Antioxidant, Antibacterial and Dyeing Potential of Crude Pigment Extract of Gonatophragmium triuniae and Its Chemical Characterization

Filamentous fungi synthesize natural products as an ecological function. In this study, an interesting indigenous fungus producing orange pigment exogenously was investigated in detail as it possesses additional attributes along with colouring properties. An interesting fungus was isolated from a dicot plant, Maytenus rothiana. After a detailed study, the fungal isolate turned out to be a species of Gonatophragmium belonging to the family Acrospermaceae. Based on the morphological, cultural, and sequence-based phylogenetic analysis, the identity of this fungus was confirmed as Gonatophragmium triuniae. Although this fungus grows moderately, it produces good amounts of pigment on an agar medium. The fermented crude extract isolated from G. triuniae has shown antioxidant activity with an IC50 value of 0.99 mg/mL and antibacterial activity against Gram-positive bacteria (with MIC of 3.91 μg/mL against Bacillus subtilis, and 15.6 μg/mL and 31.25 μg/mL for Staphylococcus aureus and Micrococcus luteus, respectively). Dyeing of cotton fabric mordanted with FeSO4 using crude pigment was found to be satisfactory based on visual observation, suggesting its possible use in the textile industry. The orange pigment was purified from the crude extract by preparative HP-TLC. In addition, UV-Vis, FTIR, HRMS and NMR (1H NMR, 13C NMR), COSY, and DEPT analyses revealed the orange pigment to be “1,2-dimethoxy-3H-phenoxazin-3-one” (C14H11NO4, m/z 257). To our understanding, the present study is the first comprehensive report on Gonatophragmium triuniae as a potential pigment producer, reporting “1,2-dimethoxy-3H-phenoxazin-3-one” as the main pigment from the crude hexane extract. Moreover, this is the first study reporting antioxidant, antibacterial, and dyeing potential of crude extract of G. triuniae, suggesting possible potential applications of pigments and other bioactive secondary metabolites of the G. triuniae in textile and pharmaceutical industry.

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Plant antimicrobial peptides: structures, functions, and applications

Botanical Studies ◽

10.1186/s40529-021-00312-x ◽

2021 ◽

Vol 62 (1) ◽

Author(s):

Junpeng Li ◽

Shuping Hu ◽

Wei Jian ◽

Chengjian Xie ◽

Xingyong Yang

Keyword(s):

Antimicrobial Activity ◽

Antimicrobial Peptides ◽

Agricultural Production ◽

Food Additives ◽

Antimicrobial Activities ◽

Gram Positive Bacteria ◽

Potential Applications ◽

Potential Applicability ◽

Bacteria And Fungi ◽

Positively Charged

AbstractAntimicrobial peptides (AMPs) are a class of short, usually positively charged polypeptides that exist in humans, animals, and plants. Considering the increasing number of drug-resistant pathogens, the antimicrobial activity of AMPs has attracted much attention. AMPs with broad-spectrum antimicrobial activity against many gram-positive bacteria, gram-negative bacteria, and fungi are an important defensive barrier against pathogens for many organisms. With continuing research, many other physiological functions of plant AMPs have been found in addition to their antimicrobial roles, such as regulating plant growth and development and treating many diseases with high efficacy. The potential applicability of plant AMPs in agricultural production, as food additives and disease treatments, has garnered much interest. This review focuses on the types of plant AMPs, their mechanisms of action, the parameters affecting the antimicrobial activities of AMPs, and their potential applications in agricultural production, the food industry, breeding industry, and medical field.

