Virus resistance of potato genetic diversity under climate change

Author(s):  
R.O. Bondus ◽  
Yu.V. Kharchenko ◽  
L. T. Mishchenko

Aim. To determine the resistance of potato gene pool accessions to viruses in the southern forest-steppe of Ukraine and to select starting material for breeding. Results and Discussion. Our analysis of the climate changes for the period of 1955–2018 revealed that the unfavorable weather/climatic conditions during spring planting of tubers, as a result of which weakened plants were more easily depressed by viral infections, were the primary cause of potato cacogenesis in the southern forest-steppe of Ukraine. It was found that significant deviations from the multi-year average temperature and amount of precipitation contributed to the intense mass manifestation of symptoms of viral diseases or under favorable weather/climatic conditions, on the contrary, viral diseases were not visually detected. Studies have shown that the main factor in resistance to viral degeneration of potato varieties is the genotype, and its development and expression depend on growing conditions. It is also important to take into account emergence of new strains of viruses requiring continuous monitoring. Virus resistance of the varieties was visually determined. In order to identify viruses and study their morphology, electron microscopy, EIA and PCR were used. Conclusions. As a result of the study, sources of high field resistance to leaf-rolling, potato rugose and streak mosaics, which are valuable starting material for further practical use in breeding, as well as in scientific and educational programs, were identified.

2018 ◽  
Vol 1 (94) ◽  
pp. 21-26
Author(s):  
M. L. Tyrus

Soil tillage and fertilization level are important in the system of agro-technical measures that increase soil fertility and sugar beets productivity. Today the cultivation of this crop requires a great deal of expense, therefore, there is a need for a wider study of the use of cheap soil cultivation, soil protection, energy-saving soil tillage technologies and appropriate ferti-lization levels. Under western Forest-Steppe of Ukraine conditions, investigations were conducted on dark grey podzolic light loamy soils where the influence of tillage models and fertilization levels on the formation of sugar beets root crops was studied. Shallow no-plow tillage of the soil on 14-16 cm is expedient to use as a main tillage under sugar beets. Under favorable weather and climatic conditions, it can provide yielding capacity at the level of deep fall plowing. The use of shallow no-plow tillage helped to increase the yielding capacity of sugar beet roots relative to plowing by 28-30 cm for 1.3 t / ha and the biological sugar output – for 0.2 t / ha. Then the sugar content was by 0.1% higher in the application of deep fall plowing on 28-30 cm. The optimization of the system of sugar beets fertilization allows ensuring the growth of the yielding capacity of root crops. It is established that the application of fertilizer norm N300P225K350 provides the yielding capacity at the level of 90 t / ha. The sugar content of root crops at the given fertilizer rate was the lowest in the experiment - 16.5 – 16.6%. Thanks to the high yielding capacity of root crops in terms of N300P225K350, the sugar harvesting was the highest and depending on the method of basic soil tillage was 14.95 – 14.79 t / ha. That is in 3.2 times more as to the control, in 1.3 times more than the norm of N180P135K210 and in 1.1 more than the fertilization level N240P180K280.


Author(s):  
N.S. Kozhushko ◽  
M.M. Sakhoshko ◽  
M.G. Bashtovyi ◽  
D.V. Smilyk ◽  
V.I. Avramenko ◽  
...  

