scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN KEMANGI (Ocimum basilicum L.) TERHADAP BAKTERI Streptococcus mutans

2020 ◽  
Vol 2 (2) ◽  
pp. 69-76
Author(s):  
Aminah Nur Syarifuddin ◽  
Rosa Aldora Purba ◽  
Novidawati Boru Situmorang ◽  
Romauli Anna Teresia Marbun
Keyword(s):  
Streptococcus Mutans ◽  
Extraction Method ◽  
Research Method ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Effective Concentration ◽  
Treatment Groups ◽  
Gram Positive Bacteria ◽  
Inhibitory Power ◽  
Growth Of Bacteria

Introduction: Basil leaf (Ocimum basilicum L.) is one of the most accessible natural plant in Asia such as Indonesia. Basil leaf vegetation contains a lot of flavonoid compounds and essential oils that have the effect as antibacterial because it can inhibit the growth of bacteria Streptococcus mutans cause dental caries. Streptococcus mutans is a round gram-positive bacteria that typically form pairs or chains during its growth. The purpose of this research is to know the antibacterial effectivity of ethanol extract of basil leaves (Ocimum basilicum L.) on the growth of Streptococcus mutans bacteria.This research was conducted with laboratory experimental research method. Basil lean ethanol extract was prepared by using maseration extraction method. Method: The method used in the inhibitory test using diffusion of the disc with 5 samples in each treatment group. The sample consisted of 5 treatment groups, ethanol  extract of basil leaves with concentration of 100%, 80%, 60%, 40% and 20%. Result: The result of this study showed that ethanol extract of basil leaves with 100% concentration had inhibitory power of 10,26 mm (strong category), 80% of 9,65 mm (medium category), 60% by 8,12 mm (medium category), 40% by 7,33 mm (medium category), and 20% by 6,90 mm (medium category). Conclusion: The conclusion of this research proves that basil leaf ethanol extract has antibacterial power agains Streptococcus mutans bacteria with effective concentration is 100% with strong category equal to 10,26 mm.

OPTIMAL CONCENTRATION OF BASIL LEAF EXTRACT (Ocimum basilicum L.) IN INHIBITING THE GROWTH OF Streptococcus mutans IN VITRO

2021 ◽  
Vol 17 (2) ◽  
pp. 97-102
Author(s):  
Dwis Syahrul ◽  
◽  
Dwis Syahriel ◽  
Keyword(s):  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Culture Medium ◽  
Ocimum Basilicum ◽  
Optimal Concentration ◽  
Control Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
Inhibitory Power

Introduction: One of flora in the oral cavity is Streptococcus mutans as a cause of dental caries. Various ways can be done to suppress its growth, one of them by using mouthwash which is used contains a lot of chemicals, so an alternative is needed by using herbal ingredients, including basil (Ocimum basilicum L.) leaves that contain essential oils, methyl eugenol, phenols, and flavonoids which are able to work as antibacterial. The purpose of this study was to determine the inhibition and optimal concentration of basil leaf extract on the growth of Streptococcus mutans. Materials and method: The method used agar Kirby Bauer method with seven treatments of leaf extract with concentrations of 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, and methanol solution as control group. The culture medium used was Muller Hinton Blood Agar. Results and discussions: The Kruskall Wallis test showed a significant difference between treatment groups. Mann Whitney U-Test test, found that the control group, basil leaf extract concentrations of 1.5%, 2%, 2.5% and 3% were tested with concentrations of 3.5% and 4% had a significant difference. The test between groups of 3.5% and 4% basil leaf extract did not show a significant difference. Conclusion: this study was that in vitro basil leaf extract with a concentration of 3.5% and 4% had inhibitory power on the growth of Streptococcus mutans and basil leaf extract with a concentration of 3.5% optimally inhibited the growth of Streptococcus mutans in vitro

UJI AKTIVITAS EKSTRAK ETANOL 96% BIJI SRIKAYA (Annona squamosa L.) SEBAGAI ANTIDIARE YANG DISEBABKAN OLEH BAKTERI Shigella dysenteriae DENGAN METODE DIFUSI CAKRAM

2017 ◽  
Vol 1 (2) ◽  
pp. 181
Author(s):  
Leonov Rianto ◽  
Indri Astuti Handayani ◽  
Annisa Septiyani
Keyword(s):  
Statistical Tests ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Negative Control ◽  
Seed Extract ◽  
Shigella Dysenteriae ◽  
Annona Squamosa ◽  
Inhibitory Power ◽  
Growth Of Bacteria ◽  
Positive Controls

