scholarly journals Perbedaan Zona Hambat Terhadap Jamur Malassezia Furfur Antara Pemberian Ekstrak Umbi Bawang Putih (Allium Sativum Linn) Dengan Ekstrak Kulit Umbi Bawang Putih (Allium Sativum Linn)

2018 ◽  
Vol 1 (2) ◽  
pp. 77
Author(s):  
Diah Ariana
Keyword(s):  
Traditional Medicine ◽  
Allium Sativum ◽  
Inhibition Zone ◽  
Malassezia Furfur ◽  
Standard Result ◽  
Garlic Bulb ◽  
Rating Method ◽  
The Difference ◽  
Tinea Versicolor ◽  
Anti Fungal

Tinea versicolor or pitiriasis versicolor is a skin disorder caused by Malassezia furfur infection which is a fungal infection on the skin of the chronic surface. Clinical features is in the form of fine spots that colored white to brown black, this infection is also called a skin fungus with blotches. Treatment of Tinea versicolor can use chemical or traditional medicine. Chemical treatments such as creams or ointments cause sticky taste, while one of the traditional medicine is the bulb and skin of the garlic bulb (Allium sativum Linn) containing allicin substances that are efficacious as an anti-fungal.               This research is an experimental research with the aim to know the difference of inhibition zone to Malessezia furfur fungus. There are 3 treatments with 9 repetitions. The extraction method used is the soxhlet method. Anti-fungal activity test was done by using rating method, Saboroud Dextrose Agar (SDA) media which has been inoculated with suspension of Malassezia furfur with concentration 1.5x108 CFU/ml (Mc Farland standard).               Result of data analysis obtained by ANOVA p = 0,000 < α = 0,05, that there is difference of inhibition zone from treatment of garlic bulb extract and skin of garlic bulb (Allium sativum Linn). It is indicated that there is effect of giving garlic bulb extract (Allium sativum Linn) and garlic bulb skin (Allium sativum Linn) to Malassezia furfur fungus. Further tests were performed to compare mean treatment i.e. HSD test. It was obtained sig (p) 0,016 > α 0,05 which means bulb extract and garlic bulb skin (Allium sativum Linn) have different effect. Keywords: Tinea versicolor, Malassezia furfur, garlic bulb.

scholarly journals Review Artikel: Pengaruh Ekstrak Bawang Putih (Allium Sativum L.) Terhadap Pertumbuhan Jamur Malassezia furfur Penyebab Penyakit Panu (Tinea versicolor)

2021 ◽  
Vol 5 (1) ◽  
pp. 46-53
Author(s):  
Hepni Hepni
Keyword(s):  
Allium Sativum ◽  
Malassezia Furfur ◽  
Purposive Sampling ◽  
Tinea Versicolor

Bawang putih selain dikenal sebagai salah satu bumbu dapur dan dipercaya juga sebagai antibiotik dan antifungi. Khasiat ini disebabkan oleh zat allicin yang kandungannya mampu membunuh jamur dan bakteri. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh perbedaan konsentrasi ekstrak bawang putih terhadap zona hambat pertumbuhan jamur Malassezia furfur penyebab penyakit panu (Tinea versicolor), dengan menggunakan metode studi literatur. Teknik pengambilan sampel yang digunakan yaitu purposive sampling dan memperoleh 2 sampel yaitu berupa jurnal dan Karya Tulis Ilmiah yang membahas tentang pengaruh bawang putih dalam mengatasi penyakit panu. Senyawa yang ada pada bawang putih adalah allicinn yang didapatkan dari ekstrak bawang putih yang mempunyai aktivitas dan daya hambat terhadap jamur Malassezia furfur penyebab penyakit panu. Dengan hasil yang didapatkan semakin tinggi konsentrasi ekstrak bawang putih maka semakin luas zona hambat pertumbuhan jamur penyebab panu.

