The New Detection Method of Ovarian Follicle Development Using Digitized Wide Area Measurement

METHODS: The research method used in this research was experimental laboratory with pre-and posttest only control group design. RESULTS: The result shows that the extradiol level which has range of 26.30–31.03 from 28 experimental animals measured, this showed more measurement diameter which has not had measurement addition compare with the wide percentage of measurement. The result shows strong correlation between digitalized measured wide follicles to the changing of estradiol level with value of 0.453. The result of comparation between estradiol level and measured diameter shows weak correlation. This shows that manual measurement of follicle diameter still weak to the changing of estradiol level. CONCLUSION: There is strong correlation between measured wide area follicle used ImageJ applications to the changing of estradiol level compare to the measurement of follicle diameter.

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Effect of eCG applied to dairy cows in the postpartum period on ovarian activity and reproductive performance

Medycyna Weterynaryjna ◽

10.21521/mw.6364 ◽

2020 ◽

Vol 76 (03) ◽

pp. 6364-2020

Author(s):

KUDRET YENİLMEZ ◽

NURULLAH ÖZDEMİR

Keyword(s):

Dairy Cows ◽

Postpartum Period ◽

Ovarian Follicle ◽

Control Group ◽

Ovarian Activity ◽

Serum Estradiol ◽

Serum Progesterone ◽

Ovarian Follicle Development ◽

Follicle Diameter ◽

Equine Chorionic Gonadotropin

The aim of this study was to determine the effect of equine chorionic gonadotropin (eCG) applied in postpartum (pp) period on ovarian follicle development, serum estradiol concentration, serum progesterone concentration, the calving-to-first oestrus (calving-to-first insemination) interval, and conception at first insemination. The material of this study consisted of 20 cows on day 14 pp. Cows included in this study were randomly divided into two groups. Cows in the first group, were treated with eCG (500 IU Folligon; Intervet, Holland) for 14 days. No treatment was applied to the second group, and it was left as a control group, Ovarian follicle diameters were measured by ultrasonographic examination on days 14, 16, 18, 20 and 22 pp in all animals included in the study. Serum estradiol and progesterone were from blood samples taken on the same days. All animals included in the study were followed up until the first observable oestrus was detected, and they were inseminated 12 hours after the appearance of oestrus symptoms. A pregnancy examination was performed on the 45th day after insemination. In cows administered with eCG during postpartum period, an increase in ovarian follicle diameters compared to untreated cows and a decrease in the calving-to-first oestrus interval were determined. In contrast, serum estradiol and progesterone hormone levels did not differ between the two groups. Pregnancy rate in the experimental group was higher than that in the control group, but there was no statistical difference between them. As a result, it was concluded that eCG applied to dairy cows on day 14 pp did not affect serum estradiol and progesterone levels, but it affected the ovarian follicle diameter and the calving-to-first oestrus interval positively.

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Uji Sensitisasi Dermal Masker Gel Kombinasi Ekstrak Ampas Daun Teh dan Air Cucian Beras

Jurnal Kefarmasian Indonesia ◽

10.22435/jki.v9i1.491 ◽

2019 ◽

pp. 57-64

Author(s):

Andi Nurul Annisa ◽

Wahyu Utaminingrum ◽

Erza Genatrika

Keyword(s):

Oryza Sativa L ◽

Sample Selection ◽

Challenge Test ◽

Simple Random Sampling ◽

Control Group ◽

Control Group Design ◽

Experimental Laboratory ◽

Delayed Hypersensitivity Reaction ◽

Tea Leaf ◽

Washing Water

The combination of tea leaf dregs extract and rice washing water as a peel-off gel mask was found to be stable up to 28 days of storage and had an IC50 value 20.4 ppm. This potential can provide an optimal effects as cosmetic ingredients.However, to meet the regulatory requirements of Badan Pengawas Obat Makanan (BPOM) RI and ISO 10993, a dermal sensitization test also need to be done. The aim of this study was to determine the dermal sensitization reaction from peel-off gel mask containing a combination of tea leaf dregsextract (Camellia sinensis L.) and rice washing water (Oryza sativa L.) in test animal. Thisstudy was designed based on experimental laboratory methods and sample selection by simple random sampling. The intervention was given based on posttest-only control group design. The test results for dermal sensitization were determined using the Guinea Pig Maximization Test (GPMT) method. The results showed erythema and edema reactions according to the Magnusson and Kligman scale after administration of peel-off gel masks as samples in the topical tests in test animals, appeared on the 11th day and in the challenge test appeared on the 24th day. This was due to incubation period for one week where the hypersensitivity state can develop, so that this reaction is also called the delayed hypersensitivity reaction

