Anthocyanin-Colored Microencapsulation Effects on Survival Rate of Lactobacillus rhamnosus GG, Color Stability, and Sensory Parameters in Strawberry Nectar Model

Probiotic microencapsulation is a promising way to produce functional food, while their stability and sensory acceptability still a challenge. This study aims to enhance the functional properties of strawberry (Fragaria × ananassa, cultivar Camarosa) nectar and sensory acceptance using novel anthocyanin-colored microencapsulation of Lactobacillus rhamnosus. Four formulations (F1–F4) of coated materials (alginate, whey protein, and pullulan) integrated with anthocyanin pigment were used for encapsulation. The physical properties of microencapsulated probiotics (size, color, efficiency, stability, and survival rate) and quality parameters of nectar (pH, anthocyanin, and sensory acceptability) during 4 weeks of storage at 4 and 25 °C were evaluated. All formulations exhibited high encapsulation efficiency (> 89%), medium bead size (406–504 μm), and proper color (red color). The microencapsulated cells were stable in simulated gastrointestinal and processing conditions (up 7 log10 CFU mL−1) compared to free cells. F4 (alginate 2% + anthocyanin 0.1% + whey protein 2% + pullulan 2% + cocoa butter 1% + L. rhamnosus GG) showed the greatest viability in nectar during storage (6.72 log10 CFU mL−1/4 °C/4 weeks), while a significant decrease in pH (< 2) and anthocyanin (< 60 mg 100 g−1) was observed in nectar-containing free cells. The sensory scores with a difference-preference test as exploratory and preliminary responses revealed that colored probiotic microcapsules enhanced the sensory characters (up to 4 weeks) and commercially accepted (> 80% agreed) of strawberry nectar. Results demonstrated that anthocyanin-colored alginate-whey protein-pullulan matrix had the potential to enhance probiotic viability in functional nectar without negative impact.

The Effect of Sol-Gel Coatings on the Phosphorus (P) Adsorption Capacity of Calcareous Materials for Use in Water Treatment

(1) Phosphorus (P) removal has proven difficult in decentralized wastewater treatment systems, and P binding material, installed as an external filter, has been proposed for improving P removal. Especially, calcium (Ca)-rich materials have shown promising results. (2) Five calcareous materials were tested with isotherm batch experiments. The material with the highest P adsorption capacity was selected to undergo different Sol-Gel coatings, i.e., different coating dilution ratios (1:10, 1:5, and 1:1) and exposure periods (5, 10, and 15 min). The seven coated materials were evaluated by isotherm experiments. (3) The maximum adsorption capacity (Qmax) was determined by fitting the Langmuir equation. Qmax for the non-coated materials, and ranged from 0.7 (sand) to 35.1 (Catsan) mg P g−1 DW, while the coated materials ranged from 7.8 to 24.7 mg P g−1 DW depending on the coating. Based on the rotated Principal Component Analysis, the most important parameters for Qmax were the texture and the Ca content. (4) Catsan was the most promising material, but when performing a Sol-Gel coating, a trade-off between preserving Qmax and the coating thickness were evident, as the materials with the thinner coating preserved more of the sorption capacity. The development of P binding materials constitutes a useful technology in decentralized wastewater treatment systems.

A mesoscopic analysis of a localized shear band propagation effect on the deformation and fracture of coated materials

The numerical simulations of the deformation and fracture in an iron boride coating – steel substrate composition are presented. The dynamic boundary-value problem is solved numerically by the finite-difference method. A complex geometry of the borided coating – steel substrate interface is taken into account explicitly. To simulate the mechanical behavior of the steel substrate, use is made of an isotropic strain hardening model including a relation for shear band propagation. Local regions of bulk tension are shown to arise near the interface even under simple uniaxial compression of the composition and in so doing they determine the mesoscale mechanisms of fracture. The interrelation between plastic deformation in the steel substrate and cracking of the borided coating is studied. Stages of shear band front propagation attributable to the interface complex geometry have been revealed. The coating cracking pattern, location of the fracture onset regions and the total crack length are found to depend on the front velocity in the steel substrate.

