scholarly journals KARAKTERISTIK KIMIA DAN MIKROBIOLOGI SEDIAAN OPAQUE SOAP DENGAN PENAMBAHAN EKSTRAK ETANOL TEMBAKAU

2021 ◽  
Vol 15 (01) ◽  
pp. 1
Author(s):  
Andi Eko Wiyono ◽  
Herlina Herlina ◽  
Andrew Setiawan Rusdianto ◽  
Mega Desy Safitri
Keyword(s):  
Descriptive Analysis ◽  
Inhibition Zone ◽  
National Standards ◽  
The Public ◽  
E Coli ◽  
Randomized Design ◽  
Microbiological Characteristics ◽  
Completely Randomized Design ◽  
Toxic Alkaloids ◽  
Tobacco Extract

Opaque soap is a solid soap product. It was made from a mixture of oil and the addition of alkaline compounds to form a saponification process. Solid soap preparations made with the addition of tobacco extract as an active ingredient that contains nicotine compounds, which include toxic alkaloids are weakly basic. In addition, tobacco extracts chosen to produce products that were beneficial to society. The purpose of this study was to provided wider use of tobacco and to know the characteristic of opaque soap preparations with the addition of tobacco extracts. The research method was a single completely randomized design (CRD) with descriptive analysis. In this study, the formulas of opaque soap were A0 (no addition of tobacco extract), A1 (1.2 g of tobacco extract), A2 (2.4 g of tobacco extract), and A3 (4.8 g of tobacco extract). The observed parameters were chemical and microbiological characteristics. All variations of opaque soaps had a pH of 9.51-9.77, water content of 13.15%-14.35%, free alkali 0.056-0.0584, antibacterial (E. coli inhibition zone proved by clear zone) about 8.60-21.50 mm; and nicotine levels 0-383.67 mg/100g. From the results due to chemical and microbiological characteristics, all of the opaque soap with additional tobacco extract was in accordance with the Indonesian National Standards (SNI 3532-2016) so that soap was safe for use by the public. Keywords: chemistry, microbiology, soap preparations, tobacco extract

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK LAMUN (Cymodocea rotundata) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli (Antibacterial Activities of Seagrass Extracts (Cymodocea rotundata) Against Staphylococcus aureus and Escherichia coli)

2017 ◽  
Vol 13 (1) ◽  
pp. 1
Author(s):  
Septiani Septiani ◽  
Eko Nurcahya Dewi ◽  
Ima Wijayanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
E Coli ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Cymodocea Rotundata

Cymodocea rotundata  merupakan salah satu jenis lamun yang berpotensi sebagai antibakteri. Senyawa bioaktif yang bersifat sebagai antibakteri adalah fenol, flavonoid dan tanin. Tujuan penelitian ini adalah untuk mengetahui perbedaan lama inkubasi dan konsentrasi ekstrak lamun C. rotundata yang berbeda terhadap aktivitas antibakteri S. aureus dan E. Coli. Metode penelitian yang digunakan yaitu experimental laboratories dengan menggunakan rancangan dasar penelitian Rancangan Acak Lengkap (RAL) dengan pola faktorial, pola terbagi oleh faktor lama inkubasi bakteri (24 jam, 48 jam dan 72 jam) dan perbedaan konsentrasi ekstrak lamun (5%, 10% dan 15%). Data dianalisis menggunakan SIDIK RAGAM dan dilakukan analisis lanjut Beda Nyata Jujur (BNJ), apabila terdapat perbedaan pada perlakuan. Ekstrak lamun C. rotundata efektif sebagai antibakteri dengan kategori sedang yaitu zona hambat berkisar antara 5-10 mm. Konsentrasi optimum untuk menghambat S.aureus dan E.coli adalah 15% dengan lama inkubasi 48 jam dengan zona hambat yang dihasilkan masing-masing sebesar 6, 123 mm. Dan 5, 833 mm.Cymodocea rotundata is a type of seagrass that has a potential as an antibacterial. Bioactive compounds which act as such as antibacterial phenols, flavonoids and tannins. The purpose of this study was to determine the effect of different concentration and time of incubation  of seagrass C.rotundata as antibacterial against  S. aureus and E. coli. The method used was experimental laboratories using the basic design of the study completely randomized design (CRD) with factorial pattern (2 factor). The first factor were different concentration (5%, 10% and 15% ) and second factor were time of incubations (24 hours, 48 hours and 72 hours). Data were analyzed using ANOVA and conducted a further test Honestly Significant Difference (HSD), if treatment gave significant effect. C. rotundata extracts had antibacterial activity with medium category which inhibition zone ranges from 5-10 mm. The optimum concentration for inhibiting S. aureus and e. coli were 15% with incubation time of 48 hours resulting inhibition zone 6,123 mm and 5,833 respectively.

