scholarly journals EFFECT OF ETHANOL EXTRACT OF PICRIA FEL-TERRAE LOUR. LEAVES ON TRIGLYCERIDE AND CHOLESTEROL LEVELS OF WHITE RATS

2018 ◽  
Vol 11 (13) ◽  
pp. 243
Author(s):  
Urip Harahap ◽  
Marianne Marianne ◽  
Yuandani Yuandani ◽  
Hafiza Mitha Agustya ◽  
Dira Ummul Azizah ◽  
...  
Keyword(s):  
Triglyceride Level ◽  
Ethanol Extract ◽  
Control Group ◽  
Test Animal ◽  
Male And Female ◽  
Cholesterol Levels ◽  
White Rats ◽  
Group Extract ◽  
Different Doses

  Objective: The objective of the study was to evaluate the effect of Picria fel-terrae on triglyceride and cholesterol levels in long-term duration.Methods: The effect of ethanol extract of P. fel-terrae leaves was performed using white rats divided into several groups, which include control group, extract at doses of 125, 250, 500, and 1000 mg/kg body weight. The extract at different doses was orally administered to the test animal for 90 days. Then, the triglyceride and cholesterol levels were measured.Results: The ethanol extract of P. fel-terrae in long-term use did not affect the cholesterol level of male and female white rats. However, there was a significant increased in triglyceride level as compared to normal control (p<0.05).Conclusion: The ethanol extract of P. fel-terrae increased triglyceride level only without increasing the level of cholesterol.

RESEARCH ON THE LONG-TERM EFFECTS OF THE CARBOHYDRATE-VITAMIN-MINERAL CONCENTRATE “LIZUNETS SOLEVIT”

2020 ◽  
Vol 15 (8) ◽  
pp. 1147-1153
Author(s):  
D.D. Khayrullin ◽  
Keyword(s):  
Aqueous Suspension ◽  
Animal Husbandry ◽  
The Body ◽  
Control Group ◽  
Long Term Effects ◽  
Rat Pups ◽  
White Rats ◽  
Production Increase ◽  
Different Doses

The main factors constraining the development of animal husbandry is a weak fodder base, due to insufficient supply of nutrients; the costs of its production increase and becomes the cause of metabolic disorders in animals. The aim of our research was to study the embryotoxic and teratogenic effect of the new CVMC “Lizunets-Solevit” on white rats. For this reason, the drug was administered to pregnant females in the form of an aqueous suspension in different doses for 19 days. By the end of the study, no visible signs of fetal abnormalities were found after autopsy of the experimental rats. There was no significant increase in the number of corpus luteum compared with the control group at 1/10 dose by 1.1% and 1/20 dose by 1.95%, respectively. The preimplantation death of zygotes was taken into account on the embryotoxic effect of the drug, in comparison with the control group at a dose of 1/10 more by 19.7%, and at a dose of 1/20 less by 6.23%. The total embryonic mortality is higher in the experimental groups of animals by 12.69% and 3.74% than in the control. Post-implantation death of embryos in the control group was 4.99%, in the experimental 1/10 dose - 4.69% and in the experimental 1/20 dose - 6.66%. In terms of physiological development, the rat pups of the experimental groups did not differ from the control. Thus, it was found that CVMC “Lizunets-Solevit”, in doses of 1/10 and 1/20, has no embryotoxic and teratogenic effects in the body of white rats, their embryos and young animals of the neonatal and postnatal periods of life.

scholarly journals Hubungan Pemberian Ekstrak Cordyline fruticosa L.A Cheval Terhadap Penurunan Kolesterol Tikus Putih

2020 ◽  
pp. 152
Author(s):  
Dian Rahmawati
Keyword(s):  
Total Cholesterol ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Cholesterol Levels ◽  
White Rats ◽  
Group I ◽  
Significant Difference ◽  
Cordyline Fruticosa