According to statistic evaluation of the manifestation of signs of the economic suitability of state sort resources of 2019 registration potatoes when growing it in different agro-climatic conditions, a significant advantage of influence of the forest-steppe conditions was determined. Compared to Polesia the increase of yield rose for 4.1 t/ha or 18 % (НІР05 = 2.18, Ffact= 12.65 > F05 = 4.22) and the amount of trade potato for 22.72 ha or 20 % (Ffact = 58.87). There is also a tendency to increase the number of starch from unit of area for – 17 % and the total consumer properties for – 8 %.  However, there is a significant difference in the reduction of storability of products by 45 % and the lack of it’s resistance to macrosporiosis and late blight. It was proved that the yield of varieties for cultivation in the polesia zone by 25.7 % depended on the duration of the growing season (Ffact = 4.14 > F05  = 0.06). The relationship between yield and starch of potatoes in Polesia zone varieties  was determined – 23.6 % and in forest-steppe – 17.3 % and starch collection, respectively, 33.6 % and 28.0 %. The dependence of storability of potato which grew in the polesia zone and in forest-steppe from the amount of starch for 15 % was mathematically proved (Ffact = 2.11 > F05 = 0.17) і 11.3 % (Ffact = 1.52 > F05 = 0.24); the dependence of storability on the duration of the growing season in the Forest-Steppe conditions was confirmed(Ffact = 0.66 > F05  = 0.43). In addition, the 12 % of tasting grade of the Forest-Steppe varieties depended on the starch content amount (Ffact = 1.65 > F05 = 0.22). According to the results of the regression analysis, mathematical models have been developed to predict yields, culinary and consumer properties, and the storability of new potato varieties under different growing conditions. The project of a conveyor for the consumption of fresh product in summer  period with the involvement of varieties of early ripeness in the Forest-Steppe was presented: 80 days after planting – Sanibel, 90 days after planting – Medison, Paroli, 95 days after planting – Bazalia, Bernina, 100 days after planting – Donata, in the Polesia zone ‒ 80 days after planting – Sanibel, 90 days after planting – Paroli, 95 days after planting –Bazalia, 100 days after planting – Medison, Bernina, Donata. By distribution of varieties by predicted high content of phytonutrients there were bred varieties with red skin and creamy flesh – Bazalia and Sanibel, by high content with yellow flesh – Baltic Rosa and moderate content with white flesh – Oleksandrit, Fotynia, Ricarda. Introduction of new varieties with certain high adaptive potential of storability and content of bioactive compounds in potatoes will provide growth of potato production in the conditions of the northeast forest-steppe and increase its competitiveness in the domestic and foreign markets and will solve the problem of year-round consumption of fresh quality products.


2017 ◽  
pp. 120-127
Author(s):  
S.M. Svyderska

An important element of climate change is to assess changes in agro-climatic growing conditions of crops and the impact of these changes on their performance. Agriculture is the most vulnerable sector of  Ukraine's economy to fluctuations and climate change. Given the inertial nature of agriculture and the dependence of the efficiency on the weather, now need to make timely and adequate solutions to complex problems caused by climate change. Due to the expected increase in air temperature of the Northern Hemisphere food security Ukraine will largely depend on how effectively adapting agriculture to future climate change. This includes advance assessment of the impact of the expected climate change on agro-climatic conditions for growing crops. Potatoes - perennial, herbaceous, plant, but in nature is treated as an annual plant, so that the life cycle, beginning with germination and ending with the formation of bubbles and the formation of mature tubers, is one growing season. Potato is one of the most important crops grown and diversified use in almost all parts of our country. But the main focus areas of potatoes in Polesie and Forest-steppe. We consider the relative performance of the photosynthetic productivity of potato and agro-climatic conditions for growing potatoes for the period 1986 to 2005, and expected their changes calculated by the climate change scenarios A1B and A2 for the period 2011 to 2050 in Eastern and Western Forest-Steppe. We consider the agrometeorological and agro-climatic conditions in which there may be a maximum performance of potato.


2019 ◽  
pp. 25-32
Author(s):  
Julia I. Vargach

Relevance. Each sample is characterized by a number of biological features, due to the recombination of genetic material in the process of its selection. These features are manifested in the reaction of plants to soil-climatic and technological conditions, expressed in the change of the elements of the yield structure and indicators of the level of adaptability of plants to growing conditions, as well as resistance to lodging. New samples have little-known properties that need to be studied and compared in specific soil and climatic conditions, to assess them in order to decide on the possibility of their zoning or culling. The main indicators characterizing the level of agricultural production are productivity, resistance to lodging, since the shortage of grain as a result of lodging of cereals can reach 15-40%. The introduction of new high-yielding varieties on the basis of their testing and the use of the most effective technological methods of cultivation on crops will allow to receive a significant gross harvest.Materials and methods. The study was carried out in 2016-2018 to assess models of cultural hexaploid species of oats hulled and naked forms of resistance to lodging, yield and adaptability in the temperate continental climate of Central non-Chernozem zone. Resistance to lodging, yield variability, indices of environmental conditions and adaptability were determined. The main method of evaluation of the field resistance to lodging – visual (in points) that allows you to rank the varieties based on this characteristic. We were calculated potential productivity and adaptability of varieties by method L. A. Zhivotkov et al. [1], an indicator of the stability level of the sample yield (Puss) by the method of E. D. Nettevich et al. [2].Results. Many varieties showed good plasticity and high productivity potential. The best in resistance to lodging, yield and adaptability among the hulled forms were varieties Sig, Mirt, Poseidon, Belinda, Rajtar, and the naked forms – Becas, Vyatsky, Korolek.