This research aims to find out how to extract ethanol 96% seed custard apples as Antidiarrhoeal that is caused by bacteria Shigella dysenteriae with different concentrations and compare with chloramphenicol. In addition, also to find out whether their respective inhibitory power of concentration of ethanol 96% seed extract custard apples have a difference in meaning. Research done by experimental methods, was carried out in the laboratory of pharmaceutical Academy IKIFA. Testing was done using the diffusion disc method. The concentration of ethanol 96% seed extract custard apples are used i.e. 15%, 30%, 45%, 60%, and 75%. As positive controls used chloramphenicol 30 µg/ml and NaCl 0.9% as the negative control. Each test is carried out as many as eight repetitions. The results showed an average diameter drag power which formed on the test solution with a concentration of 15%, 30%, 45%, 60% and 75% respectively of 6,887 mm, 11,49 mm, 8,144 mm, 7,694 mm, 7,150 mm, whereas for chloramphenicol for 40,31 mm. Based on the results of the statistical tests there are meaningful differences between concentrations showed a meaningful difference between the concentration of 15% and 30%, 15% and 60% and 15% and 75%. Conclusion of this research is at a concentration of 15%, 96% ethanol extract of seeds of custard apples have been able to inhibit the growth of bacteria, Shigella dysenteriae

scholarly journals Uji Efek Antibakteri Ekstrak Etanol Kulit Batang Salam {Syzigium polyanthum (Wight) Walp} terhadap Staphylococcus aureus dan Escherichia coli secara Invitro

2015 ◽  
Vol 4 (2) ◽  
Author(s):  
Ikhsan Amanda Putra ◽  
Erly Erly ◽  
Machdawaty Masri
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Descriptive Study ◽  
Effective Concentration ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Activity Test ◽  
Growth Of Bacteria

AbstrakTumbuhan salam (Syzygium polyanthum (Wight) Walp.) telah dikenal sejak dahulu untuk mengobati berbagai penyakit. Bagian tumbuhan yang dapat dimanfaatkan sebagai obat selain daun adalah bagian kulit batang. Beberapa penelitian yang telah dilakukan terbukti bahwa daun salam memiliki efek antibakteri. Tujuan penelitian ini adalah untuk mengetahui bagaimana efek antibakteri dari kulit batang salam. Penelitian ini bersifat deskriptif menggunakan metode difusi agar. Konsentrasi ekstrak yang digunakan adalah 25%, 50%, 75%, dan 100%. Hasil penelitian menunjukkan bahwa ekstrak etanol kulit batang salam memiliki efek antibakteri terhadap Staphylococcus aureus dengan konsentrasi 25%, 50%, 75%, dan 100% memberikan daya hambat terhadap pertumbuhan bakteri sebesar 12 mm, 13,67 mm, 12,33 mm, dan 9 mm, sedangkan pada konsentrasi yang sama untuk Escherichia coli tidak terlihat daya hambat terhadap pertumbuhan bakteri. Konsentrasi ekstrak yang paling efektif dalam menghambat S. aureus adalah konsentrasi 50%, dimana konsentrasi 75% dan 100% kurang efektif.Kata kunci: uji efek antibakteri, kulit batang salam, staphylococcus aureus, eschericia coli AbstractSalam plants (Syzygium polyanthum (Wight) Walp.) Salam plants have been known since ancient to treat various diseases. The parts of the plant that can be used as drug are bark. From the research that has been conducted has proven that Salam leaves has an antibacterial effect. The objective of this study was to determine the antibacterial effect of salam bark.This was a descriptive study by using agar diffusion method. The concentration of the extract used was 25%, 50%, 75%, and 100%. The results showed that the ethanol extract of Salam bark has antibacterial effects to Staphylococcus aureus with the concentration of 25%, 50%, 75%, and 100% gives the inhibition of the growth of bacteria on 12 mm, 13.67 mm, 12.33 mm, and the 9 mm, while at the same concentration for Escherichia coli was no bacterial inhibition area. The most effective concentration of extract in inhibiting S. aureus was concentration of 50%, while the concentration of 75% and 100% less effective.Keywords: antibacterial activity test, salam bark, staphylococcus aureus, eschericia coli

scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BATANG DAN PELEPAH DAUN TANAMAN PISANG AMBON ( Musa paradisiaca var sapientum (L.) Kunt ) TERHADAP BAKTERI Staphlyococcus aureus

