scholarly journals FORMULASI EKSTRAK KLOROFORM DAUN BELIMBING WULUH DALAM BENTUK SEDIAAN GEL dan UJI AKTIVITAS ANTIBAKTERI TERHADAP Staphylococcus epidermidis

2019 ◽  
Vol 1 (3) ◽  
pp. 203-209
Author(s):  
Verawaty Tan ◽  
Nova Tri Rahmadhani ◽  
Irene Puspa Dewi
Keyword(s):  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Chloroform Extract ◽  
Significant Difference ◽  
Agar Media ◽  
The One ◽  
Dark Green ◽  
Homogeneous Good

Herbal treatments are needed to treat various diseases, one of which is the leaves of Belimbing Wuluh (Averrhoa bilimbi L.). The aim of this study was to formulate chloroform extract of Belimbing Wuluh’s leaf (Averrhoa bilimbi L.) into gel preparations that were good, effective, and safe to use and to determine the antibacterial activity of chloroform extract of Belimbing Wuluh (Averrhoa bilimbi L.) leaf extract to Staphylococcus epidermidis by well method. The results showed that form of gel is a thick, dark green, the distinctive smell of Wuluh starfruit leaf extract, homogeneous, good skin pH, good washing and does not irritate the skin. This study uses Nutrient Agar media as a culture medium for Staphylococcus epidermidis bacteria. The results indicate that the chloroform extract gel leaves of belimbing wuluh leaves in formula 1 (10%), formula 2 (20%), and formula 3 (30%) were able to inhibit the growth of Staphylococcus epidermidis bacteria. In formula 3 (30%) gel extract of belimbing wuluh leaves has the widest zone of inhibition compared to other dosage formulas. The statistical test using the One Way ANOVA test showed a significant difference from the average diameter of the inhibition zone between all concentrations of chloroform extract gel leaves of belimbing wuluh leaves that were significant with (α ˂ 0.05).

scholarly journals EFEKTIVITAS GEL EKSTRAK ETANOL BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Staphylococcus aureus

2020 ◽  
Vol 2 (2) ◽  
pp. 105-112
Author(s):  
Irene Puspa Dewi ◽  
Ilham Maslan Orde ◽  
Verawaty Verawaty
Keyword(s):  
Staphylococcus Aureus ◽  
Allium Sativum ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Traditional Medicines ◽  
Significant Difference ◽  
Irritation Test ◽  
Gel Preparation ◽  
The One

Garlic (Allium sativum L.) is one of the traditional medicines which has an antibacterial efficacy compound namely Allisin which is able to inhibit the growth of Staphylococcus aureus bacteria. This study aims to formulate the garlic bulb extract into a good gel preparation for use as an acne drug and to determine the antibacterial effectiveness of the ethanol extract of garlic gel against the bacterium Staphylococcus aureus using the wells method. The gel evaluation was carried out to meet the requirements with organoleptic test parameters, homogeneity, pH, spreadability, adhesion and irritation test. This study uses extracts of 10% and 20% concentrations, at each concentration being able to inhibit the growth of Staphylococcus aureus bacteria. In the formula, the concentration of 20% has a inhibition zone diameter of 1.59 cm and a concentration of 10% has a inhibition of 1.50 cm. Statistical test results using the One Way ANOVA test found that there was no significant difference between the average diameter of inhibition of garlic extract gel concentration of 10% with a concentration of 20%.

scholarly journals The Effectiveness Of The Papaya Leaf Extract (Carica Papaya L) On The Growth Of Epidermidic Staphylococcus Bacteria

2020 ◽  
Vol 11 (1) ◽  
pp. 65
Author(s):  
Charles Manurung ◽  
Melysa Loeslim ◽  
Setia Budi Tarigan
Keyword(s):  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Sampling Method ◽  
Inhibition Zone ◽  
Inhibitory Zone ◽  
Positive Bacterium ◽  
Post Test ◽  
The One ◽  
Post Hoc ◽  
One Way Anova