ABSTRACT This study aims to determine the effect of ethanol extract of cordyline fruticose leaves in reducing total cholesterol levels of male white rats. This study used 30 male white rats which were divided into 6 treatment groups, each groups consistent of 5 test animals. Group I was normal control, group II was negative control (High cholesterol died and streptozotocin 30 mg/kgBW, group III was positive control (Simvastatin suspension), group IV, V, VI were each given an ethanol extract of cordyline fruticose leaves at a dose of 100 mg/kgBW, 150 mg/kgBW and 200 mg/kgBW for 14 consecutive days. Measurements of analyzed using a one-way statistical test (ANOVA) at 95% confidence level and continued with the Least Significant Difference (LSD) test to analyze differences between the groups. The result showed that the ethanol extract of cordyline fruticose leaves at a dose 200 mg/kgBW was able to reduce total cholesterol levels in white rats with cholesterol level of 39 mg/dL. Keywords: High fat feed, Cordyline fruticosa, Cholesterol.

scholarly journals HYPOGLYCEMIC AND HYPOLIPIDEMIC EFFECTS OF ANNONA MURICATA L. LEAF ETHANOL EXTRACT

2017 ◽  
Vol 9 (3) ◽  
pp. 170 ◽  
Author(s):  
E. Sovia ◽  
W. Ratwita ◽  
D. Wijayanti ◽  
D. R. Novianty
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ethanol Extract ◽  
Normal Group ◽  
Islet Of Langerhans ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Annona Muricata ◽  
Cholesterol Levels

Objective: The aim of this study was to investigate hypoglycemic and hypolipidemic effects of Annona muricata leaf ethanol extract. This study also investigated phytochemical analysis of the extract and improvement in the islet of Langerhans.Methods: Hypoglycemic and hypolipidemic effects were evaluated using alloxan induced diabetic rats. Twenty-five Wistar rats devided into five groups, that are normal group, control group that induced by alloxan 125 mg/kg body weight and given CMC (carboxymethyl cellulose), and three treatment group that induced by alloxan and given Annona muricata leaf ethanol extract (AMLEE) with 50, 100 and 200 mg/kg body weight. Blood glucose and total cholesterol levels were measured before and after alloxan induction, and 21 d after AMLEE treatment. At the end of the study, all of the animals experiment were sacrificed for histopathological examination.Results: Phytochemical analysis revealed that flavonoids, tannins, saponins, phytosterols and phenols were present in ethanol extract of Annona muricata L. leaf. At the end of the study, blood glucose and total cholesterol levels in all AMLEE treated group were decreased significantly (p<0.05). Final blood glucose level in the groups that given AMLEE 50 mg/kg body weight (86.7±14.6 mg/dl) was almost the same with that of the normal group (91.25±28.38 mg/dl). The result of the histopathological examination is not showed an improvement of the islet of Langerhans in AMLEE treated groups.Conclusion: In conclusion, the ethanol extract of Annona muricata L. leaf have hypoglycemic and hypolipidemic effects. However, there was no improvement in the islet of Langerhans damage.

scholarly journals Biochemical Activity and Hypoglycemic Effects ofRumex obtusifoliusL. Seeds Used in Armenian Traditional Medicine

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Anush Aghajanyan ◽  
Armine Nikoyan ◽  
Armen Trchounian
Keyword(s):  
Herbal Remedy ◽  
Density Lipoprotein ◽  
Ethanol Extract ◽  
Liver Glycogen ◽  
Lipoprotein Cholesterol ◽  
Current Therapy ◽  
Total Phenolic ◽  
Control Group ◽  
Biochemical Activity ◽  
Cholesterol Levels