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 571
Author(s):  
Claudia L. Adilang ◽  
Nancy Pelealu ◽  
Gayatri Citraningtyas
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Extraction Method ◽  
Native Plants ◽  
Ethanol Extract ◽  
Growth Inhibitors ◽  
Clear Zone ◽  
Banana Plant ◽  
Musa Paradisiaca ◽  
Growth Of Bacteria

Banana is one of the Indonesia native plants which containing acid compounds such as hydroxynnamik, flavanones, flavonols, dopamine and N-Acetylserotonin. Banana plant has many benefits, one of which for herbal medicine. The purpose of this study was to test effect of ethanol extract from the stem and sheathof banana as growth inhibitors of Staphylococcus aureus bacteria and to evaluate, eitherstem or sheath parts of Staphylococcus aureus bacteria. The extraction method of this study using maceration with 96% ethanol. Based on the results obtained, the ethanol extract of banana steams was batter in inhibits the growth of bacteria of Staphylococcus aureus with the average of clear zone was 10.00 mm (10%), 17.00 mm (30%), 19.00 mm (50%), 19.33 mm (70%), and 20.33 mm (90%), respectively. Keywords : Musa paradisiaca var sapientum, Antibacterial activity, Staphylococcus aureus. ABSTRAKPisang merupakan salah satu tanaman asli Indonesia yang mengandung senyawa asam hidrosinamik, flavonoid, flavonol, dopamine dan N-asetilserotonin. Bagian dari tanaman pisang memiliki banyak manfaat yang salah satunya untuk obat herbal. Tujuan penelitian ini adalah untuk menguji pengaruh ekstrak etanol dari pelepah dan batang pisang ambon sebagai penghambat pertumbuhan bakteri Staphlyococcus Aureus dan untuk melihat bagian mana dari pelepah atau batang tanaman pisang yang lebih baik dalam menghambat pertumbuhan bakteri Staphlyococcus aureus. Metode ekstraksi dari penelitian ini menggunakan maserasi dengan pelarut etanol 96%. Ekstrak yang diperoleh diuji aktivitas antibakterinya. Berdasarkan hasil yang diperoleh ekstrak etanol batang tanaman pisang lebih baik dalam menghambat pertumbuhan dari bakteri Staphlyococcus aureus dengan rata-rata zona bening kosentrasi 10% (10,00 mm), 30% ( 17,00 mm ), 50% ( 19,00 mm ), 70% ( 19,33 mm ), dan 90% ( 20,33 mm ). Kata Kunci : Musa paradisiaca var sapientum (L.) Kunt, aktivitas antibakteri, Staphlyococcus aureus.

scholarly journals The inhibitory effectiveness of ethanol extract of selasih leaves towards Streptococcus sanguis growth

2015 ◽  
Vol 27 (1) ◽  
Author(s):  
Ivhatry Rizky Octavia Putri Susilo ◽  
Mieke Hemiawati Satari ◽  
Ame Suciati Setiawan
Keyword(s):  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Antibacterial Effects ◽  
Inhibitory Zone ◽  
Volatile Oils ◽  
Gram Positive Bacteria ◽  
Streptococcus Sanguis ◽  
Experimental Laboratory ◽  
Active Content

Introduction: Selasih is one of tropical and subtropical plant used as traditional medicine. It has antibacterial activity especially toward Gram-positive bacteria because it contains volatile oils, tannin, flavonoid and tertepenoid. Method: The study was performed as an experimental laboratory study used Kirby Bauer agar diffusion applied to eight samples of Streptococcus sanguis which was done by twice repetition for each sample. The concentrations of ethanol extract were 16%, 8%, 4%, 2% and 1%.The result was analysed using ANOVA method for single factor of experimental design. The result of the study showed that there was evidence of the antibacterial effect contained in the ethanol extract of selasih leaves inhibiting the growth of Streptococcus sanguis . The average of inhibitory zone for each concentration in every sample, were: 5,25 mm for 16%, 3,22 mm for 8%, 1,84 mm for 4%, 1,53 mm for  2% and 0,19 mm for 1%. The conclusion of the study indicates that there is an antibacterial effects in ethanol extract of selasih leaves (Ocimum basilicum Linn) which inhibits the growth of Streptococcus sanguis. This antibacterial strength is caused by the active content of selasih leaves extract.Conclusion: The extract of basilicum ethanol (Ocimum basilicum L) effectively inhibits the growth of Streptococcus sanguis.