This research was conducted to determine the effectiveness test of papaya leaf extract with a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 90% and 100% against staphylococcus epidermidis bacteria. Staphylococcus epidermidis is a gram-positive bacterium in the form of cocci and is harmful to humans. This research was conducted with an experimental method with a post-test only design and sampling with a purposive sampling method. The investigation of the effectiveness of papaya leaf extract against staphylococcus epidermidis was done by diffusion using paper discs, namely by calculating the diameter of the bacterial inhibition zone of paper discs that have been moistened with papaya leaf extract. The results of this study were analyzed out with the One-Way ANOVA test followed by the Post Hoc Test. The test showed that there were significant differences from each treatment given with a 95% confidence index. The bacteria treated with papaya leaf extract 100% against staphylococcus epidermidis had the highest average inhibitory zone power compared to other concentrations of 14.97 mm. The papaya leaf extract 100% has the best antibacterial effect compared to other concentrations because the best inhibitory zone power is found on those extract concentrations.

scholarly journals GEL ANTI JERAWAT EKSTRAK DAUN BUTA-BUTA (Excoecaria agallocha L.) DAN PENGUJIAN ANTIBAKTERI Staphylococcus epidermidis

2015 ◽  
Vol 1 (2) ◽  
pp. 65-72
Author(s):  
Ika Olivia Borman ◽  
Yusriadi Yusriadi ◽  
Evi Sulastri
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Diffusion Method ◽  
Activity Data ◽  
Excoecaria Agallocha ◽  
Significant Difference ◽  
The Stability

Buta-Buta (Excoecaria agallocha L.) leaf is a type of mangrove plants that has antibacterial activity againtsbacteriacausing acne, Staphylococcus epidermidis,. This study aims to formulate buta-buta leaf extract with the concentration of 12.5 % into anti-acne gel using two gels, namely HPMC 8% (Formula 1) and Carbopol 940 2%(Formula 2). Antibacterial activity test was carried out by agar diffusion method using well. The stability and antibacterial activity data during 28 days were analyzed statistically using unpaired t-student with a degree of confidence of 95% (α = 0.05). The evaluation results of anti-acne gel form of buta-buta leaf extract in Formula 2 showedbetter characteristics than Formula 1, which include organoleptic examination, homogenity, pH and viscosity during 28 days. The antibacterial test showed the diameter of inhibition zone of Formula 2 is 11.03 mm ±0,01. The statistic analysis showed a significant difference between Formula 2 with a positive control (klindamisin gel).

scholarly journals Potensi Daun Trembilungan (Begonia hirtella Link) sebagai Antibakteri dan Antifungi

Biosfera ◽  
2017 ◽  
Vol 33 (3) ◽  
pp. 126
Author(s):  
Febri Nur Ngazizah ◽  
Nuraeni Ekowati ◽  
Aisyah Tri Septiana
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Inhibitory Zone ◽  
Ethyl Palmitate ◽  
E Coli ◽  
Significant Difference

Begonia hirtella leaf are commonly used by people in mountainous areas to treat itchy due to insect bites and skin infection. This study aims to (1) determine proper solvent extract of B. hirtella leaf on antibacterial activity of S. aureus and E. coli and on antifungal activity of C. albicans (2) determine the lowest concentration of leaf extract of B. hirtella that indicate the formation of inhibition zone (3) know the content of bioactive compounds contained in each leaf extract of B. hirtella. The method used in this study is experimental with completely randomized design (CRD). The treatments were type of solvents (n-hexane, ethyl acetate, ethanol and water) and a serie of the ethyl acetate extract concentration of 500 ppm, 450 ppm, 400 ppm, 350 ppm, 300 ppm and 250 ppm against S. aureus, E. coli and C. albicans. Data were analyzed using analysis of variance (Anova) and the significant differences between the treatments were analyzed by Duncan test at 95% confidence level. The result showed that each solvent extracts affect microbes growth with a highly significant difference p <0.05. Solvent extracts that have the best inhibitory zone is ethyl acetate against S. aureus with an average diameter of inhibitory zone 13.75 ± 1.26 mm. Increasing concentrations of ethyl acetate extract of 250 ppm to 500 ppm increase inhibition zone against microbes. The lowest concentration that show inhibition zone was 300 ppm. The formation inhibition of zone on microbes growth happened due to their compounds in the extract. Extract of n-hexane contains stigmasterol, ethyl acetate contains neophytadiene, while the ethanol extract contains ethyl palmitate

