scholarly journals The Potential of Iodine Lactoglycerol as an Alternative Fungal Stain in the Laboratory

Biomedika ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 74-82
Author(s):  
Lilis Puspa Friliansari ◽  
Erick Khristian ◽  
Sofie Agustina Hermawanti

Fungi are eukaryotic and opportunistic organisms that can cause damage and disease. Fungi can be identified by direct staining. The composition of the dyes used in fungi staining are toxic, mutagenic and harmful to laboratory personnel and the environment. Consequently, it is necessary to consider use fungi dye continuously, so its need alternative safety dyes to identify fungi in the laboratory. This research aims to determine the potential of iodine lactoglycerol with different concentrations (1.25%, 2.5%, and 5%) as an alternative dye for fungi in the laboratory. The research method used experimental design. The samples originate from moldy bread and made 27 of microscopic slides. The microscopic slides were observed with objective 40x magnification. The parameters assessed in this study were the structure and morphology of fungi including hyphae, conidiophores, vesicles, sterigmata and conidiospores and color intensity using the image analysis of ImageJ. The results showed that various concentrations of iodine lactoglycerol can staining the structure and morphology of the fungi properly and clearly. The value of color intensity of each concentration measured has a different average. The highest average value of color intensity concentration was 5% and the lowest was 1.25%. However, the result of ANOVA test showed a significance of 0.380 (P> 0.05), which means that is no difference significantly from three of various concentrations. The result showed that the difference concentration of iodine lactoglycerol does not affect the color intensity. The conclusion of this study was iodine lactoglycerol solution can be used as an alternative dye or Lactophenol-cotton Blue (LPCB) substitute for fungi identification. It is recommendation for further research be carried out regarding the decrease in the concentration of alternative color solutions below 1.25%, the incubation time for the preparation of the preparations, and the color stability of the prepared mushroom preparations.

Author(s):  
Nurhafizah Nurhafizah ◽  
Rahma Defy Chaniago

This article is written based on the results of research conducted based on the facts that occur in kindergarten Jabal Rahmah Padang, that the cooperative behavior of children has not yet developed due to lact of activities to train cooperative behavior in children. Learning activities in schools are carried out individually by the child, so that it affects the cooperative behavior of children. This study aims to determine the effect of cheerful ball play on the cooperative behavior of children. This research method uses a quantitative approach with a quasi-experimental design. Data collection techniques used are tests, and processed by the difference test (t-test). After the data is processed, it is seen that the children in the experimental class with a cheerful ball game have a higher average value than the control class with a block game. So it can be concluded that the cheerful ball game affects the cooperative behavior of children in kindergarten Jabal Rahmah Padang.


2018 ◽  
Vol 9 (2) ◽  
pp. 385-395
Author(s):  
Krisna Anggraeni ◽  
Devi Afriyuni Yonanda

The purpose of this study was to describe the effectiveness of a teaching materials based on local wisdom in jigsaw learning model to skill of writing description. The method used in this study is a quasi experimental design with two groups: the experimental class and control class. The results showed differences in the skills of writing description between the two classes. In the control class tend to not experience a significant increase, while the experimental class increased. The average value of the current skill of writing description in control class pretest to post-test by 61.25 to 71.40, increased 15.57% with average value of n-gain 0.274 in low category. Meanwhile, the average value in experimental class pretest to post-test by 61.22 to 83.31, increased 36.08% with average value of n-gain 0.570 in medium category. The difference of gain indicates that a teaching materials based on local wisdom in jigsaw learning model effectively used to improve the skills of writing description.   Abstrak Tujuan penelitian adalah untuk mendeskripsikan keefektifan bahan ajar berbasis kearifan lokal dalam model pembelajaran teknik jigsaw terhadap keterampilan menulis deskripsi. Metode penelitian menggunakan eksperimen kuasi dengan desain dua kelompok yaitu kelas eksperimen dan kelas kontrol. Hasil penelitian menunjukkan pada kelas kontrol cenderung tidak mengalami peningkatan yang signifikan, sedangkan pada kelas eksperimen terjadi peningkatan. Nilai rata-rata keterampilan menulis deskripsi kelas kontrol pada prates 61.25 pascates 71.40 peningkatan sebesar 16.57% dengan rata-rata n-gain 0.274 termasuk kategori rendah. Sementara itu, nilai rata-rata keterampilan menulis deskripsi kelas eksperimen saat prates 61,22 pada pascates menjadi 83,31 peningkatan sebesar 36.08% dengan rata-rata n-gain 0.570 termasuk kategori sedang. Perbedaan peningkatan tersebut mengindikasikan bahwa bahan ajar berbasis kearifan lokal dalam model pembelajaran teknik jigsaw efektif digunakan untuk meningkatkan keterampilan menulis deskripsi. Kata Kunci:  Bahan Ajar Berbasis Kearifan Lokal, Model Pembelajaran Teknik Jigsaw, Keterampilan Menulis Deskrispi