Diabetes mellitus (DM) is a serious chronic metabolic disorder. Various diseases are being treated with medicinal plants and that is because of the less side effects of the current therapy. The diversity of plants in Armenia is due to the singularity of natural environment. However, biochemical activity of these plants has not been studied well. Thus, the goal was to investigate biochemical activity and antihyperglycemic properties ofRumex obtusifoliusL. in rabbits with hyperglycemia. The high content of total phenolic compounds, flavonoids, and tannins has been determined in this plant extract. Oral administration of ethanol extract showed significant effect on hyperglycemia, reducing fasting glucose levels (57.3%, p<0.05), improving glucose tolerance, and increasing liver glycogen content (1.5-fold, p<0.01) compared to the hyperglycemic control group. Furthermore, ethanol extract ofR. obtusifoliusreduced total cholesterol, low-density lipoprotein cholesterol levels, and vice versa increased high-density lipoprotein cholesterol levels and also decreased liver enzymes levels (alanine aminotransferase and aspartate aminotransferase) compared with untreated group. These findings suggest thatR. obtusifoliusmay have beneficial effects and should be supplement, as herbal remedy in the treatment of DM.

scholarly journals THE EFFECT OF NONI (Morinda citrifolia) EXTRACT TO HEPAR HISTOPATHOLOGY AND TRIGLYCERIDE LEVEL IN RATS (Rattus Norvegicus) INDUCED BY DISLIPIDEMIA

2019 ◽  
Vol 20 (2) ◽  
pp. 76-81
Author(s):  
Jhouharotul Faradisah ◽  
Diah Purwaningsari
Keyword(s):  
Lipid Metabolism ◽  
Triglyceride Level ◽  
Rattus Norvegicus ◽  
Morinda Citrifolia ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
White Rats ◽  
Abnormal Lipid Metabolism

Dyslipidemia is an abnormal lipid metabolism which may cause fat degeneration on hepatocytes cells and elevated triglyceride serum level. Dyslipidemia can be prevented by the consumption of high antioxidant food. Noni(Morinda citrifolia) contains many antioxidant such as flavanoid, kuersetin, tannin, and saponin, which are able to prohibit the elevation of ROS.This research is aimed to find out the effect of noni(Morinda citrifolia) extract in reducing the number of hepatocyte’s cells with fat degeneration and decreasing the triglyceride level which is elevated due to high cholesterol diet induction.In this study white rats divided randomly into 4 groups, control group (K-), high cholesterol diet induced group (K+), high cholesterol diet induced with 100 mg/Kg BW noni extract group (P1), high cholesterol diet induced with 200 mg/Kg BW noni extract group (P2). The result shows that noni  extract with dose 100 mg/Kg BW and  200 mg/Kg BWcan reduce the number of hepatocytes cells with fat degeneration (p= 0,026 and p=0,027) and decrease the level of triglyceride serum (p=0,036 and p=0,010).The conclusion is noni extract with dose 100 mg/KgBW reduces effectively  the number of hepatocyte’s cells with fat degeneration and decreases the level of triglyceride serum which increase because of high cholesterol diet. 

scholarly journals PENGARUH PEMBERIAN INFUSA DAUN LABU SIAM (Sechium edule) TERHADAP PENURUNAN KADAR KOLETEROL DARAH TOTAL TIKUS PUTIH JANTAN (Rattus norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 151
Author(s):  
Ausich Singal ◽  
Edwin De Queljoe ◽  
Paulina Yamlean
Keyword(s):  
Rattus Norvegicus ◽  
Dose Group ◽  
T Test ◽  
Blood Cholesterol ◽  
Control Group ◽  
Cholesterol Levels ◽  
White Rats ◽  
Sechium Edule ◽  
One Way Anova ◽  
Paired T Test

