scholarly journals Phenotypic features and analysis of genes supporting probiotic action unravel underlying perspectives of Bacillus velezensis VTX9 as a potential feed additive for swine

2021 ◽  
Vol 71 (1) ◽  
Author(s):  
Ngoc Tung Quach ◽  
Thi Hanh Nguyen Vu ◽  
Ngoc Anh Nguyen ◽  
Van The Nguyen ◽  
Thi Lien Bui ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Genetic Basis ◽  
De Novo ◽  
Antagonistic Activity ◽  
Feed Additives ◽  
Feed Additive ◽  
Probiotic Properties ◽  
Bacillus Velezensis ◽  
Phenotypic Analysis ◽  
First Time

Abstract Purpose To date, a total of 13 probiotic Bacillus species are considered as a Generally Recognized as Safe organism (GRAS) approved by the US Federal Food, Drug, and Cosmetic Act (FDCA), which are used for food and feed additives. However, Bacillus velezensis is not considered as a probiotic candidate in swine farming due to a lack of genetic basis of probiotic action-related traits. Therefore, the present study was undertaken to exploit the genetic basis underlying the probiotic traits of B. velezensis VTX9. Methods The genome sequencing of B. velezensis VTX9 was performed on a PacBio Sequel platform. The probiotic properties including biosafety, antioxidative capacity, and riboflavin and exopolysaccharide production were evaluated by using genotypic and phenotypic analysis. The secondary metabolite potentials were also predicted. Results Strain VTX9 isolated from swine feces proved some probiotic properties including resistance to 3 mM H2O2, 0.6 mM bile salt, low pH, and antipathogenic activity. The complete genome of B. velezensis VTX9 consists of a 3,985,800 bp chromosome that housed 3736 protein-coding genes and 5 plasmids with the size ranging from 7261 to 20,007 bp. Genome analysis revealed no functional genes encoding enterotoxins and transferable antibiotic resistance, which confirmed the safety of VTX9. A total of 82 genes involved in gastrointestinal stress tolerance were predicted, which has not been reported previously. The maximum production of riboflavin reached 769 ± 7.5 ng/ml in LB medium after 72 h, which was in agreement with the complete de novo riboflavin biosynthetic pathway exploited for the first time in the B. velezensis genome. Antagonistic activity against pathogenic bacteria was attributed to 10 secondary metabolites clusters. The presence of a large gene cluster involved in biosynthesis of exopolysaccharides underscored further the adhesion and biofilm-forming capabilities of VTX9 in swine intestines. Conclusion Our results revealed for the first time that B. velezensis VTX9 has the potential to be a probiotic candidate. The information provided here on the genome of B. velezensis VTX9 opens new opportunities for using B. velezensis as a feed additive for swine farming in the future.

Role of probiotic fodder additive in determining productivity and assessing the health of agricultural bird

2020 ◽  
Vol 1 (8) ◽  
pp. 38-44
Author(s):  
T. N. Lenkova ◽  
◽  
I. N. Nikonov ◽  
M. V. Selina ◽  
◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Broiler Chickens ◽  
Antagonistic Activity ◽  
Feed Additives ◽  
Farm Animals ◽  
Feed Additive ◽  
Necrotic Enteritis ◽  
Scientific Group ◽  
And Control

This article presents the results of a study on testing a promising feed supplement based on a strain of Lactobacillus plantarum, with a high antagonistic activity for campylobacter. Probiotics now refers to live microbial feed additives that improve the health and productivity of farm animals. Probiotics can improve the growth rate of broiler chickens and control or prevent intestinal diseases, including salmonellosis, necrotic enteritis, and coccidiosis Scientific and industrial experience was carried out on broilers of the Cobb XNUMX cross country from daily to XNUMX days of age. As a result of research conducted by the scientific group, it was shown that feeding broiler chickens with a probiotic feed additive based on Lactobacillus plantarum helps to increase their productivity during the entire growing cycle and have a beneficial effect on the health status of farm birds.

scholarly journals Distinct Pattern of NPY in Gastro–Entero–Pancreatic System of Goat Kids Fed with a New Standardized Red Orange and Lemon Extract (RLE)

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 449
Author(s):  
Elena De Felice ◽  
Daniela Giaquinto ◽  
Sara Damiano ◽  
Angela Salzano ◽  
Simona Fabroni ◽  
...  
Keyword(s):  
Distinct Pattern ◽  
Feed Additives ◽  
Neuroendocrine Cells ◽  
Farm Animals ◽  
Feed Additive ◽  
Standard Diet ◽  
Ph Variation ◽  
Future Studies ◽  
First Time ◽  
Immunohistochemical Analyses