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Hyperbaric Oxygen (HBO) Reduce Expression of Hypoxia Inducible Factor-1 Alpha (HIF-1 alpha) and Endometriotic Tissue Size in Mice Model of Endometriosis

E3S Web of Conferences ◽

10.1051/e3sconf/202015101003 ◽

2020 ◽

Vol 151 ◽

pp. 01003

Author(s):

Dedy Syahrizal ◽

Cut Mustika ◽

Teuku Renaldi ◽

Mohammad G. Suryokusumo ◽

Hendy Hendarto

Keyword(s):

Hyperbaric Oxygen ◽

Hypoxia Inducible Factor ◽

Control Group ◽

Mice Model ◽

Control Group Design ◽

Hypoxia Inducible Factor 1 ◽

Peritoneal Tissue ◽

Implant Size ◽

Experimental Laboratory ◽

Tissue Size

Hypoxia in endometriosis will increase the expression of Hypoxia Inducible Factor-1alpha (HIF1alpha) and its expression could be decreased by Hyperbaric Oxygen (HBO). This study aimed to analyze the effect of HBO 2.4 ATA for 3x30 minutes per day for 10 days on the expression of HIF-1 alpha and endometriotic tissue size on mice model of endometriosis. This study was an experimental laboratory study with a separate pretest-posttest control group design. The mice were divided into three groups, the first was a pretest control group (which describes the condition after endometrium transplantation), the second was the endometriotic group that received hyperbaric oxygen, and the third was the endometriotic group that did not receive hyperbaric oxygen therapy. The endometriosis implant size in the peritoneal tissue was assessed and the immunohistochemistry examination was conducted to determine the expression of HIF-1 alpha. The endometriosis tissue size was reduced in the HBO group compared to the control and nonHBO group. The lowest expression of HIF-1 alpha was significantly found in HBO over the other group. The decrease of HIF1 alpha expression mediates the reduction of size endometriotic tissue due to the therapy of HBO.

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JUMLAH ION KROMIUM (Cr) DAN NIKEL (Ni) KAWAT ORTODONTIK STAINLESS STEEL YANG TERLEPAS DALAM PERENDAMAN SALIVA

e-GIGI ◽

10.35790/eg.3.2.2015.10577 ◽

2015 ◽

Vol 3 (2) ◽

Author(s):

Ciendy O. Jura ◽

Lydia E. N. Tendean ◽

P. S. Anindita

Keyword(s):