ABSTRACT This study aims to determine the effect of infusion of conjoined pumpkin leaves (Sechium edule) on reducing total blood cholesterol of male white rats (Rattus norvegicus). The subjects of this study were 15 male white rats with an average body weight of 200 grams which were divided into 5 groups, each group consisted of 3 mice. The method used is a laboratory experiment with a completely randomized design. The results were obtained from 2 measurements of blood cholesterol levels, namely measurements before and after treatment. The treatment begins with the provision of high-fat foods for 48 days. On the 49th day a blood cholesterol level was measured before treatment. Furthermore, treatment was given to each group, namely aquades in the negative control group, simvastatin in the positive control group, and squash leaves infusion with their respective doses in the dose group I (40%), the dose group II (20%), and the dose group III (10%). Measurement of cholesterol levels after treatment was carried out on day 54. Data were analyzed by Paired t-test and One Way ANOVA. The analysis showed that there were no significant differences between treatment groups. Judging from the change in average and percentage, 40% infusion dose of siamese pumpkin leaves gives the best reduction in cholesterol levels. Keywords: Cholesterol, pumpkin leaves, male white mouse infusion. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian infusa daun labu siam (Sechium edule ) terhadap penuruan kolesterol darah total tikus putih jantan (Rattus norvegicus). Subjek penelitian ini berupa tikus putih jantan berjumlah 15 ekor dengan berat badan rata-rata 200 gram yang dibagi dalam 5 kelompok, masing-masing kelompok sebanyak 3 ekor. Metode yang digunakan yaitu eksperimen laboratorium dengan rancangan acak lengkap. Hasil penelitian diperoleh dari 2 kali pengukuran kadar koleterol darah yaitu pengukuran sebelum dan sesudah perlakuan. Perlakuan dimulai dengan pemberian makanan tinggi lemak selama 48 hari. Pada hari ke49 dilakukan pengukuran kadar kolesterol darah sebelum perlakuan. Selanjutnya diberikan perlakuan pada tiap kelompok yaitu aquades pada kelompok kontrol negatif, simvastatin pada kelompok kontrol positif, dan infusa daun labu siam dengan dosis masing-masing pada kelompok dosis I (40 %), kelompok dosis II (20 %), dan kelompok dosis III (10%). Pengukuran kadar kolesterol sesudah perlakuan dilakukan pada hari 54. Data diananlisis dengan Paired t-test dan One Way ANOVA. Hasil analisa menunjukan tidak terdapat perbedaan yang bermakna antar kelompok perlakuan. Dilihat dari perubahan rerataan dan presentase, dosis infusa  40% daun labu siam memberikan penurunan kadar koleterol terbaik. Kata kunci : Infusa daun Labu siam, kolesterol, tikus putih jantan.

scholarly journals UJI ANTIDIABETIK EKSTRAK ETANOL BUNGA PEPAYA (Carica papaya L.) TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 160
Author(s):  
Adinda Fransisca Pongoh ◽  
Edwin De Queljoe ◽  
Henki Rotinsulu
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Carica Papaya ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Control Group ◽  
Homogeneity Test ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