scholarly journals Pengaruh Uji Antibakteri Ekstrak Rimpang Jahe Terhadap Pertumbuhan Staphylococcus Aureus Dan Escherichia Coli Secara In Vitro

2020 ◽  
Vol 1 (2) ◽  
pp. 71-80
Author(s):  
Siti Zamilatul Azkiyah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacterial Growth ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
Randomized Design ◽  
Ginger Rhizome ◽  
Completely Randomized Design

The problem of resistance is increasing along with the need for antimicrobials as an alternative in overcoming resistance problems. One solution is to utilize the content of secondary metabolite compounds in rhizome plants such as ginger. The content of compounds that are usually the most dominant in ginger such as essential oils, flavonoids, terpenoids and phenols. This study aims to determine the effect of ginger rhizome extract as an antibacterial on the growth of Staphylococcus aureus and Escherichia coli in vitro. This study was a laboratory experimental study with a completely randomized design (CRD) consisting of 7 treatments with 3 replications. The results showed that the ethanol extract of ginger rhizome had antibacterial activity against S. aureus and E. coli. At concentrations of 20%, 40% and 80% which are more effective in inhibiting the growth of these two bacteria. The higher the extract concentration level, the larger the diameter of the inhibition zone from bacterial growth. This inhibition of bacterial growth is thought to have an effect on the content of the ginger root.   Keywords: Ginger rhizome, Staphylococcus aureus, Escherichia coli, antibacterial ABSTRAK   Permasalahan tentang resistensi semakin meningkat seiring dengan kebutuhan antimikroba sebagai alternatif dalam mengatasi masalah resistensi. Salah satu solusinya yaitu dengan memanfaatkan kandungan dari senyawa metabolit sekunder pada tanaman rimpang-rimpangan seperti rimpang jahe. Kandungan senyawa yang biasanya paling dominan pada jahe seperti minyak atsiri, flavonoid, terpenoid dan fenol. Penelitian ini bertujuan untuk mengetahui pengaruh dari ekstrak rimpang jahe sebagai antbakteri terhadap pertumbuhan Staphylococcus aureus dan Escherichia coli secara in vitro. Penelitian ini termasuk penelitian eksperimental laboratorium dengan rancangan acak lengkap (RAL) yang terdiri dari 7 perlakuan dengan 3 kali ulangan. Hasil penelitian diperoleh bahwa ekstrak etanol rimpang jahe memiliki aktivitas antibakteri terhadap S. Aureus dan E. coli. Pada konsenrasi 20%, 40% dan 80% yang lebih efktif dalam menghambat pertumbuhan kedua bakteri tersebut. Semakin tinggi tingkatan konsentrasi ekstrak maka diameter zona hambat dari pertumbuhan bakteri juga semakin besar. Terhambatnya pertumbuhan bakteri ini diduga adanya pengaruh dari kandungan pada rimpang jahe.   Kata Kunci: Rimpang Jahe, Staphylococcus aureus, Escherichia coli, antibakteri

Herba Reudeuep With Modification of Heating In Virgin Cocanut Oil

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Wahyuzan Wahyuzan ◽  
Lukmanul Hakim ◽  
Rahmat Afrizal ◽  
Asmeri Lamona ◽  
Khairuni Khairuni ◽  
...  
Keyword(s):  
Modern Medicine ◽  
Heating Time ◽  
Agricultural Product ◽  
Average Yield ◽  
Herbal Products ◽  
The Public ◽  
Randomized Design ◽  
Herbal Plants ◽  
Medicinal Properties ◽  
Completely Randomized Design