scholarly journals Efektivitas Ekstrak Serbuk Batang Siwak Terhadap Pertumbuhan Bakteri Streptococcus Pyogenes

2020 ◽  
Vol 18 (1) ◽  
pp. 100
Author(s):  
NUGROHO EKO WIRAWAN BUDIANTO
Keyword(s):  
Streptococcus Mutans ◽  
Streptococcus Pyogenes ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Streptococcus Faecalis ◽  
Group Design ◽  
Post Test ◽  
Stem Extract ◽  
Inhibitory Power ◽  
Test Control

<p>Abstract</p><p><strong>Background</strong> : Siwak (Salvadora Persica)  is a plant that is widely used in the Middle East which was used to clean teeth and mouth. Currently there is also toothpaste that contains siwak as its active ingredient. Previous research, Siwak has anti-bacterial power against Streptococcus mutans and Streptococcus faecalis. This study aims to prove whether the extract of siwak stem powder can be used to inhibit the growth of Streptococcus pyogenes bacteria, one of which can cause pharyngitis.</p><p><strong>Method:</strong> Post Test Control Only Group Design method. 24 samples divided into four replications and six treatments. The research used siwak stem extract at a concentration of 25%, 50%, 75% and 100%</p><p><strong>Result</strong>: showed that the inhibition zone of siwak stem extract on the growth of Streptococus pyogenes obtained an average inhibition zone of 25%, 50%, 75%, and 100% concentrations, namely 0 mm, 8.47 mm, 9.77 mm and 10.37 mm. The statistical test results showed that the diameter of the bacterial inhibition zone in the K1 group was significantly different against K3, K4, K5, K6, as well as between the K2 groups against K3, K4, K5, K6. There is no significant difference between K1 and K2 and K3 with K4 and K4 with K5. At a concentration of 50% the ethanol extract of the siwak stem powder was able to provide an inhibitory power on the growth of Streptococcus pyogenes bacteria and an increase in the concentration of 75% and 100%, the more its inhibition power was increased. At a concentration of 100% it was statistically significant different from the inhibition of amoxicillin</p><p><strong>Conclusion</strong> : Concentration of 50% ethanol extract of siwak stem powder provides minimal inhibitory power on the growth of Streptococcus pyogenes bacteria. The higher the extract concentration, the wider the inhibition zone formed.</p><p><strong>Keywords</strong>:  Sebuk siwak stem, Streptococus pyogenes</p><p><strong> </strong></p><p><strong>Abstrak</strong></p><p><strong>Latar belakang : </strong>Siwak merupakan tumbuhan yang banyak di daerah timur tengah yang dulu dipakai untuk membersihkan gigi dan mulut. Saat ini juga tersedia pasta gigi yang mengandung siwak sebagai bahan aktifnya. Penelitian sebelumnya siwak memiliki daya anti bakteri terhadap streptococcus mutans dan streptococcus faecalis. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak serbuk batang siwak apakah dapat dipakai untuk menghambat pertumbuhan bakteri streptococcus pyogenes yang salah satunya bisa menyebabkan faringitis.</p><p><strong>Metode :</strong> Post Test Control Only Group Design. 24 sampel dibagi menjadi empat kali pengulangan dan enam perlakuan. Penelitian menggunakan ekstrak batang siwak pada konsentrasi 25%, 50%,75% dan 100%.</p><p><strong>Hasil Penelitian :</strong> adanya zona hambat ekstrak serbuk batang siwak terhadap pertumbuhan Streptococus pyogenes diperoleh rata-rata zona hambat dari masing-masing konsentrasi 25%,50%, 75%, dan 100% adalah 0 mm, 8,47 mm, 9,77 mm dan 10,37 mm. Hasil uji statistik menunjukkan diameter zona hambat bakteri pada kelompok K1 berbeda signifikan terhadap K3, K4, K5, K6, serta antara kelompok K2 terhadap K3, K4, K5, K6. Tidak ada perbedaan yang signifikan antara K1 dengan K2 dan K3 dengan K4 dan K4 dengan K5. Pada konsentrasi 50% ekstrak etanol serbuk batang siwak sudah dapat memberikan daya hambat pada pertumbuhan bakteri Streptococcus pyogenes dan peningkatan konsentrasi 75% dan 100%  semakin meningkat daya hambatnya. Pada konsentrasi 100% secara statistik berbeda signifikan dengan daya hambat amoxicillin.</p><p><strong>Kesimpulan :</strong> Konsentrasi 50% ekstrak etanol serbuk batang siwak memberikan daya hambat minimal pada pertumbuhan bakteri Streptococcus pyogenes. Semakin tinggi konsentrasi ekstrak maka zona hambat yang terbentuk akan semakin luas.</p><p><strong>Kata Kunci :</strong> Sebuk batang siwak, Streptococus pyogenes</p>