2018 ◽  
Vol 48 (4) ◽  
pp. 56-62
Author(s):  
O. T. Andreeva ◽  
N. G. Pilipenko ◽  
I. P. Sidorova ◽  
N. Yu. Kharchenko

During the research, non-traditional fodder crops that have high fodder productivity and adaptability to natural and climatic conditions of Trans-Baikal Territory were studied. The results of field and laboratory studies for 2014–2016 are presented on the cultivation of annual poaceous fodder crops – feed millet, Sudan grass and barley in single-crop sowings, as well as mixed with high-protein spring rape, in the forest-steppe zone of Trans-Baikal Territory. The research was conducted on meadow-chernozem mealycarbonate soil. Fodder crops were assessed by their adaptability to growing conditions and by a set of economically valuable characteristics. The possibility of increasing productivity and quality of fodder of poaceous agrocenoses by means of cabbage crops was established. Joint sowings of annual fodder crops: feed millet + spring rape, Sudan grass + spring rape provided the maximum collection of green mass – 24.4-25.9 t/ha, dry matter – 3.55-3.78 t/ha, feed units – 3.17-3.30 t/ha, digestible protein – 506-545 kg/ha, gross energy – 37.3-39.3 GJ., with a high supply of digestible protein to a feed unit – 160-165 g/k per unit. Mixed sowings of Sudan grass and feed millet with spring rape increased collection of feed units by 22.3%, digestible protein – by 86.8%, gross energy – by 18.5% compared to single-crop sowings. Barley, when cultivated under the same conditions, was inferior in productivity to feed millet and Sudan grass in single-crop sowings by 1.2-1.7 times, in mixed sowings – by 1.0-1.5 times. 


2020 ◽  
Vol 21 (6) ◽  
pp. 688-696
Author(s):  
E. A. Traburova ◽  
T. A. Rozhmina ◽  
I. A. Andreeva

The research was carried out in 2017-2019 in the conditions of the Smolensk region on sod-podzolic medium loamy slightly acidic soil. The study included 25 varieties and lines of fiber flax of domestic and foreign selection. The mid-ripening variety Impuls (Russia) was used as a standard, whose yield, depending on the test year, was 1.07...1.29 t/ha. As shown by the calculations of the index of environmental conditions, the most favorable conditions for fiber flax developed in 2018 (Ij = +0.46), and stress conditions, caused by excessive moisture during the critical period of plant growth and development (GTC = 1.7), in 2017 (Ij = -0.55). On average, over the years of research, the yield of fiber, depending on the genotype, varied in the range of 1.00...1.97 t/ha. The highest yield on average for three years of testing (1.78...1.97 t/ha) and average yield in contrasting conditions were characteristic of the varieties Cesar, Sinel, Dobrinya (Russia), Marylin (Holland), Andrea, Altea (France). Under favorable weather conditions (2018), a high potential for the yield of flax fiber compared to the average value (121.5...133.3 %) was obtained in the varieties Cesar, Dobrinya, Sinel (Russia), Belita (Belarus), Andrea, Altea (France), Marylin (Holland). Under stressful conditions (2017), high adaptability was found in the samples of the Russian selection Cesar (173.4 %), Sinel (168.0 %), Dobrinya (151.1 %), Tost 3 (128.7 %), Alfa, Universal (127.7 %). The varieties Surskiy (bi = 2.2), Belita (Belarus), l. 323-02 (Russia), Marylin (Holland) − 1.7, Alexandrit (Russia), Andrea (France) – 1.5. Were characterized by high responsiveness to cultivation conditions (bi> 1). Russian varieties with a relatively stable yield: Cesar, Sinel – 0.7, Alpha – 0.6, Lider, Smolich, Tost 3, Universal – 0.5, Impuls – 0,2 did not show reaction to changes in growing conditions (bi <1). Thus, the domestic varieties Cesar, Dobrinya and Sinel have the highest yield of flax fiber and adaptability to the soil and climatic conditions of the Central Non–Black Earth Region. Expansion of fiber flax acreage under these varieties will provide strengthening of the domestic raw material base.