ABSTRACT This study aims to determine the antidiabetic activity of papaya flower ethanol extract (Carica papaya L.) against male white rats (Rattus norvegicus) induced by alloxan. This research is experimental. Fifteen rats were divided into 5 treatment groups, each group consisted of 3 rats. The first group was the negative control group given Aquades, the second group was positive control given Glibenklamid, the three groups were 200 mg dose variation groups, four groups were 400 mg dose variations, and the five groups were 800 mg dose variations. Previously, rats were examined fasting blood glucose levels, then mice were induced by an alloxan dose of 120 mg / kgBW intraperitoneally. On the 3rd day blood glucose levels were examined and then treated according to groups for 7 days, measurement of blood glucose levels after the treatment was carried out once every 3 days namely day 3, day 7, and day 10. Data obtained were then analyzed statistically using SPSS , including normality test (Shapiro-Wilk), homogeneity test (Levene), ANOVA test (One way). The results of this study indicate that Papaya Flower (Carica papaya L.) Ethanol Extract can provide the best blood glucose level reduction effect at a dose of 800 mg. Keywords : Antidiabetic, Papaya Flower Extract (Carica papaya L.), Diabettes Mellitus, Male White Rat (Rattus norvegicus), Aloxan.  ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas Antidiabetik Ekstrak Etanol Bunga Pepaya (Carica papaya L.) Terhadap Tikus Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan. Penelitian ini bersifat eksperimental. Sebanyak 15 ekor tikus dibagi ke dalam 5 kelompok perlakuan masing- masing kelompok terdiri dari 3 ekor tikus. Kelompok pertama kelompok Kontrol negatif yang diberikan Aquades, Kelompok kedua Kontrol Positif yang diberikan Glibenklamid, kelompok tiga kelompok variasi dosis 200 mg, kelompok empat kelompok variasi dosis 400 mg, dan kelompok lima kelompok variasi dosis 800 mg. Sebelumnya tikus dilakukan pemeriksaan kadar glukosa darah puasa, selanjutnya tikus diinduksi Aloksan dosis 120 mg/kgBB secara intraperitoneal. Pada hari ke 3 diperiksa kadar Glukosa darah kemudian diberikan perlakuan sesuai kelompok selama 7 hari, pengukuran kadar glukosa darah setelah perlakuan dilakukan 3 hari sekali yaitu hari ke 3, hari ke 7, dan hari ke 10. Data yang diperoleh kemudian dianalisis secara statistik menggunakan SPSS, meliputi uji normalitas (Shapiro-Wilk), uji homogenitas (Levene), uji ANOVA (One way). Hasil penelitian ini menunjukkan bahwa Ekstrak Etanol Bunga Pepaya (Carica papaya L.)  dapat memberikan efek penurunan kadar glukosa darah yang paling baik yaitu pada dosis 800 mg. Kata kunci : Antidiabetik, Ekstrak Bunga pepaya (Carica papaya L.), Diabettes Mellitus, TikusPutih Jantan (Rattus norvegicus), Aloksan.

scholarly journals PENGARUH PEMBERIAN AIR SEDUHAN BERAS YANG DIFERMENTASI OLEH MONASCUS PURPUREUS (ANGKAK) TERHADAP PENURUNAN KADAR KOLESTEROL SERUM PADA TIKUS PUTIH

Biomedika ◽  
2014 ◽  
Vol 6 (1) ◽  
Author(s):  
Adhimass Wicaksono ◽  
Retno Sintowati ◽  
Sa’idatul Fitriyah
Keyword(s):  
Treatment Group ◽  
Serum Cholesterol ◽  
Brown Rice ◽  
Negative Control ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Cholesterol Levels ◽  
White Rats ◽  
Group A

The using of plant-based medicine is a popular approach to health care. A study presented by American Hearth Association showing that brown rice can lower cholesterol level in bood serum. To know the benefi ts of Angkak’s steeping water to decrease serum cholesterol levels in mice and determine the effects of Angkak’s steeping water to decrease serum cholesterol levels in rats compared with simvastatin. This study used an experimental research design, and using pre and post test control group design, performed in the laboratory of biomedical III FK UMS on November 1, 2012 to date of January 1, 2013. The purposive sampling technique, sampel determinaton using formula Federer by the number of sample of 30 male white rats wistar strain. After the data is collected, analyzed using the Shapiro-Wilk test, ANOVA, LSD, and the unpaired t test. The result of this study demnstrate hypothesis testing using ANOVA test with signifi cant level 95% of the negative control group, the treatment group a dose of 1, 2, and 3, it was found p<0,05 were signifi cantly different meaning. In the test dose of LSD between treatment groups 2 and 3 the value of p=0,839 (p>0,05) wich means no signifi cant difference. In the unpaired T test between the treatment group a dose of 1, 2, and 3 with positive control group were signifi cant difference. The unpaired T test between the treatment group a dose of 1, 2, and 3 with white rice also found signifi cant difference. Conclusion: Angkak’s steeping water can lower serum cholesterol levels of white rat as well as the water steepimg Angkak has the same effectiveness with simvastatin to decrease serum cholesterol levels of mice.Keyword: Cholesterol, Angkak

scholarly journals Evaluation of Some Male and Female Rats’ Reproductive Hormones Following Administration of Aspartame With or Without Vitamin C or E