Author(s):  
Astri Anisa ◽  
Lina Marlina ◽  
Dedih Wahyudin

The purpose of this research is to study students' abilities in hiwar before and after using Pop Up Card visual media and understand the relationship between the two. This research method is an Experiment with Quasi-Experimental Design. The results of this study are about the ability of students before using visual media Pop Up Cards to achieve sufficient ability with an average value of 63.82. And finally it can reach 70, 00. And the importance of students' ability towards hiwar where the calculated T value is greater than the T table value which is 7.55> 2.00.  الأغراض من هذا البحث هي معرفة قدرة التلاميذ على الحوار   قبل و بعد استخدام بطاقة الوسائل البصرية “Pop Up” ومعرفة الأثر بينهما. والمنهج في هذا البحث هي المنهج التجريبية بتصميم التجريبية القبلية. ومن النتائج المحصولة من هذا البحث هي أن قدرة التلاميذ قبل استخدام بطاقة الوسائل البصرية “Pop Up” تدل على درجة كافية بقيمة المتوسط 63.82. و بعد استخدامها تدل على درجة جيدة بقيمة المتوسط 70.00. و يؤثر في قدرة التلاميذ على الحوار حيث إن قيمة "ت" الحسابية أكبر من قيمة "ت" الجدولية يعني 7.55 > 2.00 .


2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Hanifah Rahmadani ◽  
Arrofa Acesta

Abstract: This study aims to determine the effect of application of the model Problem Based Learning on the understanding of the concept of V grade students of SD Negeri Unggulan Kuningan on the subjects of Social Studies IPS concept of Nature and Artificial in Indonesia. This research is a type of Quasi Experimental Design, while the research design using Nonequivalent Control Group Design. The subjects of this study are all students of VA class which amounted to 25 students and VB which amounted to 27 students in Kuningan Elementary State Elementary School. The results showed that the model of Problem Based Learning influenced the students' concept of understanding. This can be seen from the significant differences between the two sample groups, the difference is the average value of pretest = 53.11 to 79.85 in the posttest of the experimental students' learning result. While the students' learning outcomes control group pretest average value = 49.6 to 59.4 on posttest. And based on t test results, showed that the value of t-count = 6.50 with 95% significance obtained t-tabel = 2.96. Then thitung (6.50) > t-table (2.96), meaning that the model of Problem Based Learning has a significant effect on understanding the concept of students Keywords: Problem Based Learning Model, Understanding Student Concept Abstrak: Penelitian ini bertujuan untuk mengetahui pengaruh penerapan model Problem Based Learning terhadap pemahaman konsep siswa kelas V SD Negeri Unggulan Kuningan pada mata pelajaran IPS konsep Kenampakan Alam dan Buatan di Indonesia. Penelitian ini merupakan jenis Quasi Experimental Design, sedangkan desain penelitiannya menggunakan Nonequivalent Control Group Design. Subjek penelitian ini adalah seluruh siswa kelas VA yang berjumlah 25 siswa dan VB yang berjumlah 27 siswa di SD Negeri Unggulan Kuningan. Hasil penelitian menunjukkan bahwa model Problem Based Learning berpengaruh terhadap pemahaman konsep siswa. Hal tersebut dapat dilihat dari adanya perbedaan yang signifikan antara kedua kelompok sampel, perbedaannya yaitu nilai rata-rata pretest = 53,11 menjadi 79,85 pada posttest hasil belajar siswa kelas eksperimen. Sedangkan hasil belajar siswa kelompok kontrol nilai rata-rata pretest = 49,6 menjadi 59,4 pada posttest. Dan berdasarkan hasil uji t, menunjukan bahwa nilai t-hitung = 6,50 dengan signifikansi 95% diperoleh t-tabel = 2,96. Maka t-hitung (6,50) > t-tabel (2,96), artinya bahwa model Problem Based Learning berpengaruh signifikan terhadap pemahaman konsep siswa. Kata kunci: Model Problem Based Learning, Pemahaman Konsep Siswa