Author(s):  
А.В. Терешкин ◽  
А.Л. Калмыкова ◽  
Т.А. Андрушко

Вертикальное озеленение с участием различных видов лиан в современных условиях имеет важное эстетическое и санитарно-гигиеническое значение. Особо актуально решение вопросов обогащения флоры городских территорий лианами в степных районах в связи с бедным видовым составом и резким ухудшением экологической ситуации. Объектами исследований являлись 7 видов лиан, различных жизненных форм (однолетние, многолетние), произрастающие в населенных пунктах Саратовской области (Аткарск, Саратов). Цель исследований – изучение эколого-биологических особенностей и мелиоративных свойств лиан и выявление перспектив их использования в вертикальном озеленении селитебных территорий Саратовской области. В ходе исследования видового состава, были выявлены наиболее популярные виды однолетних лиан: ипомея красно-голубая (Ipomea tricolor (L.) Roth) и ипомея пурпурная (Ipomea purpurea (L.) Roth), горошек душистый (Lathyrus edoratus L.), настурция (Tropacolum peregrinum L.) и фасоль огненно-красная (Phaseolus coccineus). Большинство (70%) из них произрастают в местах ограниченного пользования. Изученные виды лиан в исследуемых регионах достигают средних природных показателей (при наличии надлежащего ухода), обладают хорошими показателями жизненного состояния, обильно цветут и плодоносят. При воздействии токсикантами различной концентрации на листовые пластинки лиан установлено их степень устойчивости. Выявлено, что однолетние лианы лучше использовать в декоративных целях, а не в санитарно-гигиенических. Сравнительная оценка однолетних видов с многолетними лианами (девичий виноград пятилисточковый и клематис тангутский) показывает устойчивость многолетних видов (в среднем на 3 балла - 40%). Разработаны варианты декоративных композиций с участием травянистых лиан. По степени декоративности выделены однолетние лианы – Ipomea tricolor, I. purpurea (37 – 41 балл), средней степенью отличаются – Lathyrus edoratus (33 балла), Tropacolum peregrinum (30 баллов) и Phaseolus coccineus (20 – 27 баллов). Преимущество многолетних лиан заключается в их устойчивости к резким изменениям климатических условий (на 40 %) по сравнению с однолетними формами. Поэтому они более предпочтительны для озеленения городской среды. Для усиления декоративного эффекта в сезонном аспекте рекомендуются сочетать расширение видового и формового разнообразия растений (многолетние и однолетние лианы, древесно-кустарниковая, цветочная растительность). Установлено, что природно-климатические условия зоны степи и лесостепи в пределах Саратовской области являются достаточно благоприятными для нормального роста и развития древесно-кустарниковой растительности, в том числе травянистых лиан. Таким образом, обоснованное применение древесных лиан в комплексе с традиционными видами насаждений позволит создать комфортные условия проживания населения, регулировать оптимальный температурный баланс и создавать благоприятные микроклиматические условия. Vertical gardening with different types of vines in modern conditions is important aesthetic and sanitary-hygienic value. It is especially important to address the issues of enrichment of the flora of urban areas with vines in the steppe regions due to poor species composition and a sharp deterioration of the ecological situation. The objects of research were 7 species of lianas, various life forms (annual, perennial), growing in the settlements of the Saratov region (Atkarsk, Saratov). The aim of the research is to study the ecological and biological features and reclamation properties of vines and identify the prospects for their use in vertical gardening residential areas of the Saratov region. In the study, species composition was the most popular species of annual vines: morning glory red-blue (Ipomea tricolor (L.) Roth) and purple morning glory (Ipomea purpurea (L.) Roth), the fragrant pea (Lathyrus edoratus L.), nasturtium (Tropacolum peregrinum L.) and runner beans (Phaseolus coccineus). Most (70%) of them grow in restricted areas. Studied species of vines in the study regions reach average natural performance (with proper care), have good indicators of vital condition, bloom abundantly and bear fruit. When exposed to toxicants of different concentrations on the leaf blades of vines established their degree of stability. It was revealed that the annual vines are better used for decorative purposes, and not in the sanitary-hygienic. A comparative assessment of annual species with perennial vines (maiden grapes and clematis Tangut) shows the stability of perennial species (an average of 3 points-40%). The options and decorative compositions with the participation of herbaceous vines. According to the degree of decoration of the allocated annual vine – Ipomea tricolor, I. purpurea (37 – 41 points), the average degree of differ – Lathyrus edoratus (33 points), Tropacolum peregrinum (30 points) and Phaseolus coccineus (20 to 27 points). The advantage of perennial vines is their resistance to sudden changes in climatic conditions (40 %) compared to annual forms. Therefore, they are more preferable for greening the urban environment. To enhance the decorative effect in the seasonal aspect, it is recommended to combine the expansion of species and form diversity of plants (perennial and annual lianas, tree and shrub, floral vegetation). It is established that the climatic conditions of the steppe and forest-steppe zone within the Saratov region are quite favorable for the normal growth and development of tree and shrub vegetation, including herbaceous lianas. Thus, the reasonable use of wood vines in combination with traditional types of plantings will create comfortable living conditions for the population, regulate the optimal temperature balance and create favorable microclimatic conditions.