The use of natural compounds as feed additive is also increasing in farm animals, thanks to the beneficial effect on both animals and consumers health. Here, we questioned whether natural extracts, such as red orange and lemon extract (RLE) rich in flavanones, anthocyanins, and other polyphenols, used as feed additives could display an effect on the neuropeptide Y (NPY) in the gastro–entero–pancreatic tract of goat kids. NPY is one of the most abundant neuropeptides in mammals, known for its orexigenic role although it is involved in many central and peripheral functions. We carried out immunohistochemical analyses on samples of abomasum, duodenum and pancreas collected from two experimental groups: one fed with standard diet and one with standard diet + RLE. For the first time we document NPY distribution in the abomasum, duodenum and pancreas of goats and observe the highest number of NPY positive cells in neuroendocrine cells of duodenum. Remarkably, upon RLE feed supplementation, NPY immunoreactive cells increased significantly in abomasal epithelium and pancreatic islets but not in duodenum, likely due to pH variation of abomasum and duodenum. Our observations represent a baseline for future studies on the interaction between neuropeptides and polyphenols, used as feed additive.

scholarly journals Functional Annotation Genome Unravels Potential Probiotic Bacillus velezensis Strain KMU01 from Traditional Korean Fermented Kimchi

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 563
Author(s):  
Sojeong Heo ◽  
Jong-Hoon Kim ◽  
Mi-Sun Kwak ◽  
Moon-Hee Sung ◽  
Do-Won Jeong
Keyword(s):  
Food Industry ◽  
Genetic Basis ◽  
Genomic Sequence ◽  
Antibiotic Resistance Genes ◽  
Genomic Analysis ◽  
Essential Amino Acids ◽  
Probiotic Properties ◽  
Bacillus Velezensis ◽  
Enterotoxin Genes ◽  
Complete Genomic

Bacillus velezensis strain KMU01 showing γ-glutamyltransferase activity as a probiotic candidate was isolated from kimchi. However, the genetic information on strain KMU01 was not clear. Therefore, the current investigation was undertaken to prove the probiotic traits of B. velezensis strain KMU01 through genomic analysis. Genomic analysis revealed that strain KMU01 did not encode enterotoxin genes and acquired antibiotic resistance genes. Strain KMU01 genome possessed survivability traits under extreme conditions such as in the presence of gastric acid, as well as several probiotic traits such as intestinal epithelium adhesion and the production of thiamine and essential amino acids. Potential genes for human health enhancement such as those for γ-glutamyltransferase, nattokinase, and bacteriocin production were also identified in the genome. As a starter candidate for food fermentation, the genome of KMU01 encoded for protease, amylase, and lipase genes. The complete genomic sequence of KMU01 will contribute to our understanding of the genetic basis of probiotic properties and allow for the assessment of the effectiveness of this strain as a starter or probiotic for use in the food industry.

scholarly journals The use of probiotics in animal feeding for safe production and as potential alternatives to antibiotics

2021 ◽  
Vol 14 (2) ◽  
pp. 319-328
Author(s):  
Mbarga M. J. Arsène ◽  
Anyutoulou K. L. Davares ◽  
Smolyakova L. Andreevna ◽  
Ermolaev A. Vladimirovich ◽  
Bassa Z. Carime ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Current Knowledge ◽  
Fish Farming ◽  
Well Being ◽  
Feed Additives ◽  
Feed Additive ◽  
Human Consumption ◽  
Growth Promoters ◽  
Suitable Alternative

Although the production of safe food for human consumption is the primary purpose for animal rearing, the environment and well-being of the animals must also be taken into consideration. Based on microbiological point of view, the production of healthy food from animals involves considering foodborne pathogens, on the one hand and on the other hand, the methods used to fight against germs during breeding. The conventional method to control or prevent bacterial infections in farming is the use antibiotics. However, the banning of these compounds as growth promoters caused many changes in animal breeding and their use has since been limited to the treatment and prevention of bacterial infections. In this function, their importance no longer needs to be demonstrated, but unfortunately, their excessive and abusive use have led to a double problem which can have harmful consequences on consumer health: Resistance to antibiotics and the presence of antibiotic residues in food. The use of probiotics appears to be a suitable alternative to overcome these problems because of their ability to modulate the immune system and intestinal microflora, and further considering their antagonistic role against certain pathogenic bacteria and their ability to play the role of growth factor (sometimes associated with prebiotics) when used as feed additives. This review aims to highlight some of the negative effects of the use of antibiotics in animal rearing as well as emphasize the current knowledge on the use of probiotics as a feed additive, their influence on animal production and their potential utility as an alternative to conventional antibiotics, particularly in poultry, pig, and fish farming.

