽  
Serum Levels ◽  
Treated Group ◽  
Control Group ◽  
Acid Uptake ◽  
Duration Of Treatment ◽  
Tissue Samples

Background: Ethinylestradiol (EE) has induced cholestasis and hepatotoxicity in animal studies through reducing bile acid uptake by hepatocytes and induce of oxidative stress. Pentoxifylline (PTX) is a drug that by inhibition of release or transcription of proinflammatory cytokine cause prevents oxidative stress of liver cell and reduction of damage. We aimed to evaluate the effects of pentoxifylline on liver injury induced by Ethinylestradiol in rats. Methods: Twenty-four female Wistar rats (300±20 gr) were divided into four groups, equally. Duration of treatment was 5 consecutive days for each group. The control group Simultaneously received orally and subcutaneously normal saline. PTX group Simultaneously received Pentoxifylline orally and normal saline subcutaneously, EE Group Simultaneously received EE subcutaneously and normal saline orally. In the EE+PTX group, rats Simultaneously received EE subcutaneously and PTX orally. Rats were anesthetized and blood and tissue samples were collected for measurement of hematological and biochemical parameters. Results: The EE administration increased the serum levels of ALP and MDA significantly. The EE administration also decreased albumin and GPX levels were significant. These aberrations were improved by PTX treatment in EE + PTX group. Most of hematological parameters were not significant by the EE. The plasma level of TNF- in the PTX+ES treated group showed a significant decrease in comparison to that in the ethinyl estradiol group. Conclusion: PTX has partial capacity to protect against liver changes induced by Ethinyl Estradiol.

scholarly journals Effect of ketorolac administration post bone cavitation surgery on some hematological parameters

2017 ◽  
Vol 41 (1) ◽  
pp. 169-177
Author(s):  
Raffal A. Omar
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Post Operation

     This study was designed to determine the effect of ketorolac on some hematological parameters post cavitation surgery in domestic rabbits. Ninety-six adult domestic local breed male rabbits were used in this study, weighing (1.23 ± 0.33) kg. They were divided randomly into two groups in the first one (n= 48/group) which represented control group, while the second one (n=48/group) represented the treated group (Ketorolac group). All rabbits in both groups undergoes surgical operation (bone cavitation in femur bone) under general anesthesia. In the Ketorolac group, animals were treated by using 30 mg/kg of Ketorolac directly post-operation and continued daily for 5 days, while in Control group no treatment was given postoperatively. The hematological parameters were recorded which include white blood cells count; red blood cells count; hemoglobin; packed cell volume; mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration; mean corpuscular volume and platelets at the period of (3, 5, 7, 14, 21, 28, 35, and 42) days post operation were estimated. Results concerning hematological values showed no significant differences in packed cell volume values between control and treated group and within each group, while haemoglobin values showed a significant decrease at period 7 and 14 days between control and treated group. Within control group the significant decrease were noticed clearly at period of 7 days. Data concerning mean corpuscular hemoglobin values within control group showed significant decrease were noticed at 5 day, but within treated group, there were no significant differences. mean corpuscular hemoglobin concentration values showed significant decrease ate period 3; 5 and 21 days between control group and treated group respectively. Within control group the significant decrease were noticed obviously at 5 days while within treated group the significant decrease were noticed at 35 days. mean corpuscular volume values showed significant increase at 3 and 5 days period between control and treated groups. Platelets counts within control group showed significant increase at period of 14 days, within treated group the significant increase were recorded at 42 days. Red blood cells showed significant decrease between control and treated groups, while within control group, the significant decrease were at 7 days. White blood cells count within treated group showed significant increase recorded at 21 days. Differential White blood cells count concerning Lymphocytes cells within control group the significant increase were recorded at 42 days  while the significant decrease were noticed at 21 days, but within treated group the significant increase were recorded at 35 days  and significant decrease were noticed at 14 days. Neutrophils counts showed significant decrease at periods 3, 14, 28 and 42 days between control and treated groups respectively; while within control group there were significant decrease at 3 days, but within treated group the significant decrease were noticed at 42 days. Monocytes cells showed significant increase at 35 days between control and treated groups, within control group there were significant increase at 35 days, while within treated group the significant increase recorded at 7 days.

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