scholarly journals The protective effect of Ocimum sanctum leaf extract against lead acetate-induced nephrotoxicity and hepatotoxicity in mice (Mus musculus)

2021 ◽  
Vol 14 (1) ◽  
pp. 250-258
Author(s):  
Wiwik Misaco Yuniarti ◽  
Nina Krismaharani ◽  
Priska Ciptaningsih ◽  
Kristania Celia ◽  
Anwar Ma'ruf ◽  
...  
Keyword(s):  
Protective Effect ◽  
Lead Acetate ◽  
Leaf Extract ◽  
Tween 80 ◽  
Negative Control Group ◽  
Negative Control ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Hydropic Degeneration

Background and Aim: The liver and kidneys are the most sensitive organs to lead exposure. Drugs that inhibit the actions of lead in the liver and kidneys are required to protect them from such an exposure. This study investigates the protective effect of the leaf extract of Ocimum sanctum (OS) against lead acetate-induced nephrotoxicity and hepatotoxicity in mice. Materials and Methods: A total of 20 male mice were divided into five equal groups for the 24-day testing period. The negative control group was administered Tween-80 1% orally for 24 days. The positive control group was administered Tween-80 1% orally for 24 days and, starting on day 4, 20 mg/kg BW lead acetate orally once a day for 21 days 1 h after the administration of Tween-80 1%. The other three treatment groups were administered BW OS leaf extract orally in the amount of 140, 280, and 560 mg/kg once a day for 24 days and, starting on day 4, 20 mg/kg BW lead acetate orally for 21 days 1 h after the administration of OS leaf extract. On day 25, the mice were sacrificed to assess the levels of blood urea nitrogen (BUN), creatinine, malondialdehyde (MDA), serum glutamic-oxaloacetic transaminase (SGOT), and serum glutamic-pyruvic transaminase (SGPT) as well as the histopathological changes. Results: The OS leaf extract caused a decrease in the scores for hepatocyte degeneration and portal inflammation (p<0.05) but not for hepatic necrosis (p>0.05) in mice exposed to lead. Similar patterns were observed in the effect of OS leaf extract on the renal morphofunction. The OS leaf extract decreased the scores for hydropic degeneration, tubular necrosis, and glomerular necrosis. The levels of MDA, SGOT, SGPT, BUN, and creatinine decreased in the lead-exposed mice treated with OS leaf extract (p<0.05). Conclusion: The administration of OS leaf extract has a protective effect against lead acetate-induced hepatotoxicity and nephrotoxicity in mice.

scholarly journals Protective Effect of Propolis Extract Against Lead Acetate Toxicity in Mice (Mus Musculus) Testes

2017 ◽  
Vol 3 (6) ◽  
pp. 557
Author(s):  
Tuti Widawati ◽  
Sri Agus Sudjarwo ◽  
Herry Agoes Hermadi
Keyword(s):  
Protective Effect ◽  
Lead Acetate ◽  
Tween 80 ◽  
Positive Control ◽  
Negative Control ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Oxygen Species ◽  
Propolis Extract ◽  
Hematoxylin Eosin

This study was aimed to investigate the protective effect of propolis extract from Apis mellifera that was obtained from Agro Tawon Rimba Raya Malang against the exposure of lead acetate 20 mg/kgBW orally. Twenty-five BALB/C mice were randomly devided into five groups. Negative control received only CMC-Na 1.5 %  and Tween 80 0.5% also aquadest an hour after the first administration; Positive control group that administered CMC-Na 1.5 %  and Tween 80 0.5% then continued received 20 mg/kgBW of lead acetate, Treatment group was received 200; 400 and 800 mg/kgBW of propolis first and then an hour after that received 20 mg/kgBW of lead acetate. The treatment was conducted for 7 days of adaptation and 35 days of treatment. At the end of the research all mice were sacrificed and testes were collected. Testes tissue were processed using Hematoxylin-Eosin staining. The result showed that an increase of spermatocyte, spermatid and sertoli cell compare to group that only received lead acetate, but thickness of seminiferous tubules epithelium showed slighly similar in all groups. Key words  : propolis, lead, reactive oxygen species, free radical, testes

scholarly journals Multi Floral Honey Has a Protective Effect against Mammary Cancer Induced by 7,12-Dimethylbenz(a)anthracene in Sprague Dawley Rats