2021 ◽  
Vol 45 (2) ◽  
pp. 14-20
Author(s):  
Omar H Azeez
Keyword(s):  
Vitamin C ◽  
Reproductive Hormones ◽  
Female Rats ◽  
Control Group ◽  
Male And Female ◽  
Group 4 ◽  
Male And Female Rats ◽  
Group 3 ◽  
Group 2

Aspartame (ASP) is a sugar substitute. Its use rose because it has been demonstrated to have deleterious effects after being metabolized. In the presence of antioxidant vitamins C or E, the effects of ASP on reproductive hormones of adult male and female Albino Wister rats were investigated. A total of eighty male and female rats were used in this study. The rats were divided into four groups: group 1, received no treatment; group 2, received ASP at 40 mg/kg BW; group 3, received ASP at 40 mg/kg BW with vitamin C at 150 mg/kg BW; and group 4, received ASP at 40 mg/kg BW and vitamin E at 100 mg/kg BW. All treatments were given orally by gavage needle once daily for consecutive 90 days. The levels of estradiol (E2), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and testosterone hormone (TH) were measured after 90 days in blood plasma. In comparison with the control group, ASP treatment resulted in lower levels of E2, FSH, and LH in male and female rats. When the antioxidants vitamin C or E was given, the effects of ASP were reversed, and the levels of E2, LH, and FSH were increased. The testosterone hormone was likewise significantly increased by ASP, but testosterone hormone concentrations were decreased by vitamin C or E treatments. Long-term ASP consumption caused interfering with testicular and ovarian hormonal activity, while vitamins C and E on the other hand, overcome longstanding consumption ASP's effects.

TOTAL FLAVONOID DAN EFEKTIVITAS EKSTRAK ETANOL BIJI KELOR (Moringa oleifera L) ASAL KOTA PALU SULAWESI TENGAH TERHADAP HISTOPATOLOGI PANKREAS TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI STREPTOZOTOCIN

2020 ◽  
Vol 6 (1) ◽  
pp. 24
Author(s):  
Viani Anggi ◽  
Joni Tandi ◽  
Veronika Veronika
Keyword(s):  
Ethanol Extract ◽  
Negative Control ◽  
Total Flavonoid ◽  
Male Rats ◽  
Control Group ◽  
Β Cells ◽  
Pancreatic Β Cells ◽  
White Rats ◽  
Group I ◽  
Streptozotocin Induced Diabetes

This study aims to determine the content of flavonoid and the effect of ethanol extract of moringa seeds on the regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes. This study method used has total flavonoid equivalent quercetin by spectrophotometry uv-vis and to regeneration of pancreatic β cells in male white rats used 30 test animals,namely male white rats divided into 6 groups, each group consisted of 5 male white rats with details of group I as normal control, Group II as negative control given 0.5% Na-CMC suspension, Group III as positive control given glibenclamide suspension and in Groups IV, V, and VI were given with each dose of 100 mg/kg BW, 200 mg/kg BW and 400 mg/kg BB. Histopathological damage picture of the pancreas was observed by staining HE using a 400x magnification olympus Cx21 microscope. The results showed that the ethanol extract of moringa seeds contained secondary metabolites, namely flavonoids, alkaloids, saponins and tannins. The results showed has total flavonoid equivalent quercetin of moringa seeds is 1,26% and regeneration of pancreatic β cells in male white rats streptozotocin induced diabetes of Moringa seed ethanol extract at a dose of 400 mg/kg BB can have an effect on the regeneration of β cells in the pancreas of white diabetic male rats.  

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