UJI TOKSISITAS AKUT KAPSUL BAWANG PUTIH LANANG (Allium sativum Linn)

2017 ◽  
Vol 2 (2) ◽  
pp. 179
Author(s):  
Lusia Murtisiwi ◽  
Novena Yety Lindawati
Keyword(s):  
Traditional Medicine ◽  
Allium Sativum ◽  
Microbial Pathogens ◽  
Coliform Bacteria ◽  
Plate Count ◽  
Liver Functions ◽  
Dried Extract ◽  
Time Required ◽  
Mold And Yeast ◽  
And Staphylococcus Aureus

Garlic (Allium sativum Linn) is evidently active against Mycobacterium tuberculosis. Garlic contains allicin that is able to inhibit the bacteria causing tuberculosis. This dried extract garlic is also qualified as content of capsules of traditional medicine, score of Total Plate Count is1.5 (required not over 10); figures of mold and yeast extract of garlic is 4.102 (required not over 104); does not contain microbial pathogens (uncontaminated with coliform bacteria, Escherichia coli, Salmonella sp, and Staphylococcus aureus); and aflatoxin is not detected (required not more than 30 CPM); the average of moisture content is 2% (required notmore than 10%). Capsule of dried extract garlic (Allium sativum Linn) meets pharmaceutical requirements for traditional medicine capsule dosage form, with9 minutes as disintegrating time (required not more than 15 minutes) and meets the uniformity of weight as required for capsule. Results of the research indicates that dried extract of garlic indose above 48.750 mg/ kg body weightto get LD50in garlic, belongs to ‘relatively harmless’. Dried extract garlic in 4 doses variations do not affect the liver functions of mice, so significant clinical toxicity symptoms that happen on entire animals test have not gained yet

scholarly journals IDENTIFIKASI SENYAWA GARLISIN DAN KUERSETIN SEBAGAI SENYAWA ANTIMIKROBA PADA SEDIAAN BASAH DAN KERING BAWANG PUTIH (Allium sativum L.)

2020 ◽  
Vol 8 (2) ◽  
pp. 176
Author(s):  
Fanny Thresia Yunus ◽  
Ari Suwondo ◽  
Martini Martini
Keyword(s):  
Traditional Medicine ◽  
Allium Sativum ◽  
Chemical Compounds ◽  
The Body ◽  
Garlic Extract ◽  
Antimicrobial Compounds ◽  
Vacuum Oven ◽  
Garlic Powder ◽  
Three Samples

Indonesian people have long used garlic as traditional medicine because it contains various chemical compounds that are beneficial to the body. In this study identification of garlicin and kuersetin compounds in garlic as an antibacterial. This study requires 500 grams of garlic each in wet preparations consist of fine garlic and garlic extract, and dry preparation consists of garlic powder. In addition, this research requires 2,1 liters of 96% ethanol. Garlic is dried at 400C with TMI Vacuum Oven and extracted at 250C. Garlic powder is made using Krisbow dry cabinet with a temperature of 1000F and a pressure of 10 Pa. Garlisin content was identified by GCMS Shimadzu TQ8030 and FTIR Perkin Elmer Spectrum Two UATR, while identification of quercetin using Shimadzu HPLC type UFLC. The result shows that the compound garlicin (C6H10S2) was found in the three samples with identical similarity levels. In addition, the three samples showed differences in kuersetin content. The highest kuersetin content was 458.729 ppm garlic extract while the lowest kuersetin content was 81.181 ppm fresh garlic. Both of these compounds have potential as antimicrobial compounds.