scholarly journals EFEK UJI DAYA BUNUH EKSTRAK KULIT BUAH JERUK NIPIS (CITRUS AURANTI FOLIA) TERHADAP BAKTERI STREPTOCOCCUS MUTANS

2019 ◽  
Vol 5 (1) ◽  
pp. 30-37
Author(s):  
Miftha Ulya ◽  
Fauzia Nilam Orienty ◽  
Maulida Hayati
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Positive Control ◽  
Effective Concentration ◽  
Citrus Fruits ◽  
Citrus Aurantifolia ◽  
Power Test ◽  
A Value ◽  
Inhibitory Power ◽  
Lime Peel

One effort to reduce the number of dental caries in the community is needed antibacterial that can kill the bacteria Streptococcus mutans. One of the antibacterials of nature that can be used as a drug is lime (Citrus aurantifolia). The purpose of this research was to determine the effect of kill power test extract of lime peel (Citrus aurantifolia) against Streptococcus mutans bacteria, the type of research used is laboratory experimental. The samples were colonies of Streptococcus mutans bacteria obtained from University of Indonesia, with 6 treatments in each extract 12.5%, 25%, 50% and 100%. This study used 4 concentrations (12.5%, 25%, 50% and 100% as well as negative and positive control) using Kruskall-Wallis test. The results showed that there was inhibitory power and kill power extract of citrus fruits (Citrus aurantifolia) against Streptococcus mutans bacteria with a value of 0.000 <0,05. 50% concentration is the most effective concentration for bacterial inhibition and 100% concentration is the most effective concentration for killing strength of Streptococcus mutans bacteria

scholarly journals Perbandingan Aktivitas Antibakteri Ekstrak Bunga dan Daun Beluntas (Pluchea indica (L.) less.) Terhadap Bakteri Staphylococcus aureus

2021 ◽  
Vol 1 ◽  
pp. 547-553
Author(s):  
Hida Ilyana ◽  
Dwi Bagus Pambudi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Extraction Method ◽  
Research Method ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Zone Of Inhibition ◽  
Clear Zone ◽  
Disc Diffusion Method

AbstractChemical constituents contained in the beluntas plant, including alkaloids, essential oils, flavonoids, phenols and tannins have the ability to inhibit the growth of Staphylococcus aureus bacteria. This research uses maceration extraction method. Thick extracts of flowers and leaves were used with a series of extract concentrations. The purpose of this study was to determine differences in the effectiveness of the ethanol extract of the leaves and flowers of beluntas against the growth of Staphylococcus aureus bacteria. The research method used in this study is the disc diffusion method. Data was collected by measuring the diameter of the clear zone formed in each treatment. Extract concentrations used 5%, 7% and 9%. The extracts of flowers and leaves of beluntas (Pluchea indica (L.) Less) have antibacterial activity with a larger zone of inhibition in extracts of leaves of beluntas compared to extracts of leaves of beluntas.Keywords: beluntas : flowers; leaves; extract; antibacterial AbstrakKandungan kimia yang terdapat di dalam tanaman beluntas, diantaranya alkaloid, minyak atsiri, flavonoid, fenol dan tanin memiliki kemampuan untuk menghambat pertumbuhan bakteri Staphylococcus aureus.Penelitian ini menggunakan metode ekstraksi maserasi.Ekstrak kental bunga dan daundigunakan dengan seri konsentrasi ekstrak. Tujuan dari penelitian ini yaitu mengetahui perbedaan efektivitas aktivitas ekstrak etanol daun dan bunga beluntas terhadap pertumbuhan bakteri Staphylococcus aureus. Metode penelitian yang digunakan dalam penelitian ini yaitu metode difusi cakram disk. Pengumpulan data dilakukan dengan cara mengukur diameter zona bening yang terbentuk pada setiap perlakuan. Konsentrasi ekstrak digunakan 5%, 7% dan 9%.Ekstrak bunga dan daun beluntas (Pluchea indica (L.)Less) memiliki aktivitas sebagai anti bakteri dengan zona hambat yang lebih besar pada ekstrak daun beluntas dibandingkan dengan ekstrak daun beluntas.Kata kunci: Beluntas; bunga; daun; ekstrak; antibakteri