2018 ◽  
Vol 1 (94) ◽  
pp. 55-61
Author(s):  
R.O. Myalkovsky

Goal. The purpose of the research was to determine the influence of meteorological factors on potato yield in the conditions of the Right Bank Forest-steppe of Ukraine. Methods.Field, analytical and statistical. Results.It was established that among the mid-range varieties Divo stands out with a yield of 42.3 t/ha, Malin white – 39.8 t/ha, and Legend – 37.1 t/ ha. The most favourable weather and climatic conditions for the production of potato tubers were for the Divo 2011 variety with a yield of 45.9 t/ha and 2013 – 45.1 t/ha. For the Legenda variety 2016, the yield of potato tubers is 40.6 t/ha and 2017 – 43.2 t/ha. Malin White 2013 is 41.4 t/ha and 2017 42.1 t/ha. The average varieties of potatoes showed a slightly lower yield on average over the years of research. However, among the varieties is allocated Nadiyna – 40.3 t/ha, Slovyanka – 37.2 t/ ha and Vera 33.8 t/ha. Among the years, the most high-yielding for the Vera variety was 2016 with a yield of 36.6 t/ha and 2017 year – 37.8 t/ha. Varieties Slovyanka and Nadiyna 2011 and 2012 with yields of 42.6 and 44.3 t/ha and 46.5 and 45.3 t/ha, respectively. Characterizing the yield of potato tubers of medium-late varieties over the years of research, there was a decrease in this indicator compared with medium-early and middle-aged varieties. However, the high yield of the varieties of Dar is allocated – 40.0 t/ha, Alladin – 33.6 t/ha and Oxamit 31.3 t/ha. Among the years, the most favourable ones were: for Oxamit and Alladin – 2011 – 33.5 and 36.5 t/ha, and 2017 – 34.1 and 36.4 t/ha, respectively. Favourable years for harvesting varieties were 2011 and 2012 with yields of 45.7 and 45.8 t/ha. Thus, the highest yield of potato tubers on average over the years of studies of medium-early varieties of 41.2-43.3 t / ha were provided by weather conditions of 2011 and 2017 years, medium-ripe varieties 41.0-41.1 - 2012 and 2011, medium- late 37,6-38,5 t / ha - 2012 and 2011, respectively.