Comprehensive GC-MS and FT-IR Profiling of Coleus zeylanicus essential oil and investigation of its anti-fungal and anti - oxidant activities

2021 ◽  
Vol 12 (1) ◽  
pp. 636-642
Author(s):  
Lakshmi M ◽  
Nandagopal S
Keyword(s):  
Antioxidant Activity ◽  
Candida Albicans ◽  
Essential Oil ◽  
Malassezia Furfur ◽  
Fungal Strains ◽  
Anti Oxidant ◽  
Ft Ir ◽  
Dpph Scavenging ◽  
Dpph Scavenging Assay ◽  
Anti Fungal

To evaluate the leaf volatile constituents of essential oil of Coleus zeylanicus and evaluate their anti-oxidant and anti-fungal activity. The Chemical composition of Coleus zeylanicus essential oil was determined using GC-MS and FT-IR analytical techniques. The antioxidant activity was evaluated using DPPH scavenging assay. The anti-fungal effect was tested against two potential pathogenic fungal strains - Candida albicans and Malassezia furfur using agar well diffusion method. The essential oil was profiled by the presence of sesquiterpene hydrocarbons 90.67% of their total composition followed by oxygenated monoterpenes and monoterpene hydrocarbons as 5.3% and 2.1% respectively. The GC-MS results showed 14 compounds from Coleus zeylanicus leaf EO representing 98.07% of the total oil composition. The major component was identified as a-Gurjunene (35.94%), a-bisabolol (10.82%) and G-selinene (4.26%). EO showed remarkable antioxidant activity values of IC50 = 59.78± 3.21µg/ml by DPPH scavenging assay. The essential oil showed interesting anti-fungal effects against two pathogenic fungal strains. The most sensible strains to Coleus zeylanicus EO was Malassezia furfur (32.00±0.50mm) compared to that of Candida albicans (15.00±1.25mm). Hence, Coleus zeylanicus EO has potential application against fungal infection and oxidative stress-related diseases. However, further investigations are necessary to isolate and investigate the action mechanism of these bioactive compounds.

ANTIBACTERIAL EFFECTIVENESS OF LIME LEAVES (Citrus hystrix DC) ETHANOL EXTRACT ON Enterococcus faecalis BACTERIA

2021 ◽  
Vol 17 (2) ◽  
pp. 89-96
Author(s):  
Ratih Widyasari ◽  
◽  
Willy Hadinata Halim ◽  
Atia Nurul Sidiqa ◽  
Dewa Made Wedagama ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Negative Control ◽  
Endodontic Treatment ◽  
Leaf Extracts ◽  
Herbal Products ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Difference

Introduction: Endodontic treatment failure can be caused by the bacterium E. faecalis, which has a prevalence of up to 90%. E. faecalis is difficult to eradicate because it forms a biofilm to defend itself against antibacterial agents. Lime leaves are one of the many herbal products available in Indonesia (Citrus hystrix DC). Lime leaf extracts include antibacterial components such as essential oils, phenolics, alkaloids, saponins, and tannins. The purpose of this study was to investigate the antibacterial activity of lime leaf extract against E. faecalis bacterium. Material and Methods:The agar diffusion technique was used for four experimental groups, using lime leaf extract at 4% (group 1), 8% (group 2), and 16% (group 3) as the negative control. Each group was repeated six times on Muller Hinton Agar (MHA) medium with a paper disc, and the diameter of the inhibitory zone was measured with a caliper. Results and Discussions: The results revealed that a 4 % concentration of lime leaf extract had an average inhibition zone diameter of 7.31 mm, an 8 % concentration had an average inhibition zone diameter of 8.59 mm, and a 16 % concentration had an average inhibition zone diameter of 11.41 mm. The difference in the inhibition zone was statistically significant (p= 0,000) evaluated using the one-way ANOVA. Conclusion: It can be concluded that lime leaf extract is antibacterial potential against E. faecalis bacteria in the endodontic treatment.

scholarly journals Structural analysis of fungal pathogenicity-related casein kinase α subunit, Cka1, in the human fungal pathogen Cryptococcus neoformans

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Belinda X. Ong ◽  
Youngki Yoo ◽  
Myeong Gil Han ◽  
Jun Bae Park ◽  
Myung Kyung Choi ◽  
...  
Keyword(s):  
Cryptococcus Neoformans ◽  
Pathogenic Fungus ◽  
Kinase Assay ◽  
Α Subunit ◽  
Dynamic Architecture ◽  
Human Fungal Pathogen ◽  
Life Threatening ◽  
The Difference ◽  
Anti Fungal