2021 ◽  
Vol 8 (2) ◽  
pp. 242
Author(s):  
Siska Siska ◽  
Safei Safei ◽  
Sri Sulasteri

AbstrakTujuan penelitian ini untuk mengetahui: (1) kemampuan operasi hitung bilangan bulat peserta didik kelas VI sebelum menggunakan media pembelajaran manik-manik warna, (2) kemampuan operasi hitung bilangan bulat peserta didik kelas VI setelah menggunakan media pembelajaran manik-manik warna, dan (3) perbedaan kemampuan operasi hitung bilangan bulat pada peserta didik kelas VI SD Negeri 151 Timbula Kec. Bontotiro Kab. Bulukumba sebelum dan setelah menggunakan media pembelajaran manik-manik warna. Penelitian ini merupakan jenis penelitian experimen dengan pre-experimental design. Teknik pengambilan sampel menggunakan sampel jenuh. Sampel dalam penelitian ini yaitu seluruh peserta didik kelas VI SD Negeri 151 Timbula Kec. Bonto Tiro Kab. Bulukumba yang berjumlah 23 orang. Instrumen yang digunakan adalah tes dan dokumentasi. Data yang terkumpul diolah dengan menggunakan analisis statistik deskriptif. Hasil penelitian menunjukkan bahwa: (1) rata-rata nilai kemampuan operasi hitung bilangan bulat peserta didik sebelum diajar menggunakan media pembelajaran manik- manik warna sebesar 29.57, (2) nilai rata-rata setelah diajar menggunakan media manik-manik warna sebesar 77,83, dan (3) terdapat perbedaan kemampuan operasi hitung bilangan bulat pada peserta didik sebelum dan setelah diajar menggunakan media pembelajaran manik-manik warna. Berdasarkan penelitian ini, dapat disimpulkan bahwa penggunaan media pembelajaran manik-manik warna dapat meningkatkan kemampuan operasi hitung bilangan bulat.AbstractThe purpose of this study was to describe: (1) the ability to operate integer counting of class VI students before using color beads learning media, (2) the ability to operate integer counting of class VI students after using the color beads learning media, and (3) the difference in the operating ability of integer counting in class VI of SD 151 Timbula Kec. Bontotiro Kab. Bulukumba before and after using color beads media. The method used was experimental research with a pre-experimental design. The sample was selected using the saturated technique. The sample of this study was all students of class VI of SD 151Timbula Kec. Bonto Tiro Kab. Bulukumba with a total number of 23 students. The instruments used were tests and documentation. The data analysis technique used was descriptive statistics. The results showed that: (1) the average value of the integer count operation ability of students before being taught using the color beads learning media was 29.57, (2) the average value after being taught using color beads media was 77.83, and (3) there were differences in the ability to operate integer count operations of students before and after being taught using color. From this study, it could be concluded that the use of color beads learning media could improve the ability of integer arithmetic operations.