The results of studies on the influence of forms and doses of nitrogen fertilizers (ammonium nitrate, urea, carbamide-ammonia mixture, ammonium sulfate) on the productivity of oil flax under the conditions of the western forest-steppe are given. In the traditional system of fertilizer, oil flax is not able to provide high yields and quality products. An effective solution to this is optimization of plant nutrition due to its balance, which promotes intensive plant development, makes it possible to actively and efficiently intervene in the process of harvesting and its quality. Therefore, determining the optimal fertilizer rate for oil flax in specific soil-climatic conditions is important for obtaining stable and high yields of culture. The purpose of our research was to determine the contribution of doses and forms of nitrogen fertilizers to the productivity of flaxseed oil in the soil-climatic conditions of the western forest-steppe. The research was carried out in 2016-2018 at the experimental field of the Institute of Agriculture of the Carpathian region of NAAS in five-point crop rotation with the total area of the plant growing on a gray forest surface glued type of soil In general, four forms of nitrogen fertilizers were studied: ammonia, nitrate, carbamide-ammonia mixture, urea and ammonium sulfate, which were added in doses: N30; N45 one time; and N30 + N15 under pre-sowing cultivation and in the "fir tree" phase against the background of P30K60. The total number of variants was 16. The object of research was a variety of flax oilseed crops of breeding of the Institute of Oilseeds of NAAS. The seed sowing rate is 6 mln similar seed per hectare. According to the results of three-year studies, the effect of intensification of mineral nitrogen feed and various forms of nitrogen fertilizers on the indices of the elements of productivity and yield of flaxseed oil has been established. In particular, it was found that their use had little effect on the density change of plant stem growth. The use of nitrogen fertilizers in the dose N45 on the background of P30K60 improved plant survival (killed during the vegetation of 0.33-0.42 million pp./ha, which is 5.2-6.3 % for control, where the death observed 0.58 million ppm/ha or 8.4 %)) compared to the N30 dose (0.39-0.49 million pcs/ha). In addition, the use of the dose N45 separately (N30 under cultivation + N15 in the phase of the "fir tree") also improved the survival rate of plants (5.2-5.8 % of dead plants) compared with the simultaneous application of N45 cultivation (5.6-7.1 %) The percentage of deaths grew in control (without fertilizers) - 8.4 % against the background of P30K60 - 7.0 %. The application of various forms and doses of nitrogen fertilizers led to an increase in the control of the elements of productivity. The introduction of N45 against the background of P30K60 contributed to an increase in the number of boxes on plants, according to the variants of fertilizers, this index varied from 14.6 to 18.3 pieces per plant (10.9 - on the control, 12.9 pcs/roll - on the РК background), increasing the amount of seeds per plant by 12.7 pc. per plant, a mass of 1000 seeds per 0.2-0.3 g compared with the control and 0.1-0.2 g compared with the LCD background, where this figure was 6.8 g. On average, over the years of research, the highest productivity of flax seed of oilseed Vodograi (2,27 and 2,48 t / ha) was obtained provided the mineral fertilizer N15P30K60 was applied for cultivation in combination with carbamide feed in the phase of "fir tree" at a dose of N15. When using the nitrogen component in the form of KAS, the gain to control was 1.45 t / ha, to the background - 1.12 t/ha. In control and against the background, this indicator was 1.04 and 1.36 t/ha respectively. When using other forms of nitrogen fertilizers on the background of P30K60, lower yields of flaxseed crops were obtained (1.37-1.38 t/ha). The introduction of the N45 separately (N30 under cultivation + N15 into the "fir tree" phase) generally led to higher performance than a single application for cultivation. Among the studied forms of fertilizers, urea and carbamide-ammonia also caused a slightly higher percentage of fiber content in flax stems, however, it was within the experimental error and was not significantly increased.


2019 ◽  
Vol 20 (13) ◽  
pp. 1108-1121 ◽  
Author(s):  
Miriam Dibo ◽  
Eduardo C. Battocchio ◽  
Lucas M. dos Santos Souza ◽  
Matheus D. Veloso da Silva ◽  
Bruna K. Banin-Hirata ◽  
...  

The epidemiological impact of viral diseases, combined with the emergence and reemergence of some viruses, and the difficulties in identifying effective therapies, have encouraged several studies to develop new therapeutic strategies for viral infections. In this context, the use of immunotherapy for the treatment of viral diseases is increasing. One of the strategies of immunotherapy is the use of antibodies, particularly the monoclonal antibodies (mAbs) and multi-specific antibodies, which bind directly to the viral antigen and bring about activation of the immune system. With current advancements in science and technology, several such antibodies are being tested, and some are already approved and are undergoing clinical trials. The present work aims to review the status of mAb development for the treatment of viral diseases.


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