scholarly journals Anti Acne Vulgaris Topical Cream From Combination Extract of Gymnanthemum Amygdalinum Del. and Elaeis Guineensis Jacq. Leaves

2020 ◽  
Vol 3 (1) ◽  
pp. 42-50
Author(s):  
Wahyudi ◽  
Ayu Syahfitri ◽  
Syukur Berkat Waruwu ◽  
Dian Ika Perbina
Keyword(s):  
Antibacterial Activity ◽  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Acne Vulgaris ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Propionibacterium Acne ◽  
Self Confidence ◽  
Adolescents And Adults

Acne is one problem in adolescents and adults who can reduce self-confidence. This study aimed to formulate the combination cream of African leaf extract and palm oil leaves to determine the antibacterial activity of African leaf extract against several acne-causing bacteria (Propionibacterium acne and Staphylococcus epidermidis). Test antibacterial activity of extracts and cream with disc diffusion method. The cream is made with concentration of 5, 10 and 20% w/w. The evaluation results of cream with concentration of 5, 10 and 20% were physically stable for 4 weeks of storage at temperature room, homogeneous, pH and it did not cause irritation. Based on the test results, a combination of African leaf extract with concentration of 5% effectively inhibited bacterial growth with an average inhibition zone diameter of 12.06 mm for Propionibacterium acne bacteria and 13.63 mm for Staphylococcus epidermidis bacteria. The result of this study suggest that combination of African leaf extract and palm leaves posses potent anti-bacterial activity against Propionibacterium acne and Staphylococcus epidermidis.

scholarly journals Antibacterial Effect Of Kaempferia Galanga L Extract On Lactobacillus Acidophilus –In Vitro

2017 ◽  
Vol 1 (01) ◽  
pp. 22
Author(s):  
Josi Saraswati ◽  
Annisa Septalita ◽  
Arini Bovita. N
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Acidophilus ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Inhibition Effect ◽  
Kaempferia Galanga ◽  
Significant Difference ◽  
Agar Media ◽  
Negative Controls

Introduction: Lactobacillus acidophilus is one of the bacteria causes dental caries. The previous study has shown that Kaempferia galanga extract has a potential to inhibit the growth of Lactobacillus acidophilus.Objective: To determine the antibacterial effect of Kaempferia galanga extract to Lactobacillus acidophilus.Methods:Kaempferia galanga is extracted in 3 different solvents:dichlormethane, ethanol, and aquades. For each solvent, 0.2 μl Kaempferia galanga extractdroped into 6 mm steril paper dics. 0.1 ml Lactobacillus acidophilus inoculated on MRS agar. Each disc contains extract were impragnated into the agar media, then incubated at 370C for 24 hours, and inhibition zone measured.Results: Mean scores of Kaempferia galanga extract in 3 different solvents are: Kaempferia galanga (dichlormethane) is 1.6400; Kaempferia galanga (ethanol) is 1.7440; Kaempferia galanga extract is 1.6600; boiled Kaempferia galanga is 1.7000. Using Mann-Whitney Test, the results are: negative controls have no inhibition effect on Lactobacillus acidophilus compaired to Kaempferia galanga extract, comparation of those 4 Kaempferia galangal treatments shows no significant difference, those 4 Kaempferia galanga treatments compaired to erythromycin antibacterial effect shows significant difference, otherwise 4 Kaempferia galanga treatments compaired to penicillin shows no significant difference except Kaempferia galanga (ethanol).Conclusions: Kaempferia galanga extract can kill Lactobacillus acidophilus. Inhibition effect of Kaempferia galanga extract has no significant difference to penicillin but lower inhibition effect than erythromycin. The Kaempferia galanga extracts showed better antibacterial activity than penicillin.