scholarly journals Assessment of potentially probiotic properties of Lactobacillus strains isolated from chickens

2016 ◽  
Vol 19 (1) ◽  
pp. 15-20 ◽  
Author(s):  
M. Kizerwetter-Świda ◽  
M. Binek
Keyword(s):  
Hydrogen Peroxide ◽  
Bile Salt ◽  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Selection Criterion ◽  
Antagonistic Activity ◽  
Low Ph ◽  
Probiotic Properties ◽  
Necrotic Enteritis ◽  
Promoting Effect

AbstractThis study was performed in order to isolate lactobacilli from chicken droppings and to select strains with the most promising probiotic properties. Lactobacillus strains were isolated from a flock of healthy laying hens. The first selection criterion was the ability to inhibit the growth of Salmonella Enteritidis. Then the tolerance to low pH and bile salt, the ability to coaggregate with pathogenic bacteria and hydrogen peroxide production were evaluated. Four isolates showing the best antagonistic activity against Salmonella Enetritidis were selected for further research. All isolates tested tolerated low pH and bile salt, likewise all produced hydrogen peroxide. They efficiently coaggregated with C. perfringens and relatively less with E. coli. Isolate 03’04 displayed above-average results in all criteria, thus it is considered as a potential probiotic for chickens, and will be further evaluated for health promoting effect in animals. The results presented in this study confirm the strain specific probiotic properties and prove the probiotic potential of isolate 03’04. Strong antagonistic properties against C. perfringens exhibited by certain Lactobacillus strains indicate the possibility to use them as a component of probiotic supplement in necrotic enteritis of poultry.

The effects of inulin and fructo-oligosaccharide on the probiotic properties of Lactobacillus spp. isolated from human milk

2018 ◽  
Vol 73 (9-10) ◽  
pp. 367-373 ◽  
Author(s):  
Şener Tulumoğlu ◽  
Belgin Erdem ◽  
Ömer Şimşek
Keyword(s):  
Human Milk ◽  
Bile Salt ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Antagonistic Activity ◽  
Probiotic Properties ◽  
Probiotic Strains ◽  
And Control ◽  
Lactobacillus Spp

Abstract This study aims to determine the effects of inulin and fructo-oligosaccharide (FOS) on the probiotic properties of five Lactobacillus spp. isolated from human milk. Lactobacillus spp. were isolated and identified, and the growth characteristics, acid and bile salt tolerance, antagonistic effects, and cholesterol assimilation of Lactobacillus strains were investigated in the presence of inulin and FOS. Lactobacillus casei L1 was able to utilize inulin and FOS as carbon source as well as glucose even other strains were able to use, including Lactobacillus rhamnosus GG. This strain also showed high tolerance to acid and bile salt, even at pH 2.5 and 0.5% bile salt levels, respectively. Inulin and FOS promoted the antimicrobial activity of L. casei L1 against pathogenic bacteria. Cholesterol assimilation was higher than in the other and control probiotic strains in the presence inulin and FOS, which were measured as 14 and 25 mg/dL, respectively. In conclusion, L. casei L1 can use both inulin and FOS to maintain its viability both at digestive conditions and also the relevant prebiotics, and show broad antagonistic activity and cholesterol assimilation.

scholarly journals Effects of Bacillus velezensis Supplementation on the Growth Performance, Immune Responses, and Intestine Microbiota of Litopenaeus vannamei

2021 ◽  
Vol 8 ◽  
Author(s):  
Lizhu Chen ◽  
Chengjie Lv ◽  
Bin Li ◽  
Huawei Zhang ◽  
Lihua Ren ◽  
...  
Keyword(s):  
Immune Responses ◽  
Growth Performance ◽  
Litopenaeus Vannamei ◽  
Pathogenic Bacteria ◽  
Control Diet ◽  
Challenge Test ◽  
Feed Additive ◽  
Bacillus Velezensis ◽  
Final Body Weight ◽  
Gain Rate