2016 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Anne Anita Permatasari

This research aimed to improve the students motivation and ability learning outcomes using media that is PowerPoint multimedia.The research method used was Quasi Experimental Research One Group Pretest Posttest Design model with a sample of students of class VII SMP I Tarogong Kaler Garut. The research concludes that there is a significant effect on the use of multimedia PowerPoint to upgrade students learning outcomes. It improves that the average increase understanding of writing short stories after being given a multimedia learning using PowerPoint. The average value of the pretest is 63.19 increased to an average value of 77.14 at posttest measurements. The average gain in the interval of 0.30 s / d 0.70 , which means an increase in to learning ability after using PowerPoint multimedia fall into the middle category. Results found the difference in value of the gain calculation pretest and postest value is 20. The lowest score on the pretest to posttest is increased from the value of 53 to 58. The average value of the value increased from 63 to 76. The increase in the highest value reached 78 %, the lowest increase in five %, an average increase to 35%. Classification increase in the high category of 8.4 %, 55.5 % middle category, and a lower category by 36.1 %.


2020 ◽  
Vol 11 (2) ◽  
pp. 70
Author(s):  
Aida Nursanti

This research was motivated by the low ability of students to read at the beginning, so the researchers applied the SAS method in order to help students who still had difficulty reading. This study aims to determine the difference between the pretest and posttest by applying the synthetic analytical structural method (SAS) in reading the beginning of grade II elementary school students and to determine the increase in the pretest and posttest in reading the beginning of students. This type of research is a type of pretest-posttest one group pre-experimental design, which is an experiment that is only carried out in one group, without comparison. This research was carried out in class II SDN 004 Baturijal Hulu, Peranap sub-district with a total of 29 students. Based on the research results, it was found that the average value of reading ability before being treated (pretest) was 51.68 while the average value of reading ability after treatment (posttest) was 88.65 and the average value of normality gain was 0.76 which including the high category and the results of hypothesis testing using t-test with t = 47.191 and t table = 2.045, it is concluded that t count> t table, then reject H0 means significant. This means that there is a significant increase in pre-test reading in grade II students between before (pretest) and after (posttest) applying the Synthetic Structural Analytical (SAS) method.


Author(s):  
Yunita Yunita ◽  
Hidayat Hidayat ◽  
Harun Sitompul

This study aims to: (1) investigate the effect of Jigsaw cooperative learning on students learning outcomes; (2) find the difference in learning outcomes between high and low learning motivation and (3) find the interaction between learning approaches and learning motivation towards learning outcomes. The population of the study is students of grade IVa, IVb, IVc at SD Kasih Ibu Patumbak and the sample in this study is grade IVa with 35 students and grade IVb with 35 students. The results show that: (1) the average student learning outcomes of jigsaw cooperative learning is 28.40 while conventional is 24.14. Thus, students learning outcomes that get cooperative learning of jigsaw type are higher than conventional learning, (2) Students who have high motivation get an average value = 30.74, while low motivation is 22.72. Thus, it can be concluded that there are differences in student learning outcomes having high learning motivation and low learning motivation, and (3) students learning outcomes  taught by jigsaw cooperative learning are high learning motivation groups (32.94), and low learning motivation groups (24.58), while students taught with conventional learning are high learning motivation groups (28.40 ), and low motivation groups (20,95). Thus, there is no interaction between learning approaches and learning motivation towards learning outcomes.


Manuskripta ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 67
Author(s):  
Novarina Novarina