scholarly journals Efektivitas campuran ekstrak daun kari (Murraya koeginii) dengan daun sirsak (Annona murricarta linn) sebagai insektisida dalam menghentikan larut rumah (Musca domestica)

2021 ◽  
Vol 2 (2) ◽  
pp. 142
Author(s):  
Syahrizal Syahrizal ◽  
Junaidi Junaidi
Keyword(s):  
Musca Domestica ◽  
Leaf Extract ◽  
Human Life ◽  
P Value ◽  
Annona Muricata ◽  
House Flies ◽  
Curry Leaves ◽  
Significant Difference ◽  
The One ◽  
One Way Anova

Background: Flies (Musca domestica) can act as vectors of typhus, dysentery, and cholera. Various methods have been used to control flies from around human life, either mechanically (light traps), a chemical in the form of toxic baits or insecticides, or with scents that are repellent. A mixture of curry leaf extract with soursop leaves can be used as an insecticide to repel house flies. Because curry leaves and soursop leaves contain compounds, tannins, saponins, and flavonoids that flies do not like.Objective: The purpose of this study was to determine the effectiveness of a mixture of curry leaves with soursop leaves as a natural insecticide in repelling the flies (Musca Domestica).Methods: This research is an experimental design with a mixture of curry leaf extract with soursop leaves on the power of repelling house flies. This research was conducted in 2019 in Darul Kamal District, Aceh Besar. The object of the research was 450 house flies, which consisted of five treatments with three repetitions. Data analysis was carried out with the one-way ANOVA statistical test.Results: The descriptive results showed that at a dose of 10gr/100ml the average flies were able to repel 37 flies, at a dose of 20 gr/100ml were 44, at a dose of 30 gr/100ml were 59 and at a dose of 40 gr/100ml was 70. Of the four treatments, the most effective dose was at a dose of 40gr/100ml, which was 70 (77.7%). Statistically, it showed that there was a significant difference (p < 0.05) between various doses/treatments of mixed extracts of curry leaves (Murraya koeginii) and soursop leaves (Annona muricata Linn) on the repelling power of house flies (Musca domestica), with p-value = 0.000.Conclusion: A mixture of curry leaf extract (Murraya koeginii) with soursop leaf (Annona muricata Linn) is effective as an insecticide in repelling house flies (Musca domestica).

scholarly journals Konsentrasi Hambatan Minimum ekstrak Portulaca grandiflora terhadap penyakit Motile Aeromonad Septicaemia

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Dafid Lauluw ◽  
Reni L. Kreckhoff ◽  
Sammy N.J. Longdong ◽  
Desy M. Mantiri ◽  
Juliaan Ch. Watung ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Agar Medium ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Clear Zone ◽  
Minimum Concentration ◽  
Portulaca Grandiflora ◽  
Standard Classification ◽  
Classification Table ◽  
Paper Disc

The objective of this study was to determine the minimum concentration of extract in inhibiting motile motile aeromonad septicaemia. The material used was the stems and leaves of the plant P. grandiflora as much as 10 g. The crushed simplicia was then macerated with aquades with a ratio of 1:10,  then filtered with filter paper and, put in an Erlenmeyer, and evaporated in an incubator at temperature of 30°C. The sensitivity test was performed by placing a paper disc containing extract on the agar medium that had been scratched with bacterial colonies. Clear zone formed around the paper disc was compared with standard classification table for clarifying the inhibition level.  The results showed that stem and leaf extract had inhibition zone against Aeromonas hydrophila bacteria that caused motile aeromonad septicaemia. The best result was obtain at concentration of extract 3.25 mg / L) with an average diameter of 22.67 mm and for leaf extract had a moderate to strong zone for all treatments. These proved that stem and leaf extract of P. grandiflora plant had antibacterial activity and can be used as control of MAS disease caused by A. hydrophila bacteria. Keywords:  Portulaca grandiflora plant, motile aeromonad septicemia,  minimum       inbibition concentration

scholarly journals ANTIBACTERIAL ACTIVITY OF MIANA LEAF ESTRACT (Coleus atropurpereus) ON Steptococcus aureus