2017 ◽  
Vol 9 (2) ◽  
pp. 196 ◽  
Author(s):  
Hamed R. Takruri ◽  
Maha S. Shomaf ◽  
Saida F. Shnaigat
Keyword(s):  
Protective Effect ◽  
Mammary Cancer ◽  
Histopathological Examination ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Light Cycle ◽  
Sprague Dawley ◽  
Dark Light

This research was conducted to study the protective effect of bee honey on the 7,12-dimethylbenz(a)anthracene (DMBA)- induced breast cancer in rat model. The study consisted of three groups: honey group, positive control group (PC), and negative control group (NC) to which the carcinogen was not administered. All rats were fed the diet recommended by the American Institute of Nutrition for growing rats (AIN-93G), with addition of honey (50 g/kg diet) to the honey group. All Rats were fed their diets ad libitum on 12 hours dark/light cycle. At the age of 50 days all rats in the honey and PC groups were gavaged once by the carcinogen DMBA with a dose of 80 mg/kg body Wt. After three weeks of carcinogen administration, rats were palpated weekly to detect any tumor growth. After 18 weeks, all rats were sacrificed. The palpable structures and the mammary glands along with associated lymph nodes were removed and fixed in saline formalin and prepared for histopathological examination. The results revealed that the honey group diet significantly (p < 0.05) reduced the incidence rate of mammary cancer, palpable tumor multiplicity, tumor size and weight compared to the PC group. In conclusion, multi floral honey has a protective effect against DMBA- induced mammary cancer in the initiation, promotion, and progression stages of DMBA-induced mammary carcinogenesis. However, further research is needed to reveal the mechanisms that might have contributed to the preventive effect of honey against mammary cancer.

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

Effects of 1% and 3% Mobe Leaf Extract Gel on Socket Wound Healing after Tooth Extraction

2021 ◽  
Vol 24 (1) ◽  
pp. 1-5
Author(s):  
Olivia Avriyanti Hanafiah ◽  
Denny Satria ◽  
Avi Syafitri
Keyword(s):  
Wound Healing ◽  
Tooth Extraction ◽  
Leaf Extract ◽  
Alveolar Bone ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
P Value ◽  
Gel Treatment

Tooth extraction is a process of removing teeth from the alveolar bone. In wound healing, fibroblast are very important cells. The main purpose of this study was to determine the effect of mobe leaf 1% and 3% extract gel (Artocarpus lakoocha) on fibroblast proliferation in post extraction tooth socket wound healing. This research used 16 samples of wistar rats, divided into 4 groups, a positive control group, a negative control group and a 1% and 3% mobe leaf extract gel group. The left mandibular incisors were extracted, then 1% and 3% gels of mobe leaf extract were applied on day 1 to day 7. Data analysis was calculated using the Kruskal-Wallis test on clinical data and one way ANOVA test for microscopic. The result of the socket wound healing activity test for a good concentration of mobe leaf extract gel was 3%. This research shows significant resultith p-value of 0.018 (< 0.05) on the closure of the socket wound clinically which means the closure of the wound accelerates because of the mobe leaf 3% extract gel treatment. The distance of fibroblast on microscopically shows significant resultith a p-value of 0.002 (< 0.05), which means that there was an enlargement of the distance fibroblast at the socket wound closure with application of mobe leaf 3% extract gel. From the results of the study it can be concluded that mobe leaf 3% extract gel has the best ability to show acceleration the closure of the socket wound either clinically or microscopically.

scholarly journals ANTI-TRYPANOSOMA ACTIVITY OF ETHANOLIC EXTRACT OF NEEM LEAF (Azadirachta indica) ON Trypanosoma evansi IN RATS (Rattus norvegicus)

2017 ◽  
Vol 11 (1) ◽  
pp. 27-30
Author(s):  
Yudha Fahrimal ◽  
Siti Maghfirah ◽  
Rinidar Rinidar ◽  
Al Azhar ◽  
Nuzul Asmilia ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Trypanosoma Evansi ◽  
Male Rats ◽  
Control Group ◽  
Neem Leaf Extract