Stainless Steel ◽

Oral Cavity ◽

Artificial Saliva ◽

Steel Wire ◽

Stainless Steel Wire ◽

Control Group ◽

Ion Release ◽

Control Group Design ◽

Orthodontic Wire ◽

Experimental Laboratory

Abstract: Orthodontic stainless steel wire is one of the instrument components oftenly used in orthodontic treatment and may persist in the oral cavity for a long period of time. Orthodontic stainless steelwire in the oral cavity can be corrosive in the presence of Cr and Ni ion release which can be harmful to the human body and the stainless steel wire itself. This study aimed to determine the amount of Cr and Ni ions released from the stainless steel orthodontic wire that was immersed in artificial saliva. This was an experimental laboratory study with a posttest only control group design. Samples were analyzed by using a UV-Vis spectrophotometry to determine the released Cr and Ni ions in the saliva. Samples consisted of 4 brands of orthodontic stainless steel wires immersed in artificial saliva for 30 days with a temperature of 370C. Data were analyzed by using a computer program. The results showed that the release of Cr ions in samples A, B, C, and D respectively were: 0.302 ppm, 0.331ppm, 0,311 ppm, and 0.483 ppm meanwhile of Ni ions were 1.930 ppm, 1.778 ppm, 1.654 ppm, and 1.391ppm. Conclusion: The release of Cr and Ni ions varied in each sample of orthodontic stainless steel wire .Keywords: orthodontic stainless steel, Cr, Ni, artificial saliva, UV-Vis spectrophotometryAbstrak: Kawat ortodontik stainless steel merupakan salah satu komponen alat yang sering digunakan dalam perawatan ortodontik dan dapat bertahan dalam rongga mulut untuk jangka waktu yang lama. Kawat ortodontik stainless steel yang berada di dalam rongga mulut dapat mengalami korosi dengan adanya pelepasan ion Cr dan Ni yang bersifat merugikan bagi tubuh manusia dan kawat ortodontik itu sendiri. Penelitian ini bertujuan untuk mengetahui besarnya jumlah pelepasan ion Cr dan Ni dari kawat ortodontik stainless steel yang direndam dalam saliva buatan. Penelitian ini menggunakan metode eksperimental laboratorium dengan rancangan posttest only control group. Sampel diuji dengan menggunakan spektrofotometri UV-Vis untuk mengetahui pelepasan ion Cr dan Ni dalam saliva. Sampel terdiri dari 4 merek kawat ortodontik stainless steel direndam dalam saliva buatan selama 30 hari dengan suhu 370C. Data hasil penelitian dianalisis menggunakan program komputer. Hasil penelitian menunjukkan pelepasan ion Cr pada sampel A, B, C, dan D berturut-turut 0,302 ppm; 0,331 ppm; 0,311 ppm; dan 0,483 ppm sedangkan pelepasan ion Ni 1,930 ppm; 1,778 ppm; 1,654 ppm; dan 1,391 ppm. Simpulan: Pelepasan ion Cr dan Ni bervariasi dari masing-masing sampel kawat ortodontik stainless steel.Kata kunci : Kawat ortodontik stainless steel, Cr, Ni, saliva buatan, spektrofotometri UV-Vis

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Pengaruh Masase pada Punggung Terhadap Intensitas Nyeri Kala I Fase Laten Persalinan Normal Melalui Peningkatan Kadar Endorfin

Jurnal Kesehatan Andalas ◽

10.25077/jka.v4i1.193 ◽

2015 ◽

Vol 4 (1) ◽

Author(s):

Yeni Aryani ◽

Masrul Masrul ◽

Lisma Evareny

Keyword(s):

Pain Intensity ◽

Strong Correlation ◽

Statistical Test ◽

T Test ◽

Control Group ◽

Intensity Level ◽

Control Group Design ◽

Normal Delivery ◽

Group Design ◽

Post Test

AbstrakNyeri saat persalinan merupakan proses yang fisiologis. Sebanyak 12% - 67% wanita merasa khawatir dengan nyeri yang akan dialami saat persalinan. Salah satu upaya untuk mengurangi nyeri persalinan adalah dengan masase. Tujuan Penelitian ini untuk mengetahui pengaruh masase pada punggung terhadap intensitas nyeri kala I fase laten persalinan normal melaui peningkatan kadar endorfin. Ini merupakan suatu penelitian experimental dengan post test only control group design yang dibagi atas kelompok perlakuan yang melakukan masase pada punggung dan kelompok kontrol yang tidak masase. Intensitas nyeri dinilai dengan kuisioner dan kadar endorfin diukur dengan human beta endorfin Elisa Kit. Data dianalisis menggunakan uji t-test independent dan korelasi Spearmen. Hasil penelitian ini ditemukan ibu bersalin yang dimasase memiliki intensitas nyeri lebih rendah 29.62 point dari pada yang tidak dimasase nilai p=0.001, ada pengaruh masase terhadap intensitas nyeri kala I persalinan normal. Ibu bersalin yang dimasase memiliki endorfin lebih tinggi dari pada yang tidak dimasase sebesar 142.82 pcg/mlnilai p=0.001 ada pengaruh masase terhadap kadar endorfin ibu bersalin normal. Ada korelasi kadar endorfin dengan penurunan intensitas nyeri dengan nilai r= 0,795 dan nilai p=0.001. Kesimpulan penelitian ini adalah masase pada punggung berpengaruh terhadap intensitas nyeri dan kadar endorfin ibu bersalin kala I fase laten persalinan normal serta kadar endorfin berkorelasi dengan intensitas nyeri kala I fase laten persalinan normal.Kata kunci: masase pada punggung, intensitas nyeri, kadar endorfin.AbstractPain in delivery is a physiological process. About 12% - 67% of women feel the pain during delivery. One of the ways to reduce pain during delivery process is to massage mother’s back. The objective of this research was to determine the effect of massage on the back to the pain intensity in normal delivery based on the level of endorphin. This study was an experimental study with post test only control group design by massaging mother’s back in normal delivery process of primiparous phase I for 30 minutes. Data collection was done for three months. The subject were choosen randomly block with 52 respondents. Data processing was done based on the Independent t-test and Spearmen Correlation. The results of this study shows that mothers who have massage on the back before delivery process, feel lower pain intensity in 29.62 points than those who do not have massage. The result of statistical test was p =0.001, so, the massage reduces the pain intensity. Mothers who have massage, get their endorphin increased as much as 142.82 PCG/ml compared to mothers who did not have massage. There was a correlation between the increase of endorphin level with the reduction of pain intensity. The result of statistical test shows that there is a strong correlation between the endorphin level with pain intensity with a value,r=0.795andp=0.001. It can be conluded that massage on the back can reduce pain intensity in normal delivery and increase the leve lof endorphin. Furthemore, there is a strong correlation between the increase of endorphin level with pain intensity in normal delivery. It is recommended that massage on the back can be done regularly in every normal delivery. Keywords: massage on the back, pain intensity, level of endorphin