Traditional medicine in Indonesia has been known by the public before formal health services using modern medicine. Indonesia has many type of herbal plants which have medicinal properties including dadap (Erythrina orientalis). This study aims to utilized dadap herbal liniment by modifying heating in Vigin Cocanut Oil. The study conducted in the laboratory of Agricultural Product Processing using a Factorial Completely Randomized Design (CRD). The first factor is the addition of 250 ml (M1), 375 ml (m2), and 400 ml (M3) Virgin Cocanut Oil treatment. The second factor is the heating tim of 5 minutes (L1), 10 minutes (L2), and 15 minutes (L3). Observation variable consisted of yield, Specific gravity, and organoleptic test of aroma and colored. The result showed an average yield of 87.70-91.88%. The highest yield was found in the treatment of adding 400 ml Virgin Cocanut Oil and 15 minutes of heating time. The highest type of weight was found in the treatment of addition of 325 ml Virgin Cocanut Oil and 5 minutes heating time. Based on the organoleptic scent test, the everage penalist gives a like answer. Organoleptic colors of reudeuep herbal products is according to SNI number 06-23-2006.

PENGARUH SUBSTITUSI TEPUNG BUAH LINDUR (Bruguiera Gymnorrhiza) TERHADAP KARAKTERISTIK ORGANOLEPTIK, NILAI GIZI DAN AKTVITAS ANTIOKSIDAN BROWNIES

2021 ◽  
Vol 6 (3) ◽  
Author(s):  
Wd Wd Sitti Aisyah Nurul Fahlani ◽  
Tamrin Tamrin ◽  
Kobajashi Togo Isamu
Keyword(s):  
Antioxidant Activity ◽  
Nutritional Value ◽  
National Standards ◽  
Bruguiera Gymnorrhiza ◽  
Nutritional Values ◽  
Organoleptic Characteristics ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Range Test ◽  
Color Texture

ABSTRACT                     This study aimed to analyze the effect of lindur fruit flour substitution on organoleptic characteristics, nutritional value, and antioxidant activity of lindur fruit flour brownies. This research was conducted using a completely randomized design (CRD) with various percentages of formulation of substitution of lindur fruit flour:wheat flour, namely M1 = (100%:0%), M2 = (90%:10%), M3 = (80%:20 %), M4 = (70%:30%), and M5 = (60%:40%). The research data were analyzed using analysis of variance, with further testing using Duncan's Multiple Range Test (DMRT) at a 95% confidence level. The results show that the HCN content of lindur fruit flour reached 21.81 mg/kg. The M3 treatment was the most preferred treatment by the panelists with hedonic rating scores of color, texture, aroma, and taste reached 4.14 (like), 4.13 (like), 3.79 (like), and 4.30 (like), respectively. The nutritional values of selected M3 brownies show that it contained 30.57% water, 1.20% ash, 3.70% fat, 5.63% protein, and 58.90% carbohydrate. Analysis of antioxidant activity in selected M3 brownies was 465.58 ppm which is categorized as very weak. It can be concluded that the substitution of lindur fruit flour in making brownies had a very significant effect on color, texture, and taste. However, the effect was not significant on the aroma and the analysis of the nutritional value of the selected treatment. The brownie product substituted with lindur fruit flour met the national standards for water, ash, and fat contents.Keyword: Brownies, Lindur Fruit ABSTRAK    Penelitian ini bertujuan untuk mempelajari pengaruh substitusi tepung buah lindur terhadap karakteristik organoleptik, nilai gizi, dan aktivitas antioksidan brownies tepung buah lindur. Penelitian ini dilakukan dengan menggunakan Rancangan Acak  Lengkap  (RAL) dengan berbagai presentase formulasi, substitusi tepung buah lindur: tepung terigu yaitu M1 =  (100% : 0%), M2 = (90% : 10%), M3 = 80% : 20%), M4 = (70% : 30%), dan M5 = (60% : 40%). Data hasil penelitian dianalisis menggunakan sidik ragam (Analysis of Varian), dengan uji lanjut menggunakan Duncan’s Multiple Range Test (DMRT) pada taraf kepercayaan 95%. Hasil penelitian menunjukan analisis kadar HCN tepung buah lindur yaitu 21,81 mg/kg. Perlakuan M3 merupakan perlakuan yang paling disukai oleh panelis dengan skor penilain hedonik warna 4,14 (suka), tekstur  4,13 (suka), aroma 3,79 (suka) dan rasa 4,30 (suka). Analisis Nilai gizi produk browies tepilih M3 meliputi kadar air (30,57%), kadar abu (1,20), Kadar lemak (3,70%), kadar protein (5,63%) dan kadar karbohidrat (58,90%). Analisis aktivitas antioksidan pada brownies terplih M3 yaitu 465,58 ppm dengan kategori sangat lemah. Dapat disimpulkan bahwa substiusi tepung buah lindur pada pembuatan brownies berpengaruh sangat nyata terhadap warna, tekstur, dan rasa. Tetapi berpengaruh tidak nyata terhadap aroma. dan analisis nilai gizi perlakuan terpilih. Berdasarkan standar mutu SNI brownies, bahwa produk brownies substitusi tepung buah lindur sudah memenuhi standar mutu SNI untuk kadar air dan kadar abu serta kadar lemak.Kata kunci:   Brownies, tepung, Buah Lindur