In the present study, Bacillus velezensis (BV007, CGMCC No. 20039) was isolated from the gut of Litopenaeus vannamei, and the effects of BV007 on the growth performance, immune responses, and intestine microbiota of the shrimp were investigated. A total of 1,200 healthy shrimp (3.0 ± 0.3 cm, 0.32 ± 0.8 g) were randomly divided into four groups, and fed diets supplemented with different levels of BV007 (C: 0; BV1: 1 × 105 CFU/g; BV2: 1 × 107 CFU/g; and BV3: 1 × 109 CFU/g) for 8 weeks. The results showed a significantly increased final body length (FBL), length gain rate (LGR), final body weight (FBW), weight gain rate (WGR), plumpness index (PI), and specific growth rate (SGR) in shrimp fed with BV007 for 42 days compared with shrimp fed with control diet. The activity of α-amylase in hepatopancreas was also significantly increased in the BV007-administered groups. After 42 days of growth trial, the challenge test with Vibrio parahaemolyticus was conducted for 2 weeks. The enhanced immune responses were exhibited by shrimp fed with BV007 after V. parahaemolyticus challenge, particularly in respiratory bursts and superoxide dismutase, catalase, and alkaline phosphatase activities. Moreover, the administration of BV007 could considerably increase the abundance of potential probiotics (Bacillus) and reduced the abundances of potential pathogenic bacteria (Vibrio) in shrimp intestines. In conclusion, the dietary supplementation with B. velezensis BV007 could promote the growth performance, enhance the immune responses, and modulate the intestine microbiota of shrimp, and 107 CFU/g feed was recommended to be used as a feed additive to enhance the growth and health status of shrimp.

scholarly journals Bacillus velezensis strain MBY2, a potential agent for the management of crown gall disease

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0252823
Author(s):  
Haifa Ben Gharsa ◽  
Meriam Bouri ◽  
Amira Mougou Hamdane ◽  
Christina Schuster ◽  
Andreas Leclerque ◽  
...  
Keyword(s):  
Crown Gall ◽  
Pathogenic Bacteria ◽  
Screening Program ◽  
Plant Protection ◽  
Antagonistic Activity ◽  
Soil Conditions ◽  
Bacillus Velezensis ◽  
Crown Gall Disease

The reduction of the use chemical pesticides in agriculture is gaining importance as an objective of decision-makers in both politics and economics. Consequently, the development of technically efficient and economically affordable alternatives as, e.g., biological control agents or practices is highly solicited. Crown gall disease of dicotyledonous plants is caused by ubiquitous soil borne pathogenic bacteria of the Agrobacterium tumefaciens species complex, that comprises the species Agrobacterium fabrum and represents a globally relevant plant protection problem. Within the framework of a screening program for bacterial Agrobacterium antagonists a total of 14 strains were isolated from Tunisian soil samples and assayed for antagonistic activity against pathogenic agrobacteria. One particularly promising isolate, termed strain MBY2, was studied more in depth. Using a Multilocus Sequence Analysis (MLSA) approach, the isolate was assigned to the taxonomic species Bacillus velezensis. Strain MBY2 was shown to display antagonistic effects against the pathogenic A. fabrum strain C58 in vitro and to significantly decrease pathogen populations under sterile and non-sterile soil conditions as well as in the rhizosphere of maize and, to a lower extent, tomato plants. Moreover, the ability of B. velezensis MBY2 to reduce C58-induced gall development has been demonstrated in vivo on stems of tomato and almond plants. The present study describes B. velezensis MBY2 as a newly discovered strain holding potential as a biological agent for crown gall disease management.

scholarly journals Dietary Supplementation of Inorganic, Organic, and Fatty Acids in Pig: A Review

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1740
Author(s):  
Giulia Ferronato ◽  
Aldo Prandini
Keyword(s):  
Fatty Acids ◽  
Gastrointestinal Tract ◽  
Organic Acids ◽  
Pathogenic Bacteria ◽  
Tricarboxylic Acid Cycle ◽  
Antimicrobial Properties ◽  
Feed Additives ◽  
Feed Additive ◽  
Growth Promoter ◽  
Growth Promoters

Reduction of antibiotic use has been a hot topic of research over the past decades. The European ban on growth-promoter use has increased the use of feed additivities that can enhance animal growth performance and health status, particularly during critical and stressful phases of life. Pig farming is characterized by several stressful periods, such as the weaning phase, and studies have suggested that the proper use of feed additives during stress could prevent disease and enhance performance through modulation of the gastrointestinal tract mucosa and microbiome. The types of feed additive include acids, minerals, prebiotics, probiotics, yeast, nucleotides, and phytoproducts. This review focuses on commonly used acids, classified as inorganic, organic, and fatty acids, and their beneficial and potential effects, which are widely reported in the bibliography. Acids have long been used as feed acidifiers and preservatives, and were more recently introduced into feed formulated for young pigs with the goal of stabilizing the stomach pH to offset their reduced digestive capacity. In addition, some organic acids represent intermediary products of the tricarboxylic acid cycle (TCA), and thus could be considered an energy source. Moreover, antimicrobial properties have been exploited to modulate microbiota populations and reduce pathogenic bacteria. Given these potential benefits, organic acids are no longer seen as simple acidifiers, but rather as growth promoters and potential antibiotic substitutes owing to their beneficial action on the gastrointestinal tract (GIT).