2020 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Anita Anita ◽  
Mujahidah Basarang ◽  
Rahmawati Rahmawati
Keyword(s):  
Antibacterial Activity ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Laboratory Observation ◽  
Extract Concentration ◽  
Observation Method ◽  
The One ◽  
Growth Of Bacteria

                                               ABSTRACTMiana leaf(Coleus atrropurpureus) is the one of biofarmacology plant which using for antibacterial activity.Miana leaf contains flavonoid, tanin, saponin  which can inhibit growth of bacteria. The aims this research were study antibacterial activity of miana leaf extract on Streptococcus aureus.This research used laboratory observation method which used miana leaf extract concentration 250 mg/ml, 125 mg/ml,  62.5 mg/ml, 31,25 mg/ml, and 15,625 mg/ml. This research used tetracylin for positive control and aquadest for negative control which growth 0n 37o C  used difusion agar method. The results showed antibactrila activity  miana leaf  extract with a concentration 250 mg/ml with inhibition zone 0.00 mm, 125 mg/ml with inhibition zone 0,00 mm, 62,5 mg/ml with inhibition zone 0,00 mm, 31,25 mg/ml with inhibition zone 0,00 mm, and 15,62 mg/ml with inhibition zone 0,00 mm. For positive control showed inhibition zone 27,6 mm and negative control with inhibition zone 0,00 mm. Miana leaf extract concentration 250 mg/ml, 125 mg/ml, 62,5 mg/ml, 31,25 mg/ml and 15,62 mg/ml were not effectively inhibit Streptococcus sp.

scholarly journals Efektivitas Antifungi Ekstrak Daun Patikan Kebo (Euphorbia hirta) Terhadap Jamur Penyebab Kandidiasis (Candida albicans)

2019 ◽  
Vol 2 (1) ◽  
pp. 22
Author(s):  
Zulkarnain Zulkarnain ◽  
Cut Muthiadin ◽  
Fatmawati Nur ◽  
Rusmadi Rukmana
Keyword(s):  
Infectious Disease ◽  
Candida Albicans ◽  
Leaf Extract ◽  
Microbial Growth ◽  
Inhibition Zone ◽  
Euphorbia Hirta ◽  
Randomized Design ◽  
Paper Disk ◽  
Significant Difference ◽  
Completely Randomized Design

<em>Candidiasis is an infectious disease caused by the fungus Candida albicans. This  study aims to determine the effect of patikan kebo (Euphorbia hirta) leaf extract in inhibiting the growth of C. albicans. This research is an experimental study which was compiled using a Completely Randomized Design (CRD) by giving Patikan Kebo (E. hirta) leaf extract  in several concentrations, the concentrations of 40%, 45%, 50%, 55%, and 60% and controls (aquadest) and C. albicans mushroom with three repetitions. The parameters measured are the amount of inhibitory diameter formed around the paper discs in the 24 hour and 48 hour period. D ata obtained with three repetitions and observations of 24 hours and 48 hours showed that giving patikan kebo leaf extract in several types of concentration had an effect on inhibiting the growth and fungi of C . albicans. Furthermore, the data obtained were analyzed using analysis of variance (F-test) at the level of α 0.05 and continued using the Smallest Significant Difference (LSD) α 0.05 where the results showed that the giving of patikan kebo extract significantly affected the microbial growth test (C. albicans), where the higher the concentration of patikan kebo leaf extract, the greater the inhibition zone formed around the paper disk. </em>

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