Abstract CK2α is a constitutively active and highly conserved serine/threonine protein kinase that is involved in the regulation of key cellular metabolic pathways and associated with a variety of tumours and cancers. The most well-known CK2α inhibitor is the human clinical trial candidate CX-4945, which has recently shown to exhibit not only anti-cancer, but also anti-fungal properties. This prompted us to work on the CK2α orthologue, Cka1, from the pathogenic fungus Cryptococcus neoformans, which causes life-threatening systemic cryptococcosis and meningoencephalitis mainly in immunocompromised individuals. At present, treatment of cryptococcosis remains a challenge due to limited anti-cryptococcal therapeutic strategies. Hence, expanding therapeutic options for the treatment of the disease is highly clinically relevant. Herein, we report the structures of Cka1-AMPPNP-Mg2+ (2.40 Å) and Cka1-CX-4945 (2.09 Å). Structural comparisons of Cka1-AMPPNP-Mg2+ with other orthologues revealed the dynamic architecture of the N-lobe across species. This may explain for the difference in binding affinities and deviations in protein-inhibitor interactions between Cka1-CX-4945 and human CK2α-CX-4945. Supporting it, in vitro kinase assay demonstrated that CX-4945 inhibited human CK2α much more efficiently than Cka1. Our results provide structural insights into the design of more selective inhibitors against Cka1.

Edible film based on Vicia faba shell gum, nano silver and Helichrysum arenarium essence

2019 ◽  
Vol 50 (2) ◽  
pp. 280-287 ◽  
Author(s):  
Farzane Jahantigh
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Natural Antioxidants ◽  
Inhibition Zone ◽  
Edible Film ◽  
Composition Analysis ◽  
Disk Diffusion Test ◽  
Content Type ◽  
The Difference

Purpose The inclusion of natural antioxidants and nanoparticles into edible coatings can improve their structure, functionality and applicability in foods. This study aims to determine the antibacterial effect of new nano-composite based on bean pod shell gum (BPSG), silver nanoparticle (SNP) and herbal essential oil (EO). Design/methodology/approach For this purpose, BPSG (4 per cent w/v), SNP (0.5-1 per cent w/v) and Helichrysum arenarium EO (1-2 per cent v/v) were mixed. After preparation the edible film, the antibacterial activity was tested on five food-borne pathogenic bacteria in two categories including two-gram positives and three-gram negatives bacteria. The EO composition was determined by GC–mass spectrometry and the antibacterial activity was tested using disk diffusion test. Findings Results showed that gram-positive bacteria were more susceptible than gram-negative bacteria. Increasing Helichrysum arenarium EO and SNP content increased antimicrobial activity of the edible film based on BPSG, so that the treatment containing 2 per cent v/v Helichrysum arenarium EO and 1 per cent w/v SNP led to the highest inhibition zone (8.1-13.1 mm) compared to treatment containing 1 per cent v/v Helichrysum arenarium EO and 0.5 per cent w/v SNP with inhibition zone range of 5.4-9.9 mm and the difference was statistically significant (p = 0.41). Also, the chemical composition analysis of the EO identified a total of 38 compounds in which a-pinene (32 per cent), 1,8-cineole (16 per cent), α-humulene (15 per cent) and ß-caryophyllene (8 per cent) were the main fractions. Other separated components accounted less than 29 per cent of the oil. Originality/value In general, SNP and Helichrysum arenarium EO improved the functional properties including the antimicrobial activity of the edible film based on BPSG, which increases the potential to be used as active packaging for fresh products.

scholarly journals In VitroAntioxidant Properties of Three Varieties ofAllium sativumL. Extracts