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Perbedaan daya antiglukan NaOCl 2,5% dan ekstrak kulit manggis (Garcinia mangostana L.) 0,09% terhadap Enterococcus faecalis (Comparison of antiglucan activity between NaOCl 2.5% and mangosteen pericarp extract (Garcinia mangostana Linn) 0.09% against Enterococcus faecalis)

Conservative Dentistry Journal ◽

10.20473/cdj.v7i1.2017.1-5 ◽

2019 ◽

Vol 7 (1) ◽

pp. 1

Author(s):

Nabiela Rahardia ◽

M Rulianto ◽

Dian Agustin Wahjuningrum

Keyword(s):

Enterococcus Faecalis ◽

Root Canal ◽

Bacterial Biofilm ◽

Control Group ◽

Garcinia Mangostana ◽

Control Group Design ◽

Root Canals ◽

Viable Cells ◽

Experimental Laboratory ◽

The Difference

Background. Failure of endodontic treatment is commonly caused by the persistent microorganisms remaining in the root canal such as Enterococcus faecalis. Enterococcus faecalis can form a biofilm in tough environmental conditions within the root canals and caused biofilm-mediated infections which needs more complicated treatment due to the increasing of antimicrobial resistance. The biofilm formation initial and most important step is bacteria adherence to the solid surface that is mediated by glucan. NaOCl 2.5% is a commonly used root canal medicaments but can cause injury of periapical tissue. Mangosteen pericarp extract contains flavonoid, tannin, and xanthone have mechanism for inhibiting adherence of bacterial biofilm. Difference of antibacterial activity between NaOCl 2.5% and mangosteen pericarp extract 0.09% can be determined by experimental laboratory to determine the adherence of bacteria in each treatment. Purpose. The aim of this study was to assess the difference of antiglucan activity between NaOCl 2.5% and mangosteen pericarp extract 0.09% on Enterococcus faecalis. Method. This study was designed as an experimental laboratory study with post test only control group design using Enterococcus faecalis ATCC 29212. Mangosteen pericarp was extracted using maceration method. Adherence analysis was observed after 24 hours by examining the viable cells in suspension. These viable cells are measured by UV-Vis spectrophotometer to compare the suspensions’ turbidity. Using the Independent T-Test, significantly less bacteria were found adhering to the mangosteen pericarp extract. Results. Absorbancy difference level by mangosteen pericarp extract 0.09% is significantly greater than the NaOCl 2.5% (p<0.05). Conclusion. Antiglucan activity that generated by mangosteen pericarp extract 0.09% is greater than NaOCl 2.5%

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Gambaran Histopatologi Pankreas Tikus Putih (Rattus norvegicus) Pasca Perlakuan Iskemia-Reperfusi Ginjal

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v13i1.2019.58-66 ◽

2019 ◽

Vol 13 (1) ◽

pp. 58

Author(s):

Elva Rosiana ◽

Wiwit Ade Fidiawati ◽

Darmawi Darmawi

Keyword(s):

Tissue Damage ◽

Control Treatment ◽

Laboratory Research ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

White Rats ◽

Post Test ◽

Experimental Laboratory ◽

Histopathologic Feature

Ischemic-reperfusion injury is defined by a condition of hypoperfusion in the spesific organ followed by reperfusion(reoxygenation) inducing tissue damage. Tissue damage produces reactive oxygen species (ROS) leading to oxidativestress condition. Oxidative stress mediate the lipid peroxidation reactions, harm the cell and finally facilitate the celldeath. The aim of this study was to evaluate the histopathologic feature of white rat’s pancreas post renal ischemiareperfusion.This study was an experimental laboratory research with post-test-only control group design including 20male white rats. Rats were divided into 5 groups, control, treatment 1 (45 minutes ischemia), treatment 2 (45 minutesischemia followed by 1 hour reperfusion), treatment 3 (45 minutes ischemia followed by 2 hours reperfusion) andtreatment 4 (45 minutes ischemia followed by24 hours reperfusion). We found the change of the histopathologicalcharacteristics of white rat’s pancreas in term of edema, leukocyte infiltration and vacuolization.