scholarly journals ANTIBACTERIAL EFFECT OF JAVA TURMERIC ETHANOL EXTRACT AGAINST DUAL-SPECIES STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SANGUINIS BIOFILM (IN VITRO)

2017 ◽  
Vol 10 (17) ◽  
pp. 57
Author(s):  
Ajrina Busri ◽  
Ria Puspitawati ◽  
Sri Utami
Keyword(s):  
Streptococcus Mutans ◽  
Antibacterial Effect ◽  
Ethanol Extract ◽  
Single Species ◽  
Minimal Bactericidal Concentration ◽  
Streptococcus Sanguinis ◽  
Cell Numbers ◽  
Polymerase Chain ◽  
Growth Of Bacteria

Objectives: The minimal bactericidal concentration (MBC) of Java turmeric (Curcuma xanthorrhiza Roxb.) ethanol extract is 25% against Streptococcus mutans and 15% against Streptococcus sanguinis as single species. This study aimed to examine the antibacterial effect of Java turmeric ethanol extract against S. mutans and S. sanguinis as dual-species. S. mutans and S. sanguinis compete against each other to obtain nutrients.Methods: The antibacterial effect of Java turmeric ethanol extract against dual-species Streptococcus in vitro was analyzed by measuring the growth of bacteria after exposure to the extract by counting colony formation and quantifying bacterial cell numbers using real-time polymerase chain reaction.Result: The MBC of Java turmeric ethanol extract against dual-species Streptococcus is 10%. S. sanguinis is more sensitive to the extract than S. mutans. Conclusions: The antibacterial effect of Java turmeric ethanol extract on S. mutans and S. sanguinis as single species differs from the effect on the bacteria as dual-species of Streptococcus.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL TEMU KUNCI (Boesenbergia pandurata Roxb) TERHADAP BAKTERI Streptococcus mutans

2017 ◽  
Vol 22 (1) ◽  
pp. 59 ◽  
Author(s):  
Fitri Lestari Mahmudah ◽  
Sri Atun
Keyword(s):  
Antibacterial Activity ◽  
Streptococcus Mutans ◽  
Maximum Concentration ◽  
Research Method ◽  
Ethanol Extract ◽  
Positive Control ◽  
Activity Test ◽  
The Subject

Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri dari ekstrak etanol rimpang temukunci (Boesenbergia pandurata Roxb) terhadap bakteri Streptococcus mutans. Subjek penelitian ini adalah ekstrak etanol temukunci. Objek penelitian ini adalah aktivitas antibakteri dari ekstrak etanol temukunci. Metode penelitian ini menggunakan metode difusi dari Kirby-Bauer tes. Sebagai kontrol positif digunakan kloramfenikol. Variasi konsentrasi yang di uji pada kisaran 0,5 sampai 500 µg/ml. Hasil penelitian menunjukkan bahwa ekstrak etanol temukunci bersifat menghambat pertumbuhan bakteri Streptococcus mutans. Konsentrasi maksimal yang dapat menghambat pertumbuhan bakteri Streptococcus mutans yaitu pada konsentrasi 50 µg/ml.  (ANTIBACTERIAL ACTIVITY TEST OF ETHANOL EXTRACT TEMU KUNCI (Boesenbergia pandurata) AGAINST Streptococcus mutans BACTERIA)The purpose of this study was determined the antibacterial activity on ethanol extract of temukunci (Boesenbergia pandurata Roxb) against Streptococcus mutans bacteria. The subject of this research was the ethanol extract of temukunci. The object of this study was the antibacterial activity of the ethanol extract of temukunci. The research method was disk difussion method by Kirby-Bauer test. The positive control that  used was chloramphenicol. The concentration variations were 0.5 sampai 500 µg/ml. The results show that the ethanol extract of temukunci can inhibit the growth of Streptococcus mutans bacteria. Maximum concentration that can inhibit the growth of Streptococcus mutans bacteria is at concentration 50 µg/ml.  

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