scholarly journals PELUANG ZAT BIOAKTIF TANAMAN SEBAGAI ALTERNATIF IMBUHAN PAKAN ANTIBIOTIK PADA AYAM / The Opportunities of Plants Bioactive Compound as an Alternative of Antibiotic Feed additive on Chicken

2019 ◽  
Vol 38 (2) ◽  
pp. 96
Author(s):  
Tiurma - Pasaribu
Keyword(s):  
Bioactive Compounds ◽  
Feed Efficiency ◽  
Pathogenic Bacteria ◽  
Negative Impact ◽  
Raw Materials ◽  
Animal Health ◽  
Bioactive Compound ◽  
Feed Additives ◽  
Feed Additive ◽  
Bioactive Substances

<p>Bioactive compounds (phenols, tannins, flavonoids, essential oils, curcumin, saponins, phyllanthin) have the ability as an antibacterial or antifungal. Feed additives are feed raw materials that do not contain nutrients, however, it may increase productivity, quality of livestock products (meat, eggs, milk, skin, feathers), the feed efficiency and to improve animal health or resistance of disease. Feed additives that are widely used in the livestock industry include antibiotics, antioxidants, antifungals, emulsifiers, and binders. The aim of using antibiotics is to reduce the population of pathogenic microbes or disturbing microbes in the digestive tract. Antibiotics have been banned for used because it can cause resistance to pathogenic bacteria or intestinal microflora which has a negative impact on consumers. To improve feed efficiency in poultry and to produce higher quality products, healthy and safe for consumption, the antibiotic could be replaced with plant bioactive compound. The aims of this review is to describe the role of plant bioactive compounds as feed additive to replace antibiotic for chickens. Some of plant bioactive substances that can be used as feed additives include phenols, curcumin, saponins, tannins, phenols, flavonoids, alkaloids. Bioactive substances from plants have several functions including inhibiting the growth of bacteria or fungi, increasing endurance, as an adjuvant, and preventing fat oxidation. It can be concluded that bioactive substances from plants have potential as feed additives which have the ability as antibacterial, antifungal, antioxidant, immunostimulator, and adjuvant.</p><p>Keywords: bioactive compound, plants, feed additives, chicken </p><p> </p><p><strong>Abstrak</strong></p><p> Zat bioaktif (fenol, tanin, flavonoid, minyak atsiri, curcumin, saponin, phyllanthin) memiliki kemampuan sebagai antibakteri atau antifungi. Imbuhan pakan adalah bahan baku pakan yang tidak mengandung nutrisi, namun dapat meningkatkan produktivitas, kualitas produk ternak (daging, telur, susu, kulit, bulu), efisiensi penggunaan pakan dan meningkatkan kesehatan hewan atau ketahanan terhadap penyakit. Imbuhan pakan yang banyak digunakan dalam industri peternakan termasuk antibiotik, antioksidan, antifungi, pengemulsi, dan pengikat (binder). Tujuan penggunaan antibiotik adalah untuk mengurangi populasi mikroba patogen atau mikroba yang mengganggu di saluran pencernaan. Antibiotik telah dilarang untuk digunakan karena dapat menyebabkan resistensi terhadap bakteri patogen atau mikroflora usus yang memiliki dampak negatif pada konsumen. Untuk meningkatkan efisiensi pakan pada unggas dan menghasilkan produk berkualitas tinggi, sehat dan aman untuk dikonsumsi, antibiotik dapat diganti dengan zat bioaktif tanaman. Tujuan dari ulasan ini adalah untuk menggambarkan peran zat bioaktif tanaman sebagai pengganti imbuhan pakan antibiotik pada ayam. Beberapa zat bioaktif tanaman yang dapat digunakan sebagai imbuhan pakan termasuk fenol, kurkumin, saponin, tanin, fenol, flavonoid, alkaloid. Zat bioaktif dari tanaman memiliki beberapa fungsi antara lain menghambat pertumbuhan bakteri atau jamur, meningkatkan daya tahan tubuh, sebagai bahan adjuvan dan mencegah oksidasi lemak. Dapat disimpulkan bahwa zat bioaktif dari tanaman berpotensi sebagai imbuhan pakan yang memiliki kemampuan sebagai antibakteri, antifungi, antioksidan, imunostimulator, dan adjuvant.</p><p>Kata kunci: Zat bioaktif, tanaman, imbuhan pakan, ayam </p>

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