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JUS SEMANGKA MENURUNKAN NEUTROFIL TIKUS JANTAN GALUR WISTAR YANG TERPAPAR ASAP ROKOK

Media Gizi Indonesia ◽

10.20473/mgi.v11i2.166-174 ◽

2018 ◽

Vol 11 (2) ◽

pp. 166

Author(s):

Rizky Prihandari ◽

Lailatul Muniroh

Keyword(s):

Cigarette Smoke ◽

Hazardous Substances ◽

Male Rats ◽

Laboratory Research ◽

Control Group ◽

Control Group Design ◽

Treatment Groups ◽

Wistar Strain ◽

Post Test ◽

Experimental Laboratory

Cigarette smoke contains many hazardous substances and free radical which affect respiratory system. Watermelon “Sugar Baby” juice contain lycopene as antiinﬂ ammation. The aim of this research was to analyze the effect of watermelon as antiinﬂ ammation toward neutrophils count in wistar strain male rats exposed by cigarette smoke. This study was an experimental laboratory research with pre and post test control group design. Sample consisted of 24 wistar strain male rats with ±150-230 grams weight and 3 months old which were divided into four groups. Control group was given placebo (aquadest) and treatment groups were given w atermelon juice with terraced quantity (1.5 ml, 2.5 ml, and 3.5 ml). Every group was given two sticks of cigarette smoked per day during two weeks then smoked with treatment during one week. Neutrophil from peripheral smear was observed by manual method and statistically analyzed using Anova Mixed Design and continued with One Way Anova. The result showed signiﬁ cant increased of neutrophil at second observation on control group (p=0.002) and treatment groups (p=0.000). Signiﬁ cance decreased also found in the treatment groups’s at third observation p=0.000. Signiﬁ cant between each group p= 0.017. There was signiﬁ cant different between control and treatment groups but there was no signiﬁ cant different between each treatment groups. This study concluded that there was decreased of neutrophil in wistar strain male rats which were given w atermelon juice 1.5 ml, 2.5 ml, and 3.5 ml during inﬂ ammation. 3.5 ml w atermelon juice which were given showed better result as antiinﬂ ammation..

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Puasa Selama Kebuntingan terhadap jumlah sel neuron Cereberum dan Cerebellum Rattus norvegicus Baru Lahir

Jurnal Ilmiah Kedokteran Wijaya Kusuma ◽

10.30742/jikw.v9i1.634 ◽

2020 ◽

Vol 9 (1) ◽

pp. 1

Author(s):

Ucik Nurul Hidayati ◽

Hermanto Tri Joewono ◽

Muhammad Miftahussurur ◽

Agus Sulistyono ◽

Martono Tri Utomo ◽

...

Keyword(s):

Rattus Norvegicus ◽

Total Sample ◽

Control Group ◽

Control Group Design ◽

Group Design ◽

Normality Test ◽

Significant Difference ◽

Experimental Laboratory ◽

Speed Up ◽

The Brain

Fasting by pregnant women causes increasing neurons in the brain. An increasing number of neuron cells will speed up information processing, so it is expected to increasing intelligence. The purpose of this study was to analyze the effect of fasting during pregnancy on the number of new cells of the cerebrum neuron and the newly born Rattus norvegicus cerebellum. The research design was true experimental laboratory posttest only with control group design. Pregnant Rattus norvegicus samples consisted of 3 groups and 1 control group with a total sample of 32 divided by 4; Xo control group without fast treatment, fasting X1 group in 1st trimester (2 days), fasting X2 group in 2nd trimester (2 days), fasting group X3 at TM 3 (2 days). Data analysis using Shapiro – Wilk normality test, followed by ANOVA test and using SPSS for Windows 23 software. The results of statistical analysis showed no significant difference in the number of cerebrum neuron cells in the four groups P = 0.210 (p> 0.05) and there were significant differences in the number of cerebrum neuron cells in the four groups P = 0.032 (p <0.05). The conclusion of this study is there was no difference in the number of neuron cells in cerebrum and cerebellum of Rattus norvegicus newborn in the fasting mother 2 days during trimester I, II dan III of pregnancy. There was a difference in the number of neuron cells in the new cerebellum of Rattus norvegicus fasting for 2 days during trimester III of pregnancy, and there were differences the effect of the number of neuron cell in cerebellum of Rattus norvegicus in all groups.