Abstract: This research is a comparative literary study that uses Malay and Javanese versions of Mahabarata text sources. The research objects used were the text edition of Pandhawa Gubah (PG) by Sudibjo Z. Hadisutjipto and the text of Cheritera Pandawa Lima (CPL) by Khalid Hussain. The research method used is descriptive-analysis method. In the comparative study used a comparative literary theory proposed by Endraswara (2011). The results of the text comparison reveal the similarities and differences in the image of Bima figures in the Javanese and Malay versions. The equation as a whole is that both texts contain the same heroic storyline and heroic character, Bima. In addition, Indian influence is still evident in the two texts seen from the nuances of Hinduism that exist in both texts. While the difference is seen in the events that accompany Bima's struggle in achieving his victory. Based on these similarities and differences, it can be seen that the authors attempt to represent the concept of metaphysical interactions vertically and horizontally expressed through PG text. --- Abstrak: Penelitian ini adalah satu kajian sastra bandingan yang menggunakan sumber teks Mahabarata versi Melayu dan Jawa. Objek penelitian yang digunakan adalah edisi teks Pandhawa Gubah (PG) karya Sudibjo Z. Hadisutjipto dan teks Cheritera Pandawa Lima (CPL) karya Khalid Hussain. Metode penelitian yang digunakan adalah metode deskriptif-analisis. Dalam telaah perbandingan digunakan teori sastra bandingan yang dikemukakan Endraswara (2011). Hasil perbandingan teks mengungkapkan adanya persamaan dan perbedaan citra tokoh Bima dalam versi Jawa maupun versi Melayu. Persamaan secara keseluruhan adalah kedua teks tersebut mengandung alur cerita kepahlawanan dan tokoh pahlawan yang sama yaitu Bima. Selain itu, pengaruh India masih tampak dalam kedua teks tersebut dilihat dari nuansa Hinduisme yang ada dalam kedua teks. Sementara perbedaannya tampak pada peristiwa-peristiwa yang menyertai perjuangan Bima dalam mencapai kemenangannya. Berdasarkan persamaan dan perbedaan tersebut tampak adanya upaya penulis untuk merepresentasikan konsep interaksi metafisik secara vertikal dan horizontal yang diungkapkan melalui teks PG.


2017 ◽  
Vol 1 (1) ◽  
pp. 89
Author(s):  
Indah Purwaningsih ◽  
Supriyanto Supriyanto

Abstract: Chlorine is a green halogen-shaped halogen gas at normal temperature and serves as bleach, stain remover and disinfectant. Chlorine is now widely used for bleaching rice so that less quality rice looks like quality rice. Chlorine is very toxic and causes mucous membrane irritation, highly reactive and very powerful oxidizer. The purpose of this research was to determine the difference of chlorine level in chlorinated rice washed once, twice and 3 times. The sample in this study amounted to 11 samples calculated by replication formula. Each sample was treated 3 times, ie 1 washed once, 2 washed twice and washed 3 times. The samples then examined by iodometric titration method. Based on the results of the study using ANOVA test, 11 samples obtained the average value of chlorine after washed once amount of 0.0176%, after washed twice amount of 0.0111%, and after washed 3 times amount of 0.0052% with the value significance p = 0.03 (p <0.05) at 95% confidence level which means there was a significant difference between chlorine levels in chlorinated rice washed once, twice and 3 times.Abstrak: Klorin merupakan unsur halogen berbentuk gas berwarna kuning kehijauan pada suhu normal danberfungsi sebagai pemutih, penghilang noda maupun desinfektan. Klorin sekarang banyak digunakan untuk bahan pemutih beras agar beras yang kurang berkualitas tampak seperti beras berkualitas. Klorin sangat toksik dan menyebabkan iritasi membran mukosa, sangat reaktif dan merupakan oksidator yang sangat kuat. Tujuan dari penelitian ini ialah untuk mengetahui perbedaan kadar klorin pada beras berklorin yang dicuci sebanyak 1 kali, 2 kali dan 3 kali. Sampel dalam penelitian ini berjumlah 11 sampel yang dihitung dengan rumus replikasi. Setiap sampel diberi perlakuan sebanyak 3 kali, yaitu 1 kali pencucian, 2 kali pencucian dan 3 kali pencucian. Sampel penelitian kemudian diperiksa dengan metode titrasi iodometri. Berdasarkan hasil penelitian menggunakan uji Anova secara komputerisasi terhadap 11 sampel diperoleh nilai rata-rata kadar klorin setelah 1 kali pencucian sebesar 0,0176 %, setelah 2 kali pencucian sebesar 0,0111 %, dan setelah 3 kali pencucian sebesar 0,0052 % dengan nilai signifkansi p = 0,03 (p<0,05) pada tingkat kepercayaan 95% yang artinya ada perbedaan yang bermakna antara kadar klorin pada beras berklorin yang dicuci sebanyak 1 kali, 2 kali dan 3 kali.


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