The aim of this study was to determine the effect of neem leaf extract (Azadirachta indica) on parasitemia of rats infected with Trypanosoma evansi (T. evansi) Aceh local isolate. A total of 24 male rats aged three months were used in this study and randomly divided into six treatment groups equally. The negative control group (K0) without T. evansi infection and neem leaf extract, the positive control group (K1) was infected with T. evansi but no neem leaf extract given, group K2, K3, K4, and K5 were infected with 5x104 T. evansi and were given neem leaf extract after patent infection with dose of 50, 100, 400, and 800 mg/kg BW respectively. The extract was given orally for three consecutive days. On the fourth day, rat blood was drawn for parasitemia examination. The results showed that no T. evansi detected in rats in negative control group (K0), while parasitemia in group K1; K2; K3; K4; and K5 was 12,295 x106/mL; 10,495 x106/mL; 9,360 x106/mL; 5,080x106/mL; and 2,398x106/mL of blood, respectively. Percentage of inhibition of parasitemia in K2, K3, K4, and K5 reached 14.64, 23.78, 58.68, and 80.50%, respectively. Based on the result of the study, neem leaf extract of 800 mg/kg BW gave the highest reduction of parasitemia in rats infected with T. evansi.

scholarly journals Tulsi (Ocimum sanctum) Leaf Ethanol Extract Reduces Inflammatory Cell Infiltration in Aspirin-Induced Gastritis Rats

2020 ◽  
Vol 31 (1) ◽  
pp. 49
Author(s):  
Festi Artika Sari ◽  
Willy Sandhika ◽  
Tri Hartini Yuliawati
Keyword(s):  
Treatment Group ◽  
Rat Model ◽  
Leaf Extract ◽  
Inflammatory Cell ◽  
Negative Control ◽  
Inflammatory Cell Infiltration ◽  
Male Rats ◽  
Cell Infiltration ◽  
Ocimum Sanctum ◽  
Control Group

<p class="ISIABSTRAKINGGRIS">Gastritis is an inflammation of the gastric mucosa. Tulsi leaf extract has phenol, flavonoid and saponin compounds which are potential as antioxidant and increase defensive factors in the gastric. The purpose of this research was to find out the effect of tulsi (Ocimum sanctum) leaf extract in polymorphonuclear (PMN) inflammatory cell infiltration in gastric of aspirin-induced gastritis rat model. This study was laboratory experimental research using post-test only control group design. Randomly, 27 male rats were divided into 3 groups, the first group was not induced by aspirin and extract as negative control, the second group was induced by aspirin of 600 mg/kgBW as positive control, and the third group was induced by aspirin of 600 mg/kgBW and was given Ocimum sanctum extract at a dose of 400 mg/kgBW as treatment group. Gastric of the rats were taken on 16th day for histopathology evaluation using hematoxylin and eosin (HE) staining. Evaluation was done by calculating the PMN inflammatory cell infiltration in mucosal and submucosal layer. The results of the average number of PMN inflammatory cell in the gastric tissue of the treatment group showed a significant decrease compared to the positive and negative control groups with P-value &lt;0.05. This study proved that Ocimum sanctum leaf extract administration with the dose of 400 mg/kgBW can decrease gastritis inflammation by reducing PMN inflammatory cell in gastric of aspirin-induced gastritis rat model.</p>

scholarly journals PENGARUH EKSTRAK DAUN GEDI (Abelmoschus manihot L.) TERHADAP GAMBARAN HISTOPATOLOGI AORTA TIKUS WISTAR DENGAN DIET ATEROGENIK

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Nadya Nadiratika Papodi
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Foam Cells ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Blood Cholesterol ◽  
Control Group ◽  
Pork Lard ◽  
Pork Fat