scholarly journals Sensitivity Testing of Ciplukan (Physalis angulata) Rod Crude Extract on The Bacterium Pseudomonas fluorescens In Vitro

2019 ◽  
Vol 10 (1) ◽  
pp. 24-30
Author(s):  
Ion Tarsardo Sianturi ◽  
Arief Prajitno ◽  
Ellana Sanoesi
Keyword(s):  
Experimental Method ◽  
Crude Extract ◽  
Inhibition Zone ◽  
Natural Material ◽  
Sensitivity Testing ◽  
Randomized Design ◽  
Physalis Angulata ◽  
Completely Randomized Design

Diseases is a problem in aquaculture, one of which is the diseases caused by the bacteriumP.fluorescens. Antibiotics and chemicals materials themselves can give the bacterium resistance and cause harm for the environment. One alternative that can be done is using a natural material, namely the rod of ciplukan (p. angulata). The purpose of this research was to explain the effect of P. angulata on the growth of p. fluorescens. The method which can be use is experimental method by using the research design of completely randomized design (ral) with 5 treatment and 2 control with 3 repetitions. The results showed that extract of P. angulata at various dose (6,67 ppt, 13,33 ppt, 19,99 ppt, 26,66 ppt and 33,33 ppt) exhibited anti-P. fluorescens activity with inhibition zone diameters in the range of (4,06±0,07 mm - 9,63±1,61 mm). Increashing extract dose lead to increased the inhibition zone. The extract dose of 33,33 ppt exhibited best anti-P. fluorescens activity in this research. The research results show that P. angulata is proven to be able to inhibit the P. fluorescens, but to prove the effectiveness of this material, an in vivo is required.

scholarly journals UJI FITOKIMIA DAN DAYA HAMBAT EKSTRAK DAUN JUWET (Syzygium cumini) TERHADAP PERTUMBUHAN Escherichia coli DAN Staphylococcus aureus ATCC

2017 ◽  
Vol 5 (2) ◽  
pp. 47 ◽  
Author(s):  
Kadek Sudarmi ◽  
Ida Bagus Gede Darmayasa ◽  
I Ketut Muksin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Syzygium Cumini ◽  
E Coli ◽  
Randomized Design ◽  
Test Concentration ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Test Level