2010 ◽  
Vol 7 (s1) ◽  
pp. S573-S579 ◽  
Author(s):  
R. T. Narendhirakannan ◽  
K. Rajeswari
Keyword(s):  
Free Radical ◽  
Allium Sativum ◽  
Preventive Intervention ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Garlic Bulb ◽  
Total Antioxidant ◽  
Radical Scavenging Ability ◽  
Dpph Radical Scavenging

Many herbs possess antioxidant ingredients that provide efficacy by additive or synergistic activities.Allium sativumL. is a strong astringent, used for the treatment of liver and spleen diseases, rheumatism and tumors. The antioxidant activities of different concentrations of ethanolic extracts of garlic bulb of three varieties were determined by the four assaysi.e. DPPH radical scavenging assay, reducing power ability, hydrogen peroxide scavenging assay and total antioxidant capacity. Due to its natural origin and potent free-radical scavenging ability,Allium sativumL. could be used as a potential preventive intervention for free radical-mediated diseases.

scholarly journals ANTIFUNGAL ACTIVITY OF RED GALANGAL (Alpinia purpurata K. Schum) ETHANOL EXTRACT AGAINST Malassezia furfur AND Microsporum canis

2020 ◽  
Vol 3 (2) ◽  
pp. 76-81
Author(s):  
Rini Lestari
Keyword(s):  
Antifungal Activity ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Average Concentration ◽  
Diffusion Method ◽  
Microsporum Canis ◽  
Malassezia Furfur ◽  
Agar Diffusion Method ◽  
Alpinia Purpurata ◽  
Anti Fungal

Red galangal (Alpinia purpurata) is a plant that belongs to the Zingiberaceae family. Red galangal rhizome is traditionally used to treat phlegm and ringworm. Red galangal rhizome contains secondary metabolites of essential oils, eugnol, sesquiterpenes, pinen, kaemferida, galanagn and galangol which have anti-fungal properties. This study aims to determine the antifungal activity of red galangal rhizome ethanol extract against Malasezia furfur and Microsporum canis using agar diffusion method. Red galangal extract is made with a concentration variation of 2%, 5% and 10%. The results showed the antifungal activity of red galangal ethanol extract in the form of inhibiting diameters at an average concentration of 2%, 5% and 10% against Malasezia furfur was 16.77 mm, 17.91 mm and 19.72 mm. The average inhibitory diameter of Microsporum canis is 10.58 mm, 13.57 mm and 14.51 mm. Based on the results of this study it can be concluded that the ethanol extract of red galangal has antifungal activity against Malasezia furfur and Microsporum canis.

scholarly journals EFEKTIVITAS GEL EKSTRAK ETANOL BAWANG PUTIH (Allium sativum L.) TERHADAP BAKTERI Staphylococcus aureus

2020 ◽  
Vol 2 (2) ◽  
pp. 105-112
Author(s):  
Irene Puspa Dewi ◽  
Ilham Maslan Orde ◽  
Verawaty Verawaty
Keyword(s):  
Staphylococcus Aureus ◽  
Allium Sativum ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Traditional Medicines ◽  
Significant Difference ◽  
Irritation Test ◽  
Gel Preparation ◽  
The One

Garlic (Allium sativum L.) is one of the traditional medicines which has an antibacterial efficacy compound namely Allisin which is able to inhibit the growth of Staphylococcus aureus bacteria. This study aims to formulate the garlic bulb extract into a good gel preparation for use as an acne drug and to determine the antibacterial effectiveness of the ethanol extract of garlic gel against the bacterium Staphylococcus aureus using the wells method. The gel evaluation was carried out to meet the requirements with organoleptic test parameters, homogeneity, pH, spreadability, adhesion and irritation test. This study uses extracts of 10% and 20% concentrations, at each concentration being able to inhibit the growth of Staphylococcus aureus bacteria. In the formula, the concentration of 20% has a inhibition zone diameter of 1.59 cm and a concentration of 10% has a inhibition of 1.50 cm. Statistical test results using the One Way ANOVA test found that there was no significant difference between the average diameter of inhibition of garlic extract gel concentration of 10% with a concentration of 20%.

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