Abstract: High blood cholesterol is a major risk factor for atherosclerosis. Gedi plants contain polyphenolic compounds that can lowering blood cholesterol levels. This study aimed to find out the effect of gedi (Abelmoschus manihot L) leaves extract on histopatologic features of aorta of those wistar rats, which had been administered an atherogenic diet. This was an experimental study on animal using 20 Wistar rats as samples. The rats were divided into 4 different groups, 5 rats for each group. Negative control group was fed only pelet, positive control group was fed pork fat as much as 2 ml/day, group C was fed on pork fat and gedi leaf extract, and group D was fed on pork fat then continued with gedi leaf extract. The results indicated that administration of 30 mg gedi leaf extract showed a histopathologic feature which is decreasing of foam cells. Conclusion: Microscopically, aorta of those rats fed on pork lard diet showed a number of foam cells in tunica intima and media whereas the rats that were fed on pork lard along with gedi leaf extract, generally there were no foam cells, but there aresome parts which contain few foam cells. On the group of Wistar rats which were fed on pork lard, continued with gedi leaf extract showed less foam cells compared to the positive control group.Keywords: Gedi leaf extract, pork lard, foam cell.Abstrak: Kadar kolesterol darah yang tinggi merupakan faktor risiko utama terjadinya aterosklerosis. Tumbuhan gedi mengandung senyawa polifenol yang dapat menurunkan kadar kolesterol darah. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun gedi terhadap gambaran histopatologi aorta tikus wistar dengan diet aterogenik. Hewan uji yang digunakan berupa 20 ekor tikus Wistar yang dibagi ke dalam 4 kelompok, masing-masing kelompok terdiri dari 5 ekor tikus. Kelompok kontrol negatif hanya diberikan pelet biasa, kelompok kontrol positif diberikan diet lemak babi 2ml/hari, kelompok C diberikan diet lemak babi bersamaan dengan ekstrak daun gedi, dan kelompok D diberikan lemak babi kemudian dilanjutkan dengan pemberian ekstrak daun gedi. Hasil penelitian memperlihatkan bahwa pemberian ekstrak daun gedi 30 mg mampu memberikan gambaran histopatologi aorta berupa berkurangnya jumlah sel busa. Simpulan: Secara mikroskopik, aorta tikus wistar yang diberi lemak babi menunjukkan adanya penumpukan sel-sel busa pada tunika intima dan media, sedangkan pada aorta tikus wistar yang diberi lemak babi bersamaan dengan ekstrak daun gedi maupun yang dilanjutkan dengan ekstrak daun gedi ditemukan berkurangnya jumlah sel-sel busa.Kata Kunci : Ekstrak daun gedi, Lemak babi, Sel busa.

scholarly journals Protective Effect of Combination Commercial Black Seed Oil (Nigella sativa) and Honey Against Cisplatin-Induced Hepatotoxicity in Rats

2020 ◽  
Vol 1 (2) ◽  
pp. 43
Author(s):  
Andri Muhrim Siddiq ◽  
Muhammad In'am Ilmiawan ◽  
Mitra Handini
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Rat Liver ◽  
Liver Tissue ◽  
Seed Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Black Seed ◽  
Black Seed Oil

Background: The chemotherapeutic use of cisplatin (CP) is restricted because of its hepatotoxicity induced by oxidative stress. Malondialdehyde (MDA) is a secondary product of lipid peroxidation as a biomarker of oxidative stress. Individual administration of black seed oil (BSO) or honey (H) demonstrated hepatoprotective effect in rats. Interaction of both substances when administrated as combination can be evaluated using combination index (CI) to quantitatively depict synergism (CI<1), additive (CI=1) and antagonism effect (CI>1). Objective: to know the combination effect of BSO and honey on rat liver tissue given CP exposure. Methods: This study used 30 rats were divided into 10 groups. Normal group (N); Negative control group (NC); P1-P4 groups were administerated BSO (1 and 2 mL/kg) and honey (3.7 and 7.4 mL/kg); P5-P8 groups were combination of BSO and H. P1-P8 groups were given BSO and honey orally for 21 days. On the 18th day, NC and P1-P8 groups were given CP 8 mg/kg intraperitoneally, while the N group was given NaCl 0.9% 1 mL/kg intraperitoneally. Result: Malondialdehyde (MDA) levels were found to be lower in P1-P8 groups compared to negative control group and P6 and P7 groups have levels equivalent to MDA levels of normal control group (p > 0.05). Conclusion: Combination of BSO and honey provides a protective effect on cisplatin-induced rat liver tissue damage indicated by reduced MDA levels, but all combination group showed antagonism effect.

scholarly journals The effect of broccoli (Brassica oelracea var botrytis) extract to the microanatomy structure of liver and kidney in mice (Mus musculus L.) after exposed by lead acetate (Pb-acetate) orally