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Graphene nanocomposites: A review on processes, properties, and applications

Journal of Industrial Textiles ◽

10.1177/15280837211024252 ◽

2021 ◽

pp. 152808372110242

Author(s):

Kadir Bilisik ◽

Mahmuda Akter

Keyword(s):

Textile Industry ◽

In Situ Polymerization ◽

Resin Transfer Molding ◽

Single Layer ◽

Chemical Vapor ◽

Synthesis Process ◽

Graphene Nanocomposites ◽

Transfer Molding ◽

Potential Applications ◽

Nano Graphene

In this paper, graphene, graphene/matrix, and graphene/fiber nanocomposites, including their synthesis process, fabrication, properties, and potential applications, were reviewed. It was found that several synthesis techniques for nanographene were developed, such as liquid-phase exfoliation and chemical vapor deposition. In addition, some fabrication processes of graphene/matrix and graphene/fiber-based nanocomposites were made, including in-situ polymerization, nanostitching in that single layer nano graphene plate could be interconnected by means of carbon nanotube stitching, resin transfer molding, and vacuum-assisted resin transfer molding. Several properties, including mechanical, thermal, and electrical, on the graphene nanoplatelets materials were summarized in this review paper. It was realized that graphene, graphene/matrix, and graphene/fiber nanocomposites have extraordinary mechanical, thermal, and electrical properties used in advanced engineering applications, including soft robotics, microelectronics, energy storage, biomedical and biosensors as well as textile industry.

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Influence of Nano Silica Coating on the Functional Properties of Cotton Fabrics

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.67.149 ◽

2009 ◽

Vol 67 ◽

pp. 149-154

Author(s):

K. Sasipriya ◽

N. Gobi ◽

R. Palanivelu ◽

T.V. Ramachandran ◽

V. Rajendran

Keyword(s):

Textile Industry ◽

Silica Coating ◽

Cotton Fabrics ◽

Air Permeability ◽

Wall Material ◽

Nano Silica ◽

Chemical Route ◽

Wrinkle Resistance ◽

Potential Applications ◽

Nanosilica Particles

Coating of nanoparticles on fabrics provides huge potential applications in textile industry. The microencapsulation process is used to encapsulate the nanosilica particles which is used to coat on the surface of fabrics and to observe the special properties such as anti-bacterial, wrinkle resistance, etc. The amorphous nano silica particles were prepared from the natural resources through chemical route. The encapsulated nano silica was prepared using sodium alginate as a wall material by the coacervation method. The prepared sample was coated on the surface of the fabrics by pad-dry-cure method. The anti-bacterial studies were carried out for the nano silica coated and uncoated fabrics and the results would demonstrate the antibacterial effectiveness of treated cotton fabrics. The basic properties like tensile strength, tear strength, air permeability, crease recovery and whiteness index have been analysed for the coated and uncoated fabrics.

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Novel Biobased Textile Fiber from Colombian Agro-Industrial Waste Fiber

Molecules ◽

10.3390/molecules23102640 ◽

2018 ◽

Vol 23 (10) ◽

pp. 2640 ◽

Cited By ~ 1

Author(s):

Maria Amaya Vergara ◽

Melissa Cortés Gómez ◽

Maria Restrepo Restrepo ◽

Jorge Manrique Henao ◽

Miguel Pereira Soto ◽

...

Keyword(s):

Textile Industry ◽

Industrial Waste ◽

Degree Of Polymerization ◽

Cellulose Content ◽

Short Fibers ◽

Cellulose Pulp ◽

Potential Applications ◽

Fique Fibers ◽

Regenerated Fibers ◽

Long Fibers

Fique fibers, native to Colombia, are traditionally used for ropes and bags. In the extraction of long fibers for these purposes, the same amount of short fibers is generated; the short fibers are then discarded in the soil or in landfills. This agro-industrial waste is cellulose-rich and can be potentially developed into new biobased products. As an alternative use for these fibers, viscose regenerated fibers with potential applications in the textile industry were developed. Fique waste fibers were pulped (to produce fique cellulose pulp, FCP) using a 33 design of experiment (DOE) to adjust the variables of the whitening treatment, and DOE analysis showed that time and hydrogen peroxide concentration do not have a significant effect on non-cellulosic remotion, unlike temperature. The behavior of this pulp in the production of viscose was compared against that of commercially available wood cellulose pulp (WCP). FCP showed a suitable cellulose content with a high degree of polymerization, which makes it a viable pulp for producing discontinuous viscose rayon filaments. Both pulps showed the same performance in the production of the viscose dope and the same chemical, thermal, and mechanical behavior after being regenerated.