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15EFFECT OF DOSE OF ESTRADIOL VALERATE ON OVARIAN FOLLICULAR DYNAMICS IN CIDR-TREATED BEEF COWS

Reproduction Fertility and Development ◽

10.1071/rdv16n1ab15 ◽

2004 ◽

Vol 16 (2) ◽

pp. 130 ◽

Cited By ~ 3

Author(s):

R.J. Mapletoft ◽

M.G. Colazo ◽

J.A. Small ◽

D.R. Ward ◽

J.P. Kastelic

Keyword(s):

Ovarian Follicle ◽

Animal Health ◽

Beef Cows ◽

Estradiol Valerate ◽

Control Group ◽

Dominant Follicle ◽

Follicular Wave ◽

Follicle Diameter ◽

Growth Profiles ◽

Follicular Dynamics

The objective was to investigate the effect of dose of estradiol valerate (EV) on ovarian follicular growth profiles, intervals to follicular wave emergence and, following CIDR removal, estrus and ovulation in beef cows. On Day 0, 43 non-lactating, crossbred beef cows, 3 to 9 yr of age and at random stages of the estrous cycle, received a CIDR (Bioniche Animal Health; Belleville, Ontario, Canada) and were randomly allocated to one of four groups to receive no further treatment (Control; n=10), or an injection of 1mg (n=11), 2mg (n=10), or 5mg (n=12) i.m. of EV (Sigma Chemical Co, St. Louis, MO, USA) in 2mL canola oil. On Day 7, CIDR were removed and cows received 500μg i.m. of cloprostenol (Estrumate, Schering Plough Animal Health, Pointe-Claire, Quebec, Canada). Ovaries were examined by transrectal ultrasonography once daily until 48h after CIDR removal to detect ovarian follicle growth profiles, and twice daily thereafter to detect ovulation. Data were analyzed by ANOVA (LSD and Bartlett’s tests) and chi-square procedures. One cow (5mg EV group) lost the CIDR and was removed from all analyses. There was an effect of day (P<0.0001) on CL diameter, but the effects of treatment (P=0.3), and the treatment-by-day interaction (P=0.1), were not significant. Follicular wave emergence occurred within 7d in 7/10 (70%) Control cows and 31/32 (97%; P<0.04) EV-treated cows (one cow in late diestrus at the time of treatment did not respond to 1mg EV). Mean (±SD) interval from treatment to wave emergence was longer (P<0.03) in cows treated with 5mg EV (4.8±1.2d) than in those treated with 1mg (3.2±0.9 days) or 2mg EV (3.4±0.8 days), while Control cows were intermediate (3.8±2.0 days). Although follicular wave emergence tended (P<0.09) to be more synchronous in cows receiving EV, intervals from CIDR removal to estrus (P=0.7) and ovulation (P=0.8) did not differ among groups. Diameter of the dominant follicle was smaller (P<0.04) at CIDR removal and tended to be smaller (P<0.08) just prior to ovulation in the 5mg EV group (8.5±2.2 and 13.2±0.6mm, respectively) than in the Control (11.8±4.6 and 15.5±2.9mm, respectively) or 1mg EV (11.7±2.5 and 15.1±2.2mm, respectively) groups, with the 2mg EV group (10.7±1.5 and 14.3±1.7mm, respectively) intermediate. Diameter of the dominant follicle at CIDR removal was less variable (P<0.01) in the 2 and 5mg EV groups than in the Control group and intermediate in the 1mg EV group. In summary, dose of EV affected follicular dynamics, interval to and synchrony of follicular wave emergence, and dominant follicle diameter at CIDR removal and just prior to ovulation in CIDR-treated cows. However, interval from CIDR removal to estrus and ovulation was not affected by treatment. Results suggest that a dose of 2mg EV may be most efficacious in synchronizing follicular wave emergence in CIDR-treated cows.

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