Bacterial Escherichia coli dan Staphylococcus aureus ATCCis a normal flora bacteria that naturally exist human body. This bacteria may by pathogenic if it exceeds acertain limits. To prevent the disease caused, need to be overcome by using herbal ingredients one of them is juwet leaves (Syzygiumcumini).The purpose of the study was to determine wheter the extract of juwet leaf (S. cumini) can inhibited the growth of E. coli and S. aureus ATCC and to know compounds contained in juwet leaf extract (S. cumini). This research used diffusion wells methodwith 6 treatments and 4 replications. Date analysis using completely randomized design (CRD) with using ANOVA variance analysis. If the date obtained has a real difference test level at the 5% (P? 0,5) that continue with Duncan test. Concentration of the extract tested was 0%, 5%, 10%, 15%, 25% and 50%. Juwet leaf extract is able to inhibited the growth of E. coli and S. aureus ATCC which is shown by the formation of clear zone. Concentration extract leaf which is effective to inhibit E. coli and S. aureus ATCC is the concentration 50% (18,9 mm) for E. coli and (16,5 mm) for S. aureus ATCC. Screening of phytochemical test of juwet leaf extract positive containing alkaloids, phenolic, steroids and saponins.   Keywords : juwet leaf extract (Syzygium cumini), Escherichia coli, Staphylococcus aureus ATCC  

scholarly journals Effects of alphamune G on the performance, serum and haematological parameters of Escherichia coli challenged turkey poults

2020 ◽  
Vol 40 (2) ◽  
pp. 112-121
Author(s):  
S. A. Bolu ◽  
M. T. Adelakun
Keyword(s):  
Escherichia Coli ◽  
Poor Performance ◽  
Positive Control ◽  
Negative Control ◽  
E Coli ◽  
Turkey Poults ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Improved Performance ◽  
Haematological Indices

A study was conducted to determine the response of Turkey poults to graded levels of Alphamune G (0.00+, 0.03, 0.04, 0.05, 0.06 and 0.00 %-) when challenged with Escherichia coli orally for 7 days. The graded levels were the treatments viz 0.00%+ (positive control), Alphamune G at 0.03, 0.04, 0.05, 0.06% and 0.00%- (negative control; infected without Alphamune G supplementation). Each treatment was allotted 3 replicates of 6 poults. The experiment which was conducted for 56 days employed a completely randomized design. E. coli was isolated from the intestinal digesta of a colisepticaemic chicken. 108 turkey poults were used in this study. Poults were infected with E.coli for 7 days through the drinking water and given the treatment. The performance parameters of Alphamune G supplementation were significantly affected. The cumulative weight, Feed intake and weight gain were highest for turkey poults fed 0.06% Alphamune G supplementation. These values were also directly proportional to the supplementation levels of Alphamune G. The birds given the negative treatment (0.00 %-) had relatively poor performance compared to the other treatments. The specific enzymes studied were significantly affected (p<0.05) by the treatments. ALT and AST were significantly highest for turkey poults fed the negative control. Enzyme values became optimum at 0.05% Alphamune G supplementation. At 0.06% of Alphamune G supplementation, cellular mitigations of the effects of E. coli was measurable. Urea and creatinine were not significantly (p>0.05) influenced by the treatments. Haematological indices such as WBC and specific differential counts (lymphocytes and neutrophils) were affected significantly (P<0.05) by supplemental levels of Alphamune G The Inclusion of Alphamune G at 0.06% in the diets improved performance of turkey poults when challenged with Escherichia coli.

scholarly journals +6282294617836

2019 ◽  
Vol 6 (3) ◽  
Author(s):  
Dora Villa Manik ◽  
Ma'ruf Tafsin ◽  
Armyn Hakim Daulay ◽  
Nurzainah Ginting ◽  
Hasnudi
Keyword(s):  
Broiler Chickens ◽  
Body Weight Gain ◽  
Animal Husbandry ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Study Program ◽  
E Coli ◽  
Feed Cost ◽  
Randomized Design ◽  
Completely Randomized Design

DORA VILLA MANIK, 2018. "Effectiveness of Gambir Solution (UncariagambirRoxb) on the Performances of Broiler Chickens Infected by Escherichia coli".Supervised by MA'RUF TAFSIN and ARMYN HAKIM DAULAY. Gambir has the potential as an antibacterial which is traditionally used as a drug for diarrhea. This study examines the effectiveness of gambir solution on performance which includes feed consumption, body weight gain, feed conversion and income over feed cost (IOFC) in broiler chickens infected with E. coli. It was held in November - December 2017 at the Laboratory of Biology, Animal Husbandry Study Program, Faculty of Agriculture University of Sumatera Utara, using a completely randomized design (CRD) with 6 treatments and 3 replications. The treatment consisted of P0A = without infection and without treatment, P0B = infection with E. coli and P1, P2, P3, respectively E. coli infection + Gambir solution 2%, 4%, 6% and P4 = E. coli + infection Tetracycline antibiotics. The results showed that the administration of gambir solution at a dose of 2% - 6% was significantly (P<0.05) effective in improving the performances of broiler chickens infected with E.coli and able to compensate for the use of commercial antibiotics in the form of tetracycline. The highest IOFC is in the 4% gambir solution.