2006 ◽  
Vol 4 (1) ◽  
pp. 14-21
Author(s):  
RIRIN DIYAH SETYANINGSIH ◽  
NOOR SOESANTI HANDAJANI ◽  
MARTI HARINI
Keyword(s):  
Mus Musculus ◽  
Lead Acetate ◽  
Negative Control Group ◽  
Negative Control ◽  
Proximal Tubules ◽  
Control Group ◽  
Average Weight ◽  
Central Vein ◽  
Damage Level ◽  
Liver And Kidney

The aims of this research were to know the effect of broccoli extract to the microanatomy structure of liver and kidney in mice after induction of lead acetate. Broccoli contains antitoxic compound such as sulforaphane, beta-carotene, indole, quercetin, and glutathione. The research was evaluated experimentally to Swiss mice at the age of 2 months with average weight of 31 g. These mice were divided into five groups. A group was a placebo controls that given only with distilled water 1 ml for 30 days. Negative control group received lead acetate orally at the dose of 0.52 mg/g body weights for 15 days. Mice of group 1, 2, and 3 received the same treatment but continued with giving broccoli extract orally in three doses: 2.1 mg; 2.8 mg and 3.5 mg/g bw/day respectively for 15 days. Liver and kidney were removed at 31st day. Observation of microanatomy structure was: 1) Determining the kind and damage level of hepatocyte, glomerulus and proximal tubules epithelia as the qualitative data; 2) Measuring the height of proximal tubules epithelia, glomerulus diameter and central vein diameter as the quantitative data, which then analyzed with ANOVA. The result of this research showed that exposed by lead acetate orally caused hydrophilic degeneration, fatty swelling and necrotic (pycnotic, karyorrhexis, and karyolysis) on hepatocyte and proximal tubules epithelia, swelling of glomeruli. Consumption of broccoli at the dose of 3.5 mg/g bw/day could repair the damage cells of lead acetate. Broccoli extract at the lowest dose (2.1 mg/g bw/day and 2.8 mg/g bw/day) could not repair the damage cells of lead acetate. Consumption of broccoli extract at the dose of 3.5 mg/g bw/day was helping the cells repairmen (i.e. hepatocyte, glomerulus and proximal tubule epithelia) in mice (Mus musculus. L) damage by lead acetate.

scholarly journals Potensi Ekstrak Daun Nangka sebagai Biolarvasida Nyamuk Culex sp

2018 ◽  
Vol 16 (1) ◽  
Author(s):  
Asro Abdi Firdaus ◽  
Setiawan . ◽  
Ferry Kriswandana
Keyword(s):  
Leaf Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Treatment Groups ◽  
Plant Parts ◽  
Post Test ◽  
Post Hoc ◽  
Culex Sp

ABSTRACTMosquitoes are type of insect that acts as a disease vector. One of them is Culex sp mosquitos which their existence is many around us. In Indonesia there are still a lot of disease cases that caused by Culex sp mosquitos, one of them is filariasis disease. The efforts to control thecurrent population of Culex sp mosquitoes mostly use chemical insecticides. The control becomes difficult because of the resistance to chemical insecticides and adversely affects the environment. This study aims to determine the potential of jackfruit leaf extract as biolarvasida against Culex sp. The type of this study was post test onlycontrolled group design, using 625 larvae Culex sp instar III, divided into 5 groups (negative control group, 0.25% extract, 0.50% extract, 0.75% extract, extract and 1% extract). Observation was performed 24 hoursafter the treatment and counted the number of larvae death.The data were analyzed by Kruskal Wallis test followed by Post Hoc Mann-Whitney U test to determine the killing power of leafy leaf extractusing Probit analysis. The results of data analysis in this study were thedeath rate percentage of larvae after 24 hours treatment was 0% incontrol, 44% in 0.25%extract, 52% in0.50% extract, 60% in 0.75%extract,while in the extract group of 1%, the number of larval deaths was 72%. Itobtained results with significant differencein some treatment groups thatwere concentrations of 0.25% - 1%. Probit analysis showed that LC50 wasat 0.382% concentration.From results of the studyit can be concluded that jackfruit leaf extract(Artocarpus heterophyllus Lamk) was potential as Culex sp mosquitobiolarvacide. So it is necessary to conduct further research on the effectsof compounds that contained in other plant parts such as flowers, stemsand roots which will be expected to have a function asbiolarvasida.Keywords: Leaf Jackfruit, Biolarvasida, Mosquito Culex sp

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