Methods—On the Reliability of the Electrochemical Data Recorded on Nickel Foam in Alkaline Solution: The Illusive Surface Oxide Layer

The extensive application of nickel foam (Ni foam) as current collector in supercapacitors has raised caveats on the contribution of the redox-active Ni foam to the measured capacities. However, due to the overlooked qualitative features (i.e., shapes) of the cyclic voltammograms (CVs), the redox reaction of the Ni foam oxide layer (NiFOL) has been frequently confused with the true electrochemical signature of the coated materials in alkaline solution. Herein, experimental CVs, scanning electron microscopy images, and estimations reveal that due to the high porosity of the Ni foam and its surface reactivity in alkaline solution (1-6 M KOH), the redox peak couple of the NiFOL can potentially be confused with or lead to misinterpretation of the true electrochemical features of the coatings. A classification of previous papers on a group of metal oxides investigated as battery-type or pseudocapacitive electrodes in the positive potential window is also presented to reveal the confusion between NiFOL and the coating when operated in alkaline solution.

Comparative Tribological Study of NiTi Diffusion Coated Titanium with Pure Titanium

Dry tribological behaviors of commercial pure (Cp) titanium and Cp titanium diffusion coated with equiatomic NiTi intermetallic layer were studied and compared at room temperature. Wear tests were performed by a pin on disk tribometer using 52100 steel pins, under various normal loads of 10, 20, and 40 N. worn surfaces were examined by scanning electron microscope, equipped with EDS analyzer. The wear rates of the coated materials were lower than those of the Cp titanium at all loads. This was mainly attributed to the higher hardness of the NiTi intermetallic layer compared to that of the untreated titanium. Furthermore, under an applying load of 10 N, a tribological layer was formed which could protect the surface from severe wear. The results also demonstrated a lower coefficient of friction in the treated specimens compared to those of the Cp materials.

Performance of Sprayed PVDF-Al2O3 Composite Coating for Industrial and Civil Applications

Because of their great water repellency, Superhydrophobic coatings have a major impact on a variety of industrial applications. The current study’s key originality is the development of low-cost, stable, superhydrophobic, and corrosion-resistant composite coatings. In the present work, polyvinylidene fluoride (PVDF)/Al2O3 composite coatings were produced using the spray technique to investigate the wettability and corrosion behavior of the coated materials for industrial and civil applications. PVDF was mixed with various concentrations of Al2O3 nanoparticles, and the mixture was sprayed onto steel, aluminum, and glass substrates. The wettability and morphology of the coated surfaces were investigated using the sessile droplet method and scanning electron microscopy, respectively. The corrosion resistance of bare substrates was compared to that of those coated with PVDF alone and those coated with PVDF/Al2O3 nanoparticles using Tafel polarization techniques. The force of adhesion between the coat and the substrates was measured in pounds per square inch. A nanoindentation test was also used to measure the hardness of the coating layer. The PVDF/Al2O3 coated steel showed a significantly higher water contact angle and lower contact angle hysteresis, reaching 157 ± 2° and 7 ± 1°, respectively, compared to the coated aluminum and glass substrates. Corrosion test results showed that the superhydrophobic PVDF/Al2O3 coatings had a much higher corrosion protection efficiency for steel and aluminum than that of the PVDF ones. The PVDF/Al2O3 coated substrates showed moderate but still acceptable adhesion between the coating layer and the substrates. Moreover, the PVDF/Al2O3 coatings had much better mechanical properties than the PVDF only coatings. Such type of coating could be a promising candidate for possible industrial and civil applications.

A Recombinant Fusion Construct between Human Serum Albumin and NTPDase CD39 Allows Anti-Inflammatory and Anti-Thrombotic Coating of Medical Devices

Medical devices directly exposed to blood are commonly used to treat cardiovascular diseases. However, these devices are associated with inflammatory reactions leading to delayed healing, rejection of foreign material or device-associated thrombus formation. We developed a novel recombinant fusion protein as a new biocompatible coating strategy for medical devices with direct blood contact. We genetically fused human serum albumin (HSA) with ectonucleoside triphosphate diphosphohydrolase-1 (CD39), a promising anti-thrombotic and anti-inflammatory drug candidate. The HSA–CD39 fusion protein is highly functional in degrading ATP and ADP, major pro-inflammatory reagents and platelet agonists. Their enzymatic properties result in the generation of AMP, which is further degraded by CD73 to adenosine, an anti-inflammatory and anti-platelet reagent. HSA–CD39 is functional after lyophilisation, coating and storage of coated materials for up to 8 weeks. HSA–CD39 coating shows promising and stable functionality even after sterilisation and does not hinder endothelialisation of primary human endothelial cells. It shows a high level of haemocompatibility and diminished blood cell adhesion when coated on nitinol stents or polyvinylchloride tubes. In conclusion, we developed a new recombinant fusion protein combining HSA and CD39, and demonstrated that it has potential to reduce thrombotic and inflammatory complications often associated with medical devices directly exposed to blood.