scholarly journals Pengaruh pH terhadap Produksi Antibakteri oleh Bakteri Asam Laktat dari Usus Itik

2017 ◽  
Vol 15 (3) ◽  
Author(s):  
Rudy Sutrisna ◽  
Christina Nugroho Ekowati ◽  
Edelina Sinaga Sinaga
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Analysis Of Variance ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Bacterial Isolates ◽  
Clear Zone ◽  
Effect Of Ph ◽  
Randomized Design ◽  
Completely Randomized Design

This study aimed to determine the effect of pH on the production of antibacterial by lactic acid bacteria. This study uses a completely randomized design factorial 3X 5 (3 X 5 treatment of bacterial isolates pH) with three replications. The first factor consisted of pH 4, pH 5, pH 6, pH 7, pH 8, and the second factor that isolates B2, B7, B8, whereas the parameters consists of a clear zone. The amount of production is determined by the size of the diameter of antibacterial inhibition zone against Salmonella sp. Differences in the production of antibacterial determined based on the results of analysis of variance. The treatment significantly further test BNT at 5% level. The results showed that the effect of media pH test based on the potential for the production of antibacterial effect. The diameter of the largest clear zone produced by B7 isolates at pH 6 was 17.2 mm, 17.1 mm B8 and B2 at pH 7 at 16.7 mm. The smallest diameter of the clear zone produced by B8 at pH 4 was 15.1 mm, B2 at pH 6 there is a clear zone of 12.7 mm and isolate B7 at pH 5 of 11.5 mm Keywords: Antibacterial, Lactic Acid Bacteria, pH, Salmonella sp, Clear zone

scholarly journals EFEKTIVITAS EKSTRAK KULIT BATANG MAJA (Aegle marmelos L) TERHADAP KEMATIAN LARVA NYAMUK Anopheles sp.

2021 ◽  
Vol 15 (2) ◽  
pp. 67
Author(s):  
Sinta Debi Pratama ◽  
Prayudhy Yushananta
Keyword(s):  
Contact Time ◽  
Animal Health ◽  
Work Productivity ◽  
Bark Extract ◽  
Mosquito Larvae ◽  
Malaria Endemic Area ◽  
Aegle Marmelos ◽  
The Public ◽  
Randomized Design ◽  
Completely Randomized Design

<em>Malaria is one of the public health problems that can cause death in infants, children under five, pregnant women and reduces work productivity. Vector control by chemical means leads to vector resistance and environmental pollution and negatively impacts public and animal health. This study aims to determine the effectiveness of the bark extract of Maja (Aegle marmelos L) against the death of Anopheles sp mosquito larvae with concentrations of 5%, 10%, 20%, 40%, and 50% and the observation of contact times every 15 minutes, 30 minutes, 60 minutes, 90 minutes and 120 minutes. The study used a Completely Randomized Design (CRD) with Factorial. Extraction used maceration method, with 70% ethanol as solvent. Larvae of Anopheles sp. instar III obtained from malaria endemic area. The test used a 200 ml container, with two replications. All data were analyzed by two way ANOVA and Tukey HSD test. This study obtained mortality of 93.3% larvae at a concentration of 50% and a contact time of 120 minutes. The results of statistical analysis showed a significant effect of concentration (p=0.0001), contact time (p=0.0001), and interaction concentration-contact time (p=0.004) on the mortality of Anopheles sp. mosquito larvae. This study has proven the use of bark extract of Maja (Aegle marmelos L) as an alternative bio-insecticide in controlling mosquito larvae of Anopheles sp.</em>

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