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Wastewater effluent characteristics from Moroccan textile industry

Water Science & Technology ◽

10.2166/wst.2013.205 ◽

2013 ◽

Vol 67 (12) ◽

pp. 2791-2799 ◽

Cited By ~ 7

Author(s):

Y. Mountassir ◽

A. Benyaich ◽

M. Rezrazi ◽

P. Berçot ◽

L. Gebrati

Keyword(s):

Textile Industry ◽

Chemical Characterization ◽

Textile Wastewater ◽

Complete Characterization ◽

Wastewater Effluent ◽

The European Union ◽

Physico Chemical ◽

Standard Limit ◽

Effluent Characteristics

The objectives of this work were to carry out a complete characterization of textile wastewater, resulting from a textile unit located in the Marrakesh region. A physico-chemical characterization has been performed, focused on organic and toxicological aspects. The cladoceran Daphnia magna was used as the sensor organism and lethal concentration as a criterion to measure the toxicity of textile wastewater. The physico-chemical and toxicological status of a local textile effluent showed considerable values limitation, when compared to the European Union standard limit and Moroccan guide level and other studies. In view of those characteristics, the wastewater effluent from the textile industry should be considered to be treated before discharge to the environment.

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Total phenolic and flavonoid compounds, antioxidant and toxicity profile of extract and fractions of paku atai tuber (Angiopteris ferox Copel)

Food Research ◽

10.26656/fr.2017.3(6).135 ◽

2019 ◽

pp. 734-740

Author(s):

Nur S. ◽

F. Mubarak ◽

C. Jannah ◽

D.A. Winarni ◽

D.A. Rahman ◽

...

Keyword(s):

Antioxidant Activity ◽

Crude Extract ◽

Iron Reduction ◽

Colorimetric Method ◽

Total Phenolic ◽

Toxicity Profile ◽

Flavonoid Content ◽

Ic50 Value ◽

Phenolic And Flavonoid ◽

Phenolic And Flavonoid Compounds

Paku atai plant (Angiopteris ferox Copel), specifically the tuber has been used empirically as an anticancer and antidote materials by Dayaknese in West Kutai, with limited scientific study. Thus, this research had been conducted to determine and evaluate the total phenolic, flavonoid content of this plant along with the antioxidant and toxicity profile. The research used several extracts’ solvents including extraction with ethanol (crude extract, CE) and subsequently partitioned with n-hexane (HF); ethyl acetate (EF) and ethanol aqueous (EAF). The resulted crude extract and fractions were then analyzed through colorimetric method to determine the phenolic and flavonoid total; with DPPH and FRAP to observe the antioxidant activity; and using BSLT method to evaluate the toxicity activity. The results showed that the EF fraction provided the strongest antioxidant activity with IC50 value of 13.79 μg/mL and iron reduction with FRAP value of 387.5±6.41 µM/g. However, a high correlation was observed between the antioxidant and the total phenolic content (r2 = 0.970 - 0.974) but little correlation in total flavonoid (r2 = 0.345 - 0.373). Furthermore, the EF (19.56±7.35) showed the highest toxicity activity followed by CE (22.42±2.10), HF (39.52±7.38), and EAF (41.75±5.10). Therefore, the paku atai tuber can be potentially developed as a natural antioxidant and anticancer material.

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Characterization and Applications of Leaf Extracts of Hemigraphis Alternate

International Journal of Engineering and Advanced Technology - Regular Issue ◽

10.35940/ijeat.a1020.1291s619 ◽

2019 ◽

Vol 9 (1S6) ◽

pp. 108-114

Keyword(s):

Wound Healing ◽

Crude Extract ◽

Leaf Extract ◽

Cellular Migration ◽

Leaf Extracts ◽

Ic50 Value ◽

The Gift ◽

Healing Property ◽

Low Cytotoxicity ◽

Wound Healing Property

Natural products carry out various capabilities and a lot of them have thrilling and useful organic activities. The gift take a look at turned into undertaken to analyze the bioactive compounds and the pharmacological capacity of leaf extract of Hemigraphis alternata. The presence of numerous compounds become characterized by way of FTIR and GCMS. ROS effect and wound recuperation assets have been analyzed and it discovered that crude extract is greater powerful inhibiting ROS manufacturing and it has high wound healing property with properly cellular migration in dose based way. The cytotoxic nature of leaf extract also analyzed with the aid of MTT assay and it suggests low IC50 value of 329.95 µG/ml. The result discovered that the crude extract of H.Alternata leaf is a great wound healer with low cytotoxicity.