Toward Universal Photodynamic Coatings for Infection Control

The dual threats posed by the COVID-19 pandemic and hospital-acquired infections (HAIs) have emphasized the urgent need for self-disinfecting materials for infection control. Despite their highly potent antimicrobial activity, the adoption of photoactive materials to reduce infection transmission in hospitals and related healthcare facilities has been severely hampered by the lack of scalable and cost-effective manufacturing, in which case high-volume production methods for fabricating aPDI-based materials are needed. To address this issue here, we examined the antimicrobial efficacy of a simple bicomponent spray coating composed of the commercially-available UV-photocrosslinkable polymer N-methyl-4(4'-formyl-styryl)pyridinium methosulfate acetal poly(vinyl alcohol) (SbQ-PVA) and one of three aPDI photosensitizers (PSs): zinc-tetra(4-N-methylpyridyl)porphine (ZnTMPyP4+), methylene blue (MB), and Rose Bengal (RB). We applied these photodynamic coatings, collectively termed SbQ-PVA/PS, to a variety of commercially available materials. Scanning electron microscopy (SEM) and time-of-flight secondary ion mass spectrometry (ToF-SIMS) confirmed the successful application of the coatings, while inductively coupled plasma-optical emission spectroscopy (ICP-OES) revealed a photosensitizer loading of 0.09-0.78 nmol PS/mg material. The antimicrobial efficacy of the coated materials was evaluated against methicillin-susceptible Staphylococcus aureus ATCC-29213 and human coronavirus strain HCoV-229E. Upon illumination with visible light (60 min, 400-700 nm, 65 ± 5 mW/cm2), the coated materials inactivated S. aureus by 97-99.999% and HCoV-229E by 92-99.999%, depending on the material and PS employed. Photobleaching studies employing HCoV-229E demonstrated detection limit inactivation (99.999%) even after exposure for 4 weeks to indoor ambient room lighting. Taken together, these results demonstrate the potential for photodynamic SbQ-PVA/PS coatings to be universally applied to a wide range of materials for effectively reducing pathogen transmission.

Randomly Dispersed Coated Composites Study by Statistical Approach and Numerical Homogenization Method

The aim of this work is the computation of effective elastic properties of 3D 3-phase random heterogeneous coated materials. For that, a new expression of the integral range for 3-phase random coated heterogeneous materials is used. The computation is achieved using a representative microstructure with non-overlapping inclusions. Numerical simulations is used under periodic boundary conditions (PBC) and kinematic uniform boundary conditions (KUBC) prescribed over Representative Volume Element (RVE). The obtained effective elastic properties are compared with different analytical models as Hashin and Shtrikman bounds and the n+1 phase model. Using the statistical methods, a new extension of the integral range for 3-phase coated materials is proposed.

Novel modified QAC antimicrobial coatings: application, evaluation and functional determination

A series of novel quaternary ammonium compounds (QACs) previously synthesized by the Foucher Research Group containing the core motif R1-(CH2)3-N+ (CH3)2-(CH2)3-R2, where R1 represents functional groups responsible for anchoring the compound to various substrates and R2 represents moieties responsible for altering the activity and surface properties of coated materials are described. R1 groups include benzophenone (-O-C6H4-C(O)-C6H5) 1a-11a for anchoring to polymer surfaces, and organosilane (-Si(OCH3) 3b-10b for anchoring to textiles and fabrics, while R2 groups include linear alkyl chains (-(CH2)n-CH3; n = 11, 17) 1-2 and aryl or alkyl sulfonamide containing moieties (-NH-SO2-CXHY, x = 2-10; y = 5-11) 3-11. These compounds were tested for antimicrobial activity at solid/air interfaces (LDI) and were found highly effective against representative Gram-positive and Gram-negative bacteria (except 7a-8a, 11a). Selective solid/liquid antimicrobial testing (LRI) was performed on 1a, 5a and only 5a was found to be highly effective against Gram-positive bacteria.