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“Pharmaco-chemical Characterization and Evaluation of In Vitro Antioxidant and Antidiabetic Activity of Ethanolic Flower Extract of Clerodendrum paniculatum”

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i3.2688 ◽

2020 ◽

Vol 11 (3) ◽

pp. 4569-4577

Author(s):

Sincy Varghese ◽

Kanakasabapathi Devaki ◽

Poornima Kannappan ◽

Sri Rashmy Madathil

Keyword(s):

Secondary Metabolites ◽

Plant Material ◽

Quantitative Estimation ◽

Chemical Characterization ◽

Antidiabetic Activity ◽

Flower Extract ◽

Ms Analysis ◽

Ic50 Value ◽

Biochemical Features

Quantitative and qualitative analysis of different components and antioxidant and activities of the extract of flower (CPF) were analyzed in . identification of of flower was also identified by GC-MS analysis. assess the biochemical features of CPF. solvent extraction of CPF was performed using solvents in increasing order of polarity (petroleum ether, chloroform, ethyl acetate, ethanol, and water) and solvent with maximum profile was standardized for further analysis. Quantitative analysis of selected secondary metabolites like tannin, , alkaloids, and of the flower extract was done by UV . In antioxidant assays and in efficacy of the flower extract were analyzed by respective in assays. identification of in CPF was identified by using GC-MS analysis revealed secondary metabolites in the extract, and further analysis of the extract was performed. Quantitative estimation revealed an accountable amount of secondary metabolites like (47.66mg/g acid equivalent), (24mg/g equivalent), (41mg/g equivalent), and alkaloids (1.79mg/g of extracted plant material). analysis (GC-MS) also confirmed convincing compounds in the extract. From in antioxidant and assay, the IC50 value of the extract of CPF was measured and compared with standard, and from the results, it was evident that the extract had significant in antioxidant and activity. the above results, it can be confirmed thatCPF has got significant and therapeutically active ingredients, as evident in analysis. This is further supported by considerable antioxidant and properties observed in respective assays.

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Characterization of Leader Processing Shows That Partially Processed Mersacidin Is Activated by AprE After Export

Frontiers in Microbiology ◽

10.3389/fmicb.2021.765659 ◽

2021 ◽

Vol 12 ◽

Author(s):

Jakob H. Viel ◽

Amanda Y. van Tilburg ◽

Oscar P. Kuipers

Keyword(s):

Antimicrobial Activity ◽

Synthetic Biology ◽

Heterologous Expression ◽

Expression System ◽

Heterologous Expression System ◽

Proteolytic Activation ◽

E Coli ◽

Gram Positive Bacteria ◽

Precursor Peptide ◽

Potential Applications

The ribosomally synthesized and post-translationally modified peptide mersacidin is a class II lanthipeptide with good activity against Gram-positive bacteria. The intramolecular lanthionine rings, that give mersacidin its stability and antimicrobial activity, are specific structures with potential applications in synthetic biology. To add the mersacidin modification enzymes to the synthetic biology toolbox, a heterologous expression system for mersacidin in Escherichia coli has recently been developed. While this system was able to produce fully modified mersacidin precursor peptide that could be activated by Bacillus amyloliquefaciens supernatant and showed that mersacidin was activated in an additional proteolytic step after transportation out of the cell, it lacked a mechanism for clean and straightforward leader processing. Here, the protease responsible for activating mersacidin was identified and heterologously produced in E. coli, improving the previously reported heterologous expression system. By screening multiple proteases, the stringency of proteolytic activity directly next to a very small lanthionine ring is demonstrated, and the full two-step proteolytic activation of mersacidin was elucidated. Additionally, the effect of partial leader processing on diffusion and antimicrobial activity is assessed, shedding light on the function of two-step leader processing.

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